3 diaminobenzidine dab  (Millipore)


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  • 99
    Name:
    3 3 Diaminobenzidine DAB Liquid Substrate System tetrahydrochloride
    Description:

    Catalog Number:
    d7304
    Price:
    None
    Applications:
    DAB Liquid Substrate System is a precipitating substrate system for peroxidase. It produces a brown precipitate for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures. DAB has been used as a substrate in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver. It has been used as a substrate for visualization of sections after immunostaining.
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    Structured Review

    Millipore 3 diaminobenzidine dab
    Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by <t>3,3′-diaminobenzidine</t> (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    https://www.bioz.com/result/3 diaminobenzidine dab/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine dab - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield"

    Article Title: Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

    Journal: Rice

    doi: 10.1186/s12284-020-00396-2

    Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P
    Figure Legend Snippet: Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    Techniques Used: Blocking Assay, Staining, Microscopy, Expressing

    Osa-miR162a enhances the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and OX162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The above photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Relative mRNA levels of the defense-related genes ( OsKS4, OsNAC4, OsPR1 , and OsPR10b ) in NPB and OX162 following inoculation of M. oryzae GZ8. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P
    Figure Legend Snippet: Osa-miR162a enhances the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and OX162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The above photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Relative mRNA levels of the defense-related genes ( OsKS4, OsNAC4, OsPR1 , and OsPR10b ) in NPB and OX162 following inoculation of M. oryzae GZ8. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    Techniques Used: Staining, Microscopy

    Related Articles

    Staining:

    Article Title: Pentatricopeptide repeat protein DEK40 is required for mitochondrial function and kernel development in maize
    Article Snippet: .. For 3,3'-diaminobenzidine (DAB) staining to detect hydrogen peroxide (H2 O2 ), the kernels were cut longitudinally into two equal parts and placed in 1 mg ml−1 DAB (Sigma-Aldrich) in 50 mM Tris–HCl buffer (pH 5.0) in the dark for 12 h. For nitroblue tetrazolium chloride (NBT) staining to detect superoxides, the kernels were cut, immersed in a 0.5 mg ml−1 NBT (Sigma-Aldrich) solution in 10 mM potassium phosphate buffer (pH 7.6), and incubated in complete darkness at room temperature for 2 h. After each assay, the kernels were fixed in 70% (v/v) ethanol at room temperature. .. Photographs were taken using an Olympus DP72 microscope.

    Article Title: Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)
    Article Snippet: .. Cells were stained with 3,3'-diaminobenzidine (DAB) substrate solution by incubating cells in 200 μL premixed DAB solution (mix 30 μL (one drop) of the DAB liquid chromogen solution to 2 mL of the DAB liquid buffer solution (Sigma, # D 3939)) for 10 min. DAB solution was removed and cells rinsed briefly with PBS. .. Cells were counterstained with 20% Wright Giemsa solution for 1 min. Coverslips were mounted on microscope slides using a drop of permount mounting medium.

    Article Title: An Important Role for Syndecan-1 in Herpes Simplex Virus Type-1 Induced Cell-to-Cell Fusion and Virus Spread
    Article Snippet: .. Plaques were stained using rabbit anti-HSV-1 (Dako), horseradish peroxidase-conjugated secondary antibody (Amersham), and 3, 3′-diaminobenzidine tetrahydrochloride (DAB) substrate (Sigma). .. Plaques were measured with a micrometer at the 10× objective (Zeiss Axiovert 200), and the area was calculated by measuring the outline of each plaque using Axioversion software (version 4).

    Article Title: Dopaminergic Neurodegeneration in the Mouse Is Associated with Decrease of Viscoelasticity of Substantia Nigra Tissue
    Article Snippet: .. Then, ABC solution (Vectastain Elite ABC Kit, Vector Laboratories) was applied, before the formed streptavidin-peroxidase complex was visualized by 3,3'-diaminobenzidine (DAB, Sigma-Aldrich)-nickel staining. .. Finally, stained sections were mounted on microscope slides and coverslipped.

    Incubation:

    Article Title: Pentatricopeptide repeat protein DEK40 is required for mitochondrial function and kernel development in maize
    Article Snippet: .. For 3,3'-diaminobenzidine (DAB) staining to detect hydrogen peroxide (H2 O2 ), the kernels were cut longitudinally into two equal parts and placed in 1 mg ml−1 DAB (Sigma-Aldrich) in 50 mM Tris–HCl buffer (pH 5.0) in the dark for 12 h. For nitroblue tetrazolium chloride (NBT) staining to detect superoxides, the kernels were cut, immersed in a 0.5 mg ml−1 NBT (Sigma-Aldrich) solution in 10 mM potassium phosphate buffer (pH 7.6), and incubated in complete darkness at room temperature for 2 h. After each assay, the kernels were fixed in 70% (v/v) ethanol at room temperature. .. Photographs were taken using an Olympus DP72 microscope.

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    Millipore 3 3 diaminobenzidine dab
    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with <t>diaminobenzidine</t> <t>(DAB)</t> for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.
    3 3 Diaminobenzidine Dab, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    99
    Millipore 3 3 diaminobenzidine tetrahydrochloride
    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with <t>diaminobenzidine</t> <t>(DAB)</t> for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.
    3 3 Diaminobenzidine Tetrahydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 913 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride/product/Millipore
    Average 99 stars, based on 913 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Journal: Frontiers in Neuroanatomy

    Article Title: Putaminal Mosaic Visualized by Tyrosine Hydroxylase Immunohistochemistry in the Human Neostriatum

    doi: 10.3389/fnana.2016.00034

    Figure Lengend Snippet: Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Article Snippet: IHC with 3,3′-Diaminobenzidine (DAB) in Human Brain Tissue The sections were incubated for 18 h in 3% BSA-PBS containing a rabbit polyclonal antibody against TH (1:200,000; Sato et al., ) or [Met]-enkephalin (MEnk; 1:500,000; Millipore, St. Louis, MO, USA; Goto et al., ).

    Techniques: Staining, Marker

    Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Journal: Nature Communications

    Article Title: Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation

    doi: 10.1038/s41467-017-02033-x

    Figure Lengend Snippet: Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Article Snippet: Sections were rinsed with 0.1 M PBS (pH 7.4) for two, 5 min washes and then treated with 3% hydrogen peroxide for 10 min. We then used the 3,3′-Diaminobenzidine (DAB) IHC Select kit (DAB500, Millipore), but modified the company protocol (increased the duration of blocking, secondary antibody, streptavidin horseradish peroxidase, and DAB incubations to 10 min each).

    Techniques: Immunohistochemistry, Infection, Amplification