human monocyte thp 1 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human monocyte thp 1 cells
    Human Monocyte Thp 1 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human monocyte thp 1 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human monocyte thp 1 cells
    Human Monocyte Thp 1 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thp 1 cell culture  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH thp 1 cell culture
    Thp 1 Cell Culture, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thp 1 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH thp 1 cells
    In vitro characterization of PMA-treated and activated <t>THP-1</t> cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.
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    1) Product Images from "Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function"

    Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

    Journal: Biomedicines

    doi: 10.3390/biomedicines10071524

    In vitro characterization of PMA-treated and activated THP-1 cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.
    Figure Legend Snippet: In vitro characterization of PMA-treated and activated THP-1 cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.

    Techniques Used: In Vitro, Expressing, Cell Culture, Fluorescence

    Effect of resveratrol (Res) and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) on THP-1 cells activated with LPS/INF-γ (panels A – E ) and IL-4/IL-13 (panels F – I ). Levels of CD11b ( A , F ), CD11c ( B , G ), CD14 ( C ), CD206 ( D ), CD209 ( H ), CD274 ( I ) and HLA-DR ( E ) expression were analysed by cytofluorometry in THP-1 cells activated for 20 h in the absence of substances (black), with Res (red) and with ω-3 PUFAs (green). Substances induced MFI intensity differences of ≈2-fold (e.g., ω-3 PUFAs in CD11b ( A )) to >10-fold (e.g., Res in CD209 [H]). MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.
    Figure Legend Snippet: Effect of resveratrol (Res) and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) on THP-1 cells activated with LPS/INF-γ (panels A – E ) and IL-4/IL-13 (panels F – I ). Levels of CD11b ( A , F ), CD11c ( B , G ), CD14 ( C ), CD206 ( D ), CD209 ( H ), CD274 ( I ) and HLA-DR ( E ) expression were analysed by cytofluorometry in THP-1 cells activated for 20 h in the absence of substances (black), with Res (red) and with ω-3 PUFAs (green). Substances induced MFI intensity differences of ≈2-fold (e.g., ω-3 PUFAs in CD11b ( A )) to >10-fold (e.g., Res in CD209 [H]). MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.

    Techniques Used: Expressing

    Secretion of chemokines and cytokines by  THP-1 cells,  which were polarized by GM-CSF (to M1) and M-CSF (to M2), respectively, for 6 days and activated with LPS/INF-γ or IL-4/IL-13 for 24 h. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ vs. IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).
    Figure Legend Snippet: Secretion of chemokines and cytokines by THP-1 cells, which were polarized by GM-CSF (to M1) and M-CSF (to M2), respectively, for 6 days and activated with LPS/INF-γ or IL-4/IL-13 for 24 h. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ vs. IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).

    Techniques Used: Standard Deviation, Activation Assay

    Effect of resveratrol and ω-3 PUFAs on chemokines and cytokine secretion by PMA-treated  THP-1 cells,  that were cultured for 48 h and, where indicated, activated with LPS/INF-γ or IL-4/IL-13 for 24 h before harvesting. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ induced secretion versus IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).
    Figure Legend Snippet: Effect of resveratrol and ω-3 PUFAs on chemokines and cytokine secretion by PMA-treated THP-1 cells, that were cultured for 48 h and, where indicated, activated with LPS/INF-γ or IL-4/IL-13 for 24 h before harvesting. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ induced secretion versus IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).

    Techniques Used: Cell Culture, Standard Deviation, Activation Assay

    Secretion of cytokines and chemokines by THP-1 cells stimulated with LPS/IFN-γ or IL-4/IL-13. Cells were cultured for 20 h and culture supernatants analysed for interleukins/cytokines and chemokines. Black squares on the y-axis indicate the level of metabolite secreted by unstimulated cells. ( A ) TNF-α, ( B ) IL-1β, ( C ) IL-6, ( D ) CXCL10/IP-10, ( E ) CCL13/MCP-4, ( F ) CCL18/PARC, ( G ) CCL20/MIP-3α. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. * p < 0.05, ** p < 0.01 (compared to LPS/INF-γ or IL-4/IL-13-activated cells).
    Figure Legend Snippet: Secretion of cytokines and chemokines by THP-1 cells stimulated with LPS/IFN-γ or IL-4/IL-13. Cells were cultured for 20 h and culture supernatants analysed for interleukins/cytokines and chemokines. Black squares on the y-axis indicate the level of metabolite secreted by unstimulated cells. ( A ) TNF-α, ( B ) IL-1β, ( C ) IL-6, ( D ) CXCL10/IP-10, ( E ) CCL13/MCP-4, ( F ) CCL18/PARC, ( G ) CCL20/MIP-3α. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. * p < 0.05, ** p < 0.01 (compared to LPS/INF-γ or IL-4/IL-13-activated cells).

    Techniques Used: Cell Culture, Standard Deviation

    thp 1  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH thp 1
    Thp 1, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thp 1 cell culture thp 1 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH thp 1 cell culture thp 1 cells
    Thp 1 Cell Culture Thp 1 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thp 1 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH thp 1 cells
    ELISA results showing IL-1β released after LPS stimulation of PMA- or VitD3- differentiated <t>THP-1</t> cells. IL-1β was measured for cells that were treated with 0 and 100 μg/mL CHOS for 24 h before challenging with 100 ng/mL LPS for 7 h. The IL-1β concentration for cells that were not challenged with LPS (control) is shown for comparison. The results are expressed as the mean ± SD of duplicates from three independent experiments (n = 6); a indicates significant difference at ( P < 0.001).
    Thp 1 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti-inflammatory activity of soluble chito-oligosaccharides (CHOS) on VitD3-induced human THP-1 monocytes"

    Article Title: Anti-inflammatory activity of soluble chito-oligosaccharides (CHOS) on VitD3-induced human THP-1 monocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0246381

    ELISA results showing IL-1β released after LPS stimulation of PMA- or VitD3- differentiated THP-1 cells. IL-1β was measured for cells that were treated with 0 and 100 μg/mL CHOS for 24 h before challenging with 100 ng/mL LPS for 7 h. The IL-1β concentration for cells that were not challenged with LPS (control) is shown for comparison. The results are expressed as the mean ± SD of duplicates from three independent experiments (n = 6); a indicates significant difference at ( P < 0.001).
    Figure Legend Snippet: ELISA results showing IL-1β released after LPS stimulation of PMA- or VitD3- differentiated THP-1 cells. IL-1β was measured for cells that were treated with 0 and 100 μg/mL CHOS for 24 h before challenging with 100 ng/mL LPS for 7 h. The IL-1β concentration for cells that were not challenged with LPS (control) is shown for comparison. The results are expressed as the mean ± SD of duplicates from three independent experiments (n = 6); a indicates significant difference at ( P < 0.001).

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay

    Panels ( A ) and ( B ) show representative experiments of flow cytometry dot plots for PMA- and VitD3- differentiated cells, respectively. The bar graphs show analyses of CD14 expression and PI staining for PMA- ( C ) and VitD3-differentiated ( D ) THP-1 cells, measured by flow-cytometry at different time points, namely 0, 48, 72 and 96 h. The percentages of CD14- and PI- stained cells in comparison to the un-stimulated THP-1 cells (time 0) are shown. The bars show the mean ± SD of three independent duplicated experiments (n = 6); a = ( P < 0.001) and b = ( P < 0.01) indicate significant differences, relative to the control (0 time). Note that for PMA treated cells, the medium containing PMA was removed after 72 h to fresh media in order to get rid of PMA.
    Figure Legend Snippet: Panels ( A ) and ( B ) show representative experiments of flow cytometry dot plots for PMA- and VitD3- differentiated cells, respectively. The bar graphs show analyses of CD14 expression and PI staining for PMA- ( C ) and VitD3-differentiated ( D ) THP-1 cells, measured by flow-cytometry at different time points, namely 0, 48, 72 and 96 h. The percentages of CD14- and PI- stained cells in comparison to the un-stimulated THP-1 cells (time 0) are shown. The bars show the mean ± SD of three independent duplicated experiments (n = 6); a = ( P < 0.001) and b = ( P < 0.01) indicate significant differences, relative to the control (0 time). Note that for PMA treated cells, the medium containing PMA was removed after 72 h to fresh media in order to get rid of PMA.

    Techniques Used: Flow Cytometry, Expressing, Staining

    The MTT assay was used to determine cell viability after VitD3-differentiated THP-1 cells had been treated with various concentrations of CHOS for 24 h. Relative cell viability was calculated based on untreated cells (0 μg/ml, 100%). The effect of LPS on the viability of VitD3-differentiated THP-1 cells was also determined, as shown by the grey bar. The data shown are mean±SD from three independent triplicated experiments (n = 9). A Kruskal-Wallis test indicated no significant differences relative to cells treated with 0 μg/mL CHOS.
    Figure Legend Snippet: The MTT assay was used to determine cell viability after VitD3-differentiated THP-1 cells had been treated with various concentrations of CHOS for 24 h. Relative cell viability was calculated based on untreated cells (0 μg/ml, 100%). The effect of LPS on the viability of VitD3-differentiated THP-1 cells was also determined, as shown by the grey bar. The data shown are mean±SD from three independent triplicated experiments (n = 9). A Kruskal-Wallis test indicated no significant differences relative to cells treated with 0 μg/mL CHOS.

    Techniques Used: MTT Assay

    The effects of CHOS, at final concentrations ranging from 0 to 200 μg/mL, on release of IL-1β ( A ), IL-6 ( B ), and TNF-α ( C ) by LPS-stimulated, VitD3-differentiated THP-1 cells were determined by ELISA. Dexamethasone (0.5 μM), a standard anti-inflammatory drug, was used as a positive control. ( D ) An extra washing step was done after CHOS treatment before LPS challenging. The shown data are from three independent duplicated experiments (n = 6) except Fig 6D, which reflects one triplicated experiment (n = 3). Significance levels: a = P < 0.001, b = P < 0.01, c = P < 0.05 (compared with 0 μg/mL CHOS). See for an enlargement of data from the experiments without LPS stimulation (-LPS).
    Figure Legend Snippet: The effects of CHOS, at final concentrations ranging from 0 to 200 μg/mL, on release of IL-1β ( A ), IL-6 ( B ), and TNF-α ( C ) by LPS-stimulated, VitD3-differentiated THP-1 cells were determined by ELISA. Dexamethasone (0.5 μM), a standard anti-inflammatory drug, was used as a positive control. ( D ) An extra washing step was done after CHOS treatment before LPS challenging. The shown data are from three independent duplicated experiments (n = 6) except Fig 6D, which reflects one triplicated experiment (n = 3). Significance levels: a = P < 0.001, b = P < 0.01, c = P < 0.05 (compared with 0 μg/mL CHOS). See for an enlargement of data from the experiments without LPS stimulation (-LPS).

    Techniques Used: Enzyme-linked Immunosorbent Assay, Positive Control

    Both non-differentiated and VitD3 differentiated THP-1 cells were treated with 100 μg/mL of CHOS and 100 ng/mL of LPS. Flow cytometry was used to determine CD14 expression and PI staining. Panels ( A) and ( B) show flow cytometry dot plots for representative experiments. The bar chart ( C ) is generated from the average of data from the upper-left quadrant of three independent experiments. The data represent three independent duplicated experiments (n = 6). NS means non-significant.
    Figure Legend Snippet: Both non-differentiated and VitD3 differentiated THP-1 cells were treated with 100 μg/mL of CHOS and 100 ng/mL of LPS. Flow cytometry was used to determine CD14 expression and PI staining. Panels ( A) and ( B) show flow cytometry dot plots for representative experiments. The bar chart ( C ) is generated from the average of data from the upper-left quadrant of three independent experiments. The data represent three independent duplicated experiments (n = 6). NS means non-significant.

    Techniques Used: Flow Cytometry, Expressing, Staining, Generated

    thp 1 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH thp 1 cells
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    CLS Cell Lines Service GmbH thp 1 cells
    Cytotoxic activity of the nigritoxin on different cell lines. a Two insect (Sf9 and S2) and four human (HeLa, hTERT RPE-1, Jurkat, and <t>THP-1)</t> cell lines were incubated with nigritoxin (1.2 μM) for 0, 6, 12, and 24 h (white, light gray, dark gray, and black bars, respectively). The cytotoxicity expressed in percentage ( y -axis) was monitored using Alamar Blue assay. The experiment was performed twice in triplicate and data are presented as mean ± S.D. A star indicates significant difference between treatment (ANOVA, p < 0.05) b Sf9 and S2 cells (black and gray bars, respectively) were either mock-treated or incubated with 1.2 μM nigritoxin for 12 h. Caspase activity was determined using Ac-DEVD-MCA as a substrate. The experiment was performed in triplicate and data are presented as mean ± S.D. Means with the same letter are not significantly different from each other (Kruskal–Wallis, p < 0.05). c Insect and human cell lines were incubated with nigritoxin (1.2 μM) for 6 h, then washed, fixed, and reacted with a specific anti-nigritoxin polyclonal antibody and Alexa Fluor-488-conjugated secondary antibody (green). Nuclei were stained with DAPI. Left image, mock-treated cells; right image, nigritoxin-treated cells. d Primary hemocytes from shrimp Litopenaeus vannamei were treated and analyzed as described in c . Scale bars: 20 μM
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    1) Product Images from "Nigritoxin is a bacterial toxin for crustaceans and insects"

    Article Title: Nigritoxin is a bacterial toxin for crustaceans and insects

    Journal: Nature Communications

    doi: 10.1038/s41467-017-01445-z

    Cytotoxic activity of the nigritoxin on different cell lines. a Two insect (Sf9 and S2) and four human (HeLa, hTERT RPE-1, Jurkat, and THP-1) cell lines were incubated with nigritoxin (1.2 μM) for 0, 6, 12, and 24 h (white, light gray, dark gray, and black bars, respectively). The cytotoxicity expressed in percentage ( y -axis) was monitored using Alamar Blue assay. The experiment was performed twice in triplicate and data are presented as mean ± S.D. A star indicates significant difference between treatment (ANOVA, p < 0.05) b Sf9 and S2 cells (black and gray bars, respectively) were either mock-treated or incubated with 1.2 μM nigritoxin for 12 h. Caspase activity was determined using Ac-DEVD-MCA as a substrate. The experiment was performed in triplicate and data are presented as mean ± S.D. Means with the same letter are not significantly different from each other (Kruskal–Wallis, p < 0.05). c Insect and human cell lines were incubated with nigritoxin (1.2 μM) for 6 h, then washed, fixed, and reacted with a specific anti-nigritoxin polyclonal antibody and Alexa Fluor-488-conjugated secondary antibody (green). Nuclei were stained with DAPI. Left image, mock-treated cells; right image, nigritoxin-treated cells. d Primary hemocytes from shrimp Litopenaeus vannamei were treated and analyzed as described in c . Scale bars: 20 μM
    Figure Legend Snippet: Cytotoxic activity of the nigritoxin on different cell lines. a Two insect (Sf9 and S2) and four human (HeLa, hTERT RPE-1, Jurkat, and THP-1) cell lines were incubated with nigritoxin (1.2 μM) for 0, 6, 12, and 24 h (white, light gray, dark gray, and black bars, respectively). The cytotoxicity expressed in percentage ( y -axis) was monitored using Alamar Blue assay. The experiment was performed twice in triplicate and data are presented as mean ± S.D. A star indicates significant difference between treatment (ANOVA, p < 0.05) b Sf9 and S2 cells (black and gray bars, respectively) were either mock-treated or incubated with 1.2 μM nigritoxin for 12 h. Caspase activity was determined using Ac-DEVD-MCA as a substrate. The experiment was performed in triplicate and data are presented as mean ± S.D. Means with the same letter are not significantly different from each other (Kruskal–Wallis, p < 0.05). c Insect and human cell lines were incubated with nigritoxin (1.2 μM) for 6 h, then washed, fixed, and reacted with a specific anti-nigritoxin polyclonal antibody and Alexa Fluor-488-conjugated secondary antibody (green). Nuclei were stained with DAPI. Left image, mock-treated cells; right image, nigritoxin-treated cells. d Primary hemocytes from shrimp Litopenaeus vannamei were treated and analyzed as described in c . Scale bars: 20 μM

    Techniques Used: Activity Assay, Incubation, Alamar Blue Assay, Staining

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    CLS Cell Lines Service GmbH thp 1 cells
    Effect of RES on gene expression in activated <t>THP-1.</t> PMA-treated THP-1 cells were cultured in the presence of indicated concentrations of RES and activated with LPS for 4 h. RT-PCR was performed and the gene expression levels were indicated as mean fold changes (± errors) (versus unstimulated cell) (see also reference ). * p < 0.05, ** p < 0.01, *** p < 0.001 (versus LPS-stimulated cells)
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    1) Product Images from "Resveratrol distinctively modulates the inflammatory profiles of immune and endothelial cells"

    Article Title: Resveratrol distinctively modulates the inflammatory profiles of immune and endothelial cells

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/s12906-017-1823-z

    Effect of RES on gene expression in activated THP-1. PMA-treated THP-1 cells were cultured in the presence of indicated concentrations of RES and activated with LPS for 4 h. RT-PCR was performed and the gene expression levels were indicated as mean fold changes (± errors) (versus unstimulated cell) (see also reference ). * p < 0.05, ** p < 0.01, *** p < 0.001 (versus LPS-stimulated cells)
    Figure Legend Snippet: Effect of RES on gene expression in activated THP-1. PMA-treated THP-1 cells were cultured in the presence of indicated concentrations of RES and activated with LPS for 4 h. RT-PCR was performed and the gene expression levels were indicated as mean fold changes (± errors) (versus unstimulated cell) (see also reference ). * p < 0.05, ** p < 0.01, *** p < 0.001 (versus LPS-stimulated cells)

    Techniques Used: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

    Resveratrol reduced the secretion of cytokines and chemokines in LPS-activated  THP-1 cells
    Figure Legend Snippet: Resveratrol reduced the secretion of cytokines and chemokines in LPS-activated THP-1 cells

    Techniques Used:

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    In vitro characterization of PMA-treated and activated <t>THP-1</t> cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.
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    CLS Cell Lines Service GmbH thp 1
    In vitro characterization of PMA-treated and activated <t>THP-1</t> cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.
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    CLS Cell Lines Service GmbH thp 1 cell culture thp 1 cells
    In vitro characterization of PMA-treated and activated <t>THP-1</t> cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.
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    In vitro characterization of PMA-treated and activated THP-1 cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.

    Journal: Biomedicines

    Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

    doi: 10.3390/biomedicines10071524

    Figure Lengend Snippet: In vitro characterization of PMA-treated and activated THP-1 cells. Levels of ( A ) CD11b, ( B ) CD11c, ( C ) CD14, ( D ) CD80, ( E ) CD209, ( F ) CD274 and ( G ) HLA-DR expression were analysed by cytofluorometry in THP-1 cells, which had been cultured for 20 h in medium only (black), or activated with LPS/INF-γ (red) and IL-4/IL-13 (blue). Results are representative of two different experimental series, each carried out in duplicates. Mean fluorescence intensity (MFI) variations between curves are ≈2- to ≈50-fold due the logarithmic scale on the x-axis. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.

    Article Snippet: THP-1 cells, a human leukaemia monocytic cell line, were from Cell Lines Service (Eppelheim, Germany) and maintained at <2 × 10 5 cells/mL in RPMI 1640 medium supplemented with P/S, 10% FCS and 2 mM L-glutamine.

    Techniques: In Vitro, Expressing, Cell Culture, Fluorescence

    Effect of resveratrol (Res) and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) on THP-1 cells activated with LPS/INF-γ (panels A – E ) and IL-4/IL-13 (panels F – I ). Levels of CD11b ( A , F ), CD11c ( B , G ), CD14 ( C ), CD206 ( D ), CD209 ( H ), CD274 ( I ) and HLA-DR ( E ) expression were analysed by cytofluorometry in THP-1 cells activated for 20 h in the absence of substances (black), with Res (red) and with ω-3 PUFAs (green). Substances induced MFI intensity differences of ≈2-fold (e.g., ω-3 PUFAs in CD11b ( A )) to >10-fold (e.g., Res in CD209 [H]). MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.

    Journal: Biomedicines

    Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

    doi: 10.3390/biomedicines10071524

    Figure Lengend Snippet: Effect of resveratrol (Res) and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) on THP-1 cells activated with LPS/INF-γ (panels A – E ) and IL-4/IL-13 (panels F – I ). Levels of CD11b ( A , F ), CD11c ( B , G ), CD14 ( C ), CD206 ( D ), CD209 ( H ), CD274 ( I ) and HLA-DR ( E ) expression were analysed by cytofluorometry in THP-1 cells activated for 20 h in the absence of substances (black), with Res (red) and with ω-3 PUFAs (green). Substances induced MFI intensity differences of ≈2-fold (e.g., ω-3 PUFAs in CD11b ( A )) to >10-fold (e.g., Res in CD209 [H]). MFI values are indicated for the major peak of the colour-coded cytofluorometric curves.

    Article Snippet: THP-1 cells, a human leukaemia monocytic cell line, were from Cell Lines Service (Eppelheim, Germany) and maintained at <2 × 10 5 cells/mL in RPMI 1640 medium supplemented with P/S, 10% FCS and 2 mM L-glutamine.

    Techniques: Expressing

    Secretion of chemokines and cytokines by  THP-1 cells,  which were polarized by GM-CSF (to M1) and M-CSF (to M2), respectively, for 6 days and activated with LPS/INF-γ or IL-4/IL-13 for 24 h. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ vs. IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).

    Journal: Biomedicines

    Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

    doi: 10.3390/biomedicines10071524

    Figure Lengend Snippet: Secretion of chemokines and cytokines by THP-1 cells, which were polarized by GM-CSF (to M1) and M-CSF (to M2), respectively, for 6 days and activated with LPS/INF-γ or IL-4/IL-13 for 24 h. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ vs. IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).

    Article Snippet: THP-1 cells, a human leukaemia monocytic cell line, were from Cell Lines Service (Eppelheim, Germany) and maintained at <2 × 10 5 cells/mL in RPMI 1640 medium supplemented with P/S, 10% FCS and 2 mM L-glutamine.

    Techniques: Standard Deviation, Activation Assay

    Effect of resveratrol and ω-3 PUFAs on chemokines and cytokine secretion by PMA-treated  THP-1 cells,  that were cultured for 48 h and, where indicated, activated with LPS/INF-γ or IL-4/IL-13 for 24 h before harvesting. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ induced secretion versus IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).

    Journal: Biomedicines

    Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

    doi: 10.3390/biomedicines10071524

    Figure Lengend Snippet: Effect of resveratrol and ω-3 PUFAs on chemokines and cytokine secretion by PMA-treated THP-1 cells, that were cultured for 48 h and, where indicated, activated with LPS/INF-γ or IL-4/IL-13 for 24 h before harvesting. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN-γ induced secretion versus IL-4/IL-13 induced secretion (assuming limit of detection of 1 pg/mL *).

    Article Snippet: THP-1 cells, a human leukaemia monocytic cell line, were from Cell Lines Service (Eppelheim, Germany) and maintained at <2 × 10 5 cells/mL in RPMI 1640 medium supplemented with P/S, 10% FCS and 2 mM L-glutamine.

    Techniques: Cell Culture, Standard Deviation, Activation Assay

    Secretion of cytokines and chemokines by THP-1 cells stimulated with LPS/IFN-γ or IL-4/IL-13. Cells were cultured for 20 h and culture supernatants analysed for interleukins/cytokines and chemokines. Black squares on the y-axis indicate the level of metabolite secreted by unstimulated cells. ( A ) TNF-α, ( B ) IL-1β, ( C ) IL-6, ( D ) CXCL10/IP-10, ( E ) CCL13/MCP-4, ( F ) CCL18/PARC, ( G ) CCL20/MIP-3α. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. * p < 0.05, ** p < 0.01 (compared to LPS/INF-γ or IL-4/IL-13-activated cells).

    Journal: Biomedicines

    Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

    doi: 10.3390/biomedicines10071524

    Figure Lengend Snippet: Secretion of cytokines and chemokines by THP-1 cells stimulated with LPS/IFN-γ or IL-4/IL-13. Cells were cultured for 20 h and culture supernatants analysed for interleukins/cytokines and chemokines. Black squares on the y-axis indicate the level of metabolite secreted by unstimulated cells. ( A ) TNF-α, ( B ) IL-1β, ( C ) IL-6, ( D ) CXCL10/IP-10, ( E ) CCL13/MCP-4, ( F ) CCL18/PARC, ( G ) CCL20/MIP-3α. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. * p < 0.05, ** p < 0.01 (compared to LPS/INF-γ or IL-4/IL-13-activated cells).

    Article Snippet: THP-1 cells, a human leukaemia monocytic cell line, were from Cell Lines Service (Eppelheim, Germany) and maintained at <2 × 10 5 cells/mL in RPMI 1640 medium supplemented with P/S, 10% FCS and 2 mM L-glutamine.

    Techniques: Cell Culture, Standard Deviation