Journal: BMC Genomics

Article Title: Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

doi: 10.1186/1471-2164-13-210

Figure Lengend Snippet: Modular architecture of the array of scaffoldins identified in the A. cellulolyticus CD2 genome and their homologs from C. thermocellum ATCC 27405. Putative A. cellulolyticus scaffoldins were identified bioinformatically (see Materials and Methods for their accession numbers). Binding specificities of the indicated (black spots) cohesin and dockerin modules were determined previously [ - ]. The sca gene cluster is framed in a shaded box. All proteins have an N-terminal signal peptide except for ScaI. Acronyms: GH9, family-9 glycoside hydrolase; CBM(n), carbohydrate-binding module (family number); Cu, Copper amine oxidase; FN3, Fibronectin type III domain; Peptidase, S8 subtilisin-like peptidase; PPC, bacterial pre-peptidase C-terminal domain; Rhs, Rhs repeat domain. Accession numbers of the A. cellulolyticus scaffoldins are: [GenBank: ZP_09464033-30 (ScaA-D), ZP_09465494 (ScaE), ZP_09464236 (ScaF), ZP_09464788 (ScaG), ZP_09462752 (ScaH), ZP_09463446 (ScaI), ZP_09462222 (ScaJ), ZP_09464725 (ScaK), ZP_09464968 (ScaL), ZP_09463433 (ScaM), ZP_09463827 (ScaN), ZP_09462124 (ScaO), ZP_09461865 (ScaP)]. Accession numbers of the C. thermocellum scaffoldins are: [GenBank: CAA47840 (CipA), YP_001039467 (OlpB), ABN54275 (Orf2p), YP_001039469 (OlpA), YP_001037164 (Cthe_0736), YP_001037732 (SdbA), YP_001036883 (OlpC) and YP_001037163 (Cthe_0735)]

Article Snippet: Draft genome sequences of Acetivibrio cellulolyticus CD2 (DSM 1870, ATCC 33288) (30 Dec. 2011), and Clostridium thermocellum ATCC 27405 (16 Feb. 2007) were obtained from GenBank (accession: AEDB00000000 and CP000568, respectively).

Techniques: Binding Assay

Journal: BMC Genomics

Article Title: Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

doi: 10.1186/1471-2164-13-210

Figure Lengend Snippet: Relationship of all cohesin modules from A. cellulolyticus and C. thermocellum . Sequence-based dendrogram of cohesin modules from A. cellulolyticus (red) and C. thermocellum (blue). See scheme and key in Figure . Only significant bootstrap values are shown

Article Snippet: Draft genome sequences of Acetivibrio cellulolyticus CD2 (DSM 1870, ATCC 33288) (30 Dec. 2011), and Clostridium thermocellum ATCC 27405 (16 Feb. 2007) were obtained from GenBank (accession: AEDB00000000 and CP000568, respectively).

Techniques: Sequencing

Journal: BMC Genomics

Article Title: Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

doi: 10.1186/1471-2164-13-210

Figure Lengend Snippet: Sequence conservation pattern of dockerin modules. The two internal dockerin repeats of A. cellulolyticus (based on 137 sequences) and C. thermocellum (71 sequences) are represented by sequence logos. Positions of calcium binding residues are shown in cyan, and putative recognition residues are shown in yellow

Article Snippet: Draft genome sequences of Acetivibrio cellulolyticus CD2 (DSM 1870, ATCC 33288) (30 Dec. 2011), and Clostridium thermocellum ATCC 27405 (16 Feb. 2007) were obtained from GenBank (accession: AEDB00000000 and CP000568, respectively).

Techniques: Sequencing, Binding Assay

Journal: BMC Genomics

Article Title: Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

doi: 10.1186/1471-2164-13-210

Figure Lengend Snippet: Comparative distribution of dockerin-containing CAZyme modules in A. cellulolyticus vs. C. thermocellum

Article Snippet: Draft genome sequences of Acetivibrio cellulolyticus CD2 (DSM 1870, ATCC 33288) (30 Dec. 2011), and Clostridium thermocellum ATCC 27405 (16 Feb. 2007) were obtained from GenBank (accession: AEDB00000000 and CP000568, respectively).

Techniques: Genome Wide

Journal: BMC Genomics

Article Title: Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

doi: 10.1186/1471-2164-13-210

Figure Lengend Snippet: Summary of protein modules in cellulosomal proteins

Article Snippet: Draft genome sequences of Acetivibrio cellulolyticus CD2 (DSM 1870, ATCC 33288) (30 Dec. 2011), and Clostridium thermocellum ATCC 27405 (16 Feb. 2007) were obtained from GenBank (accession: AEDB00000000 and CP000568, respectively).

Techniques:

Journal: BMC Genomics

Article Title: Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

doi: 10.1186/1471-2164-13-210

Figure Lengend Snippet: Putative σ I and anti- σ I regulatory factors in Acetivibrio cellulolyticus CD2

Article Snippet: Draft genome sequences of Acetivibrio cellulolyticus CD2 (DSM 1870, ATCC 33288) (30 Dec. 2011), and Clostridium thermocellum ATCC 27405 (16 Feb. 2007) were obtained from GenBank (accession: AEDB00000000 and CP000568, respectively).

Techniques: