Journal:
Article Title: Use of Randomly Amplified Polymorphic DNA as a Means of Developing Genus- and Strain-Specific Streptomyces DNA Probes
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Figure Lengend Snippet: RAPD fingerprint profiles and Southern (65 and 75°C wash) of Streptomyces, suprageneric, and outgroup DNA primed with 1253. (a) RAPD fingerprint profiles of Streptomyces spp. Lane 1, 1-kb ladder; lane 2, Streptomyces lividans 1326; lane 3, Streptomyces lividans TK23; lane 4, Streptomyces antibioticus; lane 5, Streptomyces albus; lane 6, Streptomyces coelicolor; lane 7, Streptomyces rimosus; lane 8, Streptomyces ambofaciens; lane 9, Streptomyces violaceus; lane 10, Streptomyces griseifuscus; lane 11, Streptomyces olivaceus; lane 12, Streptomyces badius 252; lane 13, Streptomyces lividans TK64; lane 14, Streptomyces lividans TK64.1; lane 15, Streptomyces viridosporus T7A; lane 16, Streptomyces plicatus; lane 17, Streptomyces erythraeus; lane 18, Streptomyces rochei A4; lane 19, Streptomyces strain YCED 20; lane 20, Streptomyces strain YCED 36; lane 21, Streptomyces strain YCED 64; lane 22, Streptomyces strain WYE 28; lane 23, Streptomyces strain WYE 79; lane 24, Streptomyces strain WYE 27; lane 25, Streptomyces strain WYE 98; lane 26, Streptomyces lydicus WYEC108; lane 27, Streptomyces hygroscopicus YCED 9; lane 28, Streptomyces strain YCED 33; lane 29, Streptomyces strain WYEC102; lane 30, Streptomyces strain WYEC107. (b) RAPD fingerprint profiles of suprageneric (lanes 3 to 15) and outgroup (lanes 16 to 20) strains. Lane 1, 1-kb ladder; lane 2, Streptomyces lividans 1326, positive control; lane 3, Arthrobacter ramosus; lane 4, Nocardioides simplex; lane 5, Arthrobacter globiformis 607; lane 6, Arthrobacter globiformis 8010; lane 7, Brevibacterium linens; lane 8, Micrococcus luteus; lane 9, Gordonia corallina; lane 10, Rhodococcus opacus; lane 11, Corynebacterium xerosis; lane 12, Dactylosporangium roseum; lane 13, Streptosporangium longisporum; lane 14, Microbispora aerata; lane 15, Frankia sp. strain 33255; lane 16, Burkholderia cepacia DLC 62; lane 17, Bacillus subtilis; lane 18, Pseudomonas putida mt-2; lane 19, Flavobacterium spp.; lane 20, Ralstonia eutropha JMP134. (Photograph, righthand side) Southern blot tests of homology with RAPD PCR products. The probe is a gel-purified 0.8-kb RAPD fragment generated from Streptomyces lividans 1326.
Article Snippet: Dactylosporangium roseum , Micromonosporaceae , ATCC 31744 , YEME.
Techniques: Positive Control, Southern Blot, Purification, Generated