3 methylphenol  (Millipore)


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  • 95
    Name:
    3 Methylphenol
    Description:

    Catalog Number:
    442391
    Price:
    None
    Applications:
    3-Methylphenol can be used in the preparation of 3-methylcyclohexanone via hydrogenation reaction in the presence of nickel catalyst. It can also be used in the synthesis of toluene, via hydrogenation in the presence of bimetallic nickel-iron catalyst. It can find applications as a solvent for the ultraviolet-visible light spectral studies of chemically synthesized soluble acrylic acid doped polyaniline.
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    Structured Review

    Millipore 3 methylphenol
    3 Methylphenol

    https://www.bioz.com/result/3 methylphenol/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 methylphenol - by Bioz Stars, 2020-09
    95/100 stars

    Images

    1) Product Images from "Structural requirements for voltage-dependent block of muscle sodium channels by phenol derivatives"

    Article Title: Structural requirements for voltage-dependent block of muscle sodium channels by phenol derivatives

    Journal: British Journal of Pharmacology

    doi: 10.1038/sj.bjp.0704024

    Structures of the phenol derivatives 3,5-dimethyl-4-chlorophenol, 2-methyl-4-chlorophenol, 4-chlorophenol and 3-methylphenol.
    Figure Legend Snippet: Structures of the phenol derivatives 3,5-dimethyl-4-chlorophenol, 2-methyl-4-chlorophenol, 4-chlorophenol and 3-methylphenol.

    Techniques Used:

    2) Product Images from "Substrate promiscuity of polyketide synthase enables production of tsetse fly attractants 3-ethylphenol and 3-propylphenol by engineering precursor supply in yeast"

    Article Title: Substrate promiscuity of polyketide synthase enables production of tsetse fly attractants 3-ethylphenol and 3-propylphenol by engineering precursor supply in yeast

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-66997-5

    Influence of overexpression of a propionyl-CoA synthetase on 3-ethylphenol ( A ) and 3-methylphenol ( B ) formation with and without supplementation of external propionate. Yeast strains CEN.PK2-1C expressing the 3-methylphenol pathway ( Ppopt MSAS , opt npgA and opt patG 14 ) and additionally the propionyl-CoA synthase opt prpE , with or without the Δcit2Δcit3 double deletion (strains JHY185 and JHY218, respectively), were inoculated at an OD of 5 and cultivated for 144 h in KP i buffered YPD medium (pH 6.5) with or without supplementation of 10 mM propionate. Culture supernatants were analysed via HPLC for 3-alkylphenol production. Error bars represent standard deviations of biological duplicates.
    Figure Legend Snippet: Influence of overexpression of a propionyl-CoA synthetase on 3-ethylphenol ( A ) and 3-methylphenol ( B ) formation with and without supplementation of external propionate. Yeast strains CEN.PK2-1C expressing the 3-methylphenol pathway ( Ppopt MSAS , opt npgA and opt patG 14 ) and additionally the propionyl-CoA synthase opt prpE , with or without the Δcit2Δcit3 double deletion (strains JHY185 and JHY218, respectively), were inoculated at an OD of 5 and cultivated for 144 h in KP i buffered YPD medium (pH 6.5) with or without supplementation of 10 mM propionate. Culture supernatants were analysed via HPLC for 3-alkylphenol production. Error bars represent standard deviations of biological duplicates.

    Techniques Used: Over Expression, Expressing, High Performance Liquid Chromatography

    3-Propylphenol formation via ‘reverse ß-oxidation’. 3-Propylphenol ( A ) and 3-methylphenol production ( B ) was measured in culture supernatants of CEN.PK2-1C expressing the 3-methylphenol pathway ( Ppopt MSAS , opt npgA and opt patG 14 ) with or without additional expression of the ‘reverse ß-oxidation’ pathway ( ERG10 , opt hbd , opt crt and opt ter 21 , 22 ) (strains 194 and 162, respectively), and with additional pox1 deletion (strains JHY212 and JHY211, respectively). High-OD fermentations (starting OD = 5) were performed in biological duplicates at 30 °C in KP i buffered YPD medium at pH 6.5. Culture supernatants were analysed via HPLC for 3-alkylphenol production. Error bars represent standard deviations.
    Figure Legend Snippet: 3-Propylphenol formation via ‘reverse ß-oxidation’. 3-Propylphenol ( A ) and 3-methylphenol production ( B ) was measured in culture supernatants of CEN.PK2-1C expressing the 3-methylphenol pathway ( Ppopt MSAS , opt npgA and opt patG 14 ) with or without additional expression of the ‘reverse ß-oxidation’ pathway ( ERG10 , opt hbd , opt crt and opt ter 21 , 22 ) (strains 194 and 162, respectively), and with additional pox1 deletion (strains JHY212 and JHY211, respectively). High-OD fermentations (starting OD = 5) were performed in biological duplicates at 30 °C in KP i buffered YPD medium at pH 6.5. Culture supernatants were analysed via HPLC for 3-alkylphenol production. Error bars represent standard deviations.

    Techniques Used: Expressing, High Performance Liquid Chromatography

    Metabolic pathways for 3-alkylphenol production in S. cerevisiae . In S. cerevisiae the heterologous polyketide synthase MSAS, activated by phosphopantetheinyl transferase (NpgA), catalyses the formation of 6-methylsalicylic acid (6-MSA) utilizing malonyl-CoA as extender unit and acetyl-CoA as priming unit. Intracellular propionyl-CoA can be increased by expression of a bacterial propionyl-CoA synthase (PrpE), propionate feeding and deletion of (methyl) citrate synthase genes CIT2/3 to abolish its degradation. MSAS can then utilize propionyl-CoA as priming unit to catalyse the formation of 6-ethylsalicylic acid (6-ESA). The heterologous ‘reverse ß-oxidation’ pathway 21 , 22 is providing the priming unit butyryl-CoA from acetyl-CoA for the formation of 6-propylsalicylic acid (6-PSA). Finally, 6-MSA decarboxylase (PatG) converts the 6-alkylsalicylic acids, 6-MSA, 6-ESA or 6-PSA, to their respective 3-alkylphenols (3-methylphenol, 3-ethylphenol or 3-propylphenol) that are valuable tsetse fly attractants.
    Figure Legend Snippet: Metabolic pathways for 3-alkylphenol production in S. cerevisiae . In S. cerevisiae the heterologous polyketide synthase MSAS, activated by phosphopantetheinyl transferase (NpgA), catalyses the formation of 6-methylsalicylic acid (6-MSA) utilizing malonyl-CoA as extender unit and acetyl-CoA as priming unit. Intracellular propionyl-CoA can be increased by expression of a bacterial propionyl-CoA synthase (PrpE), propionate feeding and deletion of (methyl) citrate synthase genes CIT2/3 to abolish its degradation. MSAS can then utilize propionyl-CoA as priming unit to catalyse the formation of 6-ethylsalicylic acid (6-ESA). The heterologous ‘reverse ß-oxidation’ pathway 21 , 22 is providing the priming unit butyryl-CoA from acetyl-CoA for the formation of 6-propylsalicylic acid (6-PSA). Finally, 6-MSA decarboxylase (PatG) converts the 6-alkylsalicylic acids, 6-MSA, 6-ESA or 6-PSA, to their respective 3-alkylphenols (3-methylphenol, 3-ethylphenol or 3-propylphenol) that are valuable tsetse fly attractants.

    Techniques Used: Expressing

    Effect of deletion of methylcitrate synthase genes CIT2 and CIT3 on 3-ethylphenol ( A ) and 3-methylphenol ( B ) formation with or without supplementation of external propionate and on propionate consumption ( C ). CEN.PK2-1C expressing the 3-methylphenol pathway (JHY162) ( Ppopt MSAS , opt npgA and opt patG 14 ) and with the Δcit2Δcit3 double deletion (JHY197) were utilized for high-OD fermentations (starting OD = 5.0) at 30 °C in KP i buffered YPD medium (pH 6.5) with or without supplementation of 10 mM propionate. Propionate consumption was followed in S. cerevisiae wild-type strain CEN.PK2-1C and deletion strains that either had peroxisomal ( Δcit2 ), mitochondrial ( Δcit3 ) or both methylcitrate synthases ( Δcit2/Δcit3 ) deleted and were cultured (starting OD = 4) at 30 °C in KP i buffered YPD medium (pH 6.5) supplemented with 10 mM propionate. Culture supernatants were analysed via HPLC for 3-alkylphenol production and propionate. Error bars represent standard deviations of biological duplicates.
    Figure Legend Snippet: Effect of deletion of methylcitrate synthase genes CIT2 and CIT3 on 3-ethylphenol ( A ) and 3-methylphenol ( B ) formation with or without supplementation of external propionate and on propionate consumption ( C ). CEN.PK2-1C expressing the 3-methylphenol pathway (JHY162) ( Ppopt MSAS , opt npgA and opt patG 14 ) and with the Δcit2Δcit3 double deletion (JHY197) were utilized for high-OD fermentations (starting OD = 5.0) at 30 °C in KP i buffered YPD medium (pH 6.5) with or without supplementation of 10 mM propionate. Propionate consumption was followed in S. cerevisiae wild-type strain CEN.PK2-1C and deletion strains that either had peroxisomal ( Δcit2 ), mitochondrial ( Δcit3 ) or both methylcitrate synthases ( Δcit2/Δcit3 ) deleted and were cultured (starting OD = 4) at 30 °C in KP i buffered YPD medium (pH 6.5) supplemented with 10 mM propionate. Culture supernatants were analysed via HPLC for 3-alkylphenol production and propionate. Error bars represent standard deviations of biological duplicates.

    Techniques Used: Expressing, Cell Culture, High Performance Liquid Chromatography

    Related Articles

    other:

    Article Title: Substrate promiscuity of polyketide synthase enables production of tsetse fly attractants 3-ethylphenol and 3-propylphenol by engineering precursor supply in yeast
    Article Snippet: 3-methylphenol and 3-ethylphenol were separated by the same gradient of solvent A (0.1% (v/v ) formic acid in ddH2 O) and solvent B (0.1% (v/v ) formic acid in acetonitrile) mentioned before .

    Article Title: Effects of Bunch Rot (Botrytis cinerea) and Powdery Mildew (Erysiphe necator) Fungal Diseases on Wine Aroma
    Article Snippet: The reference substances were obtained from the following suppliers: ethyl isobutanoate, ethyl 2-methylbutanoate, 3-methyl-1-butanol (isoamyl alcohol), ethyl hexanoate, (Z )-3-hexen-1-ol, (E )-2-octenal, acetic acid, 3-(methylthio)-propanal (methional), 3,7-dimethyl-1,6-octadien-3-ol (linalool), 3-methylbutanoicacid, 1,4-diethylbutanedionate (diethyl succinate), 3-(methylthio)-1-propanol (methionol), (E )-3,7-dimethyl-2,6-octadien-1-ol-acetate (geranylacetate), phenylacetate, hexanoic acid, benzyl alcohol, 2-phenylethanol, γ-nonalactone, 4-ethyl-2-methoxyphenol(4-ethylguaiacol), octanoic acid, 5(or 2)-ethyl-4-hydroxy-2(or 5)-methyl-3(2H )-furanone (homofuraneol), 3-methylphenol (m-cresol), γ-decalactone, 3-hydroxy-4,5-dimethyl-2(5H )-furanone (sotolone), 4-ethylphenol, 4-ethenyl-2-methoxyphenol (4-vinylguaiacol), decanoic acid, (4S ,4aS ,8aR )-4,8a-dimethyl-1,2,3,4,5,6,7,8-octahydronaphtalen-4a-ol [(±)-geosmin], γ-undecalactone and phenylacetic acid were purchased from Sigma-Aldrich (Steinheim, Germany).

    Synthesized:

    Article Title: Overcoming co-product inhibition in the nicotinamide independent asymmetric bioreduction of activated C=C-bonds using flavin-dependent ene-reductases
    Article Snippet: .. Cyclohex-2-enone ( 1a ), cyclohexanone ( 1b ), phenol ( 1d ), 4-ketoisophorone ( 2a ), N -phenyl-2-methylmaleimide ( 4a ), 1,4-dihydroxybenzene ( 5d ), 3-methylcyclohex-2-enone ( 6c ), and 3-methylphenol ( 6d ) were purchased from Sigma-Aldrich (St. Louis, MO), 1,4-cyclohexanedione ( 5c ) was from Fluka. rac -2,3-Epoxy-1-cyclohexanone ( 1e ) (Mueller et al., ), dimethyl citraconate ( 3a ), rac -dimethyl 2-methylsuccinate (rac - 3b ) (Stueckler et al., ), and rac -N -phenyl-2-methylsuccinimide (rac - 4b ) (Hall et al., ) were synthesized as previously reported. .. Levodione (rac - 2b ) was kindly provided by BASF-SE (Ludwigshafen).

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    Millipore src inhibitor pp1
    The <t>Src</t> inhibitor <t>PP1</t> enhances migration and attenuates ligand-dependent EGFR ubiquitylation. ( A ) Human telomerase-immortalized corneal epithelial cells were pretreated without or with 12 μM ( left ) or 0.01 μM ( right ) as indicated. Cells
    Src Inhibitor Pp1, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/src inhibitor pp1/product/Millipore
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
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    94/100 stars
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    93
    Millipore hsp70 inhibitors
    <t>HSP70</t> plays a role in mediating thermally-enhanced TNF-α production in macrophages. A , Peritoneal macrophages were isolated from LPS-challenged mice after 2 hour heat treatment. Cells were recovered overnight and re-stimulated (1×10 6 /well) in vitro with LPS and IFN-γ at 37°C for 4 hours, then HSP70 mRNA level was measured by quantitative real-time PCR. The results are presented relative to GAPDH and baseline expression in unstimulated cells from RT-mice. B , Macrophages (2×10 5 /well) from LPS-challenged mice were re-stimulated with LPS and IFN-γ at 37°C for 2, 6 or 24 hours to examine HSP70 secretion by ELISA. C , Macrophages (2×10 5 /well) from LPS-challenged mice were re-stimulated with LPS and IFN-γ at 37°C for 6 hours with or without HSP70 inhibitors: KNK437 (20, 10 µM) or Pifithrin-µ (5, 1 µM) to detect TNF-α production by ELISA. Cells from each treatment condition were pooled from 2–4 mice and measured in triplicate. Data are mean ± SD. Data are representative of two independent experiments. * In comparison of cells with and without HSP70 inhibitors from WBH-mice. ** In comparison of cells with and without HSP70 inhibitors from RT-mice. *, ** p
    Hsp70 Inhibitors, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore c57 bl6 mice
    Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive <t>C57/BL6</t> mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p
    C57 Bl6 Mice, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Src inhibitor PP1 enhances migration and attenuates ligand-dependent EGFR ubiquitylation. ( A ) Human telomerase-immortalized corneal epithelial cells were pretreated without or with 12 μM ( left ) or 0.01 μM ( right ) as indicated. Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Antagonizing c-Cbl Enhances EGFR-Dependent Corneal Epithelial Homeostasis

    doi: 10.1167/iovs.14-14133

    Figure Lengend Snippet: The Src inhibitor PP1 enhances migration and attenuates ligand-dependent EGFR ubiquitylation. ( A ) Human telomerase-immortalized corneal epithelial cells were pretreated without or with 12 μM ( left ) or 0.01 μM ( right ) as indicated. Cells

    Article Snippet: The Src inhibitor PP1 (4-amino-5-[4-methylphenyl]-7-[t-butyl]pyrazolo-d-3,4-pyrimidine) (EMD Millipore, Billerica, MA, USA) was solubilized in DMSO at a concentration of 50 mM.

    Techniques: Migration

    HSP70 plays a role in mediating thermally-enhanced TNF-α production in macrophages. A , Peritoneal macrophages were isolated from LPS-challenged mice after 2 hour heat treatment. Cells were recovered overnight and re-stimulated (1×10 6 /well) in vitro with LPS and IFN-γ at 37°C for 4 hours, then HSP70 mRNA level was measured by quantitative real-time PCR. The results are presented relative to GAPDH and baseline expression in unstimulated cells from RT-mice. B , Macrophages (2×10 5 /well) from LPS-challenged mice were re-stimulated with LPS and IFN-γ at 37°C for 2, 6 or 24 hours to examine HSP70 secretion by ELISA. C , Macrophages (2×10 5 /well) from LPS-challenged mice were re-stimulated with LPS and IFN-γ at 37°C for 6 hours with or without HSP70 inhibitors: KNK437 (20, 10 µM) or Pifithrin-µ (5, 1 µM) to detect TNF-α production by ELISA. Cells from each treatment condition were pooled from 2–4 mice and measured in triplicate. Data are mean ± SD. Data are representative of two independent experiments. * In comparison of cells with and without HSP70 inhibitors from WBH-mice. ** In comparison of cells with and without HSP70 inhibitors from RT-mice. *, ** p

    Journal: PLoS ONE

    Article Title: Elevation in Body Temperature to Fever Range Enhances and Prolongs Subsequent Responsiveness of Macrophages to Endotoxin Challenge

    doi: 10.1371/journal.pone.0030077

    Figure Lengend Snippet: HSP70 plays a role in mediating thermally-enhanced TNF-α production in macrophages. A , Peritoneal macrophages were isolated from LPS-challenged mice after 2 hour heat treatment. Cells were recovered overnight and re-stimulated (1×10 6 /well) in vitro with LPS and IFN-γ at 37°C for 4 hours, then HSP70 mRNA level was measured by quantitative real-time PCR. The results are presented relative to GAPDH and baseline expression in unstimulated cells from RT-mice. B , Macrophages (2×10 5 /well) from LPS-challenged mice were re-stimulated with LPS and IFN-γ at 37°C for 2, 6 or 24 hours to examine HSP70 secretion by ELISA. C , Macrophages (2×10 5 /well) from LPS-challenged mice were re-stimulated with LPS and IFN-γ at 37°C for 6 hours with or without HSP70 inhibitors: KNK437 (20, 10 µM) or Pifithrin-µ (5, 1 µM) to detect TNF-α production by ELISA. Cells from each treatment condition were pooled from 2–4 mice and measured in triplicate. Data are mean ± SD. Data are representative of two independent experiments. * In comparison of cells with and without HSP70 inhibitors from WBH-mice. ** In comparison of cells with and without HSP70 inhibitors from RT-mice. *, ** p

    Article Snippet: In some experiments, macrophages were treated with HSP70 inhibitors (KNK437, EMD chemicals; Pifithrin-µ, Sigma-Aldrich) together with 100 ng/mL LPS and 25 U IFN-γ.

    Techniques: Isolation, Mouse Assay, In Vitro, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay

    Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p

    Journal: Circulation

    Article Title: Cyclooxygenase-2, asymmetric dimethylarginine and the cardiovascular hazard from NSAIDs.

    doi: 10.1161/CIRCULATIONAHA.118.033540

    Figure Lengend Snippet: Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p

    Article Snippet: Ind.Cox-2 KO, WT and C57/BL6 mice (on a control diet or a celecoxib diet for three weeks) at 12–14 week of age received Ang II (1500 μg/Kg per day, Calbiochem, Darmstadt, Germany) in saline by continuous-infusion for 2 weeks.

    Techniques: Mouse Assay