Journal: bioRxiv

Article Title: ClearX9-Stem™: an efficient, affordable & sustainable stem cell culture medium for biotech and cell-cultivated meat industries

doi: 10.1101/2023.05.24.542098

Figure Lengend Snippet: ClearX9-Stem™ enhanced cells proliferation rate of SL-29 (Chicken Embryonic Fibroblast) cells at 72 hours. Cell Viability Assay was done by MTT on SL-29 cells. Briefly, cells (5 x104 cells/ml) were plated in 96 well plates and grown with 10% Fetal Bovine Serum (FBS) enriched containing standard (DMEM) or conditional (ATCC-Formulated DMEM (Cat. No. 30-2002) + 5% Tryptose Phosphate Broth + 5% Fetal Bovine Serum) complete medium or ClearX9-Stem™ for 72 hours. After the end of the stipulated time interval, MTT dye was added and incubated for 5 hours in a 37°C and 5% CO 2 humidified chamber. The formed crystals were dissolved in DMSO and OD was taken at 570nM. The data shown are the mean from three parallel experiments, and each experiment was done in triplicate. ***p<0.001.

Article Snippet: ClearX9-Stem™ showed a significantly (p<0.001) increased growth rate of SL-29 cells with features of good health and stress-free condition compared to 10% FBS-enriched traditional (DMEM) or 5% FBS with 5% TPB supplemented in ATCC-formulated DMEM, (Cat. No. 30-2002) cell culture medium cells ( and ).

Techniques: Viability Assay, Incubation

Journal: bioRxiv

Article Title: ClearX9-Stem™: an efficient, affordable & sustainable stem cell culture medium for biotech and cell-cultivated meat industries

doi: 10.1101/2023.05.24.542098

Figure Lengend Snippet: ClearX9-Stem™ increased the SL-29 (Chicken Embryonic Fibroblast) cells growth rate at 72 hours. 7 × 105 cells/ml cells were plated in a 35mm2 culture dish and grown in the presence of 10% Fetal Bovine Serum (FBS) enriched containing standard (DMEM) or conditional (ATCC-Formulated DMEM (Cat. No. 30-2002) + 5% Tryptose Phosphate Broth + 5% Fetal Bovine Serum) complete medium or ClearX9-Stem™ and cell morphology was visualized after 72 hours using a bright field microscope (Images were captured by Zeiss Primovert inverted microscope at 20X magnification

Article Snippet: ClearX9-Stem™ showed a significantly (p<0.001) increased growth rate of SL-29 cells with features of good health and stress-free condition compared to 10% FBS-enriched traditional (DMEM) or 5% FBS with 5% TPB supplemented in ATCC-formulated DMEM, (Cat. No. 30-2002) cell culture medium cells ( and ).

Techniques: Microscopy, Inverted Microscopy