Journal: Nature protocols
Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
doi: 10.1038/nprot.2009.27
Figure Lengend Snippet: Example of grid screens. ( a ) A broad screen set around an initial crystallization condition identified through a nanoliter-scale screening of sparse matrix screens. The condition generating the initial hit is placed close to the center of the screen,
Article Snippet: Use the mosquito Crystal nanoliter liquid handler to dispense 100 nl of protein, 100 nl of the optimized crystallization condition from the 96-well plate and 25 nl of each additive or detergent onto a protein crystallization cover for a 96-well plate at room temperature.
Techniques: Crystallization Assay