Review





Similar Products

hrp-conjugated secondary antibodies goat antirabbit igg #31234  (Thermo Fisher)
90
Thermo Fisher hrp-conjugated secondary antibodies goat antirabbit igg #31234
Hrp Conjugated Secondary Antibodies Goat Antirabbit Igg #31234, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp-conjugated secondary antibodies goat antirabbit igg #31234/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
hrp-conjugated secondary antibodies goat antirabbit igg #31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
secondary antibody thermofisher scientific cat#31234  (Thermo Fisher)
90
Thermo Fisher secondary antibody thermofisher scientific cat#31234
Secondary Antibody Thermofisher Scientific Cat#31234, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibody thermofisher scientific cat#31234/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
secondary antibody thermofisher scientific cat#31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
anti-cd63 antibodies sc-31234  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-cd63 antibodies sc-31234
Anti Cd63 Antibodies Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd63 antibodies sc-31234/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
anti-cd63 antibodies sc-31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
sc 31234  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology sc 31234
Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sc 31234/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
sc 31234 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
goat anti-cd81 polyclonal antibody sc-31234  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology goat anti-cd81 polyclonal antibody sc-31234
Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, <t>CD81,</t> and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.
Goat Anti Cd81 Polyclonal Antibody Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti-cd81 polyclonal antibody sc-31234/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
goat anti-cd81 polyclonal antibody sc-31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
goat anticd81 polyclonal antibody sc-31234  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology goat anticd81 polyclonal antibody sc-31234
Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, <t>CD81,</t> and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.
Goat Anticd81 Polyclonal Antibody Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anticd81 polyclonal antibody sc-31234/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
goat anticd81 polyclonal antibody sc-31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
anti-cd81 antibody sc-31234  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-cd81 antibody sc-31234
Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, <t>CD81,</t> and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.
Anti Cd81 Antibody Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd81 antibody sc-31234/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
anti-cd81 antibody sc-31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
primary antibody cd81 sc-31234  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology primary antibody cd81 sc-31234
Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, <t>CD81,</t> and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.
Primary Antibody Cd81 Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibody cd81 sc-31234/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
primary antibody cd81 sc-31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
anti-cd81 sc-31234  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-cd81 sc-31234
Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, <t>CD81,</t> and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.
Anti Cd81 Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd81 sc-31234/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
anti-cd81 sc-31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
primary antibody anti-cd81 sc-31234  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology primary antibody anti-cd81 sc-31234
Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, <t>CD81,</t> and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.
Primary Antibody Anti Cd81 Sc 31234, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibody anti-cd81 sc-31234/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
primary antibody anti-cd81 sc-31234 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, CD81, and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Extracellular Vesicles From LPS-Treated Macrophages Aggravate Smooth Muscle Cell Calcification by Propagating Inflammation and Oxidative Stress

doi: 10.3389/fcell.2022.823450

Figure Lengend Snippet: Characterization of macrophage-derived EVs. EVs were isolated from the culture media of RAW cells incubated with (EV-LPS) or without (EV-CT) 1 µg/ml lipopolysaccharide-EK (LPS-EK) for 6 h. Evaluation of EV morphology by (A) transmission electron microscopy (TEM) and (B) cryo-electron microscopy (cryo-EM). (C) Particle-size distribution and (D) the total concentration of macrophage-derived EVs were measured by nanoparticle-tracking analysis (NTA). (E) CD9, CD81, and β-actin protein expression were assessed by western blotting. (F) Quantification of the enzymatic activity of acetylcholinesterase (AChE) in macrophage-derived EVs. Control AChE activity in the assay diluent 1X-D-PBS (CT) was defined as 100%. Data are expressed as the mean ± SEM of seven independent experiments performed in triplicate ( n = 7). *** p < 0.001 vs . CT; $ p < 0.05 vs. EV-CT, Mann-Whitney test.

Article Snippet: After blocking, membranes were incubated overnight at 4°C with primary antibodies: rabbit anti-CD9 polyclonal antibody (1/1,000, GeneTex ® GTX55564), goat anti-CD81 polyclonal antibody (1/1,000, Santa Cruz Biotechnology, Inc. ® sc-31234), mouse monoclonal anti-β-actin antibody (1/5,000, Sigma-Aldrich ® A1978, clone AC-15), rabbit anti-SMPD3 (1/10,000, Sigma-Aldrich ® SAB2102245), or rabbit anti-p62/SQSTM1 (1/1,000, Sigma-Aldrich ® P0067).

Techniques: Derivative Assay, Isolation, Incubation, Transmission Assay, Electron Microscopy, Cryo-Electron Microscopy, Cryo-EM Sample Prep, Concentration Assay, Expressing, Western Blot, Activity Assay, Control, MANN-WHITNEY