hbl 52 meningioma  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH hbl 52 meningioma
    Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in <t>HBL-52</t> meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also <xref ref-type=Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ). " width="250" height="auto" />
    Hbl 52 Meningioma, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hbl 52 meningioma - by Bioz Stars, 2024-05
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    1) Product Images from "Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression"

    Article Title: Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression

    Journal: iScience

    doi: 10.1016/j.isci.2022.104839

    Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also <xref ref-type=Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ). " title="... FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ).

    Techniques Used: Expressing, Transfection, Purification, Amplification, Quantitative RT-PCR, RNA Sequencing Assay, Transduction, Recombinant, Cell Counting, CCK-8 Assay


    Figure Legend Snippet:

    Techniques Used: Produced, Recombinant, RNA Sequencing Assay, Transfection, Mutagenesis, Luciferase, Reporter Gene Assay, CCK-8 Assay, Proliferation Assay, Cytotoxicity Assay, Expressing, Functional Assay, TaqMan Assay, Plasmid Preparation, Software

    hbl 52 meningioma  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH hbl 52 meningioma
    Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in <t>HBL-52</t> meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also <xref ref-type=Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ). " width="250" height="auto" />
    Hbl 52 Meningioma, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbl 52 meningioma/product/CLS Cell Lines Service GmbH
    Average 92 stars, based on 1 article reviews
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    1) Product Images from "Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression"

    Article Title: Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression

    Journal: iScience

    doi: 10.1016/j.isci.2022.104839

    Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also <xref ref-type=Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ). " title="... FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ).

    Techniques Used: Expressing, Transfection, Purification, Amplification, Quantitative RT-PCR, RNA Sequencing Assay, Transduction, Recombinant, Cell Counting, CCK-8 Assay


    Figure Legend Snippet:

    Techniques Used: Produced, Recombinant, RNA Sequencing Assay, Transfection, Mutagenesis, Luciferase, Reporter Gene Assay, CCK-8 Assay, Proliferation Assay, Cytotoxicity Assay, Expressing, Functional Assay, TaqMan Assay, Plasmid Preparation, Software

    cls  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH cls

    Cls, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression"

    Article Title: Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression

    Journal: iScience

    doi: 10.1016/j.isci.2022.104839


    Figure Legend Snippet:

    Techniques Used: Produced, Recombinant, RNA Sequencing Assay, Transfection, Mutagenesis, Luciferase, Reporter Gene Assay, CCK-8 Assay, Proliferation Assay, Cytotoxicity Assay, Expressing, Functional Assay, TaqMan Assay, Plasmid Preparation, Software

    benign cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH benign cells
    Benign Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tissues hbl 52 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH tissues hbl 52 cells
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    Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also <xref ref-type=Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ). " width="100%" height="100%">

    Journal: iScience

    Article Title: Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression

    doi: 10.1016/j.isci.2022.104839

    Figure Lengend Snippet: Meningeal cells display KLF4 K409Q -dependent FGF3 transcription and proliferate in response to FGF3 (A) FGF3 and TRH mRNA expression in HBL-52 meningioma cells transfected with KLF4- or KLF4 K409Q -expressing plasmids. Total RNA was purified 48 h posttransfection, and mRNA was amplified by RT-qPCR using GAPDH as internal control. Copy numbers were calculated per 10 6 copies of GAPDH mRNA in the same sample. (B) PCA Plot of RNA-seq meta-analysis in HMCs transduced with virus expressing KLF4 or KLF4 K409Q proteins ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156211 . Clustering of samples shows batch effect: instead of clustering by conditions, the samples clustered by their Sample ID (i.e., 1, 2, 3, 4). See also Figure S5 A. (C) Scatter plot showing activated and repressed DE genes in meta-analysis of RNA-seq in HMCs. FGF3 and TRH are marked by arrows. See also Venn diagrams in Figure S5 B. (D) Proliferation response of meningioma cell line HBL-52 to recombinant FGF3, FGF1, and EGF. Cells were treated with human recombinant FGF3 or FGF1 at 0.1 μg/mL in the presence of 1 μg/mL of heparin or EGF at 5 ng/mL final concentrations in complete medium. Cell proliferation was measured on different days poststimulation (as marked) by Absorbance (OD value at 450 nm) using a Cell Counting Kit-8 [CCK-8] as described in . Assay was set up in 96-well plates (starting at 1,000 cells/well), with eight replicates for each condition and repeated three times. Data are represented as mean ± SD (see also Figure S5 , Tables S4 and ).

    Article Snippet: HBL-52 meningioma, benign cells , CLS Cell Lines Service GmbH , CLS Cat#300188/p692_HBL-52; RRID: CVCL_4220.

    Techniques: Expressing, Transfection, Purification, Amplification, Quantitative RT-PCR, RNA Sequencing Assay, Transduction, Recombinant, Cell Counting, CCK-8 Assay

    Journal: iScience

    Article Title: Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression

    doi: 10.1016/j.isci.2022.104839

    Figure Lengend Snippet:

    Article Snippet: HBL-52 meningioma, benign cells , CLS Cell Lines Service GmbH , CLS Cat#300188/p692_HBL-52; RRID: CVCL_4220.

    Techniques: Produced, Recombinant, RNA Sequencing Assay, Transfection, Mutagenesis, Luciferase, Reporter Gene Assay, CCK-8 Assay, Proliferation Assay, Cytotoxicity Assay, Expressing, Functional Assay, TaqMan Assay, Plasmid Preparation, Software

    Journal: iScience

    Article Title: Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expression

    doi: 10.1016/j.isci.2022.104839

    Figure Lengend Snippet:

    Article Snippet: HBL-52 meningioma, benign cells , CLS Cell Lines Service GmbH , CLS Cat#300188/p692_HBL-52; RRID: CVCL_4220.

    Techniques: Produced, Recombinant, RNA Sequencing Assay, Transfection, Mutagenesis, Luciferase, Reporter Gene Assay, CCK-8 Assay, Proliferation Assay, Cytotoxicity Assay, Expressing, Functional Assay, TaqMan Assay, Plasmid Preparation, Software