3 diaminobenzidine (Millipore)

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Name:

3 3 Diaminobenzidine

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Catalog Number:

d8001

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None

Applications:

Peroxidase substrate; reagent for spectrophotometric determination of selenium.

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Structured Review

Millipore 3 diaminobenzidine
3 3 Diaminobenzidine

https://www.bioz.com/result/3 diaminobenzidine/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

3 diaminobenzidine - by Bioz Stars, 2021-03

99/100 stars

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Incubation:

Article Title: Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis
Article Snippet: After rinsing with 0.01 M PBS, the sections were incubated with Rabbit IgG SABC Kit (Boster, Wuhan, China, SA1022). .. Following a second rinsing with 0.01 M PBS, the reaction products were visualized by the addition of chromogenic substrate 0.1% diaminobenzidine solution (Sigma-Aldrich, St. Louis, MO, USA) in 0.1% hydrogen peroxide in PBS and incubated for 5 min. .. Subsequently the slides were lightly counterstained with Harris hematoxylin.

Activity Assay:

Article Title: La autoantigen is required for the internal ribosome entry site-mediated translation of Coxsackievirus B3 RNA
Article Snippet: The membranes were probed with horseradish peroxidase conjugated anti-rabbit secondary antibodies (Bangalore Genei). .. Peroxidase activity detection was carried out using Diaminobenzidine (Sigma-Aldrich) and 3% H2 O2 in PBS. .. A replica blot was probed with mouse anti-actin antibodies (Santa Cruz Biotech) and anti-mouse secondary antibodies followed by enhanced chemiluminescence (ECL) detection (Amersham-Pharmacia).

Avidin-Biotin Assay:

Article Title: Infiltration of forkhead box P3-expressing cells in small intestinal mucosa in coeliac disease but not in type 1 diabetes
Article Snippet: The antibodies were diluted in 1% normal horse sera and the final concentrations were as follows: FoxP3 (1:40) 10 μg/ml (clone 236 A/E7; Abcam, Cambridge, UK) and CD25 (1:10) 7·4 μg/ml (clone ACT-1; Dakocytomatin, Glostrup, Denmark). .. Bound antibody was detected with a commercial avidin–biotin immunoperoxidase system (Vectastain Elite ABC kit) according to the manufacturer's instructions, using 3,3′-diaminobenzidine chromogen (DAB; Sigma) as substrate. .. Double immunostaining for CD25 and FoxP3 was performed as described above, with the following modifications: after staining of CD25 antibody with DAB the slides were incubated with FoxP3 antibody over night at +4°C.

Staining:

Article Title: Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium-dependent endothelial nitric oxide synthase activity
Article Snippet: .. Staining using 3,3-diaminobenzidine (DAB) was initiated by adding freshly diluted 1 mg/ml DAB (Sigma-Aldrich; Merck KGaA) from a stock (10 mg/ml) dissolved in 0.1 M HCl and 10 mM H2 O2 in phosphate-buffered saline. ..

Western Blot:

Article Title: Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar Macrophages
Article Snippet: Treatment (16 h at 37°C) with N-glycosidase F (Boehringer Mannheim) in PBS without predenaturation using 4 U of enzyme was subsequently performed. .. Western blotting was carried out as recommended (Amersham) except that the detection step was performed using 3,3′diaminobenzidine (Sigma). .. Nucleotide and amino acid comparisons were performed using Blast (version 2; National Center for Biotechnology Information, National Institutes of Health, Bethesda, Md.) ( ).

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3 diaminobenzidine dab solutions (Millipore)

Millipore 3 diaminobenzidine dab solutions

Metabolic responses of wild-type and transgenic rice lines under cold stress. a ABA content ( n = 3, 32 plants/replication) and peroxidase (POX) enzyme activity ( n = 8, 10 plants/replication) of transgenic lines under cold stress. b The <t>3,3’-diaminobenzidine</t> (DAB) and c nitroblue tetrazolium (NBT) staining for the visualization of H 2 O 2 and O 2 − , respectively, in cold-treated rice leaves. Scale bars, 2 mm. d H 2 O 2 content ( n = 8, 10 plants/replication) of transgenic lines under cold stress. OE, overexpression; Ri, RNA interference; 73 Jap , bZIP73 Jap ; 73 Ind , bZIP73 Ind ; 71, bZIP71 ; 71–73 Jap OE, overexpression of both bZIP71 and bZIP73 Jap ; FW, fresh weight. All experiments were conducted using two-week-old seedlings. Error bar, standard deviation; ** p

3 Diaminobenzidine Dab Solutions, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 diaminobenzidine dab solutions/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

3 diaminobenzidine dab solutions - by Bioz Stars, 2021-03

99/100 stars

Buy from Supplier

Image Search Results

Journal: Nature Communications

Article Title: Early selection of bZIP73 facilitated adaptation of japonica rice to cold climates

doi: 10.1038/s41467-018-05753-w

Figure Lengend Snippet: Metabolic responses of wild-type and transgenic rice lines under cold stress. a ABA content ( n = 3, 32 plants/replication) and peroxidase (POX) enzyme activity ( n = 8, 10 plants/replication) of transgenic lines under cold stress. b The 3,3’-diaminobenzidine (DAB) and c nitroblue tetrazolium (NBT) staining for the visualization of H 2 O 2 and O 2 − , respectively, in cold-treated rice leaves. Scale bars, 2 mm. d H 2 O 2 content ( n = 8, 10 plants/replication) of transgenic lines under cold stress. OE, overexpression; Ri, RNA interference; 73 Jap , bZIP73 Jap ; 73 Ind , bZIP73 Ind ; 71, bZIP71 ; 71–73 Jap OE, overexpression of both bZIP71 and bZIP73 Jap ; FW, fresh weight. All experiments were conducted using two-week-old seedlings. Error bar, standard deviation; ** p

Article Snippet: The samples were infiltrated with nitroblue tetrazolium (NBT) (Amresco, USA; 1 mg mL−1 NBT in 10 mM sodium azide and 10 mM phosphate buffer, pH 7.8) or 3, 3’-diaminobenzidine (DAB) solutions (Sigma-Aldrich, USA; 1 mg mL−1 DAB-HCl, pH 3.8) .

Techniques: Transgenic Assay, Activity Assay, Staining, Over Expression, Standard Deviation

Carboxymethyl cellulose-coated carbon nanotubes (CMC-CNTs) stably bind to purified human properdin and recombinant TSR4+5, as shown via SDS-PAGE (A) and western blot (B) . (A) CMC-CNTs were incubated with recombinant human properdin, or thrombospondin type I repeat (TSR) 4 + 5-MBP fusion protein and BSA overnight in the affinity buffer. Carbon nanotubes (CNTs) were washed extensively via centrifugation and run on an SDS-PAGE (12%) under reduced conditions. Properdin migrated at ~55 kDa and MBP-TSR4+5 migrated as a single band at ~53 kDa. (B) In parallel, the SDS-PAGE was transferred onto nitrocellulose membrane for 2 h at 320 mA. The blot was probed with anti-human properdin (polyclonal) antibodies, followed by protein A–HRP conjugate and developed using 3,3′-diaminobenzidine.

Journal: Frontiers in Immunology

Article Title: Human Properdin Opsonizes Nanoparticles and Triggers a Potent Pro-inflammatory Response by Macrophages without Involving Complement Activation

doi: 10.3389/fimmu.2018.00131

Figure Lengend Snippet: Carboxymethyl cellulose-coated carbon nanotubes (CMC-CNTs) stably bind to purified human properdin and recombinant TSR4+5, as shown via SDS-PAGE (A) and western blot (B) . (A) CMC-CNTs were incubated with recombinant human properdin, or thrombospondin type I repeat (TSR) 4 + 5-MBP fusion protein and BSA overnight in the affinity buffer. Carbon nanotubes (CNTs) were washed extensively via centrifugation and run on an SDS-PAGE (12%) under reduced conditions. Properdin migrated at ~55 kDa and MBP-TSR4+5 migrated as a single band at ~53 kDa. (B) In parallel, the SDS-PAGE was transferred onto nitrocellulose membrane for 2 h at 320 mA. The blot was probed with anti-human properdin (polyclonal) antibodies, followed by protein A–HRP conjugate and developed using 3,3′-diaminobenzidine.

Article Snippet: Protein A–horseradish peroxidase (1:1,000; Thermo Scientific) in PBS was added and left at room temperature for 1 h. The blot was washed again with PBST three times, and the color was developed using 3,3′-diaminobenzidine (DAB) (Sigma-Aldrich).

Techniques: Stable Transfection, Purification, Recombinant, SDS Page, Western Blot, Incubation, Centrifugation

Wild-type p53-induced phosphatase 1 (Wip1) expression in normal lung and non small cell lung cancer (NSCLC) tissues using immunohistochemistry. (A) No Wip1 expression in normal lung tissue. (B) Weak (+1), (C) moderate (+2) and (D) strong (+3) Wip1 expression in NSCLC tissues. 3,3′-Diaminobenzidine and hematoxylin staining; magnification, x100.

Journal: Oncology Letters

Article Title: Association between overexpression of Wip1 and prognosis of patients with non-small cell lung cancer

doi: 10.3892/ol.2016.4245

Figure Lengend Snippet: Wild-type p53-induced phosphatase 1 (Wip1) expression in normal lung and non small cell lung cancer (NSCLC) tissues using immunohistochemistry. (A) No Wip1 expression in normal lung tissue. (B) Weak (+1), (C) moderate (+2) and (D) strong (+3) Wip1 expression in NSCLC tissues. 3,3′-Diaminobenzidine and hematoxylin staining; magnification, x100.

Article Snippet: 3,3′-Diaminobenzidine solution (Sigma-Aldrich) was used as a chromogen.

Techniques: Expressing, Immunohistochemistry, Staining

Porcine sialoadhesin immunoprecipitated from alveolar macrophages is modified with N-linked glycans. Alveolar macrophages were surface-labeled with biotin, and lysates were subjected to immunoprecipitation with MAb41D3. Immunoprecipitates were either treated with N -glycosidase F (lanes 2 and 3) or untreated (lanes 1 and 4) and run under reducing (lanes 1 and 2) or nonreducing (lanes 3 and 4) conditions on SDS-7% polyacrylamide gels. After transfer to polyvinylidene difluoride membrane, proteins on Western blots were detected with streptavidin-horseradish peroxidase, and this was followed by staining with 3,3′-diaminobenzidine as the substrate. M, apparent molecular mass markers (kilodaltons).

Journal: Journal of Virology

Article Title: Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar Macrophages

doi: 10.1128/JVI.77.15.8207-8215.2003

Figure Lengend Snippet: Porcine sialoadhesin immunoprecipitated from alveolar macrophages is modified with N-linked glycans. Alveolar macrophages were surface-labeled with biotin, and lysates were subjected to immunoprecipitation with MAb41D3. Immunoprecipitates were either treated with N -glycosidase F (lanes 2 and 3) or untreated (lanes 1 and 4) and run under reducing (lanes 1 and 2) or nonreducing (lanes 3 and 4) conditions on SDS-7% polyacrylamide gels. After transfer to polyvinylidene difluoride membrane, proteins on Western blots were detected with streptavidin-horseradish peroxidase, and this was followed by staining with 3,3′-diaminobenzidine as the substrate. M, apparent molecular mass markers (kilodaltons).

Article Snippet: Western blotting was carried out as recommended (Amersham) except that the detection step was performed using 3,3′diaminobenzidine (Sigma).

Techniques: Immunoprecipitation, Modification, Labeling, Western Blot, Staining