actinosporangium vitaminophilum atcc 31673  (ATCC)

Journal: RSC Chemical Biology

Article Title: Natural Trojan horse inhibitors of aminoacyl-tRNA synthetases

doi: 10.1039/d0cb00208a

Figure Lengend Snippet: (A) Structures of albomycins, albomycin toxic moiety (SB-217452) and seryl-adenylate. (B) A scheme showing the biosynthesis, transport and intracellular processing of albomycin. IM – inner membrane, OM – outer membrane, BGC – biosynthetic gene cluster, SerRS – seryl-tRNA-synthetase. (C) The crystal structure of the FhuA outer membrane transporter in complex with albomycin (PDB ID: 1QKC ). Two conformational isomers of albomycin are shown (black arrows: 1 – extended form, 2 – compact form). A close-up view of the extended albomycin form binding site is shown, residues involved in the binding are indicated, hydrogen bonds are shown as black dashed lines. (D) The crystal structure of the FhuD periplasmic binding protein in complex with albomycin (PDB ID: 1K7S ). Residues involved in the formation of the albomycin binding site are indicated; hydrogen bonds are shown as black dashed lines. Electron density for the toxic moiety of albomycin is not observed in this structure.

Article Snippet: Presumably, Streptomyces sp. C and S. vitaminophilus ATCC 31673 produce different SB-217452-like nucleosides, since the ctj cluster lacks one of the rSAM enzymes, an ortholog of AbmJ, which is responsible for D-ribo to D-xylo epimerization of the furanose ring in albomycin.

Techniques: Binding Assay

Journal: Frontiers in Microbiology

Article Title: Comparative Genomic Analysis of Soil Dwelling Bacteria Utilizing a Combinational Codon Usage and Molecular Phylogenetic Approach Accentuating on Key Housekeeping Genes

doi: 10.3389/fmicb.2019.02896

Figure Lengend Snippet: List of genomic Nc (Nc avg ) and genomic GC3 (GC3 avg ) along with standard deviation in the 92 species of soil bacteria analyzed in this study.

Article Snippet: In the case of trpB gene, barring a few organisms, such as Bdellovibrio bacteriovorus HD100, Clostridium cadaveris NLAE-zl-G419, Streptomyces vitaminophilus ATCC 31673, Rhizobium gallicum IE4872, Acidiphilium multivorum AIU301, and Nitrobacter winogradskyi Nb-255 most of the bacteria did not have a large percentage of genes in their genome which is below the genic Nc of the remaining housekeeping genes.

Techniques: Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Comparative Genomic Analysis of Soil Dwelling Bacteria Utilizing a Combinational Codon Usage and Molecular Phylogenetic Approach Accentuating on Key Housekeeping Genes

doi: 10.3389/fmicb.2019.02896

Figure Lengend Snippet: Nc profile of the four housekeeping genes in 92 soil bacterial species.

Article Snippet: In the case of trpB gene, barring a few organisms, such as Bdellovibrio bacteriovorus HD100, Clostridium cadaveris NLAE-zl-G419, Streptomyces vitaminophilus ATCC 31673, Rhizobium gallicum IE4872, Acidiphilium multivorum AIU301, and Nitrobacter winogradskyi Nb-255 most of the bacteria did not have a large percentage of genes in their genome which is below the genic Nc of the remaining housekeeping genes.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Comparative Genomic Analysis of Soil Dwelling Bacteria Utilizing a Combinational Codon Usage and Molecular Phylogenetic Approach Accentuating on Key Housekeeping Genes

doi: 10.3389/fmicb.2019.02896

Figure Lengend Snippet: GC3 profile of the four housekeeping genes in 92 soil bacterial species.

Article Snippet: In the case of trpB gene, barring a few organisms, such as Bdellovibrio bacteriovorus HD100, Clostridium cadaveris NLAE-zl-G419, Streptomyces vitaminophilus ATCC 31673, Rhizobium gallicum IE4872, Acidiphilium multivorum AIU301, and Nitrobacter winogradskyi Nb-255 most of the bacteria did not have a large percentage of genes in their genome which is below the genic Nc of the remaining housekeeping genes.

Techniques: