atcc 33492  (ATCC)

Journal: Communications Biology

Article Title: Bacterial envelope polysaccharide cues settlement and metamorphosis in the biofouling tubeworm Hydroides elegans

doi: 10.1038/s42003-024-06585-9

Figure Lengend Snippet: Settlement and metamorphic responses from larvae of Hydroides elegans after 24 h exposure to monospecific biofilms, extracted lipopolysaccharide (LPS), O-antigen (OA), and lipid A (LA) C. lytica HI1, P. luteoviolacea HI1, T. euphilliae H1, and T. aiptasiae T48 are depicted as a boxplot. The central horizontal line represents the median, the lower and upper boundaries of the box indicate the 25th and 75th quartiles, and the whiskers extend to the 10th to 90th percentiles; n = 12. Monospecific biofilms were inoculated at 10 8 cells ml −1 , except P. luteoviolacea HI1 biofilm which was inoculated at 10 7 cells ml −1 . Extracts were tested at 4 µg ml −1 . Asterisks show significant differences from SW ( p < 0.05). WTBF wildtype biofilm, SW sterile seawater, BF biofilm.

Article Snippet: To this end, we examined the LPS from ten Gram-negative bacteria, represented by four species extracted directly from marine biofilms ( Cellulophaga lytica HI1, Thalassotalea euphilliae (two strains: H1 and H2), Pseudoalteromonas luteoviolacea (three strains: HI1, B1P, ATCC 33492) and Tenacibaculum aiptasiae T48) and three commercially available ( Escherichia coli O55:B5, Salmonella enterica serotype enteritidis and Pseudomonas aeruginosa ATCC 27316 serotype 10).

Techniques: Sterility

Journal: Communications Biology

Article Title: Bacterial envelope polysaccharide cues settlement and metamorphosis in the biofouling tubeworm Hydroides elegans

doi: 10.1038/s42003-024-06585-9

Figure Lengend Snippet: Boxplot depicting settlement and metamorphosis responses in larvae of Hydroides elegans after 24 h exposure to isolated lipopolysaccharide (LPS), O-antigen (OA), and lipid A (LA) from three strains of Pseudoalteromonas luteoviolacea (HI1, ATCC 33492, B1P). Extracts were tested at 4 µg ml −1 . The central horizontal line represents the median, the lower and upper boundaries of the box indicate the 25th and 75th quartiles, and the whiskers extend to the 10th to 90th percentiles; n = 12. Asterisks show significant differences from SW ( p < 0.05). Note: a methanol solvent control was included for lipid A samples; methanol was evaporated overnight before the addition of larvae. WTBF wildtype biofilm, SW sterile seawater.

Article Snippet: To this end, we examined the LPS from ten Gram-negative bacteria, represented by four species extracted directly from marine biofilms ( Cellulophaga lytica HI1, Thalassotalea euphilliae (two strains: H1 and H2), Pseudoalteromonas luteoviolacea (three strains: HI1, B1P, ATCC 33492) and Tenacibaculum aiptasiae T48) and three commercially available ( Escherichia coli O55:B5, Salmonella enterica serotype enteritidis and Pseudomonas aeruginosa ATCC 27316 serotype 10).

Techniques: Isolation, Solvent, Control, Sterility