u2os zfn  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH u2os zfn
    U2os Zfn, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    u2os zfn  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH u2os zfn
    U2os Zfn, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    u2os nup107 snap tag cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH u2os nup107 snap tag cells
    Maps of dark current ( a ) and noise ( b ) at 500 ms single frame exposure time for a scientific-grade CMOS (sCMOS) camera cooled to the manufacturer’s calibration setpoint of −10 °C. Simulations of 3D DNA-PAINT via astigmatism-based PSF shaping using this camera reveal a particular pattern in the localization bias close to pixels of high dark current, both laterally ( c ) and axially ( e ) when not applying (s)CMOS-specific fitting that corrects for pixel-wise effects including thermal effects. Explicit application of (s)CMOS specific fitting largely removes the bias for DNA-PAINT ( d , f ) as well as STORM and PALM ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D DNA-PAINT data of the nucleoporin Nup96 in <t>U2OS</t> cells using the same cooled sCMOS camera. The image is rendered as an overlay of the pixel dark current map (red) and the SMLM reconstruction with no camera correction (magenta) and with CMOS correction (green). j , Zoom into boxed region in i . k , l , The structure of a nuclear pore complex (indicated by the boxes in ( m ), ( n )) becomes shifted in the vicinity of a pixel of high dark current, both in axial ( k ) and lateral ( l ) direction when neglecting individual pixel characteristics including thermal effects in the fitting pipeline. m Axial view of the region indicated in ( j ), also shown in Supplementary Video . n Lateral close up of the nuclear pore complex indicated in ( m ). As expected from the simulation, the shift features a high spatial dependence ( m , n ), which even changes its sign (indicated by the red arrows in n ).
    U2os Nup107 Snap Tag Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    1) Product Images from "Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy"

    Article Title: Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy

    Journal: Nature Communications

    doi: 10.1038/s41467-022-30907-2

    Maps of dark current ( a ) and noise ( b ) at 500 ms single frame exposure time for a scientific-grade CMOS (sCMOS) camera cooled to the manufacturer’s calibration setpoint of −10 °C. Simulations of 3D DNA-PAINT via astigmatism-based PSF shaping using this camera reveal a particular pattern in the localization bias close to pixels of high dark current, both laterally ( c ) and axially ( e ) when not applying (s)CMOS-specific fitting that corrects for pixel-wise effects including thermal effects. Explicit application of (s)CMOS specific fitting largely removes the bias for DNA-PAINT ( d , f ) as well as STORM and PALM ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D DNA-PAINT data of the nucleoporin Nup96 in U2OS cells using the same cooled sCMOS camera. The image is rendered as an overlay of the pixel dark current map (red) and the SMLM reconstruction with no camera correction (magenta) and with CMOS correction (green). j , Zoom into boxed region in i . k , l , The structure of a nuclear pore complex (indicated by the boxes in ( m ), ( n )) becomes shifted in the vicinity of a pixel of high dark current, both in axial ( k ) and lateral ( l ) direction when neglecting individual pixel characteristics including thermal effects in the fitting pipeline. m Axial view of the region indicated in ( j ), also shown in Supplementary Video . n Lateral close up of the nuclear pore complex indicated in ( m ). As expected from the simulation, the shift features a high spatial dependence ( m , n ), which even changes its sign (indicated by the red arrows in n ).
    Figure Legend Snippet: Maps of dark current ( a ) and noise ( b ) at 500 ms single frame exposure time for a scientific-grade CMOS (sCMOS) camera cooled to the manufacturer’s calibration setpoint of −10 °C. Simulations of 3D DNA-PAINT via astigmatism-based PSF shaping using this camera reveal a particular pattern in the localization bias close to pixels of high dark current, both laterally ( c ) and axially ( e ) when not applying (s)CMOS-specific fitting that corrects for pixel-wise effects including thermal effects. Explicit application of (s)CMOS specific fitting largely removes the bias for DNA-PAINT ( d , f ) as well as STORM and PALM ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D DNA-PAINT data of the nucleoporin Nup96 in U2OS cells using the same cooled sCMOS camera. The image is rendered as an overlay of the pixel dark current map (red) and the SMLM reconstruction with no camera correction (magenta) and with CMOS correction (green). j , Zoom into boxed region in i . k , l , The structure of a nuclear pore complex (indicated by the boxes in ( m ), ( n )) becomes shifted in the vicinity of a pixel of high dark current, both in axial ( k ) and lateral ( l ) direction when neglecting individual pixel characteristics including thermal effects in the fitting pipeline. m Axial view of the region indicated in ( j ), also shown in Supplementary Video . n Lateral close up of the nuclear pore complex indicated in ( m ). As expected from the simulation, the shift features a high spatial dependence ( m , n ), which even changes its sign (indicated by the red arrows in n ).

    Techniques Used:

    Maps of dark current ( a ) and noise ( b ) at 50 ms single frame exposure time for an uncooled, industry-grade CMOS camera (characteristics shown in Fig. ). Simulations of astigmatism-based 3D PALM without explicit consideration of pixel-wise effects show a similar pattern in the localization bias, but of greater amplitude as compared to the cooled sCMOS camera (compare Fig. ), both laterally ( c ) and axially ( e ). Explicit application of CMOS-specific fitting largely removes the bias for PALM ( d , f ) as well as STORM and DNA-PAINT ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D PALM data of clathrin tagged with mEOS3.2 in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. j , Axial view of region indicated in i . k Experimental 3D STORM data of Nup107-SNAP, labeled with AF 647, in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. l Gallery showing lateral and axial views on individual nuclear pore complexes indicated in k . m The axial view on the region indicated in ( k ) shows two parallel lines from the nucleo- and cytoplasmic rings 57 nm apart. n First frame of experimental raw data from time-lapse TIRF imaging of AP-2 tagged with eGFP in live U373 cells recorded at 1000 ms single frame exposure time. o NCS corrected and ( p ) ACsN corrected frame. The entire time-lapse for ( n – p ) is shown in Supplementary Video . q The noise of a pixel of high dark current is strongly reduced via both approaches after appropriate characterization of the camera including thermal effects. The signal has been offset-corrected by the pixel value of the first frame from the time-lapse.
    Figure Legend Snippet: Maps of dark current ( a ) and noise ( b ) at 50 ms single frame exposure time for an uncooled, industry-grade CMOS camera (characteristics shown in Fig. ). Simulations of astigmatism-based 3D PALM without explicit consideration of pixel-wise effects show a similar pattern in the localization bias, but of greater amplitude as compared to the cooled sCMOS camera (compare Fig. ), both laterally ( c ) and axially ( e ). Explicit application of CMOS-specific fitting largely removes the bias for PALM ( d , f ) as well as STORM and DNA-PAINT ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D PALM data of clathrin tagged with mEOS3.2 in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. j , Axial view of region indicated in i . k Experimental 3D STORM data of Nup107-SNAP, labeled with AF 647, in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. l Gallery showing lateral and axial views on individual nuclear pore complexes indicated in k . m The axial view on the region indicated in ( k ) shows two parallel lines from the nucleo- and cytoplasmic rings 57 nm apart. n First frame of experimental raw data from time-lapse TIRF imaging of AP-2 tagged with eGFP in live U373 cells recorded at 1000 ms single frame exposure time. o NCS corrected and ( p ) ACsN corrected frame. The entire time-lapse for ( n – p ) is shown in Supplementary Video . q The noise of a pixel of high dark current is strongly reduced via both approaches after appropriate characterization of the camera including thermal effects. The signal has been offset-corrected by the pixel value of the first frame from the time-lapse.

    Techniques Used: Labeling, Imaging

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    CLS Cell Lines Service GmbH u2os zfn
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    CLS Cell Lines Service GmbH u2os nup107 snap tag cells
    Maps of dark current ( a ) and noise ( b ) at 500 ms single frame exposure time for a scientific-grade CMOS (sCMOS) camera cooled to the manufacturer’s calibration setpoint of −10 °C. Simulations of 3D DNA-PAINT via astigmatism-based PSF shaping using this camera reveal a particular pattern in the localization bias close to pixels of high dark current, both laterally ( c ) and axially ( e ) when not applying (s)CMOS-specific fitting that corrects for pixel-wise effects including thermal effects. Explicit application of (s)CMOS specific fitting largely removes the bias for DNA-PAINT ( d , f ) as well as STORM and PALM ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D DNA-PAINT data of the nucleoporin Nup96 in <t>U2OS</t> cells using the same cooled sCMOS camera. The image is rendered as an overlay of the pixel dark current map (red) and the SMLM reconstruction with no camera correction (magenta) and with CMOS correction (green). j , Zoom into boxed region in i . k , l , The structure of a nuclear pore complex (indicated by the boxes in ( m ), ( n )) becomes shifted in the vicinity of a pixel of high dark current, both in axial ( k ) and lateral ( l ) direction when neglecting individual pixel characteristics including thermal effects in the fitting pipeline. m Axial view of the region indicated in ( j ), also shown in Supplementary Video . n Lateral close up of the nuclear pore complex indicated in ( m ). As expected from the simulation, the shift features a high spatial dependence ( m , n ), which even changes its sign (indicated by the red arrows in n ).
    U2os Nup107 Snap Tag Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u2os nup107 snap tag cells/product/CLS Cell Lines Service GmbH
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Maps of dark current ( a ) and noise ( b ) at 500 ms single frame exposure time for a scientific-grade CMOS (sCMOS) camera cooled to the manufacturer’s calibration setpoint of −10 °C. Simulations of 3D DNA-PAINT via astigmatism-based PSF shaping using this camera reveal a particular pattern in the localization bias close to pixels of high dark current, both laterally ( c ) and axially ( e ) when not applying (s)CMOS-specific fitting that corrects for pixel-wise effects including thermal effects. Explicit application of (s)CMOS specific fitting largely removes the bias for DNA-PAINT ( d , f ) as well as STORM and PALM ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D DNA-PAINT data of the nucleoporin Nup96 in U2OS cells using the same cooled sCMOS camera. The image is rendered as an overlay of the pixel dark current map (red) and the SMLM reconstruction with no camera correction (magenta) and with CMOS correction (green). j , Zoom into boxed region in i . k , l , The structure of a nuclear pore complex (indicated by the boxes in ( m ), ( n )) becomes shifted in the vicinity of a pixel of high dark current, both in axial ( k ) and lateral ( l ) direction when neglecting individual pixel characteristics including thermal effects in the fitting pipeline. m Axial view of the region indicated in ( j ), also shown in Supplementary Video . n Lateral close up of the nuclear pore complex indicated in ( m ). As expected from the simulation, the shift features a high spatial dependence ( m , n ), which even changes its sign (indicated by the red arrows in n ).

    Journal: Nature Communications

    Article Title: Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy

    doi: 10.1038/s41467-022-30907-2

    Figure Lengend Snippet: Maps of dark current ( a ) and noise ( b ) at 500 ms single frame exposure time for a scientific-grade CMOS (sCMOS) camera cooled to the manufacturer’s calibration setpoint of −10 °C. Simulations of 3D DNA-PAINT via astigmatism-based PSF shaping using this camera reveal a particular pattern in the localization bias close to pixels of high dark current, both laterally ( c ) and axially ( e ) when not applying (s)CMOS-specific fitting that corrects for pixel-wise effects including thermal effects. Explicit application of (s)CMOS specific fitting largely removes the bias for DNA-PAINT ( d , f ) as well as STORM and PALM ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D DNA-PAINT data of the nucleoporin Nup96 in U2OS cells using the same cooled sCMOS camera. The image is rendered as an overlay of the pixel dark current map (red) and the SMLM reconstruction with no camera correction (magenta) and with CMOS correction (green). j , Zoom into boxed region in i . k , l , The structure of a nuclear pore complex (indicated by the boxes in ( m ), ( n )) becomes shifted in the vicinity of a pixel of high dark current, both in axial ( k ) and lateral ( l ) direction when neglecting individual pixel characteristics including thermal effects in the fitting pipeline. m Axial view of the region indicated in ( j ), also shown in Supplementary Video . n Lateral close up of the nuclear pore complex indicated in ( m ). As expected from the simulation, the shift features a high spatial dependence ( m , n ), which even changes its sign (indicated by the red arrows in n ).

    Article Snippet: U2OS NUP107-SNAP-tag cells (catalog no. 300294, CLS Cell Line Service, Eppelheim, Germany) were seeded onto clean 24 mm round glass coverslips and grown in phenol-red free Dulbecco’s Modified Eagle Medium growth medium (DMEM, Gibco no. 11880-02; 1x MEM NEAA, Gibco no. 11140-035; 1x GlutaMAX, Gibco no. 35050-038; 10% [v/v] fetal bovine serum, Gibco no. 10270-106).

    Techniques:

    Maps of dark current ( a ) and noise ( b ) at 50 ms single frame exposure time for an uncooled, industry-grade CMOS camera (characteristics shown in Fig. ). Simulations of astigmatism-based 3D PALM without explicit consideration of pixel-wise effects show a similar pattern in the localization bias, but of greater amplitude as compared to the cooled sCMOS camera (compare Fig. ), both laterally ( c ) and axially ( e ). Explicit application of CMOS-specific fitting largely removes the bias for PALM ( d , f ) as well as STORM and DNA-PAINT ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D PALM data of clathrin tagged with mEOS3.2 in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. j , Axial view of region indicated in i . k Experimental 3D STORM data of Nup107-SNAP, labeled with AF 647, in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. l Gallery showing lateral and axial views on individual nuclear pore complexes indicated in k . m The axial view on the region indicated in ( k ) shows two parallel lines from the nucleo- and cytoplasmic rings 57 nm apart. n First frame of experimental raw data from time-lapse TIRF imaging of AP-2 tagged with eGFP in live U373 cells recorded at 1000 ms single frame exposure time. o NCS corrected and ( p ) ACsN corrected frame. The entire time-lapse for ( n – p ) is shown in Supplementary Video . q The noise of a pixel of high dark current is strongly reduced via both approaches after appropriate characterization of the camera including thermal effects. The signal has been offset-corrected by the pixel value of the first frame from the time-lapse.

    Journal: Nature Communications

    Article Title: Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy

    doi: 10.1038/s41467-022-30907-2

    Figure Lengend Snippet: Maps of dark current ( a ) and noise ( b ) at 50 ms single frame exposure time for an uncooled, industry-grade CMOS camera (characteristics shown in Fig. ). Simulations of astigmatism-based 3D PALM without explicit consideration of pixel-wise effects show a similar pattern in the localization bias, but of greater amplitude as compared to the cooled sCMOS camera (compare Fig. ), both laterally ( c ) and axially ( e ). Explicit application of CMOS-specific fitting largely removes the bias for PALM ( d , f ) as well as STORM and DNA-PAINT ( g ) and restores the theoretically achievable root mean square error in the localizations ( h ). i , Experimental 3D PALM data of clathrin tagged with mEOS3.2 in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. j , Axial view of region indicated in i . k Experimental 3D STORM data of Nup107-SNAP, labeled with AF 647, in a U2OS cell using the same camera and applying CMOS-specific fitting including thermal effects. l Gallery showing lateral and axial views on individual nuclear pore complexes indicated in k . m The axial view on the region indicated in ( k ) shows two parallel lines from the nucleo- and cytoplasmic rings 57 nm apart. n First frame of experimental raw data from time-lapse TIRF imaging of AP-2 tagged with eGFP in live U373 cells recorded at 1000 ms single frame exposure time. o NCS corrected and ( p ) ACsN corrected frame. The entire time-lapse for ( n – p ) is shown in Supplementary Video . q The noise of a pixel of high dark current is strongly reduced via both approaches after appropriate characterization of the camera including thermal effects. The signal has been offset-corrected by the pixel value of the first frame from the time-lapse.

    Article Snippet: U2OS NUP107-SNAP-tag cells (catalog no. 300294, CLS Cell Line Service, Eppelheim, Germany) were seeded onto clean 24 mm round glass coverslips and grown in phenol-red free Dulbecco’s Modified Eagle Medium growth medium (DMEM, Gibco no. 11880-02; 1x MEM NEAA, Gibco no. 11140-035; 1x GlutaMAX, Gibco no. 35050-038; 10% [v/v] fetal bovine serum, Gibco no. 10270-106).

    Techniques: Labeling, Imaging