Journal: Biotechnology journal
Article Title: Rapid Communication Fast and easy protocol for the purification of recombinant S-layer protein for synthetic biology applications
doi: 10.1002/biot.201100024
Figure Lengend Snippet: (A) Addition of 50 mM CaCl2 to full-length native SbpA results in a white precipitate (top panel) that does not occur in the absence of CaCl2 (bottom panel). (B-E) Images of crystalline arrays formed by SbpA variants in solution; (B) SbpA-His7 (residues 31-1068), (C) SbpAC-trunc-His7 (residues 31-918), (D) SbpAN-trunc-His7 (residues 203-1068) and (E) native SbpA. Scale bar shows 100 nm. SbpA-His7 and native SbpA experiments were reproducible within ten different preparations. SbpAC-trunc-His7 and SbpAN-trunc-His7 were reproducible within three different preparations.
Article Snippet: ATCC American Type Culture Collection EM electron microscopy E. coli Escherichia coli His histidine Ni-NTA nitrilotriacetic acid SbpA-His 7 7 histidine-tagged SbpA containing residues 31-1068 SbpA C-trunc -His 7 C-terminally truncated SbpA containing residues 31-918 SbpA N-trunc -His 7 N-terminally truncated SbpA containing residues 203-1068 S-layer surface layer
Techniques: