k99 expression  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85

    Structured Review

    ATCC k99 expression
    Western blot of E. coli fimbrial proteins probed with different MAbs to the <t>K99</t> antigen. Panel A: Reference MAb (CVL), Panel B: MAb derived from clone 5.6.12, Panel C: MAb derived from clone 3.8.1, Lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1 2, gel filtration column fractions 1 2, respectively; 3, FPLC purified K99 antigen.
    K99 Expression, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k99 expression/product/ATCC
    Average 85 stars, based on 506 article reviews
    Price from $9.99 to $1999.99
    k99 expression - by Bioz Stars, 2022-10
    85/100 stars

    Images

    1) Product Images from "Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves"

    Article Title: Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

    Journal: Journal of Veterinary Science

    doi: 10.4142/jvs.2007.8.1.57

    Western blot of E. coli fimbrial proteins probed with different MAbs to the K99 antigen. Panel A: Reference MAb (CVL), Panel B: MAb derived from clone 5.6.12, Panel C: MAb derived from clone 3.8.1, Lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1 2, gel filtration column fractions 1 2, respectively; 3, FPLC purified K99 antigen.
    Figure Legend Snippet: Western blot of E. coli fimbrial proteins probed with different MAbs to the K99 antigen. Panel A: Reference MAb (CVL), Panel B: MAb derived from clone 5.6.12, Panel C: MAb derived from clone 3.8.1, Lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1 2, gel filtration column fractions 1 2, respectively; 3, FPLC purified K99 antigen.

    Techniques Used: Western Blot, Derivative Assay, Molecular Weight, Marker, Filtration, Fast Protein Liquid Chromatography, Purification

    Elution profile of fimbrial extracts of E. coli on a Sepharose CL-4B column (1.1 × 60 cm) equilibrated with phosphate-urea buffer. (Inset): Dot blot assay of fimbrial extracts to reference K99 monoclonal antibodies. lanes: 1, heat extract; 2, ammonium sulfate precipitate; 3, ammonium sulfate supernatant; 4 5, gel filtration column elutes 1 2, respectively.
    Figure Legend Snippet: Elution profile of fimbrial extracts of E. coli on a Sepharose CL-4B column (1.1 × 60 cm) equilibrated with phosphate-urea buffer. (Inset): Dot blot assay of fimbrial extracts to reference K99 monoclonal antibodies. lanes: 1, heat extract; 2, ammonium sulfate precipitate; 3, ammonium sulfate supernatant; 4 5, gel filtration column elutes 1 2, respectively.

    Techniques Used: Dot Blot, Filtration

    Fast protein liquid chromatography: elution profile of the E. coli K99 antigen from a cation exchange column. Column, MonoS HR 5/5; equilibration buffer, 10 mM PBS, pH 7.2; elution buffer, 10 mM PBS with 250 mM NaCl.; fraction size, 1.0 ml; flow rate, 1 ml/min.
    Figure Legend Snippet: Fast protein liquid chromatography: elution profile of the E. coli K99 antigen from a cation exchange column. Column, MonoS HR 5/5; equilibration buffer, 10 mM PBS, pH 7.2; elution buffer, 10 mM PBS with 250 mM NaCl.; fraction size, 1.0 ml; flow rate, 1 ml/min.

    Techniques Used: Fast Protein Liquid Chromatography, Flow Cytometry

    Western blot of whole cell lysates of Gram negative bacteria probed with tissue culture-derived K99 MAbs (NEK99-5.6.12). lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1, E. coli (SAR-14 strain); 2, Pasteurella ; 3, Enterobacter ; 4, Pseudomonas ; 5, Klebsiella .
    Figure Legend Snippet: Western blot of whole cell lysates of Gram negative bacteria probed with tissue culture-derived K99 MAbs (NEK99-5.6.12). lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1, E. coli (SAR-14 strain); 2, Pasteurella ; 3, Enterobacter ; 4, Pseudomonas ; 5, Klebsiella .

    Techniques Used: Western Blot, Derivative Assay, Molecular Weight, Marker

    Western blot of E. coli fimbrial proteins probed with reference K99 MAbs. Lanes: M, molecular weight marker (10 kDa protein ladder); 1, heat extract; 2, ammonium sulfate precipitate; 3 4, gel filtration column fractions 1 2, respectively; 5, FPLC purified K99 antigen.
    Figure Legend Snippet: Western blot of E. coli fimbrial proteins probed with reference K99 MAbs. Lanes: M, molecular weight marker (10 kDa protein ladder); 1, heat extract; 2, ammonium sulfate precipitate; 3 4, gel filtration column fractions 1 2, respectively; 5, FPLC purified K99 antigen.

    Techniques Used: Western Blot, Molecular Weight, Marker, Filtration, Fast Protein Liquid Chromatography, Purification

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    ATCC k99 expression
    Western blot of E. coli fimbrial proteins probed with different MAbs to the <t>K99</t> antigen. Panel A: Reference MAb (CVL), Panel B: MAb derived from clone 5.6.12, Panel C: MAb derived from clone 3.8.1, Lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1 2, gel filtration column fractions 1 2, respectively; 3, FPLC purified K99 antigen.
    K99 Expression, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k99 expression/product/ATCC
    Average 85 stars, based on 506 article reviews
    Price from $9.99 to $1999.99
    k99 expression - by Bioz Stars, 2022-10
    85/100 stars
      Buy from Supplier

    Image Search Results


    Western blot of E. coli fimbrial proteins probed with different MAbs to the K99 antigen. Panel A: Reference MAb (CVL), Panel B: MAb derived from clone 5.6.12, Panel C: MAb derived from clone 3.8.1, Lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1 2, gel filtration column fractions 1 2, respectively; 3, FPLC purified K99 antigen.

    Journal: Journal of Veterinary Science

    Article Title: Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

    doi: 10.4142/jvs.2007.8.1.57

    Figure Lengend Snippet: Western blot of E. coli fimbrial proteins probed with different MAbs to the K99 antigen. Panel A: Reference MAb (CVL), Panel B: MAb derived from clone 5.6.12, Panel C: MAb derived from clone 3.8.1, Lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1 2, gel filtration column fractions 1 2, respectively; 3, FPLC purified K99 antigen.

    Article Snippet: A standard E. coli strain positive for K99 expression (ATCC 31616) was used as positive control, and the laboratory E. coli strain HB101 was used as negative control.

    Techniques: Western Blot, Derivative Assay, Molecular Weight, Marker, Filtration, Fast Protein Liquid Chromatography, Purification

    Elution profile of fimbrial extracts of E. coli on a Sepharose CL-4B column (1.1 × 60 cm) equilibrated with phosphate-urea buffer. (Inset): Dot blot assay of fimbrial extracts to reference K99 monoclonal antibodies. lanes: 1, heat extract; 2, ammonium sulfate precipitate; 3, ammonium sulfate supernatant; 4 5, gel filtration column elutes 1 2, respectively.

    Journal: Journal of Veterinary Science

    Article Title: Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

    doi: 10.4142/jvs.2007.8.1.57

    Figure Lengend Snippet: Elution profile of fimbrial extracts of E. coli on a Sepharose CL-4B column (1.1 × 60 cm) equilibrated with phosphate-urea buffer. (Inset): Dot blot assay of fimbrial extracts to reference K99 monoclonal antibodies. lanes: 1, heat extract; 2, ammonium sulfate precipitate; 3, ammonium sulfate supernatant; 4 5, gel filtration column elutes 1 2, respectively.

    Article Snippet: A standard E. coli strain positive for K99 expression (ATCC 31616) was used as positive control, and the laboratory E. coli strain HB101 was used as negative control.

    Techniques: Dot Blot, Filtration

    Fast protein liquid chromatography: elution profile of the E. coli K99 antigen from a cation exchange column. Column, MonoS HR 5/5; equilibration buffer, 10 mM PBS, pH 7.2; elution buffer, 10 mM PBS with 250 mM NaCl.; fraction size, 1.0 ml; flow rate, 1 ml/min.

    Journal: Journal of Veterinary Science

    Article Title: Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

    doi: 10.4142/jvs.2007.8.1.57

    Figure Lengend Snippet: Fast protein liquid chromatography: elution profile of the E. coli K99 antigen from a cation exchange column. Column, MonoS HR 5/5; equilibration buffer, 10 mM PBS, pH 7.2; elution buffer, 10 mM PBS with 250 mM NaCl.; fraction size, 1.0 ml; flow rate, 1 ml/min.

    Article Snippet: A standard E. coli strain positive for K99 expression (ATCC 31616) was used as positive control, and the laboratory E. coli strain HB101 was used as negative control.

    Techniques: Fast Protein Liquid Chromatography, Flow Cytometry

    Western blot of whole cell lysates of Gram negative bacteria probed with tissue culture-derived K99 MAbs (NEK99-5.6.12). lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1, E. coli (SAR-14 strain); 2, Pasteurella ; 3, Enterobacter ; 4, Pseudomonas ; 5, Klebsiella .

    Journal: Journal of Veterinary Science

    Article Title: Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

    doi: 10.4142/jvs.2007.8.1.57

    Figure Lengend Snippet: Western blot of whole cell lysates of Gram negative bacteria probed with tissue culture-derived K99 MAbs (NEK99-5.6.12). lanes: M, molecular weight marker (10 kDa benchmark protein ladder); 1, E. coli (SAR-14 strain); 2, Pasteurella ; 3, Enterobacter ; 4, Pseudomonas ; 5, Klebsiella .

    Article Snippet: A standard E. coli strain positive for K99 expression (ATCC 31616) was used as positive control, and the laboratory E. coli strain HB101 was used as negative control.

    Techniques: Western Blot, Derivative Assay, Molecular Weight, Marker

    Western blot of E. coli fimbrial proteins probed with reference K99 MAbs. Lanes: M, molecular weight marker (10 kDa protein ladder); 1, heat extract; 2, ammonium sulfate precipitate; 3 4, gel filtration column fractions 1 2, respectively; 5, FPLC purified K99 antigen.

    Journal: Journal of Veterinary Science

    Article Title: Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

    doi: 10.4142/jvs.2007.8.1.57

    Figure Lengend Snippet: Western blot of E. coli fimbrial proteins probed with reference K99 MAbs. Lanes: M, molecular weight marker (10 kDa protein ladder); 1, heat extract; 2, ammonium sulfate precipitate; 3 4, gel filtration column fractions 1 2, respectively; 5, FPLC purified K99 antigen.

    Article Snippet: A standard E. coli strain positive for K99 expression (ATCC 31616) was used as positive control, and the laboratory E. coli strain HB101 was used as negative control.

    Techniques: Western Blot, Molecular Weight, Marker, Filtration, Fast Protein Liquid Chromatography, Purification