frateuria aurantia atcc 33424  (ATCC)

Journal: BMC Cancer

Article Title: GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma

doi: 10.1186/s12885-018-4524-1

Figure Lengend Snippet: Widespread changes of gene expressions and critical pathways in human lung adenocarcinoma cells A549 with GMDS knockdown. a Heatmap containing 739 differentially expressed genes in human lung adenocarcinoma cell line A549 infected with lentivirus expressing either Scr-shRNA (purple) or GMDS-shRNA (red) with the criteria P < 0.05 and ▏fold change ▏ > 1.5. Genes and samples were listed in rows and columns, respectively. A colour standard for normalized expression data was shown at the bottom of the microarray heatmap (green represents downregulated genes while red represents upregulated genes). b Gene expression changes identified in microarray were confirmed using real-time quantitative PCR for selected genes CASP8, MAP3K7, CDKN1A, FAS, JUN, DDIT3, VEGFA, SKA1 and MAD2L1 in human lung adenocarcinoma cell line A549 infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA. Histogram shown here was one out of three independent experiments ( p < 0.01 ) and normalized to GAPDH. c Protein level of FAS, VEGFA, DDIT3, JUN and CDKN1A in human human lung adenocarcinoma cell line A549 infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA. GAPDH was used as internal control. d Caspase3/7 activity analysis in A549 cells and H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA. Data shown are the mean ± SEM of caspase3/7 activity from three independent experiments ( **, p < 0.01 )

Article Snippet: Primary antibodies used here were as follows: Rabbit anti-GMDS, Novus Biological, NBP1–33424 (1:500); Rabbit anti-CDKN1A, Abcam, ab7960 (1:500); Mouse anti-DDIT3, Abcam, ab11419 (1:1000); Rabbit anti-FAS, Abcam, ab82419 (1:1000); Rabbit anti-JUN, Abcam, ab32137 (1:1000); Rabbit anti-VEGFA, Abcam, ab183100 (1:500); mouse anti-Flag, Sigma, F1804 (1:1000); mouse anti-GAPDH, Santa-Cruz, sc-32,233 (1:2000).

Techniques: Infection, Expressing, shRNA, Microarray, Real-time Polymerase Chain Reaction, Activity Assay

Journal: BMC Cancer

Article Title: GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma

doi: 10.1186/s12885-018-4524-1

Figure Lengend Snippet: Modulation of CDKN1A-CDK1-mediated gene interaction network in human lung adenocarcinoma cell line A5492 cells after GMDS knockdown. Genes represented in green were downregulated while genes in red were upregulated in A549 cells after GMDS knockdown. Solid line represented direct interactions while dotted line represented indirect interactions. The meaning of abbreviations in the figure: A, activation; B, binding; C, causation/leads to; CO, correlation; CC, chemical-chemical interaction; CP, chemical-protein interaction; E, expression; EC, enzyme catalysis; I, inhibition; L, molecular cleavage; LO, localization; M, biochemical modification; miT, microRNA targeting; MB, group/complex membership; nTRR, non-targeting RNA-RNA interaction; P, phosphorylation/dephosphorylation; PD, protein-DNA binding; PP, protein-protein binding; PR, protein-RNA binding; PY, processing yields; RB, regulation of binding; RE, reaction; RR, RNA-RNA binding; T, transcription; TR, translocation; UB, ubiquitination

Article Snippet: Primary antibodies used here were as follows: Rabbit anti-GMDS, Novus Biological, NBP1–33424 (1:500); Rabbit anti-CDKN1A, Abcam, ab7960 (1:500); Mouse anti-DDIT3, Abcam, ab11419 (1:1000); Rabbit anti-FAS, Abcam, ab82419 (1:1000); Rabbit anti-JUN, Abcam, ab32137 (1:1000); Rabbit anti-VEGFA, Abcam, ab183100 (1:500); mouse anti-Flag, Sigma, F1804 (1:1000); mouse anti-GAPDH, Santa-Cruz, sc-32,233 (1:2000).

Techniques: Activation Assay, Binding Assay, Expressing, Inhibition, Modification, De-Phosphorylation Assay, Protein Binding, RNA Binding Assay, Translocation Assay