voluntary running wheel activity  (TSE systems)


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    TSE systems voluntary running wheel activity
    Skeletal muscle integrity and mitochondrial biogenesis in male TG/Nrf2-Ko mice. ( A ) Grip strength of 24 wks old animals (n = 8–10). ( B ) 24 h <t>voluntary</t> <t>running</t> <t>wheel</t> <t>activity</t> of 14 wks old male mice (n = 9–10). ( C ) Skeletal muscle mRNA expression in quadriceps muscle of the myosin heavy chain ( Mhc ) fiber type markers and ( D ) mitochondrial biogenesis markers of 24 wks old animals (n = 8–10). ( E ) Representative immunoblots of PGC1α/β in quadriceps muscle from 24 wks old mice from cropped blots of the same gel using αTubulin (αTUB) as loading control (2 samples shown out of 4–5 analyzed per group). ( F ) Representative immunoblots of phospho-AMPK Thr172 and total AMPK in quadriceps muscle from 24 wks old mice from different gels processed in parallel (2 samples shown out of 4–5 analyzed per group). ( G ) Skeletal muscle (gastrocnemius) citrate synthase activity of 24 wks old animals (n = 4–8). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; ** p
    Voluntary Running Wheel Activity, supplied by TSE systems, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/voluntary running wheel activity/product/TSE systems
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    voluntary running wheel activity - by Bioz Stars, 2022-09
    94/100 stars

    Images

    1) Product Images from "Partial involvement of Nrf2 in skeletal muscle mitohormesis as an adaptive response to mitochondrial uncoupling"

    Article Title: Partial involvement of Nrf2 in skeletal muscle mitohormesis as an adaptive response to mitochondrial uncoupling

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-20901-4

    Skeletal muscle integrity and mitochondrial biogenesis in male TG/Nrf2-Ko mice. ( A ) Grip strength of 24 wks old animals (n = 8–10). ( B ) 24 h voluntary running wheel activity of 14 wks old male mice (n = 9–10). ( C ) Skeletal muscle mRNA expression in quadriceps muscle of the myosin heavy chain ( Mhc ) fiber type markers and ( D ) mitochondrial biogenesis markers of 24 wks old animals (n = 8–10). ( E ) Representative immunoblots of PGC1α/β in quadriceps muscle from 24 wks old mice from cropped blots of the same gel using αTubulin (αTUB) as loading control (2 samples shown out of 4–5 analyzed per group). ( F ) Representative immunoblots of phospho-AMPK Thr172 and total AMPK in quadriceps muscle from 24 wks old mice from different gels processed in parallel (2 samples shown out of 4–5 analyzed per group). ( G ) Skeletal muscle (gastrocnemius) citrate synthase activity of 24 wks old animals (n = 4–8). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; ** p
    Figure Legend Snippet: Skeletal muscle integrity and mitochondrial biogenesis in male TG/Nrf2-Ko mice. ( A ) Grip strength of 24 wks old animals (n = 8–10). ( B ) 24 h voluntary running wheel activity of 14 wks old male mice (n = 9–10). ( C ) Skeletal muscle mRNA expression in quadriceps muscle of the myosin heavy chain ( Mhc ) fiber type markers and ( D ) mitochondrial biogenesis markers of 24 wks old animals (n = 8–10). ( E ) Representative immunoblots of PGC1α/β in quadriceps muscle from 24 wks old mice from cropped blots of the same gel using αTubulin (αTUB) as loading control (2 samples shown out of 4–5 analyzed per group). ( F ) Representative immunoblots of phospho-AMPK Thr172 and total AMPK in quadriceps muscle from 24 wks old mice from different gels processed in parallel (2 samples shown out of 4–5 analyzed per group). ( G ) Skeletal muscle (gastrocnemius) citrate synthase activity of 24 wks old animals (n = 4–8). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; ** p

    Techniques Used: Mouse Assay, Activity Assay, Expressing, Western Blot, Whisker Assay

    2) Product Images from "An Interval of the Obesity QTL Nob3.38 within a QTL Hotspot on Chromosome 1 Modulates Behavioral Phenotypes"

    Article Title: An Interval of the Obesity QTL Nob3.38 within a QTL Hotspot on Chromosome 1 Modulates Behavioral Phenotypes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0053025

    Characterization of voluntary activity and the metabolism of skeletal muscle of Nob3.38 B/B and Nob3.38 N/N mice. (A) Voluntary activity of B/B and N/N mice detected with a running wheel over 24 h (n = 13). (B) Glucose uptake into isolated skeletal muscle under basal and insulin-stimulated conditions (upper panel) and palmitate oxidation (lower panel) in isolated skeletal muscle under basal and AICAR stimulated conditions (EDL, left panels; Soleus, right panels) of B/B and N/N mice. (C) Characterization of mitochondrial respiration by determination of oxygen consumption rate of isolated mitochondria from skeletal muscles of B/B and N/N mice (n = 4–6).
    Figure Legend Snippet: Characterization of voluntary activity and the metabolism of skeletal muscle of Nob3.38 B/B and Nob3.38 N/N mice. (A) Voluntary activity of B/B and N/N mice detected with a running wheel over 24 h (n = 13). (B) Glucose uptake into isolated skeletal muscle under basal and insulin-stimulated conditions (upper panel) and palmitate oxidation (lower panel) in isolated skeletal muscle under basal and AICAR stimulated conditions (EDL, left panels; Soleus, right panels) of B/B and N/N mice. (C) Characterization of mitochondrial respiration by determination of oxygen consumption rate of isolated mitochondria from skeletal muscles of B/B and N/N mice (n = 4–6).

    Techniques Used: Activity Assay, Mouse Assay, Isolation

    Characterization of the body weight and behavior of RCS-IX. (A) Body weight, (B) running wheel activity, (C) grip strength, and (D) preference on the O-Maze were compared between B/B and N/N allele carriers. (E) Spatial learning was assessed by the Morris Water Maze test and escape latency (left panel) and activity (right panel) were determined during acquisition (day 1–3) and reversal (day 4–5). For testing the subcongenic line RCS-IX 5 females of B/B and 7 females of N/N genotype were used. (#p
    Figure Legend Snippet: Characterization of the body weight and behavior of RCS-IX. (A) Body weight, (B) running wheel activity, (C) grip strength, and (D) preference on the O-Maze were compared between B/B and N/N allele carriers. (E) Spatial learning was assessed by the Morris Water Maze test and escape latency (left panel) and activity (right panel) were determined during acquisition (day 1–3) and reversal (day 4–5). For testing the subcongenic line RCS-IX 5 females of B/B and 7 females of N/N genotype were used. (#p

    Techniques Used: Activity Assay

    3) Product Images from "Calorie Restriction Rescues Mitochondrial Dysfunction in Adck2-Deficient Skeletal Muscle"

    Article Title: Calorie Restriction Rescues Mitochondrial Dysfunction in Adck2-Deficient Skeletal Muscle

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2022.898792

    Calorie restriction adaptations on physical performance. Results for two and four limbs grip strength tests, weight-lifting normalized by body weight and aerobic treadmill tests are shown on basal conditions before starting and at end of diet periods (A,C,E,G,H) . Two and four limbs grip strength tests and weight-lifting test without normalization (B,D,F) . (I) Voluntary wheel running activity in the automated home cage phenotyping system. Data are expressed as distance recorded in centimeters (cm). CR, calorie restriction; BM, body mass. In all cases, data represent the mean ± SD. A, B, C y D (before diet Adck2 +/+ N = 15, Adck2 +/− N = 9. After diet Adck2 +/+ N = 15, Adck2 +/− N = 13 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 7). E and F (before diet Adck2 +/+ N = 15, Adck2 N +/− = 9. After diet Adck2 +/+ N = 9, Adck2 +/− N = 7 Adck2 +/+ CR N = 8, Adck2 +/− CR N = 7). G (Adck2 +/+ N = 16, Adck2 +/− N = 9). H (Adck2 +/+ N = 8, Adck2 +/− N = 5 Adck2 +/+ CR N = 5, Adck2 +/− CR N = 5). I (Adck2 +/+ N = 7, Adck2 +/− , N = 5 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 8). One-way ANOVA test was applied. * p
    Figure Legend Snippet: Calorie restriction adaptations on physical performance. Results for two and four limbs grip strength tests, weight-lifting normalized by body weight and aerobic treadmill tests are shown on basal conditions before starting and at end of diet periods (A,C,E,G,H) . Two and four limbs grip strength tests and weight-lifting test without normalization (B,D,F) . (I) Voluntary wheel running activity in the automated home cage phenotyping system. Data are expressed as distance recorded in centimeters (cm). CR, calorie restriction; BM, body mass. In all cases, data represent the mean ± SD. A, B, C y D (before diet Adck2 +/+ N = 15, Adck2 +/− N = 9. After diet Adck2 +/+ N = 15, Adck2 +/− N = 13 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 7). E and F (before diet Adck2 +/+ N = 15, Adck2 N +/− = 9. After diet Adck2 +/+ N = 9, Adck2 +/− N = 7 Adck2 +/+ CR N = 8, Adck2 +/− CR N = 7). G (Adck2 +/+ N = 16, Adck2 +/− N = 9). H (Adck2 +/+ N = 8, Adck2 +/− N = 5 Adck2 +/+ CR N = 5, Adck2 +/− CR N = 5). I (Adck2 +/+ N = 7, Adck2 +/− , N = 5 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 8). One-way ANOVA test was applied. * p

    Techniques Used: Activity Assay

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    TSE systems voluntary running wheel activity
    Skeletal muscle integrity and mitochondrial biogenesis in male TG/Nrf2-Ko mice. ( A ) Grip strength of 24 wks old animals (n = 8–10). ( B ) 24 h <t>voluntary</t> <t>running</t> <t>wheel</t> <t>activity</t> of 14 wks old male mice (n = 9–10). ( C ) Skeletal muscle mRNA expression in quadriceps muscle of the myosin heavy chain ( Mhc ) fiber type markers and ( D ) mitochondrial biogenesis markers of 24 wks old animals (n = 8–10). ( E ) Representative immunoblots of PGC1α/β in quadriceps muscle from 24 wks old mice from cropped blots of the same gel using αTubulin (αTUB) as loading control (2 samples shown out of 4–5 analyzed per group). ( F ) Representative immunoblots of phospho-AMPK Thr172 and total AMPK in quadriceps muscle from 24 wks old mice from different gels processed in parallel (2 samples shown out of 4–5 analyzed per group). ( G ) Skeletal muscle (gastrocnemius) citrate synthase activity of 24 wks old animals (n = 4–8). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; ** p
    Voluntary Running Wheel Activity, supplied by TSE systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/voluntary running wheel activity/product/TSE systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    voluntary running wheel activity - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

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    Skeletal muscle integrity and mitochondrial biogenesis in male TG/Nrf2-Ko mice. ( A ) Grip strength of 24 wks old animals (n = 8–10). ( B ) 24 h voluntary running wheel activity of 14 wks old male mice (n = 9–10). ( C ) Skeletal muscle mRNA expression in quadriceps muscle of the myosin heavy chain ( Mhc ) fiber type markers and ( D ) mitochondrial biogenesis markers of 24 wks old animals (n = 8–10). ( E ) Representative immunoblots of PGC1α/β in quadriceps muscle from 24 wks old mice from cropped blots of the same gel using αTubulin (αTUB) as loading control (2 samples shown out of 4–5 analyzed per group). ( F ) Representative immunoblots of phospho-AMPK Thr172 and total AMPK in quadriceps muscle from 24 wks old mice from different gels processed in parallel (2 samples shown out of 4–5 analyzed per group). ( G ) Skeletal muscle (gastrocnemius) citrate synthase activity of 24 wks old animals (n = 4–8). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; ** p

    Journal: Scientific Reports

    Article Title: Partial involvement of Nrf2 in skeletal muscle mitohormesis as an adaptive response to mitochondrial uncoupling

    doi: 10.1038/s41598-018-20901-4

    Figure Lengend Snippet: Skeletal muscle integrity and mitochondrial biogenesis in male TG/Nrf2-Ko mice. ( A ) Grip strength of 24 wks old animals (n = 8–10). ( B ) 24 h voluntary running wheel activity of 14 wks old male mice (n = 9–10). ( C ) Skeletal muscle mRNA expression in quadriceps muscle of the myosin heavy chain ( Mhc ) fiber type markers and ( D ) mitochondrial biogenesis markers of 24 wks old animals (n = 8–10). ( E ) Representative immunoblots of PGC1α/β in quadriceps muscle from 24 wks old mice from cropped blots of the same gel using αTubulin (αTUB) as loading control (2 samples shown out of 4–5 analyzed per group). ( F ) Representative immunoblots of phospho-AMPK Thr172 and total AMPK in quadriceps muscle from 24 wks old mice from different gels processed in parallel (2 samples shown out of 4–5 analyzed per group). ( G ) Skeletal muscle (gastrocnemius) citrate synthase activity of 24 wks old animals (n = 4–8). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; ** p

    Article Snippet: Voluntary running wheel activity was determined by IR motion detectors (TSE Systems GmbH, Homburg, Germany).

    Techniques: Mouse Assay, Activity Assay, Expressing, Western Blot, Whisker Assay

    Characterization of voluntary activity and the metabolism of skeletal muscle of Nob3.38 B/B and Nob3.38 N/N mice. (A) Voluntary activity of B/B and N/N mice detected with a running wheel over 24 h (n = 13). (B) Glucose uptake into isolated skeletal muscle under basal and insulin-stimulated conditions (upper panel) and palmitate oxidation (lower panel) in isolated skeletal muscle under basal and AICAR stimulated conditions (EDL, left panels; Soleus, right panels) of B/B and N/N mice. (C) Characterization of mitochondrial respiration by determination of oxygen consumption rate of isolated mitochondria from skeletal muscles of B/B and N/N mice (n = 4–6).

    Journal: PLoS ONE

    Article Title: An Interval of the Obesity QTL Nob3.38 within a QTL Hotspot on Chromosome 1 Modulates Behavioral Phenotypes

    doi: 10.1371/journal.pone.0053025

    Figure Lengend Snippet: Characterization of voluntary activity and the metabolism of skeletal muscle of Nob3.38 B/B and Nob3.38 N/N mice. (A) Voluntary activity of B/B and N/N mice detected with a running wheel over 24 h (n = 13). (B) Glucose uptake into isolated skeletal muscle under basal and insulin-stimulated conditions (upper panel) and palmitate oxidation (lower panel) in isolated skeletal muscle under basal and AICAR stimulated conditions (EDL, left panels; Soleus, right panels) of B/B and N/N mice. (C) Characterization of mitochondrial respiration by determination of oxygen consumption rate of isolated mitochondria from skeletal muscles of B/B and N/N mice (n = 4–6).

    Article Snippet: Voluntary running wheel activity was recorded with an automated running wheel system (TSE).

    Techniques: Activity Assay, Mouse Assay, Isolation

    Characterization of the body weight and behavior of RCS-IX. (A) Body weight, (B) running wheel activity, (C) grip strength, and (D) preference on the O-Maze were compared between B/B and N/N allele carriers. (E) Spatial learning was assessed by the Morris Water Maze test and escape latency (left panel) and activity (right panel) were determined during acquisition (day 1–3) and reversal (day 4–5). For testing the subcongenic line RCS-IX 5 females of B/B and 7 females of N/N genotype were used. (#p

    Journal: PLoS ONE

    Article Title: An Interval of the Obesity QTL Nob3.38 within a QTL Hotspot on Chromosome 1 Modulates Behavioral Phenotypes

    doi: 10.1371/journal.pone.0053025

    Figure Lengend Snippet: Characterization of the body weight and behavior of RCS-IX. (A) Body weight, (B) running wheel activity, (C) grip strength, and (D) preference on the O-Maze were compared between B/B and N/N allele carriers. (E) Spatial learning was assessed by the Morris Water Maze test and escape latency (left panel) and activity (right panel) were determined during acquisition (day 1–3) and reversal (day 4–5). For testing the subcongenic line RCS-IX 5 females of B/B and 7 females of N/N genotype were used. (#p

    Article Snippet: Voluntary running wheel activity was recorded with an automated running wheel system (TSE).

    Techniques: Activity Assay

    Calorie restriction adaptations on physical performance. Results for two and four limbs grip strength tests, weight-lifting normalized by body weight and aerobic treadmill tests are shown on basal conditions before starting and at end of diet periods (A,C,E,G,H) . Two and four limbs grip strength tests and weight-lifting test without normalization (B,D,F) . (I) Voluntary wheel running activity in the automated home cage phenotyping system. Data are expressed as distance recorded in centimeters (cm). CR, calorie restriction; BM, body mass. In all cases, data represent the mean ± SD. A, B, C y D (before diet Adck2 +/+ N = 15, Adck2 +/− N = 9. After diet Adck2 +/+ N = 15, Adck2 +/− N = 13 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 7). E and F (before diet Adck2 +/+ N = 15, Adck2 N +/− = 9. After diet Adck2 +/+ N = 9, Adck2 +/− N = 7 Adck2 +/+ CR N = 8, Adck2 +/− CR N = 7). G (Adck2 +/+ N = 16, Adck2 +/− N = 9). H (Adck2 +/+ N = 8, Adck2 +/− N = 5 Adck2 +/+ CR N = 5, Adck2 +/− CR N = 5). I (Adck2 +/+ N = 7, Adck2 +/− , N = 5 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 8). One-way ANOVA test was applied. * p

    Journal: Frontiers in Physiology

    Article Title: Calorie Restriction Rescues Mitochondrial Dysfunction in Adck2-Deficient Skeletal Muscle

    doi: 10.3389/fphys.2022.898792

    Figure Lengend Snippet: Calorie restriction adaptations on physical performance. Results for two and four limbs grip strength tests, weight-lifting normalized by body weight and aerobic treadmill tests are shown on basal conditions before starting and at end of diet periods (A,C,E,G,H) . Two and four limbs grip strength tests and weight-lifting test without normalization (B,D,F) . (I) Voluntary wheel running activity in the automated home cage phenotyping system. Data are expressed as distance recorded in centimeters (cm). CR, calorie restriction; BM, body mass. In all cases, data represent the mean ± SD. A, B, C y D (before diet Adck2 +/+ N = 15, Adck2 +/− N = 9. After diet Adck2 +/+ N = 15, Adck2 +/− N = 13 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 7). E and F (before diet Adck2 +/+ N = 15, Adck2 N +/− = 9. After diet Adck2 +/+ N = 9, Adck2 +/− N = 7 Adck2 +/+ CR N = 8, Adck2 +/− CR N = 7). G (Adck2 +/+ N = 16, Adck2 +/− N = 9). H (Adck2 +/+ N = 8, Adck2 +/− N = 5 Adck2 +/+ CR N = 5, Adck2 +/− CR N = 5). I (Adck2 +/+ N = 7, Adck2 +/− , N = 5 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 8). One-way ANOVA test was applied. * p

    Article Snippet: Additionally, voluntary running wheel capacity was also examined over 3 days in a TSE PhenoMaster system.

    Techniques: Activity Assay