ht 29 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH ht 29 cells
    Ht 29 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ht 29 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH ht 29 cells
    Ht 29 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human colon cancer cells ht 29  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colon cancer cells ht 29
    Origanum majorana essential oil (OMEO) inhibits the cellular viability of <t>HT-29</t> cells. ( A ) Exponentially growing HT-29 colon cancer cells were treated with and without the indicated concentrations (0, 64, 128, 256, and 640 μg/mL) of OMEO for 6, 24, and 48 h. Viability was measured as described in Data represent the mean of six independent experiments carried out in triplicate. Values are represented as mean ± SD of n = 4 ( *** p < 0.001). ( B ) Morphological changes in OMEO-treated HT-29 cells. Morphological changes observed in the treated HT-29 cells after 6 h of treatment with the indicated concentration of OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×.
    Human Colon Cancer Cells Ht 29, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells"

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    Journal: Biomolecules

    doi: 10.3390/biom10030412

    Origanum majorana essential oil (OMEO) inhibits the cellular viability of HT-29 cells. ( A ) Exponentially growing HT-29 colon cancer cells were treated with and without the indicated concentrations (0, 64, 128, 256, and 640 μg/mL) of OMEO for 6, 24, and 48 h. Viability was measured as described in Data represent the mean of six independent experiments carried out in triplicate. Values are represented as mean ± SD of n = 4 ( *** p < 0.001). ( B ) Morphological changes in OMEO-treated HT-29 cells. Morphological changes observed in the treated HT-29 cells after 6 h of treatment with the indicated concentration of OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×.
    Figure Legend Snippet: Origanum majorana essential oil (OMEO) inhibits the cellular viability of HT-29 cells. ( A ) Exponentially growing HT-29 colon cancer cells were treated with and without the indicated concentrations (0, 64, 128, 256, and 640 μg/mL) of OMEO for 6, 24, and 48 h. Viability was measured as described in Data represent the mean of six independent experiments carried out in triplicate. Values are represented as mean ± SD of n = 4 ( *** p < 0.001). ( B ) Morphological changes in OMEO-treated HT-29 cells. Morphological changes observed in the treated HT-29 cells after 6 h of treatment with the indicated concentration of OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×.

    Techniques Used: Concentration Assay, Imaging

    OMEO inhibits HT-29 colony growth. ( A , B ) Inhibition of HT-29 colony growth by various concentrations of OMEO (0, 64, 128, and 256 μg/mL) was assessed by measuring the number of the colonies obtained in control and OMEO-treated plate, as described in Values are represented as mean ± SD of n = 3 (** p < 0.005). ( C ) HT-29 colonies were first allowed to form in normal media for 13 days as described in Formed colonies were then treated with or without increasing concentrations of OMEO, and allowed to grow for three more days before crystal violet staining. The size and morphology of the growing colonies was tracked over time with the EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( D ) Inhibition of colony growth was assessed by measuring the number and size (surface area) of the colonies obtained in control and OMEO-treated plates, as described in Data represent the mean of three independent experiments carried out in duplicate. Values are represented as mean ± SD of n = 3 ( * p < 0.05, ** p < 0.005).
    Figure Legend Snippet: OMEO inhibits HT-29 colony growth. ( A , B ) Inhibition of HT-29 colony growth by various concentrations of OMEO (0, 64, 128, and 256 μg/mL) was assessed by measuring the number of the colonies obtained in control and OMEO-treated plate, as described in Values are represented as mean ± SD of n = 3 (** p < 0.005). ( C ) HT-29 colonies were first allowed to form in normal media for 13 days as described in Formed colonies were then treated with or without increasing concentrations of OMEO, and allowed to grow for three more days before crystal violet staining. The size and morphology of the growing colonies was tracked over time with the EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( D ) Inhibition of colony growth was assessed by measuring the number and size (surface area) of the colonies obtained in control and OMEO-treated plates, as described in Data represent the mean of three independent experiments carried out in duplicate. Values are represented as mean ± SD of n = 3 ( * p < 0.05, ** p < 0.005).

    Techniques Used: Inhibition, Staining, Imaging

    Induction of caspase-8, -9, and -3-mediated apoptosis by OMEO in HT-29 cells. ( A ) Western blot analysis of cleaved PARP, caspase-8, -9, and -3 activation in HT-29 cells treated with increasing concentrations of OMEO (0, 64, 128, and 256 μg/mL) for 6 h. ( B ) Western blot quantification of TNF-α protein in OMEO-treated HT-29 cells.
    Figure Legend Snippet: Induction of caspase-8, -9, and -3-mediated apoptosis by OMEO in HT-29 cells. ( A ) Western blot analysis of cleaved PARP, caspase-8, -9, and -3 activation in HT-29 cells treated with increasing concentrations of OMEO (0, 64, 128, and 256 μg/mL) for 6 h. ( B ) Western blot quantification of TNF-α protein in OMEO-treated HT-29 cells.

    Techniques Used: Western Blot, Activation Assay

    OMEO induces autophagy associated with downregulation of mTOR/p70S6K in HT-29 cells. ( A ) Induction of autophagy by OMEO. Western blotting analysis of marker of autophagy, p62(SQSTM1), Beclin-1, and LC3 II in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration of OMEO (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in ( B ) Downregulation of the mTOR/p70S6K by OMEO. Western blot analysis for the phosphorylated and non-phosphorylated form of mTOR and p70S6K.
    Figure Legend Snippet: OMEO induces autophagy associated with downregulation of mTOR/p70S6K in HT-29 cells. ( A ) Induction of autophagy by OMEO. Western blotting analysis of marker of autophagy, p62(SQSTM1), Beclin-1, and LC3 II in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration of OMEO (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in ( B ) Downregulation of the mTOR/p70S6K by OMEO. Western blot analysis for the phosphorylated and non-phosphorylated form of mTOR and p70S6K.

    Techniques Used: Western Blot, Marker, Concentration Assay

    Protective autophagy and apoptotic cell death in HT-29 cells in response to OMEO. ( A ) Time-course analysis of LC3 II and cleaved PARP in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were determined by Western blot at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h) post-treatment. ( B ) Blockade of autophagy increases cell death, while inhibition of apoptosis promotes cell survival. HT-29 cells were pretreated with Z-VAD-FMK, 3-MA, or CQ and then incubated for 6 h with 256 μg/mL OMEO. Cell viability was determined as described in ( C ) Micrograph observation of HT-29 cells pretreated with or without pan-caspase inhibitor (Z-VAD-FMK) and 3-MA, as described above. ( D ) Western blotting analysis of cleaved PARP in OMEO cell pretreated with Z-VAD-FMK. ( E ) Western blotting analysis of cleaved caspase-3 with or without 3-MA or CQ.
    Figure Legend Snippet: Protective autophagy and apoptotic cell death in HT-29 cells in response to OMEO. ( A ) Time-course analysis of LC3 II and cleaved PARP in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were determined by Western blot at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h) post-treatment. ( B ) Blockade of autophagy increases cell death, while inhibition of apoptosis promotes cell survival. HT-29 cells were pretreated with Z-VAD-FMK, 3-MA, or CQ and then incubated for 6 h with 256 μg/mL OMEO. Cell viability was determined as described in ( C ) Micrograph observation of HT-29 cells pretreated with or without pan-caspase inhibitor (Z-VAD-FMK) and 3-MA, as described above. ( D ) Western blotting analysis of cleaved PARP in OMEO cell pretreated with Z-VAD-FMK. ( E ) Western blotting analysis of cleaved caspase-3 with or without 3-MA or CQ.

    Techniques Used: Western Blot, Inhibition, Incubation

    p38 MAPK-dependent activation of apoptosis in OMEO-treated cells. ( A ) OMEO activates p38 MAPK in HT-29 cells. Western blotting analysis of phosphorylated and total p38 in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were subjected to Western blot analysis. ( B ) Western blotting analysis of phosphorylated p38 in the presence of p38 inhibitors (SB 202190 and SB 203580). ( C ) Inhibition of p38 MAPK abrogates the OMEO-induced apoptotic cell death. HT-29 cells were pretreated with p38 inhibitors and cellular viability was determined as described in ( D ) Morphological changes observed in the treated HT-29 pretreated with p38 inhibitors prior the incubation with OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( E ) Western blotting analysis of cleaved PARP and cleaved caspase-3 in cell pretreated with and without SB 202190 and SB 203580.
    Figure Legend Snippet: p38 MAPK-dependent activation of apoptosis in OMEO-treated cells. ( A ) OMEO activates p38 MAPK in HT-29 cells. Western blotting analysis of phosphorylated and total p38 in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were subjected to Western blot analysis. ( B ) Western blotting analysis of phosphorylated p38 in the presence of p38 inhibitors (SB 202190 and SB 203580). ( C ) Inhibition of p38 MAPK abrogates the OMEO-induced apoptotic cell death. HT-29 cells were pretreated with p38 inhibitors and cellular viability was determined as described in ( D ) Morphological changes observed in the treated HT-29 pretreated with p38 inhibitors prior the incubation with OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( E ) Western blotting analysis of cleaved PARP and cleaved caspase-3 in cell pretreated with and without SB 202190 and SB 203580.

    Techniques Used: Activation Assay, Western Blot, Concentration Assay, Inhibition, Incubation, Imaging

    p38 MAPK-dependent caspase-dependent cleavage of p70S6K. ( A ) Time course measurement of p70S6K and cleaved caspase-3 in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were examined at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h). ( B ) Western blotting analysis of p70S6K and cleaved caspase-3 in cells pretreated with Z-VAD-FMK. ( C ) Western blot analysis of full-length and phosphorylated p70S6K in cells pretreated with SB 202190 and SB 203580.
    Figure Legend Snippet: p38 MAPK-dependent caspase-dependent cleavage of p70S6K. ( A ) Time course measurement of p70S6K and cleaved caspase-3 in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were examined at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h). ( B ) Western blotting analysis of p70S6K and cleaved caspase-3 in cells pretreated with Z-VAD-FMK. ( C ) Western blot analysis of full-length and phosphorylated p70S6K in cells pretreated with SB 202190 and SB 203580.

    Techniques Used: Western Blot

    DNA damage in OMEO-treated HT-29 cells. ( A ) Accumulation of γH2AX, a marker of DNA damage, in OMEO-treated cells. HT-29 cells were treated with and without increasing concentrations of OMEO for 6 h and DNA damage was analyzed by Western blot, by determining the level of γH2AX accumulation. ( B ) Western blotting analysis of γH2AX in cells pretreated with and without Z-VAD-FMK. ( C ) Western blotting analysis of γH2AX in cells pretreated with and without SB 202190 and SB 203580.
    Figure Legend Snippet: DNA damage in OMEO-treated HT-29 cells. ( A ) Accumulation of γH2AX, a marker of DNA damage, in OMEO-treated cells. HT-29 cells were treated with and without increasing concentrations of OMEO for 6 h and DNA damage was analyzed by Western blot, by determining the level of γH2AX accumulation. ( B ) Western blotting analysis of γH2AX in cells pretreated with and without Z-VAD-FMK. ( C ) Western blotting analysis of γH2AX in cells pretreated with and without SB 202190 and SB 203580.

    Techniques Used: Marker, Western Blot

    human colon cancer cells ht 29  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colon cancer cells ht 29
    Origanum majorana ethanolic extract inhibits cellular viability of colorectal cancer cells. (A) Exponentially growing <t>HT-29</t> and (B) Caco-2 colon cancer cells were treated with and without of various concentration (0, 150, 300, 450, and 600 μg/mL) OME for 24 and 48 h. Viability was measured using a colorimetric assay as described in section Materials and Methods. Values are represented as mean ± SD of n = 4 (* p < 0.05 and *** p < 0.001). (C) HT-29 cells were exposed to OME for 24 and 48 h and number of viable cells, using a fluorescent dye, was monitored as described in section Materials and Methods using the Muse Cell Analyzer (Millipore). Data represent the mean ± SD of n = 3 carried out in triplicate.
    Human Colon Cancer Cells Ht 29, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Origanum majorana Ethanolic Extract Promotes Colorectal Cancer Cell Death by Triggering Abortive Autophagy and Activation of the Extrinsic Apoptotic Pathway"

    Article Title: Origanum majorana Ethanolic Extract Promotes Colorectal Cancer Cell Death by Triggering Abortive Autophagy and Activation of the Extrinsic Apoptotic Pathway

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2019.00795

    Origanum majorana ethanolic extract inhibits cellular viability of colorectal cancer cells. (A) Exponentially growing HT-29 and (B) Caco-2 colon cancer cells were treated with and without of various concentration (0, 150, 300, 450, and 600 μg/mL) OME for 24 and 48 h. Viability was measured using a colorimetric assay as described in section Materials and Methods. Values are represented as mean ± SD of n = 4 (* p < 0.05 and *** p < 0.001). (C) HT-29 cells were exposed to OME for 24 and 48 h and number of viable cells, using a fluorescent dye, was monitored as described in section Materials and Methods using the Muse Cell Analyzer (Millipore). Data represent the mean ± SD of n = 3 carried out in triplicate.
    Figure Legend Snippet: Origanum majorana ethanolic extract inhibits cellular viability of colorectal cancer cells. (A) Exponentially growing HT-29 and (B) Caco-2 colon cancer cells were treated with and without of various concentration (0, 150, 300, 450, and 600 μg/mL) OME for 24 and 48 h. Viability was measured using a colorimetric assay as described in section Materials and Methods. Values are represented as mean ± SD of n = 4 (* p < 0.05 and *** p < 0.001). (C) HT-29 cells were exposed to OME for 24 and 48 h and number of viable cells, using a fluorescent dye, was monitored as described in section Materials and Methods using the Muse Cell Analyzer (Millipore). Data represent the mean ± SD of n = 3 carried out in triplicate.

    Techniques Used: Concentration Assay, Colorimetric Assay

    Origanum majorana inhibits HT-29 colony growth. (A–C) Inhibition of formed HT-29 colony growth by various concentrations of OME (0, 150, 300, 450, and 600 μg/mL) was assessed by measuring the number and average size (surface area) of the colonies obtained in control and OME-treated plate as described in section Materials and methods. Values are represented as mean ± SD of n = 3 (* p < 0.05 and ** p < 0.005).
    Figure Legend Snippet: Origanum majorana inhibits HT-29 colony growth. (A–C) Inhibition of formed HT-29 colony growth by various concentrations of OME (0, 150, 300, 450, and 600 μg/mL) was assessed by measuring the number and average size (surface area) of the colonies obtained in control and OME-treated plate as described in section Materials and methods. Values are represented as mean ± SD of n = 3 (* p < 0.05 and ** p < 0.005).

    Techniques Used: Inhibition

    OME induces a mitotic arrest in HT-29 cells. (A,B) Cell cycle distribution analysis in HT-29 cells treated with and without OME (0, 150, 300, 450, and 600 μg/mL) for 24 h. Values are represented as mean ± SD of n = 3 (* p < 0.05, ** p < 0.005, and *** p < 0.001). (C) Alteration in proteins associated with cell cycle regulation in OME-treated HT-29 cells.
    Figure Legend Snippet: OME induces a mitotic arrest in HT-29 cells. (A,B) Cell cycle distribution analysis in HT-29 cells treated with and without OME (0, 150, 300, 450, and 600 μg/mL) for 24 h. Values are represented as mean ± SD of n = 3 (* p < 0.05, ** p < 0.005, and *** p < 0.001). (C) Alteration in proteins associated with cell cycle regulation in OME-treated HT-29 cells.

    Techniques Used:

    Activation of extrinsic apoptotic pathway and upregulation of TNF-α in OME-treated HT-29 cells. (A) Western blot analysis of caspase 3, 7, and 8 activation and PARP cleavage in HT-29 cells. Cells were treated with or without increasing concentration (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h, then whole cell proteins were extracted and subjected to Western blot analysis for the markers of apoptosis (B) Western blot analysis of TNF-α (C) Western blot analysis of cleaved PARP in cells pretreated for 1 h with and without Z-VAD-FMK (50 μM) followed by treatment with OME (450 μg/mL) for 48 h. (D) Inhibition of apoptosis has a minimal effect of OME-induced cell death. HT-29 cells were pretreated with Z-VAD-FMK as described above and then treated for 48 h with 450 μg/mL OME. Cell viability was determined as described in section Material and Methods. Values are represented as mean ± SD of n = 3 (* p < 0.05 and *** p < 0.001).
    Figure Legend Snippet: Activation of extrinsic apoptotic pathway and upregulation of TNF-α in OME-treated HT-29 cells. (A) Western blot analysis of caspase 3, 7, and 8 activation and PARP cleavage in HT-29 cells. Cells were treated with or without increasing concentration (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h, then whole cell proteins were extracted and subjected to Western blot analysis for the markers of apoptosis (B) Western blot analysis of TNF-α (C) Western blot analysis of cleaved PARP in cells pretreated for 1 h with and without Z-VAD-FMK (50 μM) followed by treatment with OME (450 μg/mL) for 48 h. (D) Inhibition of apoptosis has a minimal effect of OME-induced cell death. HT-29 cells were pretreated with Z-VAD-FMK as described above and then treated for 48 h with 450 μg/mL OME. Cell viability was determined as described in section Material and Methods. Values are represented as mean ± SD of n = 3 (* p < 0.05 and *** p < 0.001).

    Techniques Used: Activation Assay, Western Blot, Concentration Assay, Inhibition

    OME induces abortive autophagy in HT-29 cells. Western blotting analysis of LC3II, p62(SQSTM1), and Beclin-1 expression OME-treated HT-29 cells. Cells were treated with or without increasing concentration (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in section Materials and Methods, for LC3II, 62(SQSTM1), and Beclin-1.
    Figure Legend Snippet: OME induces abortive autophagy in HT-29 cells. Western blotting analysis of LC3II, p62(SQSTM1), and Beclin-1 expression OME-treated HT-29 cells. Cells were treated with or without increasing concentration (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in section Materials and Methods, for LC3II, 62(SQSTM1), and Beclin-1.

    Techniques Used: Western Blot, Expressing, Concentration Assay

    OME induces DNA damage in response to OME treatment in HT-29 cells. HT-29 cells were treated with increasing concentrations (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h. DNA damage was examined by western blotting by measuring the level of phosphorylated H2AX.
    Figure Legend Snippet: OME induces DNA damage in response to OME treatment in HT-29 cells. HT-29 cells were treated with increasing concentrations (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h. DNA damage was examined by western blotting by measuring the level of phosphorylated H2AX.

    Techniques Used: Western Blot

    DNA damage and autophagy precedes apoptosis in OME-treated HT-29 cells. (A) Time-course analysis, by Western blotting, of PARP and caspase 8 cleavage, LC3-II, p62 (SQSTM1), γH2AX, and H3pser10 accumulation in OME-treated HT-29 cells. Cells were treated with 450 μg/mL OME and proteins were extracted at the indicated time-points (0, 4, 8, 24, and 48 h) as described in section Materials and Methods. (B) Western blot analysis of γH2AX accumulation in HT-29 cells pre-treated with 3MA. Cells were pretreated with or without 3-MA (5 mM) for 1 h and then OME (450 μg/mL) was added, and cells were incubated for 48 h.
    Figure Legend Snippet: DNA damage and autophagy precedes apoptosis in OME-treated HT-29 cells. (A) Time-course analysis, by Western blotting, of PARP and caspase 8 cleavage, LC3-II, p62 (SQSTM1), γH2AX, and H3pser10 accumulation in OME-treated HT-29 cells. Cells were treated with 450 μg/mL OME and proteins were extracted at the indicated time-points (0, 4, 8, 24, and 48 h) as described in section Materials and Methods. (B) Western blot analysis of γH2AX accumulation in HT-29 cells pre-treated with 3MA. Cells were pretreated with or without 3-MA (5 mM) for 1 h and then OME (450 μg/mL) was added, and cells were incubated for 48 h.

    Techniques Used: Western Blot, Incubation

    Inhibition of autophagy decreases OME-induced cell death in HT-29 cells. (A) Analysis of LC3-II and cleaved PARP accumulation in HT-29 cells pre-treated with 3-MA. Cells were pretreated with or without 3-MA (5 mM) for 1 h and then OME (450 μg/mL) was added, and cells were incubated for 48 h. (B) Inhibition of autophagy reduces cell death induced by OME. HT-29 cells were pretreated with 3-MA for 1 h and then for 48 h with 450 μg/mL OME. Cell viability was determined as described in Material and Methods. Values are represented as mean ± SD of n = 3 (*** p < 0.001).
    Figure Legend Snippet: Inhibition of autophagy decreases OME-induced cell death in HT-29 cells. (A) Analysis of LC3-II and cleaved PARP accumulation in HT-29 cells pre-treated with 3-MA. Cells were pretreated with or without 3-MA (5 mM) for 1 h and then OME (450 μg/mL) was added, and cells were incubated for 48 h. (B) Inhibition of autophagy reduces cell death induced by OME. HT-29 cells were pretreated with 3-MA for 1 h and then for 48 h with 450 μg/mL OME. Cell viability was determined as described in Material and Methods. Values are represented as mean ± SD of n = 3 (*** p < 0.001).

    Techniques Used: Inhibition, Incubation

    Downregulation of survivin by OME in HT-29 cells. HT-29 cells were treated with increasing concentrations (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h and the level of survivin was assessed by Western blotting.
    Figure Legend Snippet: Downregulation of survivin by OME in HT-29 cells. HT-29 cells were treated with increasing concentrations (0, 150, 300, 450, and 600 μg/mL) of OME for 48 h and the level of survivin was assessed by Western blotting.

    Techniques Used: Western Blot

    ht 29  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH ht 29
    Ht 29, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH ht 29 cells
    Ht 29 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH human colon cancer cells ht 29
    Origanum majorana essential oil (OMEO) inhibits the cellular viability of <t>HT-29</t> cells. ( A ) Exponentially growing HT-29 colon cancer cells were treated with and without the indicated concentrations (0, 64, 128, 256, and 640 μg/mL) of OMEO for 6, 24, and 48 h. Viability was measured as described in Data represent the mean of six independent experiments carried out in triplicate. Values are represented as mean ± SD of n = 4 ( *** p < 0.001). ( B ) Morphological changes in OMEO-treated HT-29 cells. Morphological changes observed in the treated HT-29 cells after 6 h of treatment with the indicated concentration of OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×.
    Human Colon Cancer Cells Ht 29, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human colon cancer cells ht 29/product/CLS Cell Lines Service GmbH
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human colon cancer cells ht 29 - by Bioz Stars, 2024-04
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    CLS Cell Lines Service GmbH ht 29
    Origanum majorana essential oil (OMEO) inhibits the cellular viability of <t>HT-29</t> cells. ( A ) Exponentially growing HT-29 colon cancer cells were treated with and without the indicated concentrations (0, 64, 128, 256, and 640 μg/mL) of OMEO for 6, 24, and 48 h. Viability was measured as described in Data represent the mean of six independent experiments carried out in triplicate. Values are represented as mean ± SD of n = 4 ( *** p < 0.001). ( B ) Morphological changes in OMEO-treated HT-29 cells. Morphological changes observed in the treated HT-29 cells after 6 h of treatment with the indicated concentration of OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×.
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    Origanum majorana essential oil (OMEO) inhibits the cellular viability of HT-29 cells. ( A ) Exponentially growing HT-29 colon cancer cells were treated with and without the indicated concentrations (0, 64, 128, 256, and 640 μg/mL) of OMEO for 6, 24, and 48 h. Viability was measured as described in Data represent the mean of six independent experiments carried out in triplicate. Values are represented as mean ± SD of n = 4 ( *** p < 0.001). ( B ) Morphological changes in OMEO-treated HT-29 cells. Morphological changes observed in the treated HT-29 cells after 6 h of treatment with the indicated concentration of OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×.

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: Origanum majorana essential oil (OMEO) inhibits the cellular viability of HT-29 cells. ( A ) Exponentially growing HT-29 colon cancer cells were treated with and without the indicated concentrations (0, 64, 128, 256, and 640 μg/mL) of OMEO for 6, 24, and 48 h. Viability was measured as described in Data represent the mean of six independent experiments carried out in triplicate. Values are represented as mean ± SD of n = 4 ( *** p < 0.001). ( B ) Morphological changes in OMEO-treated HT-29 cells. Morphological changes observed in the treated HT-29 cells after 6 h of treatment with the indicated concentration of OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×.

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Concentration Assay, Imaging

    OMEO inhibits HT-29 colony growth. ( A , B ) Inhibition of HT-29 colony growth by various concentrations of OMEO (0, 64, 128, and 256 μg/mL) was assessed by measuring the number of the colonies obtained in control and OMEO-treated plate, as described in Values are represented as mean ± SD of n = 3 (** p < 0.005). ( C ) HT-29 colonies were first allowed to form in normal media for 13 days as described in Formed colonies were then treated with or without increasing concentrations of OMEO, and allowed to grow for three more days before crystal violet staining. The size and morphology of the growing colonies was tracked over time with the EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( D ) Inhibition of colony growth was assessed by measuring the number and size (surface area) of the colonies obtained in control and OMEO-treated plates, as described in Data represent the mean of three independent experiments carried out in duplicate. Values are represented as mean ± SD of n = 3 ( * p < 0.05, ** p < 0.005).

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: OMEO inhibits HT-29 colony growth. ( A , B ) Inhibition of HT-29 colony growth by various concentrations of OMEO (0, 64, 128, and 256 μg/mL) was assessed by measuring the number of the colonies obtained in control and OMEO-treated plate, as described in Values are represented as mean ± SD of n = 3 (** p < 0.005). ( C ) HT-29 colonies were first allowed to form in normal media for 13 days as described in Formed colonies were then treated with or without increasing concentrations of OMEO, and allowed to grow for three more days before crystal violet staining. The size and morphology of the growing colonies was tracked over time with the EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( D ) Inhibition of colony growth was assessed by measuring the number and size (surface area) of the colonies obtained in control and OMEO-treated plates, as described in Data represent the mean of three independent experiments carried out in duplicate. Values are represented as mean ± SD of n = 3 ( * p < 0.05, ** p < 0.005).

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Inhibition, Staining, Imaging

    Induction of caspase-8, -9, and -3-mediated apoptosis by OMEO in HT-29 cells. ( A ) Western blot analysis of cleaved PARP, caspase-8, -9, and -3 activation in HT-29 cells treated with increasing concentrations of OMEO (0, 64, 128, and 256 μg/mL) for 6 h. ( B ) Western blot quantification of TNF-α protein in OMEO-treated HT-29 cells.

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: Induction of caspase-8, -9, and -3-mediated apoptosis by OMEO in HT-29 cells. ( A ) Western blot analysis of cleaved PARP, caspase-8, -9, and -3 activation in HT-29 cells treated with increasing concentrations of OMEO (0, 64, 128, and 256 μg/mL) for 6 h. ( B ) Western blot quantification of TNF-α protein in OMEO-treated HT-29 cells.

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Western Blot, Activation Assay

    OMEO induces autophagy associated with downregulation of mTOR/p70S6K in HT-29 cells. ( A ) Induction of autophagy by OMEO. Western blotting analysis of marker of autophagy, p62(SQSTM1), Beclin-1, and LC3 II in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration of OMEO (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in ( B ) Downregulation of the mTOR/p70S6K by OMEO. Western blot analysis for the phosphorylated and non-phosphorylated form of mTOR and p70S6K.

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: OMEO induces autophagy associated with downregulation of mTOR/p70S6K in HT-29 cells. ( A ) Induction of autophagy by OMEO. Western blotting analysis of marker of autophagy, p62(SQSTM1), Beclin-1, and LC3 II in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration of OMEO (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in ( B ) Downregulation of the mTOR/p70S6K by OMEO. Western blot analysis for the phosphorylated and non-phosphorylated form of mTOR and p70S6K.

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Western Blot, Marker, Concentration Assay

    Protective autophagy and apoptotic cell death in HT-29 cells in response to OMEO. ( A ) Time-course analysis of LC3 II and cleaved PARP in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were determined by Western blot at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h) post-treatment. ( B ) Blockade of autophagy increases cell death, while inhibition of apoptosis promotes cell survival. HT-29 cells were pretreated with Z-VAD-FMK, 3-MA, or CQ and then incubated for 6 h with 256 μg/mL OMEO. Cell viability was determined as described in ( C ) Micrograph observation of HT-29 cells pretreated with or without pan-caspase inhibitor (Z-VAD-FMK) and 3-MA, as described above. ( D ) Western blotting analysis of cleaved PARP in OMEO cell pretreated with Z-VAD-FMK. ( E ) Western blotting analysis of cleaved caspase-3 with or without 3-MA or CQ.

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: Protective autophagy and apoptotic cell death in HT-29 cells in response to OMEO. ( A ) Time-course analysis of LC3 II and cleaved PARP in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were determined by Western blot at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h) post-treatment. ( B ) Blockade of autophagy increases cell death, while inhibition of apoptosis promotes cell survival. HT-29 cells were pretreated with Z-VAD-FMK, 3-MA, or CQ and then incubated for 6 h with 256 μg/mL OMEO. Cell viability was determined as described in ( C ) Micrograph observation of HT-29 cells pretreated with or without pan-caspase inhibitor (Z-VAD-FMK) and 3-MA, as described above. ( D ) Western blotting analysis of cleaved PARP in OMEO cell pretreated with Z-VAD-FMK. ( E ) Western blotting analysis of cleaved caspase-3 with or without 3-MA or CQ.

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Western Blot, Inhibition, Incubation

    p38 MAPK-dependent activation of apoptosis in OMEO-treated cells. ( A ) OMEO activates p38 MAPK in HT-29 cells. Western blotting analysis of phosphorylated and total p38 in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were subjected to Western blot analysis. ( B ) Western blotting analysis of phosphorylated p38 in the presence of p38 inhibitors (SB 202190 and SB 203580). ( C ) Inhibition of p38 MAPK abrogates the OMEO-induced apoptotic cell death. HT-29 cells were pretreated with p38 inhibitors and cellular viability was determined as described in ( D ) Morphological changes observed in the treated HT-29 pretreated with p38 inhibitors prior the incubation with OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( E ) Western blotting analysis of cleaved PARP and cleaved caspase-3 in cell pretreated with and without SB 202190 and SB 203580.

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: p38 MAPK-dependent activation of apoptosis in OMEO-treated cells. ( A ) OMEO activates p38 MAPK in HT-29 cells. Western blotting analysis of phosphorylated and total p38 in OMEO-treated HT-29 cells. Cells were treated with or without an increasing concentration (0, 64, 128, and 256 μg/mL) for 6 h, then whole cell proteins were subjected to Western blot analysis. ( B ) Western blotting analysis of phosphorylated p38 in the presence of p38 inhibitors (SB 202190 and SB 203580). ( C ) Inhibition of p38 MAPK abrogates the OMEO-induced apoptotic cell death. HT-29 cells were pretreated with p38 inhibitors and cellular viability was determined as described in ( D ) Morphological changes observed in the treated HT-29 pretreated with p38 inhibitors prior the incubation with OMEO. Cells were observed under EVOS XL Core Cell Imaging System (Life Technologies) at 400×. ( E ) Western blotting analysis of cleaved PARP and cleaved caspase-3 in cell pretreated with and without SB 202190 and SB 203580.

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Activation Assay, Western Blot, Concentration Assay, Inhibition, Incubation, Imaging

    p38 MAPK-dependent caspase-dependent cleavage of p70S6K. ( A ) Time course measurement of p70S6K and cleaved caspase-3 in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were examined at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h). ( B ) Western blotting analysis of p70S6K and cleaved caspase-3 in cells pretreated with Z-VAD-FMK. ( C ) Western blot analysis of full-length and phosphorylated p70S6K in cells pretreated with SB 202190 and SB 203580.

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: p38 MAPK-dependent caspase-dependent cleavage of p70S6K. ( A ) Time course measurement of p70S6K and cleaved caspase-3 in OMEO-treated HT-29 cells. Cells were treated with 256 μg/mL OMEO and protein levels were examined at different time points (0, 5 min, 15 min, 30 min, 1 h, and 3 h). ( B ) Western blotting analysis of p70S6K and cleaved caspase-3 in cells pretreated with Z-VAD-FMK. ( C ) Western blot analysis of full-length and phosphorylated p70S6K in cells pretreated with SB 202190 and SB 203580.

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Western Blot

    DNA damage in OMEO-treated HT-29 cells. ( A ) Accumulation of γH2AX, a marker of DNA damage, in OMEO-treated cells. HT-29 cells were treated with and without increasing concentrations of OMEO for 6 h and DNA damage was analyzed by Western blot, by determining the level of γH2AX accumulation. ( B ) Western blotting analysis of γH2AX in cells pretreated with and without Z-VAD-FMK. ( C ) Western blotting analysis of γH2AX in cells pretreated with and without SB 202190 and SB 203580.

    Journal: Biomolecules

    Article Title: Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

    doi: 10.3390/biom10030412

    Figure Lengend Snippet: DNA damage in OMEO-treated HT-29 cells. ( A ) Accumulation of γH2AX, a marker of DNA damage, in OMEO-treated cells. HT-29 cells were treated with and without increasing concentrations of OMEO for 6 h and DNA damage was analyzed by Western blot, by determining the level of γH2AX accumulation. ( B ) Western blotting analysis of γH2AX in cells pretreated with and without Z-VAD-FMK. ( C ) Western blotting analysis of γH2AX in cells pretreated with and without SB 202190 and SB 203580.

    Article Snippet: Human colon cancer cells HT-29 (Cat# 300215) were purchased from CLS (Cell Lines Service, Eppelheim, Germany) and were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin (Hyclone, Cramlington, UK).

    Techniques: Marker, Western Blot