Journal: Molecular Medicine Reports

Article Title: Exosomes derived from baicalin‑pretreated mesenchymal stem cells mitigate atherosclerosis by regulating the SIRT1/NF‑κB signaling pathway

doi: 10.3892/mmr.2025.13491

Figure Lengend Snippet: Ba-exos inhibit NF-κB activation and alleviate inflammation in VSMCs. (A) Reverse transcription-quantitative PCR was performed to detect the expression levels of the inflammatory genes MCP-1, IL-6, VCAM-1 and ICAM-1 in VSMCs. Immunofluorescence was used to detect the (B) levels of MCP-1 and VCAM-1, and (C) NF-κB nuclear translocation in VSMCs. Western blotting was performed to detect the (D) acetylation levels of NF-κB and the (E) protein expression levels of p65 and p-p65 in VSMCs. n=3. *P<0.05, **P<0.01 and ***P<0.001. Ba-exos, exos derived from baicalin-preconditioned MSCs; exos, exosomes; ICAM-1, intercellular adhesion molecule 1; MCP-1, monocyte chemoattractant protein 1; MSC-exos, MSC-derived exos; MSC, mesenchymal stem cell; ox-LDL, oxidized low-density lipoprotein; p-, phosphorylated; VCAM-1, vascular cell adhesion molecule 1; VSMCs, vascular smooth muscle cells.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:5,000; cat. no. 10494-1-AP; Wuhan Sanying Biotechnology), p65 (1:1,000; cat. no. ab32536; Abcam), phosphorylated (p)-p65 (1:1,000; cat. no. ab239882; Abcam), acetylated p65 (cat. no. 3045s; Cell Signaling Technology, Inc.), MCP-1 (1:1,000; cat. no. 26161-1-AP; Wuhan Sanying Biotechnology), IL-6 (1:1,000; cat. no. 21865-1-AP; Wuhan Sanying Biotechnology), VCAM-1 (1:1,000; cat. no. 11444-1-AP; Wuhan Sanying Biotechnology), intercellular adhesion molecule 1 (ICAM-1; 1:1,000; cat. no. 16174-1-AP; Wuhan Sanying Biotechnology) and SIRT1 (1:1,000; cat. no. ab110304; Abcam).

Techniques: Activation Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Immunofluorescence, Translocation Assay, Western Blot, Derivative Assay

Journal: Molecular Medicine Reports

Article Title: Exosomes derived from baicalin‑pretreated mesenchymal stem cells mitigate atherosclerosis by regulating the SIRT1/NF‑κB signaling pathway

doi: 10.3892/mmr.2025.13491

Figure Lengend Snippet: Ba-exos attenuate inflammation of VSMCs by regulating SIRT1. (A) Reverse transcription-quantitative PCR was performed to detect the expression of inflammatory genes MCP-1, IL-6, VCAM-1 and ICAM-1 in VSMCs. (B) Immunofluorescence was used to detect the levels of MCP-1 and VCAM-1 in VSMCs. (C) Western blotting was performed to detect the protein levels of MCP-1, IL-6, VCAM-1, ICAM-1, p65 and p-p65 in VSMCs. n=3. *P<0.05, **P<0.01 and ***P<0.001. Ba-exos, exos derived from baicalin-preconditioned MSCs; exos, exosomes; ICAM-1, intercellular adhesion molecule 1; MCP-1, monocyte chemoattractant protein 1; MSC-exos, MSC-derived exos; MSC, mesenchymal stem cell; NC, negative control; ox-LDL, oxidized low-density lipoprotein; p-, phosphorylated; si, small interfering RNA; SIRT1, sirtuin 1; VCAM-1, vascular cell adhesion molecule 1; VSMCs, vascular smooth muscle cells.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:5,000; cat. no. 10494-1-AP; Wuhan Sanying Biotechnology), p65 (1:1,000; cat. no. ab32536; Abcam), phosphorylated (p)-p65 (1:1,000; cat. no. ab239882; Abcam), acetylated p65 (cat. no. 3045s; Cell Signaling Technology, Inc.), MCP-1 (1:1,000; cat. no. 26161-1-AP; Wuhan Sanying Biotechnology), IL-6 (1:1,000; cat. no. 21865-1-AP; Wuhan Sanying Biotechnology), VCAM-1 (1:1,000; cat. no. 11444-1-AP; Wuhan Sanying Biotechnology), intercellular adhesion molecule 1 (ICAM-1; 1:1,000; cat. no. 16174-1-AP; Wuhan Sanying Biotechnology) and SIRT1 (1:1,000; cat. no. ab110304; Abcam).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Immunofluorescence, Western Blot, Derivative Assay, Negative Control, Small Interfering RNA

Journal: Molecular Medicine Reports

Article Title: Exosomes derived from baicalin‑pretreated mesenchymal stem cells mitigate atherosclerosis by regulating the SIRT1/NF‑κB signaling pathway

doi: 10.3892/mmr.2025.13491

Figure Lengend Snippet: Ba-exos may alleviate the progression of atherosclerosis in vivo by regulating SIRT1/NF-κB. (A) Automatic biochemical analyzer was used to detect blood glucose, total cholesterol, LDL-C, HDL-C, triglycerides and uric acid levels in mouse serum. (B) Oil Red O staining (arrows indicate the locations of the lesions) and (C) Masson staining (arrows indicate the locations of the lesions) were performed to assess tissue pathology. (D) ELISA was used to detect the levels of MCP-1, IL-6, VCAM-1 and ICAM-1 in mouse serum. (E) Western blotting was used to detect the protein expression levels of SIRT1, p65 and p-p65 in mouse tissues. n=5. *P<0.05, **P<0.01 and ***P<0.001. Ba-exos, exos derived from baicalin-preconditioned MSCs; exos, exosomes; HDL-C, high-density lipoprotein-cholesterol; ICAM-1, intercellular adhesion molecule 1; MCP-1, monocyte chemoattractant protein 1; LDL-C, low-density lipoprotein-cholesterol; MSC-exos, MSC-derived exos; MSC, mesenchymal stem cell; p-, phosphorylated; SIRT1, sirtuin 1; VCAM-1, vascular cell adhesion molecule 1.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:5,000; cat. no. 10494-1-AP; Wuhan Sanying Biotechnology), p65 (1:1,000; cat. no. ab32536; Abcam), phosphorylated (p)-p65 (1:1,000; cat. no. ab239882; Abcam), acetylated p65 (cat. no. 3045s; Cell Signaling Technology, Inc.), MCP-1 (1:1,000; cat. no. 26161-1-AP; Wuhan Sanying Biotechnology), IL-6 (1:1,000; cat. no. 21865-1-AP; Wuhan Sanying Biotechnology), VCAM-1 (1:1,000; cat. no. 11444-1-AP; Wuhan Sanying Biotechnology), intercellular adhesion molecule 1 (ICAM-1; 1:1,000; cat. no. 16174-1-AP; Wuhan Sanying Biotechnology) and SIRT1 (1:1,000; cat. no. ab110304; Abcam).

Techniques: In Vivo, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Derivative Assay