hrog36 cell lines  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH hrog36 cell lines
    The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and <t>HROG36</t> ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.
    Hrog36 Cell Lines, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrog36 cell lines/product/CLS Cell Lines Service GmbH
    Average 90 stars, based on 1 article reviews
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    Images

    1) Product Images from "Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves"

    Article Title: Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

    Journal: Foods

    doi: 10.3390/foods10010018

    The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and HROG36 ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.
    Figure Legend Snippet: The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and HROG36 ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.

    Techniques Used: Metabolic Assay, Concentration Assay

    Calculated EC 50 of extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’ and of some phenolic compounds found in the extracts for C6 and  HROG36  cells.
    Figure Legend Snippet: Calculated EC 50 of extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’ and of some phenolic compounds found in the extracts for C6 and HROG36 cells.

    Techniques Used:

    The values of coefficient for correlation between viability (metabolic activity) of C6 or  HROG36  cells and amount of phenolic compounds in the extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’.
    Figure Legend Snippet: The values of coefficient for correlation between viability (metabolic activity) of C6 or HROG36 cells and amount of phenolic compounds in the extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’.

    Techniques Used: Activity Assay

    hrog36 cell lines  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH hrog36 cell lines
    The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and <t>HROG36</t> ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.
    Hrog36 Cell Lines, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrog36 cell lines/product/CLS Cell Lines Service GmbH
    Average 90 stars, based on 1 article reviews
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    Images

    1) Product Images from "Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves"

    Article Title: Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

    Journal: Foods

    doi: 10.3390/foods10010018

    The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and HROG36 ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.
    Figure Legend Snippet: The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and HROG36 ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.

    Techniques Used: Metabolic Assay, Concentration Assay

    Calculated EC 50 of extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’ and of some phenolic compounds found in the extracts for C6 and  HROG36  cells.
    Figure Legend Snippet: Calculated EC 50 of extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’ and of some phenolic compounds found in the extracts for C6 and HROG36 cells.

    Techniques Used:

    The values of coefficient for correlation between viability (metabolic activity) of C6 or  HROG36  cells and amount of phenolic compounds in the extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’.
    Figure Legend Snippet: The values of coefficient for correlation between viability (metabolic activity) of C6 or HROG36 cells and amount of phenolic compounds in the extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’.

    Techniques Used: Activity Assay

    hrog36  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH hrog36
    Representative images of <t>HROG36,</t> C6, and A375 cell spheroid cultures on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h from spheroid positioning. Scale bar is 100 μm.
    Hrog36, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrog36/product/CLS Cell Lines Service GmbH
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrog36 - by Bioz Stars, 2024-05
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    Images

    1) Product Images from "Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels"

    Article Title: Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2020.00773

    Representative images of HROG36, C6, and A375 cell spheroid cultures on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h from spheroid positioning. Scale bar is 100 μm.
    Figure Legend Snippet: Representative images of HROG36, C6, and A375 cell spheroid cultures on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h from spheroid positioning. Scale bar is 100 μm.

    Techniques Used:

    Migration of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cell migration was evaluated by measuring the area of cell spreading around the spheroid in 24 h (A) , by counting cells in the area (B) , and by indicating cell number per mm 2 (C) . The experiments were performed in three independent experimental sets, each of three replicates. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP hydrogels, ∧ – with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.
    Figure Legend Snippet: Migration of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cell migration was evaluated by measuring the area of cell spreading around the spheroid in 24 h (A) , by counting cells in the area (B) , and by indicating cell number per mm 2 (C) . The experiments were performed in three independent experimental sets, each of three replicates. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP hydrogels, ∧ – with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.

    Techniques Used: Migration

    Metabolic activity of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h (A) and 48 h (B) . The metabolic activity of the cells corresponding to the proliferation intensity was assessed by Presto Blue TM assay in three independent experimental sets, each of three replicates. RFU, relative fluorescence units. *Indicates a statistically significant difference compared with the samples of the same cell type grown on CLP-only containing hydrogels, ∧ -with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.
    Figure Legend Snippet: Metabolic activity of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h (A) and 48 h (B) . The metabolic activity of the cells corresponding to the proliferation intensity was assessed by Presto Blue TM assay in three independent experimental sets, each of three replicates. RFU, relative fluorescence units. *Indicates a statistically significant difference compared with the samples of the same cell type grown on CLP-only containing hydrogels, ∧ -with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.

    Techniques Used: Activity Assay, Fluorescence

    Representative images of focal adhesions formed by HROG36 (A) , C6 (B) , and A375 (C) cells on CLP, CLP-RGD, and CLP-IKVAV hydrogels. The cells were immunostained for actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Scale bar is 10 μm.
    Figure Legend Snippet: Representative images of focal adhesions formed by HROG36 (A) , C6 (B) , and A375 (C) cells on CLP, CLP-RGD, and CLP-IKVAV hydrogels. The cells were immunostained for actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Scale bar is 10 μm.

    Techniques Used:

    Size distribution of individual focal contacts (A) , projected cell area, cell shape index, and number of focal contacts (B) of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cells were immunostained for F-actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Size distribution of focal contacts of three cells on each hydrogel was plotted together with approximate distribution curve. Projected cell area and perimeter for cell shape index calculation were measured by ImageJ software. Focal adhesion number per cell was calculated according to the number of vinculin-actin colocalization points. The data are presented as AVG ± SDEV of 5–7 cells from three of each hydrogel sample. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP, ∧ – compared with samples on PEG-CLP-RGD. ANOVA with post hoc LSD test, p < 0.05.
    Figure Legend Snippet: Size distribution of individual focal contacts (A) , projected cell area, cell shape index, and number of focal contacts (B) of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cells were immunostained for F-actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Size distribution of focal contacts of three cells on each hydrogel was plotted together with approximate distribution curve. Projected cell area and perimeter for cell shape index calculation were measured by ImageJ software. Focal adhesion number per cell was calculated according to the number of vinculin-actin colocalization points. The data are presented as AVG ± SDEV of 5–7 cells from three of each hydrogel sample. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP, ∧ – compared with samples on PEG-CLP-RGD. ANOVA with post hoc LSD test, p < 0.05.

    Techniques Used: Software

    Correlations between cell adhesion/morphology parameters and cell migration/proliferation.
    Figure Legend Snippet: Correlations between cell adhesion/morphology parameters and cell migration/proliferation.

    Techniques Used: Migration

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    CLS Cell Lines Service GmbH hrog36 cell lines
    The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and <t>HROG36</t> ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.
    Hrog36 Cell Lines, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrog36 cell lines/product/CLS Cell Lines Service GmbH
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrog36 cell lines - by Bioz Stars, 2024-05
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    CLS Cell Lines Service GmbH hrog36
    Representative images of <t>HROG36,</t> C6, and A375 cell spheroid cultures on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h from spheroid positioning. Scale bar is 100 μm.
    Hrog36, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrog36/product/CLS Cell Lines Service GmbH
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrog36 - by Bioz Stars, 2024-05
    90/100 stars
      Buy from Supplier

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    The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and HROG36 ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.

    Journal: Foods

    Article Title: Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

    doi: 10.3390/foods10010018

    Figure Lengend Snippet: The effect of the extracts from leaves of different Japanese quince cultivars ( a , b ) and some phenolic compounds found in the extracts ( c , d ) on viability of glioblastoma C6 ( a , c ) and HROG36 ( b , d ) cells evaluated by metabolic activity assay with PrestoBlue reagent. For Ethanol, the concentrations were the same as in other samples at the indicated concentration point, i.e., 35, 50, 65, 80, 95, 110, 125, and 150 L/mL. In both ( a , c ), * indicates significant difference compared with ethanol-only treated samples; in ( a ), ^ significant difference compared with temozolomide; in ( c ), ^ significant difference compared with Epicatechin, + with Chlorogenic acid, # with Hyperoside. In ( b ), all extract-treated samples were statistically significantly different from Ethanol starting from concentration 0.25 mg/mL, and Temozolomide–starting from 0.5 mg/mL. in ( d ), all the samples were statistically significantly different from Ethanol starting from the concentration of 7 g/mL, and there was statistically significant difference between Epicatechin and other samples at 7 and 9 g/mL. The level of significance p < 0.05.

    Article Snippet: The C6 and HROG36 cell lines were purchased from the Cell Lines Service GmbH (Germany).

    Techniques: Metabolic Assay, Concentration Assay

    Calculated EC 50 of extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’ and of some phenolic compounds found in the extracts for C6 and  HROG36  cells.

    Journal: Foods

    Article Title: Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

    doi: 10.3390/foods10010018

    Figure Lengend Snippet: Calculated EC 50 of extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’ and of some phenolic compounds found in the extracts for C6 and HROG36 cells.

    Article Snippet: The C6 and HROG36 cell lines were purchased from the Cell Lines Service GmbH (Germany).

    Techniques:

    The values of coefficient for correlation between viability (metabolic activity) of C6 or  HROG36  cells and amount of phenolic compounds in the extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’.

    Journal: Foods

    Article Title: Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

    doi: 10.3390/foods10010018

    Figure Lengend Snippet: The values of coefficient for correlation between viability (metabolic activity) of C6 or HROG36 cells and amount of phenolic compounds in the extracts from leaves of quince cultivars ‘Rondo’, ‘Rasa’, and ‘Darius’.

    Article Snippet: The C6 and HROG36 cell lines were purchased from the Cell Lines Service GmbH (Germany).

    Techniques: Activity Assay

    Representative images of HROG36, C6, and A375 cell spheroid cultures on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h from spheroid positioning. Scale bar is 100 μm.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels

    doi: 10.3389/fbioe.2020.00773

    Figure Lengend Snippet: Representative images of HROG36, C6, and A375 cell spheroid cultures on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h from spheroid positioning. Scale bar is 100 μm.

    Article Snippet: HROG36 ( RRID: CVCL_4U49 ), C6 ( RRID:CVCL_0194 ), and A375 ( RRID: CVCL_0132 ) cell lines were purchased from Cell Lines Service GmbH (Germany).

    Techniques:

    Migration of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cell migration was evaluated by measuring the area of cell spreading around the spheroid in 24 h (A) , by counting cells in the area (B) , and by indicating cell number per mm 2 (C) . The experiments were performed in three independent experimental sets, each of three replicates. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP hydrogels, ∧ – with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels

    doi: 10.3389/fbioe.2020.00773

    Figure Lengend Snippet: Migration of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cell migration was evaluated by measuring the area of cell spreading around the spheroid in 24 h (A) , by counting cells in the area (B) , and by indicating cell number per mm 2 (C) . The experiments were performed in three independent experimental sets, each of three replicates. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP hydrogels, ∧ – with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.

    Article Snippet: HROG36 ( RRID: CVCL_4U49 ), C6 ( RRID:CVCL_0194 ), and A375 ( RRID: CVCL_0132 ) cell lines were purchased from Cell Lines Service GmbH (Germany).

    Techniques: Migration

    Metabolic activity of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h (A) and 48 h (B) . The metabolic activity of the cells corresponding to the proliferation intensity was assessed by Presto Blue TM assay in three independent experimental sets, each of three replicates. RFU, relative fluorescence units. *Indicates a statistically significant difference compared with the samples of the same cell type grown on CLP-only containing hydrogels, ∧ -with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels

    doi: 10.3389/fbioe.2020.00773

    Figure Lengend Snippet: Metabolic activity of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels after 24 h (A) and 48 h (B) . The metabolic activity of the cells corresponding to the proliferation intensity was assessed by Presto Blue TM assay in three independent experimental sets, each of three replicates. RFU, relative fluorescence units. *Indicates a statistically significant difference compared with the samples of the same cell type grown on CLP-only containing hydrogels, ∧ -with samples on PEG-CLP-RGD hydrogels, ANOVA with post hoc LSD test, p < 0.05.

    Article Snippet: HROG36 ( RRID: CVCL_4U49 ), C6 ( RRID:CVCL_0194 ), and A375 ( RRID: CVCL_0132 ) cell lines were purchased from Cell Lines Service GmbH (Germany).

    Techniques: Activity Assay, Fluorescence

    Representative images of focal adhesions formed by HROG36 (A) , C6 (B) , and A375 (C) cells on CLP, CLP-RGD, and CLP-IKVAV hydrogels. The cells were immunostained for actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Scale bar is 10 μm.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels

    doi: 10.3389/fbioe.2020.00773

    Figure Lengend Snippet: Representative images of focal adhesions formed by HROG36 (A) , C6 (B) , and A375 (C) cells on CLP, CLP-RGD, and CLP-IKVAV hydrogels. The cells were immunostained for actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Scale bar is 10 μm.

    Article Snippet: HROG36 ( RRID: CVCL_4U49 ), C6 ( RRID:CVCL_0194 ), and A375 ( RRID: CVCL_0132 ) cell lines were purchased from Cell Lines Service GmbH (Germany).

    Techniques:

    Size distribution of individual focal contacts (A) , projected cell area, cell shape index, and number of focal contacts (B) of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cells were immunostained for F-actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Size distribution of focal contacts of three cells on each hydrogel was plotted together with approximate distribution curve. Projected cell area and perimeter for cell shape index calculation were measured by ImageJ software. Focal adhesion number per cell was calculated according to the number of vinculin-actin colocalization points. The data are presented as AVG ± SDEV of 5–7 cells from three of each hydrogel sample. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP, ∧ – compared with samples on PEG-CLP-RGD. ANOVA with post hoc LSD test, p < 0.05.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels

    doi: 10.3389/fbioe.2020.00773

    Figure Lengend Snippet: Size distribution of individual focal contacts (A) , projected cell area, cell shape index, and number of focal contacts (B) of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cells were immunostained for F-actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Size distribution of focal contacts of three cells on each hydrogel was plotted together with approximate distribution curve. Projected cell area and perimeter for cell shape index calculation were measured by ImageJ software. Focal adhesion number per cell was calculated according to the number of vinculin-actin colocalization points. The data are presented as AVG ± SDEV of 5–7 cells from three of each hydrogel sample. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP, ∧ – compared with samples on PEG-CLP-RGD. ANOVA with post hoc LSD test, p < 0.05.

    Article Snippet: HROG36 ( RRID: CVCL_4U49 ), C6 ( RRID:CVCL_0194 ), and A375 ( RRID: CVCL_0132 ) cell lines were purchased from Cell Lines Service GmbH (Germany).

    Techniques: Software

    Correlations between cell adhesion/morphology parameters and cell migration/proliferation.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Investigation of Cancer Cell Migration and Proliferation on Synthetic Extracellular Matrix Peptide Hydrogels

    doi: 10.3389/fbioe.2020.00773

    Figure Lengend Snippet: Correlations between cell adhesion/morphology parameters and cell migration/proliferation.

    Article Snippet: HROG36 ( RRID: CVCL_4U49 ), C6 ( RRID:CVCL_0194 ), and A375 ( RRID: CVCL_0132 ) cell lines were purchased from Cell Lines Service GmbH (Germany).

    Techniques: Migration