dmem  (ATCC)


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    Structured Review

    ATCC dmem
    c-Met inhibitors repressed cell growth and c-Met receptor phosphorylation in PC-3 and DU-145 prostate cancer cells . ( A ) PC-3 cells were cultured with <t>DMEM</t> supplemented with 5% <t>FBS.</t> The c-Met inhibitor, PHA-665752 or PF-2341066, dissolved in DMSO was added to the growth media at the indicated concentration. Cells were harvested and analyzed by the MTS assay at indicated time-points. Absorbance at 490 nm was measured in triplicate samples. The data represent the mean ± S.D. of three independent experiments. ( B ) The similar MTS assays were carried out in DU-145 cells. ( C ) Approximately 50 PC-3 or DU-145 cells per well were plated in quadruplicate in 6-well plates for 24 hr, and then PHA-665752 or PF-2341066 was added to cells. Cells were allowed to grow for 10 days then fixed and stained with crystal violet. ( D ) PC-3 or DU-145 cells were cultured with 5% FBS-DMEM and were then exposed for 3 hours to DMSO alone, 0.5 μM of PHA-665752, or 0.5 μM of PF-2341066 dissolved in DMSO. Whole cell lysates were prepared from the above cells for immunoblotting with c-Met or phosphorylated c-Met antibody, as well as the β-tubulin antibody as a loading control.
    Dmem, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem/product/ATCC
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dmem - by Bioz Stars, 2022-10
    99/100 stars

    Images

    1) Product Images from "Efficacy of c-Met inhibitor for advanced prostate cancer"

    Article Title: Efficacy of c-Met inhibitor for advanced prostate cancer

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-10-556

    c-Met inhibitors repressed cell growth and c-Met receptor phosphorylation in PC-3 and DU-145 prostate cancer cells . ( A ) PC-3 cells were cultured with DMEM supplemented with 5% FBS. The c-Met inhibitor, PHA-665752 or PF-2341066, dissolved in DMSO was added to the growth media at the indicated concentration. Cells were harvested and analyzed by the MTS assay at indicated time-points. Absorbance at 490 nm was measured in triplicate samples. The data represent the mean ± S.D. of three independent experiments. ( B ) The similar MTS assays were carried out in DU-145 cells. ( C ) Approximately 50 PC-3 or DU-145 cells per well were plated in quadruplicate in 6-well plates for 24 hr, and then PHA-665752 or PF-2341066 was added to cells. Cells were allowed to grow for 10 days then fixed and stained with crystal violet. ( D ) PC-3 or DU-145 cells were cultured with 5% FBS-DMEM and were then exposed for 3 hours to DMSO alone, 0.5 μM of PHA-665752, or 0.5 μM of PF-2341066 dissolved in DMSO. Whole cell lysates were prepared from the above cells for immunoblotting with c-Met or phosphorylated c-Met antibody, as well as the β-tubulin antibody as a loading control.
    Figure Legend Snippet: c-Met inhibitors repressed cell growth and c-Met receptor phosphorylation in PC-3 and DU-145 prostate cancer cells . ( A ) PC-3 cells were cultured with DMEM supplemented with 5% FBS. The c-Met inhibitor, PHA-665752 or PF-2341066, dissolved in DMSO was added to the growth media at the indicated concentration. Cells were harvested and analyzed by the MTS assay at indicated time-points. Absorbance at 490 nm was measured in triplicate samples. The data represent the mean ± S.D. of three independent experiments. ( B ) The similar MTS assays were carried out in DU-145 cells. ( C ) Approximately 50 PC-3 or DU-145 cells per well were plated in quadruplicate in 6-well plates for 24 hr, and then PHA-665752 or PF-2341066 was added to cells. Cells were allowed to grow for 10 days then fixed and stained with crystal violet. ( D ) PC-3 or DU-145 cells were cultured with 5% FBS-DMEM and were then exposed for 3 hours to DMSO alone, 0.5 μM of PHA-665752, or 0.5 μM of PF-2341066 dissolved in DMSO. Whole cell lysates were prepared from the above cells for immunoblotting with c-Met or phosphorylated c-Met antibody, as well as the β-tubulin antibody as a loading control.

    Techniques Used: Cell Culture, Concentration Assay, MTS Assay, Staining

    2) Product Images from "Boosting Protein Encapsulation through Lewis-Acid-Mediated Metal–Organic Framework Mineralization: Toward Effective Intracellular Delivery"

    Article Title: Boosting Protein Encapsulation through Lewis-Acid-Mediated Metal–Organic Framework Mineralization: Toward Effective Intracellular Delivery

    Journal: Chemistry of Materials

    doi: 10.1021/acs.chemmater.2c01338

    (A) Mechanism of action of the Mb@MIL-100(Fe) biocomposite in hypoxic cells. Cisplatin (CPT) treatment results in the survival of hypoxic A549 cells and apoptosis of normoxic A549 cells. Incubating hypoxic A549 cells with Mb@MIL-100(Fe) -2 induces the intracellular release of Mb protein and the subsequent enhancement of intracellular O 2 , prompting the apoptosis of hypoxic A549 cells. (B) Representative confocal images of normoxic A549 cells. Cells were incubated under each condition in DMEM +10% FBS in 20% O 2 at 37 °C for different time points, fixed with 4% PFA, and images were acquired by using a confocal microscope Olympus FV1000. (C) Quantification of at least 10 images of each condition. Data represent mean ± SEM. (D) Percentage of surviving A549 normoxic and hypoxic cells 24 h post-treatment with Mb@MIL-100(Fe)-2 (60 μg/mL) and free Mb (6 μg/mL). Note that Mb@MIL-100(Fe)-2 biocomposite and MIL-100(Fe) are totally biocompatible at work concentration.
    Figure Legend Snippet: (A) Mechanism of action of the Mb@MIL-100(Fe) biocomposite in hypoxic cells. Cisplatin (CPT) treatment results in the survival of hypoxic A549 cells and apoptosis of normoxic A549 cells. Incubating hypoxic A549 cells with Mb@MIL-100(Fe) -2 induces the intracellular release of Mb protein and the subsequent enhancement of intracellular O 2 , prompting the apoptosis of hypoxic A549 cells. (B) Representative confocal images of normoxic A549 cells. Cells were incubated under each condition in DMEM +10% FBS in 20% O 2 at 37 °C for different time points, fixed with 4% PFA, and images were acquired by using a confocal microscope Olympus FV1000. (C) Quantification of at least 10 images of each condition. Data represent mean ± SEM. (D) Percentage of surviving A549 normoxic and hypoxic cells 24 h post-treatment with Mb@MIL-100(Fe)-2 (60 μg/mL) and free Mb (6 μg/mL). Note that Mb@MIL-100(Fe)-2 biocomposite and MIL-100(Fe) are totally biocompatible at work concentration.

    Techniques Used: Incubation, Microscopy, Concentration Assay

    Mb@MIL-100(Fe) promotes intracellular delivery of O 2 and enhances the susceptibility of hypoxic A549 cells to chemotherapy. (A) Fluorescence microscopy images of normoxic (up) and hypoxic (bottom) A549 cells without treatment (i,ii) and treated with Mb@MIL-100(Fe)-2 at 600 μg/mL (iii,iv), MIL-100(Fe) at 600 μg/mL (v,vi), or free Mb at 60 μg/mL (vii,viii), measured after exposure to the Image-iT IGHR at 0.75 μM for 4 h. Cells were incubated under each condition in DMEM +10% FBS in 20% O 2 (normoxia) or 1.5% O 2 and 5% CO 2 (hypoxia) at 37 °C for 4 h, fixed with 4% paraformaldehyde (PFA), and images were acquired by using a fluorescence microscope NIKON Eclipse TE-2000S (λ exc = 470–490 nm, λ em = 520–560 nm). (B) Quantification of the fluorescence emission intensity of fluorescence microscopy images shown in (A). (C) Percentage of surviving cells under normoxia (colorless) or hypoxia (grey) conditions after CPT treatment. Hypoxic cells were significantly chemoresistant compared to normoxic cells. (D) Percentage of surviving A549 hypoxic cells 24 h post-treatment with Mb@MIL-100(Fe)-2 (green), MIL-100(Fe) (yellow), and free Mb (pink), in the presence of CPT, as compared to the vehicle (colorless) and CPT (gray) controls. Data in B-D represent mean ± SD of n = 3 replicates, and statistical significance was calculated using one-way ANOVA; * p
    Figure Legend Snippet: Mb@MIL-100(Fe) promotes intracellular delivery of O 2 and enhances the susceptibility of hypoxic A549 cells to chemotherapy. (A) Fluorescence microscopy images of normoxic (up) and hypoxic (bottom) A549 cells without treatment (i,ii) and treated with Mb@MIL-100(Fe)-2 at 600 μg/mL (iii,iv), MIL-100(Fe) at 600 μg/mL (v,vi), or free Mb at 60 μg/mL (vii,viii), measured after exposure to the Image-iT IGHR at 0.75 μM for 4 h. Cells were incubated under each condition in DMEM +10% FBS in 20% O 2 (normoxia) or 1.5% O 2 and 5% CO 2 (hypoxia) at 37 °C for 4 h, fixed with 4% paraformaldehyde (PFA), and images were acquired by using a fluorescence microscope NIKON Eclipse TE-2000S (λ exc = 470–490 nm, λ em = 520–560 nm). (B) Quantification of the fluorescence emission intensity of fluorescence microscopy images shown in (A). (C) Percentage of surviving cells under normoxia (colorless) or hypoxia (grey) conditions after CPT treatment. Hypoxic cells were significantly chemoresistant compared to normoxic cells. (D) Percentage of surviving A549 hypoxic cells 24 h post-treatment with Mb@MIL-100(Fe)-2 (green), MIL-100(Fe) (yellow), and free Mb (pink), in the presence of CPT, as compared to the vehicle (colorless) and CPT (gray) controls. Data in B-D represent mean ± SD of n = 3 replicates, and statistical significance was calculated using one-way ANOVA; * p

    Techniques Used: Fluorescence, Microscopy, Incubation

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    ATCC dmem medium
    Treatment of PC12 cells with CPE antibodies inhibited cell survival. A) Immunoblot of CPE secreted into the media from cells with/without metabolic stress. PC12 cells were subjected to metabolic stress by replacing <t>DMEM</t> supplemented with 10% <t>FBS</t> (complete
    Dmem Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem medium/product/ATCC
    Average 99 stars, based on 166 article reviews
    Price from $9.99 to $1999.99
    dmem medium - by Bioz Stars, 2022-10
    99/100 stars
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    Image Search Results


    Treatment of PC12 cells with CPE antibodies inhibited cell survival. A) Immunoblot of CPE secreted into the media from cells with/without metabolic stress. PC12 cells were subjected to metabolic stress by replacing DMEM supplemented with 10% FBS (complete

    Journal: Cancer letters

    Article Title: Carboxypeptidase E promotes Cancer Cell Survival, but inhibits Migration and Invasion

    doi: 10.1016/j.canlet.2013.08.011

    Figure Lengend Snippet: Treatment of PC12 cells with CPE antibodies inhibited cell survival. A) Immunoblot of CPE secreted into the media from cells with/without metabolic stress. PC12 cells were subjected to metabolic stress by replacing DMEM supplemented with 10% FBS (complete

    Article Snippet: PC12 cells were seeded on 10 cm dishes and allowed to grow to 70 – 85% confluency in DMEM medium (ATCC, Manassas, VA) supplemented with 10% FBS and 5% horse serum.

    Techniques:

    Growth kinetics of CCO cells in DME medium (panel a ). Glucose (♦), lactate (■), glutamine (×) and ammonia (▲) concentration (panel b )

    Journal: Cytotechnology

    Article Title: Growth characteristics of channel catfish ovary cells--influence of glucose and glutamine

    doi: 10.1007/s10616-008-9171-y

    Figure Lengend Snippet: Growth kinetics of CCO cells in DME medium (panel a ). Glucose (♦), lactate (■), glutamine (×) and ammonia (▲) concentration (panel b )

    Article Snippet: The growth curve of CCO cells in standard DME medium during 8 days in culture is shown in Fig. a.

    Techniques: Concentration Assay

    Osteogenic characterization of D1 stem cells on tissue culture plastic. DNA content ( A ), alkaline phosphatase activity ( B ), and osteocalcin ( C ) of D1 cell lysates over time when cultured in tissue culture plastic in Dulbecco's modified Eagle medium alone

    Journal:

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation

    doi: 10.1089/ten.tea.2007.0411

    Figure Lengend Snippet: Osteogenic characterization of D1 stem cells on tissue culture plastic. DNA content ( A ), alkaline phosphatase activity ( B ), and osteocalcin ( C ) of D1 cell lysates over time when cultured in tissue culture plastic in Dulbecco's modified Eagle medium alone

    Article Snippet: A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions.

    Techniques: Activity Assay, Cell Culture, Modification

    Systematic search and screening results. Presentation based on the PRISMA statement. 68 Systematic search was conducted in August 2020. Qualitative analysis included all calculations in this review except meta-analysis and meta-regressions. One reason for exclusion per excluded article. DMEM, Dulbecco’s Modified Eagle’s Medium; Exp, experiments; U-87 MG, Uppsala-87 Malignant Glioma.

    Journal: BMJ Open Science

    Article Title: Meta-analysis on reporting practices as a source of heterogeneity in in vitro cancer research

    doi: 10.1136/bmjos-2021-100272

    Figure Lengend Snippet: Systematic search and screening results. Presentation based on the PRISMA statement. 68 Systematic search was conducted in August 2020. Qualitative analysis included all calculations in this review except meta-analysis and meta-regressions. One reason for exclusion per excluded article. DMEM, Dulbecco’s Modified Eagle’s Medium; Exp, experiments; U-87 MG, Uppsala-87 Malignant Glioma.

    Article Snippet: We also required that cell viability was measured by colorimetric assay or by cell counting, and that the authors used Dulbecco’s Modified Eagle Medium (DMEM) as the ATCC recommends using a modified Eagle medium for U-87 MG cell cultures and as a recent review identified DMEM as the most frequently used medium for this type of experiment.

    Techniques: Modification