mcf-7 cells  (CLS Cell Lines Service GmbH)

Journal: PLOS ONE

Article Title: Glycemic load impacts the response of acquired resistance in breast cancer cells to chemotherapeutic drugs in vitro

doi: 10.1371/journal.pone.0311345

Figure Lengend Snippet: (a&b Top panel) Resistant MDA-4xAC and MDA-4xAC+4xPAC proliferated and produced more colonies compared to control cells under hyperglycemia (25 mM) and normal glucose level (5 mM). Resistant MCF7-4xAC and MCF7-4xAC+4xPAC proliferated and had fewer and smaller colonies than control MCF7 cells (bottom panel).

Article Snippet: MCF7 (300273) and MDA-MB-231 (300275) were purchased directly from Cell Lines Service CLS (Germany), which authenticates cell lines using the STR DNA analysis method.

Techniques: Produced, Control

Journal: PLOS ONE

Article Title: Glycemic load impacts the response of acquired resistance in breast cancer cells to chemotherapeutic drugs in vitro

doi: 10.1371/journal.pone.0311345

Figure Lengend Snippet: (a) Representative flow cytometry plots of MCF7 control and resistant variant cells under 25 mM, 5 mM, and 2 mM glucose levels. Numbers in quadrants show the mean percentages of necrotic (PI stained), early (FITC stained), and late apoptotic cells population (double stained PI&FITC). (b) Graphs summarizes the results of three independent flow cytometry experiments quantifying the mean ±SD of MDA control, MDA 4xAC, and MDA 4xAC+4xPac of necrotic cells in quadrants #1 late apoptotic cells in quadrants #2, and early apoptotic cells in quadrants #4. * represents p value less than 0.05.

Article Snippet: MCF7 (300273) and MDA-MB-231 (300275) were purchased directly from Cell Lines Service CLS (Germany), which authenticates cell lines using the STR DNA analysis method.

Techniques: Flow Cytometry, Control, Variant Assay, Staining

Journal: PLOS ONE

Article Title: Glycemic load impacts the response of acquired resistance in breast cancer cells to chemotherapeutic drugs in vitro

doi: 10.1371/journal.pone.0311345

Figure Lengend Snippet: (a) The Top graph displays MDA-MB-231 control and resistant cell invasion under three glucose levels (25 mM, 5 mM, and 2 mM). The bottom graph illustrates MCF7 control and resistant cell invasion. (b) Real-time PCR results for TP53 transcripts in MDA-MB-231 and MCF7 control and resistant cells under 25 mM, 5 mM, and 2 mM glucose. The experiments were replicated three times independently; significance was considered at P<0.05.

Article Snippet: MCF7 (300273) and MDA-MB-231 (300275) were purchased directly from Cell Lines Service CLS (Germany), which authenticates cell lines using the STR DNA analysis method.

Techniques: Control, Real-time Polymerase Chain Reaction

Journal: PLOS ONE

Article Title: Glycemic load impacts the response of acquired resistance in breast cancer cells to chemotherapeutic drugs in vitro

doi: 10.1371/journal.pone.0311345

Figure Lengend Snippet: Panel ( a) , western blot analysis shows the blots of NRP-1, P-NF-kB, NF-kB, P-AKT-473, AKT, and GAPDH of MDA-MB-231 control and resistant variants under 25 mM, 5 mM, and 2 mM of glucose. Panel ( b) shows the same proteins but in MCF7 control and resistant variants. Panel ( c) , western blot analysis shows the blots of IGF1R,P-GSK3β, GSK3β, P27 and GAPDH of MDA-MB-231 control and resistant variants under 25 mM, 5 mM, and 2 mM of glucose. Panel ( d) shows the same proteins but in MCF7 control and resistant variants. The graphs of the figures show the protein bands’ mean relative intensity.

Article Snippet: MCF7 (300273) and MDA-MB-231 (300275) were purchased directly from Cell Lines Service CLS (Germany), which authenticates cell lines using the STR DNA analysis method.

Techniques: Western Blot, Control