Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Suppression of STK11 induces expansion of polymorphonuclear myeloid-derived suppressive cells and activation of immune signaling in breast cancer

doi: 10.1007/s00262-025-04189-8

Figure Lengend Snippet: Consequence of low STK11 in breast cancer. A Overall survival curve according to the mRNA of STK11 from The Cancer Genome Atlas Breast Invasive Carcinoma in Genomic Data Commons Data Portal (GDC TCGA-BRCA) dataset. The patients with high STK11 expression have a better survival than those with low expression ( P = 0.0230). B - D Differential expression genes (DEGs) of Stk11 -KO cells compared with their parental counterparts based on RNA sequencing analysis. B Venn diagram showing DEGs identified in Stk11 -KO versus parental cells for the three mouse breast cancer cell lines, Group 1: M158, Group 2: NF639, and Group 3: PY8119. A total of 56 DEGs were shared among all three groups. C Volcano plot illustrating the expression patterns of the 56 DEGs. DEGs in M158 cells are shown in blue, NF639 in red, and PY8119 in green. D Predicated protein–protein interaction (PPI) network of Stk11 and the 56 DEGs

Article Snippet: The M158, NF639, and PY8119 mouse cancer cells were obtained from ATCC®.

Techniques: Expressing, Quantitative Proteomics, RNA Sequencing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Suppression of STK11 induces expansion of polymorphonuclear myeloid-derived suppressive cells and activation of immune signaling in breast cancer

doi: 10.1007/s00262-025-04189-8

Figure Lengend Snippet: Gene set enrichment analysis (GSEA) of immune-related pathways in Stk11 -KO cells versus their parental counterparts. GSEA was performed using HALLMARK and BIOCARTA gene sets on RNA sequencing data from three mouse breast cancer cell lines (PY8119, M158, and NF639). The analysis compared Stk11 -KO cells with their respective parental counterparts to identify enrichment of immune-related pathways. Representative enrichment plots are shown for the following pathways: A IL6_JAK_STAT3_SIGNALING. B INFLAMMATORY_RESPONSE. C TNFA_SIGNALING_VIA_NFκB. D INTERFERON_ALPHA_RESPONSE. E TH1_TH2 _SIGNALING. F INFLAMMATORY_RESPONSE. G IL1R_PATHWAY. H TNFR2_PATHWAY

Article Snippet: The M158, NF639, and PY8119 mouse cancer cells were obtained from ATCC®.

Techniques: RNA Sequencing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Suppression of STK11 induces expansion of polymorphonuclear myeloid-derived suppressive cells and activation of immune signaling in breast cancer

doi: 10.1007/s00262-025-04189-8

Figure Lengend Snippet: Gene set enrichment analysis (GSEA) of immune-related pathways in Stk11-KO cells versus their parental counterparts using Kyoto Encyclopedia of Genes and Genomes (KEGG), REACTOME, and GENE Ontology (GO) gene sets. GESA was performed on RNA sequencing data from Stk11 -KO cells and their parental counterparts derived from three mouse breast cancer cell lines (PY8119, M158, and NF639). Enrichment analysis included gene sets from the KEGG, REACTOME, and GO databases. Representative enrichment plots are shown for the following pathways and biological functions: A CYTOKINE_CYTOKINE_RECEPTOR_INTERACTION. B TYPE_II_INTERFERON_TO_JAK_STAT_SIGNALING_PATHWAY. C IL6_FAMILY_TO_JAK_STAT_SIGNALING-PATHWAY. D INTERLEUKIN_10_ SIGNALING. ( E ) CHEMOKINE_RECEPTORS_BIND_CHEMOKINES. F Gene ontology biological process (GOBP)_REGULATION_OF_ACUTE_ INFLAMMATORY_RESPONSE. G Gene ontology molecular function (GOMF)_CHEMOKINE_ACTIVITY. H GOBP_POSITIVE_REGULATION_OF_ INFLAMMATORY_RESPONSE. I GOBP_POSITIVE_REGULATION_OF_ ANTIGEN_PROCESSING_AND_PRESENTATION

Article Snippet: The M158, NF639, and PY8119 mouse cancer cells were obtained from ATCC®.

Techniques: RNA Sequencing, Derivative Assay, Activity Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Suppression of STK11 induces expansion of polymorphonuclear myeloid-derived suppressive cells and activation of immune signaling in breast cancer

doi: 10.1007/s00262-025-04189-8

Figure Lengend Snippet: Cytokine array analysis of immune-related protein expression in Stk11 -KO mouse breast cancer cell lines and their parental counterparts. A Array map. B - D Representative cytokine array blots for PY8119 B , M158 C , and NF639 D cell lines, comparing Stk11 -KO cells with their parental counterparts. E Semiquantitative analysis of protein expression with a top tenfold change. Signal intensities were measured and expressed as fold changes relative to parental cells, based on the dot intensity from the cytokine array. The Wilcoxon rank-sum test compared the difference between Stk11 -KO and parental cells. Abbreviations: CTACK/CCL27, cutaneous T cell-attracting chemokine/C–C motif chemokine ligand 27; CXCL1/KC, chemokine (C-X-C motif) ligand 1; CXCL16, chemokine (C-X-C motif) ligand 16; IL-4, interleukin-4; M-CSF, macrophage colony-stimulating factor; MCP-2/CCL2, monocyte chemoattractant protein-1/C–C motif chemokine ligand 2; MCP-5, monocyte chemoattractant protein-5; MIP-2, macrophage inflammatory protein-2; and VEGF-A, vascular endothelial growth factor-A

Article Snippet: The M158, NF639, and PY8119 mouse cancer cells were obtained from ATCC®.

Techniques: Expressing