3 diaminobenzidine dab  (Millipore)

 
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    Name:
    3 3 Diaminobenzidine
    Description:

    Catalog Number:
    d8001
    Price:
    None
    Applications:
    Peroxidase substrate; reagent for spectrophotometric determination of selenium.
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    Structured Review

    Millipore 3 diaminobenzidine dab
    3 3 Diaminobenzidine

    https://www.bioz.com/result/3 diaminobenzidine dab/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine dab - by Bioz Stars, 2021-02
    99/100 stars

    Images

    1) Product Images from "Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield"

    Article Title: Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

    Journal: Rice

    doi: 10.1186/s12284-020-00396-2

    Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P
    Figure Legend Snippet: Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    Techniques Used: Blocking Assay, Staining, Microscopy, Expressing

    Osa-miR162a enhances the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and OX162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The above photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Relative mRNA levels of the defense-related genes ( OsKS4, OsNAC4, OsPR1 , and OsPR10b ) in NPB and OX162 following inoculation of M. oryzae GZ8. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P
    Figure Legend Snippet: Osa-miR162a enhances the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and OX162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The above photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Relative mRNA levels of the defense-related genes ( OsKS4, OsNAC4, OsPR1 , and OsPR10b ) in NPB and OX162 following inoculation of M. oryzae GZ8. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    Techniques Used: Staining, Microscopy

    Related Articles

    Staining:

    Article Title: Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium-dependent endothelial nitric oxide synthase activity
    Article Snippet: .. Staining using 3,3-diaminobenzidine (DAB) was initiated by adding freshly diluted 1 mg/ml DAB (Sigma-Aldrich; Merck KGaA) from a stock (10 mg/ml) dissolved in 0.1 M HCl and 10 mM H2 O2 in phosphate-buffered saline. ..

    Incubation:

    Article Title: Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis
    Article Snippet: .. Following a second rinsing with 0.01 M PBS, the reaction products were visualized by the addition of chromogenic substrate 0.1% diaminobenzidine solution (Sigma-Aldrich, St. Louis, MO, USA) in 0.1% hydrogen peroxide in PBS and incubated for 5 min. .. Subsequently the slides were lightly counterstained with Harris hematoxylin.

    Activity Assay:

    Article Title: La autoantigen is required for the internal ribosome entry site-mediated translation of Coxsackievirus B3 RNA
    Article Snippet: .. Peroxidase activity detection was carried out using Diaminobenzidine (Sigma-Aldrich) and 3% H2 O2 in PBS. .. A replica blot was probed with mouse anti-actin antibodies (Santa Cruz Biotech) and anti-mouse secondary antibodies followed by enhanced chemiluminescence (ECL) detection (Amersham-Pharmacia).

    Avidin-Biotin Assay:

    Article Title: Infiltration of forkhead box P3-expressing cells in small intestinal mucosa in coeliac disease but not in type 1 diabetes
    Article Snippet: .. Bound antibody was detected with a commercial avidin–biotin immunoperoxidase system (Vectastain Elite ABC kit) according to the manufacturer's instructions, using 3,3′-diaminobenzidine chromogen (DAB; Sigma) as substrate. .. Double immunostaining for CD25 and FoxP3 was performed as described above, with the following modifications: after staining of CD25 antibody with DAB the slides were incubated with FoxP3 antibody over night at +4°C.

    Western Blot:

    Article Title: Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar Macrophages
    Article Snippet: .. Western blotting was carried out as recommended (Amersham) except that the detection step was performed using 3,3′diaminobenzidine (Sigma). .. Nucleotide and amino acid comparisons were performed using Blast (version 2; National Center for Biotechnology Information, National Institutes of Health, Bethesda, Md.) ( ).

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    Millipore dab solution
    <t>NF200</t> <t>DAB</t> staining results ( a , b ) and 5HT immunohistochemical results ( c , d ) in the injured spinal cord tissue at 12 weeks after injury ( a – d ). ( a ) Representative images of NF200-positive axons in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 1–4) within the lesion cavities of contused spinal cords (outlined by black dashed lines). ( b ) The density of NF200-positive axons of the control and exercise groups ( n = 4 per group). ( c ) Representative images of 5HT-positive axons (green) and GFAP-positive astrocytes (red) in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 5–8) adjacent to the lesion cavities of contused spinal cords. ( d ) The density of 5HT-positive axons of the control and exercise groups ( n = 4 per group). * p
    Dab Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dab solution/product/Millipore
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    dab solution - by Bioz Stars, 2021-02
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    95
    Millipore 3 3 diaminobenzidine dab enhanced liquid substrate system tetrahydrochloride
    Primary roots of sorghum seedlings stained with <t>3,3’-diaminobenzidine</t> <t>(DAB).</t> Cross sections taken from a Si− (a-c) and a Si+ root (d-f) represent regions IV, III, and II of silica aggregation along the root, as defined in Figure 1 . Insets are enlargements of the red rectangles in each of the panels. Brown discoloration attributed to the precipitation of DAB can be seen in the endodermal ITCW and the cell walls of xylem elements in all three regions. The staining is more intense in region II of both treatments. This is the region of Si aggregation onset. Arrowheads indicate some silica aggregates. (g) A longitudinal view of DAB stained ITCW from a Si− root. Slightly stronger tainted spots (arrows) can be seen on the background of the stained cell wall. Pits are seen as dark dots. (h) A similar longitudinal view of a Si+ ITCW. The outlines of Si aggregates are evident in darker stain (arrows) on the uniformly stained ITCW. Scale bar in f, common to a-f represents 50 μm. Scale bar in h, common to g and h represents 5 μm.
    3 3 Diaminobenzidine Dab Enhanced Liquid Substrate System Tetrahydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab enhanced liquid substrate system tetrahydrochloride/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab enhanced liquid substrate system tetrahydrochloride - by Bioz Stars, 2021-02
    95/100 stars
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    Image Search Results


    NF200 DAB staining results ( a , b ) and 5HT immunohistochemical results ( c , d ) in the injured spinal cord tissue at 12 weeks after injury ( a – d ). ( a ) Representative images of NF200-positive axons in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 1–4) within the lesion cavities of contused spinal cords (outlined by black dashed lines). ( b ) The density of NF200-positive axons of the control and exercise groups ( n = 4 per group). ( c ) Representative images of 5HT-positive axons (green) and GFAP-positive astrocytes (red) in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 5–8) adjacent to the lesion cavities of contused spinal cords. ( d ) The density of 5HT-positive axons of the control and exercise groups ( n = 4 per group). * p

    Journal: Cells

    Article Title: Exercise Ameliorates Spinal Cord Injury by Changing DNA Methylation

    doi: 10.3390/cells10010143

    Figure Lengend Snippet: NF200 DAB staining results ( a , b ) and 5HT immunohistochemical results ( c , d ) in the injured spinal cord tissue at 12 weeks after injury ( a – d ). ( a ) Representative images of NF200-positive axons in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 1–4) within the lesion cavities of contused spinal cords (outlined by black dashed lines). ( b ) The density of NF200-positive axons of the control and exercise groups ( n = 4 per group). ( c ) Representative images of 5HT-positive axons (green) and GFAP-positive astrocytes (red) in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 5–8) adjacent to the lesion cavities of contused spinal cords. ( d ) The density of 5HT-positive axons of the control and exercise groups ( n = 4 per group). * p

    Article Snippet: Finally, NF200 staining was revealed with DAB solution (0.05% 3–3-diaminobenzidine tetrahydrochloride (MilliporeSigma), 0.06% NiCl2 (MilliporeSigma), and 0.003% H2 O2), and the reaction was stopped with distilled water.

    Techniques: Staining, Immunohistochemistry

    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Journal: Frontiers in Neuroanatomy

    Article Title: Putaminal Mosaic Visualized by Tyrosine Hydroxylase Immunohistochemistry in the Human Neostriatum

    doi: 10.3389/fnana.2016.00034

    Figure Lengend Snippet: Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Article Snippet: IHC with 3,3′-Diaminobenzidine (DAB) in Human Brain Tissue The sections were incubated for 18 h in 3% BSA-PBS containing a rabbit polyclonal antibody against TH (1:200,000; Sato et al., ) or [Met]-enkephalin (MEnk; 1:500,000; Millipore, St. Louis, MO, USA; Goto et al., ).

    Techniques: Staining, Marker

    Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Journal: Nature Communications

    Article Title: Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation

    doi: 10.1038/s41467-017-02033-x

    Figure Lengend Snippet: Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Article Snippet: Sections were rinsed with 0.1 M PBS (pH 7.4) for two, 5 min washes and then treated with 3% hydrogen peroxide for 10 min. We then used the 3,3′-Diaminobenzidine (DAB) IHC Select kit (DAB500, Millipore), but modified the company protocol (increased the duration of blocking, secondary antibody, streptavidin horseradish peroxidase, and DAB incubations to 10 min each).

    Techniques: Immunohistochemistry, Infection, Amplification

    Primary roots of sorghum seedlings stained with 3,3’-diaminobenzidine (DAB). Cross sections taken from a Si− (a-c) and a Si+ root (d-f) represent regions IV, III, and II of silica aggregation along the root, as defined in Figure 1 . Insets are enlargements of the red rectangles in each of the panels. Brown discoloration attributed to the precipitation of DAB can be seen in the endodermal ITCW and the cell walls of xylem elements in all three regions. The staining is more intense in region II of both treatments. This is the region of Si aggregation onset. Arrowheads indicate some silica aggregates. (g) A longitudinal view of DAB stained ITCW from a Si− root. Slightly stronger tainted spots (arrows) can be seen on the background of the stained cell wall. Pits are seen as dark dots. (h) A similar longitudinal view of a Si+ ITCW. The outlines of Si aggregates are evident in darker stain (arrows) on the uniformly stained ITCW. Scale bar in f, common to a-f represents 50 μm. Scale bar in h, common to g and h represents 5 μm.

    Journal: bioRxiv

    Article Title: Hydrogen peroxide modulates lignin and silica deposits in sorghum roots

    doi: 10.1101/2021.02.01.429181

    Figure Lengend Snippet: Primary roots of sorghum seedlings stained with 3,3’-diaminobenzidine (DAB). Cross sections taken from a Si− (a-c) and a Si+ root (d-f) represent regions IV, III, and II of silica aggregation along the root, as defined in Figure 1 . Insets are enlargements of the red rectangles in each of the panels. Brown discoloration attributed to the precipitation of DAB can be seen in the endodermal ITCW and the cell walls of xylem elements in all three regions. The staining is more intense in region II of both treatments. This is the region of Si aggregation onset. Arrowheads indicate some silica aggregates. (g) A longitudinal view of DAB stained ITCW from a Si− root. Slightly stronger tainted spots (arrows) can be seen on the background of the stained cell wall. Pits are seen as dark dots. (h) A similar longitudinal view of a Si+ ITCW. The outlines of Si aggregates are evident in darker stain (arrows) on the uniformly stained ITCW. Scale bar in f, common to a-f represents 50 μm. Scale bar in h, common to g and h represents 5 μm.

    Article Snippet: 3,3’-diaminobenzidine (DAB) staining and roots sectioningDAB staining was preformed according to ( ) with minor changes.

    Techniques: Staining