interferon luciferase activity  (Biotium)


Bioz Verified Symbol Biotium is a verified supplier
Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium interferon luciferase activity
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Interferon Luciferase Activity, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/interferon luciferase activity/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    interferon luciferase activity - by Bioz Stars, 2024-05
    94/100 stars

    Images

    1) Product Images from "STING protein-based in situ vaccine synergizes CD4 + T, CD8 + T and NK cells for tumor eradication"

    Article Title: STING protein-based in situ vaccine synergizes CD4 + T, CD8 + T and NK cells for tumor eradication

    Journal: Advanced healthcare materials

    doi: 10.1002/adhm.202300688

    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Figure Legend Snippet: (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.

    Techniques Used: Luciferase, Mutagenesis, Activity Assay, Ex Vivo, Co-Culture Assay, Flow Cytometry, Labeling

    interferon luciferase activity  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium interferon luciferase activity
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Interferon Luciferase Activity, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/interferon luciferase activity/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    interferon luciferase activity - by Bioz Stars, 2024-05
    94/100 stars

    Images

    1) Product Images from "STING protein-based in situ vaccine synergizes CD4 + T, CD8 + T and NK cells for tumor eradication"

    Article Title: STING protein-based in situ vaccine synergizes CD4 + T, CD8 + T and NK cells for tumor eradication

    Journal: Advanced healthcare materials

    doi: 10.1002/adhm.202300688

    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Figure Legend Snippet: (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.

    Techniques Used: Luciferase, Mutagenesis, Activity Assay, Ex Vivo, Co-Culture Assay, Flow Cytometry, Labeling

    interferon luciferase activity  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium interferon luciferase activity
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Interferon Luciferase Activity, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/interferon luciferase activity/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    interferon luciferase activity - by Bioz Stars, 2024-05
    94/100 stars

    Images

    1) Product Images from "STING protein-based in situ vaccine synergizes CD4 + T, CD8 + T and NK cells for tumor eradication"

    Article Title: STING protein-based in situ vaccine synergizes CD4 + T, CD8 + T and NK cells for tumor eradication

    Journal: Advanced healthcare materials

    doi: 10.1002/adhm.202300688

    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Figure Legend Snippet: (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.

    Techniques Used: Luciferase, Mutagenesis, Activity Assay, Ex Vivo, Co-Culture Assay, Flow Cytometry, Labeling

    firefly luciferase assay kit  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium firefly luciferase assay kit
    Firefly Luciferase Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firefly luciferase assay kit/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firefly luciferase assay kit - by Bioz Stars, 2024-05
    94/100 stars

    Images

    firefly luciferase assay kit  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium firefly luciferase assay kit
    Differential activity of NICD heterodimers on various promoters. 293T cells were co-transfected with <t>luciferase</t> reporters containing either the Hes1 (top), Hes4 (middle), or Hes5 (bottom) promoters and either WT NICDs, N2ICD-KK, N4ICD-EE, or a combination of KK and EE NICDs. Hes1 data represents the average +/− SE of five experiments while Hes4 and Hes5 data represents the average +/− SE of four experiments. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.
    Firefly Luciferase Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firefly luciferase assay kit/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firefly luciferase assay kit - by Bioz Stars, 2024-05
    94/100 stars

    Images

    1) Product Images from "Notch intracellular domains form transcriptionally active heterodimeric complexes on sequence-paired sites"

    Article Title: Notch intracellular domains form transcriptionally active heterodimeric complexes on sequence-paired sites

    Journal: Scientific Reports

    doi: 10.1038/s41598-023-50763-4

    Differential activity of NICD heterodimers on various promoters. 293T cells were co-transfected with luciferase reporters containing either the Hes1 (top), Hes4 (middle), or Hes5 (bottom) promoters and either WT NICDs, N2ICD-KK, N4ICD-EE, or a combination of KK and EE NICDs. Hes1 data represents the average +/− SE of five experiments while Hes4 and Hes5 data represents the average +/− SE of four experiments. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.
    Figure Legend Snippet: Differential activity of NICD heterodimers on various promoters. 293T cells were co-transfected with luciferase reporters containing either the Hes1 (top), Hes4 (middle), or Hes5 (bottom) promoters and either WT NICDs, N2ICD-KK, N4ICD-EE, or a combination of KK and EE NICDs. Hes1 data represents the average +/− SE of five experiments while Hes4 and Hes5 data represents the average +/− SE of four experiments. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.

    Techniques Used: Activity Assay, Transfection, Luciferase, Two Tailed Test

    Ankyrin domains are not required for NICD co-immunoprecipitation. ( A ) 293T cells were co-transfected with Hes5-promoter luciferase reporter and wild-type (WT) or corresponding ankyrin domain mutant (Mut) NICDs. Reporter activation is compared to its basal activity in cells, which received an equivalent amount of empty overexpression vector (EV). Shown are the average + /− SE of six experiments. Transfection efficiency was normalized by co-transfection with CMV-β-gal reporter plasmids and measuring β-gal activity. Statistical significance was determined through a student’s two-tailed t test, where *** is p < 0.001, and * is p < 0.05. ( B ) 293T cells were transfected with pairs of cDNAs encoding FLAG- and MYC-tagged WT NICD domains or mutant FLAG- and MYC-tagged mutant ankyrin NICD domains. Anti-FLAG antibodies were used to isolate FLAG-tagged proteins and western blotting with anti-MYC antibodies was used to detect interacting NICD domains. Shown is a representative image of a single experiment that was performed four independent times. To account for dimerization intensities, the figure includes a light and dark exposure of the co-immunoprecipitated MYC-partners. ( C ) 293T cells were transfected with HA-tagged N4ICD and various FLAG-tagged N1ICD constructs including the full-length (FL) N1ICD, N1ICD lacking the C-terminus (RAM/Ank), and the isolated N1ICD ankyrin domain (Ank). Anti-FLAG antibodies were used to detect interacting HA-tagged molecules. Shown is a representative image of a single experiment that was performed three times. ( D ) 293T cells were transfected with full-length HA-tagged N4ICD and either full-length or ankyrin deletion mutant (ΔAnk) FLAG-tagged N4ICD. Co-IP and western analysis were performed as above. In all panels, Input represents 10% of whole cell lysate from each sample as a control for protein expression.
    Figure Legend Snippet: Ankyrin domains are not required for NICD co-immunoprecipitation. ( A ) 293T cells were co-transfected with Hes5-promoter luciferase reporter and wild-type (WT) or corresponding ankyrin domain mutant (Mut) NICDs. Reporter activation is compared to its basal activity in cells, which received an equivalent amount of empty overexpression vector (EV). Shown are the average + /− SE of six experiments. Transfection efficiency was normalized by co-transfection with CMV-β-gal reporter plasmids and measuring β-gal activity. Statistical significance was determined through a student’s two-tailed t test, where *** is p < 0.001, and * is p < 0.05. ( B ) 293T cells were transfected with pairs of cDNAs encoding FLAG- and MYC-tagged WT NICD domains or mutant FLAG- and MYC-tagged mutant ankyrin NICD domains. Anti-FLAG antibodies were used to isolate FLAG-tagged proteins and western blotting with anti-MYC antibodies was used to detect interacting NICD domains. Shown is a representative image of a single experiment that was performed four independent times. To account for dimerization intensities, the figure includes a light and dark exposure of the co-immunoprecipitated MYC-partners. ( C ) 293T cells were transfected with HA-tagged N4ICD and various FLAG-tagged N1ICD constructs including the full-length (FL) N1ICD, N1ICD lacking the C-terminus (RAM/Ank), and the isolated N1ICD ankyrin domain (Ank). Anti-FLAG antibodies were used to detect interacting HA-tagged molecules. Shown is a representative image of a single experiment that was performed three times. ( D ) 293T cells were transfected with full-length HA-tagged N4ICD and either full-length or ankyrin deletion mutant (ΔAnk) FLAG-tagged N4ICD. Co-IP and western analysis were performed as above. In all panels, Input represents 10% of whole cell lysate from each sample as a control for protein expression.

    Techniques Used: Immunoprecipitation, Transfection, Luciferase, Mutagenesis, Activation Assay, Activity Assay, Over Expression, Plasmid Preparation, Cotransfection, Two Tailed Test, Western Blot, Construct, Isolation, Co-Immunoprecipitation Assay, Expressing

    Luciferase assay activity of NICD dimer mutations. 293T cells were co-transfected with a SPS-Core 16 bp promoter luciferase reporter and wild-type (WT) or various NICD mutants. Reporter activation is compared to its basal activity in cells. Shown are the average +/− SE of three experiments. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.
    Figure Legend Snippet: Luciferase assay activity of NICD dimer mutations. 293T cells were co-transfected with a SPS-Core 16 bp promoter luciferase reporter and wild-type (WT) or various NICD mutants. Reporter activation is compared to its basal activity in cells. Shown are the average +/− SE of three experiments. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.

    Techniques Used: Luciferase, Activity Assay, Transfection, Activation Assay, Two Tailed Test

    NICD heterodimers are transcriptionally active. ( A ) 293T cells were co-transfected with a SPS-Core 16 bp promoter luciferase reporter and either WT NICDs or N2ICD-KK, N2ICD-EE, N4ICD-EE or a combination of EE and KK mutants. Shown are the average +/− SE of six experiments. ( B ) Side-by-side comparison of results from 16 and 21 base pair promoters. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.
    Figure Legend Snippet: NICD heterodimers are transcriptionally active. ( A ) 293T cells were co-transfected with a SPS-Core 16 bp promoter luciferase reporter and either WT NICDs or N2ICD-KK, N2ICD-EE, N4ICD-EE or a combination of EE and KK mutants. Shown are the average +/− SE of six experiments. ( B ) Side-by-side comparison of results from 16 and 21 base pair promoters. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.

    Techniques Used: Transfection, Luciferase, Comparison, Two Tailed Test

    firefly luciferase assay kit 2 0  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium firefly luciferase assay kit 2 0
    Firefly Luciferase Assay Kit 2 0, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firefly luciferase assay kit 2 0/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firefly luciferase assay kit 2 0 - by Bioz Stars, 2024-05
    94/100 stars

    Images

    luciferin reagent  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium luciferin reagent
    Luciferin Reagent, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferin reagent/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luciferin reagent - by Bioz Stars, 2024-05
    94/100 stars

    Images

    luciferin reagent  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium luciferin reagent
    Luciferin Reagent, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferin reagent/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luciferin reagent - by Bioz Stars, 2024-05
    94/100 stars

    Images

    firefly luciferase assay kit  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium firefly luciferase assay kit
    Firefly Luciferase Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firefly luciferase assay kit/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firefly luciferase assay kit - by Bioz Stars, 2024-05
    94/100 stars

    Images

    luciferase assay kit  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium luciferase assay kit
    Luciferase Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase assay kit/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luciferase assay kit - by Bioz Stars, 2024-05
    94/100 stars

    Images

    interferon luciferase activity  (Biotium)


    Bioz Verified Symbol Biotium is a verified supplier
    Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Biotium interferon luciferase activity
    Interferon Luciferase Activity, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/interferon luciferase activity/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    interferon luciferase activity - by Bioz Stars, 2024-05
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Biotium interferon luciferase activity
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Interferon Luciferase Activity, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/interferon luciferase activity/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    interferon luciferase activity - by Bioz Stars, 2024-05
    94/100 stars
      Buy from Supplier

    94
    Biotium firefly luciferase assay kit
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Firefly Luciferase Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firefly luciferase assay kit/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firefly luciferase assay kit - by Bioz Stars, 2024-05
    94/100 stars
      Buy from Supplier

    94
    Biotium firefly luciferase assay kit 2 0
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Firefly Luciferase Assay Kit 2 0, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firefly luciferase assay kit 2 0/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firefly luciferase assay kit 2 0 - by Bioz Stars, 2024-05
    94/100 stars
      Buy from Supplier

    94
    Biotium luciferin reagent
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Luciferin Reagent, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferin reagent/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luciferin reagent - by Bioz Stars, 2024-05
    94/100 stars
      Buy from Supplier

    94
    Biotium luciferase assay kit
    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T <t>interferon-luciferase</t> reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.
    Luciferase Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase assay kit/product/Biotium
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luciferase assay kit - by Bioz Stars, 2024-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.

    Journal: Advanced healthcare materials

    Article Title: STING protein-based in situ vaccine synergizes CD4 + T, CD8 + T and NK cells for tumor eradication

    doi: 10.1002/adhm.202300688

    Figure Lengend Snippet: (A) Protein homology-modeling structural prediction of dimerized anti-PDL1-STINGΔTM protein complexed with a cGAMP molecule with SWISS modeling. (B) HEK293T interferon-luciferase reporter cells (N = 3) treated with different ICB-ΔTM-cGAMP complexes, along with S365AΔTM mutant controls. Luciferase activity was determined 24 hours post treatment. (C) Ex vivo assay of OT1 CD8+ T cells’ cytotoxicity against SIINFEKL-MC38 cancer cells, ICB-ΔTM-cGAMP complexes along with equal molar amount of anti-PDL1 antibody were added during the co-culture of CD8+ T cells and cancer cells, the cytotoxicity was measured with flow cytometry with representative plot shown in (D). (E) Representative IVIS images of biodistribution (F) of Cy5-labeled ICB-ΔTM-cGAMP complexes 24 hours post intratumoral injections (tdLN for tumor draining lymph node and cLN for contralateral lymph node). Data were analyzed with one-way ANOVA.

    Article Snippet: 24 hours post treatment, cells were lysed for firefly luciferase assay (Biotium, Cat#: 30075–2) to quantify the interferon-luciferase activity.

    Techniques: Luciferase, Mutagenesis, Activity Assay, Ex Vivo, Co-Culture Assay, Flow Cytometry, Labeling