cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3
    Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved <t>caspase-3,</t> as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Vanillylacetone attenuates cadmium chloride-induced hippocampal damage and memory loss through up-regulation of nuclear factor erythroid 2-related factor 2 gene and protein expression"

    Article Title: Vanillylacetone attenuates cadmium chloride-induced hippocampal damage and memory loss through up-regulation of nuclear factor erythroid 2-related factor 2 gene and protein expression

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.391300

    Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved caspase-3, as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.
    Figure Legend Snippet: Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved caspase-3, as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.

    Techniques Used: Western Blot

    cleaved caspase 3 rabbit 9664s ab 10831820 cst  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3 rabbit 9664s ab 10831820 cst
    Cleaved Caspase 3 Rabbit 9664s Ab 10831820 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3
    SCI-induced apoptosis can be alleviated by treatment with Lup. (A) Nissl-stained neurons in the spinal cord. Neurons in the SCI group were darker and smaller than those in the sham group. The neurons in the SCI + Lup group were arranged more tightly and exhibited more intact morphology than those in the SCI group. Scale bar: 200 μm. (B) The number of Nissl bodies in the spinal cord. (C) TUNEL staining of spinal cord sections. Both the sham group and the sham + Lup group showed rare TUNEL-positive cells (red); the number of TUNEL-positive cells in the SCI group was markedly higher than that in the sham group; and the number of TUNEL-positive cells in the SCI + Lup group was markedly lower than that in the SCI group. Scale bar: 200 μm. (D) Quantification of TUNEL-positive cells. (E–H) Western blots and quantification of C-Cas3, Bcl-2, and Bax levels. Data (normalized by sham group) are expressed as mean ± SD ( n = 3). ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey's honest significant difference post hoc test). C-Cas3: Cleaved <t>caspase-3;</t> GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Lup: lupenone; NS: no significance; SCI: spinal cord injury; TUNEL: terminal deoxynucleotidyl transferase mediated dUTP nick end labeling.
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Lupenone improves motor dysfunction in spinal cord injury mice through inhibiting the inflammasome activation and pyroptosis in microglia via the nuclear factor kappa B pathway"

    Article Title: Lupenone improves motor dysfunction in spinal cord injury mice through inhibiting the inflammasome activation and pyroptosis in microglia via the nuclear factor kappa B pathway

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.389302

    SCI-induced apoptosis can be alleviated by treatment with Lup. (A) Nissl-stained neurons in the spinal cord. Neurons in the SCI group were darker and smaller than those in the sham group. The neurons in the SCI + Lup group were arranged more tightly and exhibited more intact morphology than those in the SCI group. Scale bar: 200 μm. (B) The number of Nissl bodies in the spinal cord. (C) TUNEL staining of spinal cord sections. Both the sham group and the sham + Lup group showed rare TUNEL-positive cells (red); the number of TUNEL-positive cells in the SCI group was markedly higher than that in the sham group; and the number of TUNEL-positive cells in the SCI + Lup group was markedly lower than that in the SCI group. Scale bar: 200 μm. (D) Quantification of TUNEL-positive cells. (E–H) Western blots and quantification of C-Cas3, Bcl-2, and Bax levels. Data (normalized by sham group) are expressed as mean ± SD ( n = 3). ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey's honest significant difference post hoc test). C-Cas3: Cleaved caspase-3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Lup: lupenone; NS: no significance; SCI: spinal cord injury; TUNEL: terminal deoxynucleotidyl transferase mediated dUTP nick end labeling.
    Figure Legend Snippet: SCI-induced apoptosis can be alleviated by treatment with Lup. (A) Nissl-stained neurons in the spinal cord. Neurons in the SCI group were darker and smaller than those in the sham group. The neurons in the SCI + Lup group were arranged more tightly and exhibited more intact morphology than those in the SCI group. Scale bar: 200 μm. (B) The number of Nissl bodies in the spinal cord. (C) TUNEL staining of spinal cord sections. Both the sham group and the sham + Lup group showed rare TUNEL-positive cells (red); the number of TUNEL-positive cells in the SCI group was markedly higher than that in the sham group; and the number of TUNEL-positive cells in the SCI + Lup group was markedly lower than that in the SCI group. Scale bar: 200 μm. (D) Quantification of TUNEL-positive cells. (E–H) Western blots and quantification of C-Cas3, Bcl-2, and Bax levels. Data (normalized by sham group) are expressed as mean ± SD ( n = 3). ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey's honest significant difference post hoc test). C-Cas3: Cleaved caspase-3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Lup: lupenone; NS: no significance; SCI: spinal cord injury; TUNEL: terminal deoxynucleotidyl transferase mediated dUTP nick end labeling.

    Techniques Used: Staining, TUNEL Assay, Western Blot, End Labeling

    cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 3 asp175 cell signaling wb  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3 asp175 cell signaling wb
    Cleaved Caspase 3 Asp175 Cell Signaling Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3
    A The Concentration of D-2HG in 14 IDH1-Mutant and 18 IDH1 Wild-Type Glioma Tissues. B The average concentration of D-2HG in IDH1 Wild-Type gliomas was 70.9 μM, whereas the average D-2HG concentration in IDH1-mutant gliomas was 1.9 mM, showing a statistically significant difference, P < 0.001. C IC 50 values of D-2HG in U251 cells at 24 h, 48 h, and 72 h were 1414 μM, 837.3 μM, and 869.3 μM, respectively. IC 50 of D-2HG was determined using the “log(inhibitor) vs. Normalized response -Variable slope” method in GraphPad Prism 9. D Cell viability. E Reduce total cell count. F The number of viable cells. G EdU assay was conducted to evaluate the DNA replication ability. H Quantification of EdU assay. I Cell apoptosis detected by flow cytometry. J Quantification of Cell apoptosis. K The cleaved <t>caspase-3</t> expression. L Death cell counts.
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "IDH1-mutant metabolite D-2-hydroxyglutarate inhibits proliferation and sensitizes glioma to temozolomide via down-regulating ITGB4/PI3K/AKT"

    Article Title: IDH1-mutant metabolite D-2-hydroxyglutarate inhibits proliferation and sensitizes glioma to temozolomide via down-regulating ITGB4/PI3K/AKT

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-024-02088-y

    A The Concentration of D-2HG in 14 IDH1-Mutant and 18 IDH1 Wild-Type Glioma Tissues. B The average concentration of D-2HG in IDH1 Wild-Type gliomas was 70.9 μM, whereas the average D-2HG concentration in IDH1-mutant gliomas was 1.9 mM, showing a statistically significant difference, P < 0.001. C IC 50 values of D-2HG in U251 cells at 24 h, 48 h, and 72 h were 1414 μM, 837.3 μM, and 869.3 μM, respectively. IC 50 of D-2HG was determined using the “log(inhibitor) vs. Normalized response -Variable slope” method in GraphPad Prism 9. D Cell viability. E Reduce total cell count. F The number of viable cells. G EdU assay was conducted to evaluate the DNA replication ability. H Quantification of EdU assay. I Cell apoptosis detected by flow cytometry. J Quantification of Cell apoptosis. K The cleaved caspase-3 expression. L Death cell counts.
    Figure Legend Snippet: A The Concentration of D-2HG in 14 IDH1-Mutant and 18 IDH1 Wild-Type Glioma Tissues. B The average concentration of D-2HG in IDH1 Wild-Type gliomas was 70.9 μM, whereas the average D-2HG concentration in IDH1-mutant gliomas was 1.9 mM, showing a statistically significant difference, P < 0.001. C IC 50 values of D-2HG in U251 cells at 24 h, 48 h, and 72 h were 1414 μM, 837.3 μM, and 869.3 μM, respectively. IC 50 of D-2HG was determined using the “log(inhibitor) vs. Normalized response -Variable slope” method in GraphPad Prism 9. D Cell viability. E Reduce total cell count. F The number of viable cells. G EdU assay was conducted to evaluate the DNA replication ability. H Quantification of EdU assay. I Cell apoptosis detected by flow cytometry. J Quantification of Cell apoptosis. K The cleaved caspase-3 expression. L Death cell counts.

    Techniques Used: Concentration Assay, Mutagenesis, Cell Counting, EdU Assay, Flow Cytometry, Expressing

    anti cleaved caspase 3 asp175  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cleaved caspase 3 asp175
    (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using <t>anti-cleaved</t> <t>Caspase-3</t> <t>(Asp175)</t> antibody. In the merged image: nuclei in cyan (DAPI); cleaved <t>Casp3</t> in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.
    Anti Cleaved Caspase 3 Asp175, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Memory of Somatic Cell Identity Affects Specific Cell Differentiation Programs Upon Reprogramming In Vivo"

    Article Title: Memory of Somatic Cell Identity Affects Specific Cell Differentiation Programs Upon Reprogramming In Vivo

    Journal: bioRxiv

    doi: 10.1101/2024.07.05.602198

    (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using anti-cleaved Caspase-3 (Asp175) antibody. In the merged image: nuclei in cyan (DAPI); cleaved Casp3 in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.
    Figure Legend Snippet: (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using anti-cleaved Caspase-3 (Asp175) antibody. In the merged image: nuclei in cyan (DAPI); cleaved Casp3 in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.

    Techniques Used: Staining, Immunohistochemistry, Immunofluorescence

    (A) Schematic of the microinjection experiment. The injected ventral blastomeres of 8-cell stage embryos are coloured in black. (B ) Images of representative embryos at stage 32 stained with anti-Tp63 immuno- histochemistry. Uninj.: uninjected, OE: injected embryos overexpressing Sox17b, Foxa4 and Kdm5b ci . Scale bar = 0.2 mm. (C) Proportions of embryos displaying an area with a depletion of Tp63+ basal stem cell (BSC) (white bar) across the different conditions. (D) Immunofluorescence staining of Tp63+ basal stem cells, α-ac-tubulin+ multiciliated cells and ZO-1+ cell borders in epidermis of uninjected embryos or embryos overexpressing Sox17b, Foxa4 and control proteins. In merged image: Tp63 in magenta, ZO-1 in green, α- ac-tubulin in blue. (E) Quantified proportions of epidermal cell types at stage 32 in each condition. The second chart from the top shows the proportions in regions lacking Tp63+ cells, and the third chart from the top shows epidermal cell proportions in regions with normal Tp63+ cell distribution in Sox17b OE embryos. Data represents mean values from Uninj, Sox17b OE Tp63 neg (n = 5), Sox17b OE Tp63 pos (n = 5), ctrl OE (n = 6). (F) Representative images of immunofluorescent staining for cleaved Caspase-3 and FLAG- Sox17b or FLAG-Foxa4 overexpressed proteins. Merged Image: Caspase 3 in magenta, DNA in green, FLAG tagged protein in blue. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. Uninj (n = 19), Sox17b OE (n = 10), Foxa4 OE (n = 16), ctrl OE (n = 7).).*p < 0.01, statistical test: Student’s unpaired t-test.
    Figure Legend Snippet: (A) Schematic of the microinjection experiment. The injected ventral blastomeres of 8-cell stage embryos are coloured in black. (B ) Images of representative embryos at stage 32 stained with anti-Tp63 immuno- histochemistry. Uninj.: uninjected, OE: injected embryos overexpressing Sox17b, Foxa4 and Kdm5b ci . Scale bar = 0.2 mm. (C) Proportions of embryos displaying an area with a depletion of Tp63+ basal stem cell (BSC) (white bar) across the different conditions. (D) Immunofluorescence staining of Tp63+ basal stem cells, α-ac-tubulin+ multiciliated cells and ZO-1+ cell borders in epidermis of uninjected embryos or embryos overexpressing Sox17b, Foxa4 and control proteins. In merged image: Tp63 in magenta, ZO-1 in green, α- ac-tubulin in blue. (E) Quantified proportions of epidermal cell types at stage 32 in each condition. The second chart from the top shows the proportions in regions lacking Tp63+ cells, and the third chart from the top shows epidermal cell proportions in regions with normal Tp63+ cell distribution in Sox17b OE embryos. Data represents mean values from Uninj, Sox17b OE Tp63 neg (n = 5), Sox17b OE Tp63 pos (n = 5), ctrl OE (n = 6). (F) Representative images of immunofluorescent staining for cleaved Caspase-3 and FLAG- Sox17b or FLAG-Foxa4 overexpressed proteins. Merged Image: Caspase 3 in magenta, DNA in green, FLAG tagged protein in blue. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. Uninj (n = 19), Sox17b OE (n = 10), Foxa4 OE (n = 16), ctrl OE (n = 7).).*p < 0.01, statistical test: Student’s unpaired t-test.

    Techniques Used: Microinjection, Injection, Staining, Immunohistochemistry, Immunofluorescence, Control

    (A) Schematic of the rescue experiment where NT-embryos were injected with antisense morpholinos (MO) into ventral blastomeres (in black) at 8-cell stage. (B) Immunohistochemistry for Tp63 protein at tailbud stage embryos in different conditions indicated at the top. Scale bar = 0.1 mm. Ctrl - control, MO - antisense morpholino. (C) Proportions of embryos showing perturbations in the composition of Tp63+ cells in the epidermis in the three conditions indicated on the vertical axis. (D) Immunofluorescence for activated Caspase-3 (Magenta), DAPI (Cyan) and fluorescent dextran (Green) in IVF, NT and NT embryos injected with sox17b morpholino. (E) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. IVF, NT Ctrl, NT sox17b MO.).*p < 0.01, statistical test: Student’s unpaired t-test.
    Figure Legend Snippet: (A) Schematic of the rescue experiment where NT-embryos were injected with antisense morpholinos (MO) into ventral blastomeres (in black) at 8-cell stage. (B) Immunohistochemistry for Tp63 protein at tailbud stage embryos in different conditions indicated at the top. Scale bar = 0.1 mm. Ctrl - control, MO - antisense morpholino. (C) Proportions of embryos showing perturbations in the composition of Tp63+ cells in the epidermis in the three conditions indicated on the vertical axis. (D) Immunofluorescence for activated Caspase-3 (Magenta), DAPI (Cyan) and fluorescent dextran (Green) in IVF, NT and NT embryos injected with sox17b morpholino. (E) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. IVF, NT Ctrl, NT sox17b MO.).*p < 0.01, statistical test: Student’s unpaired t-test.

    Techniques Used: Injection, Immunohistochemistry, Control, Immunofluorescence

    anti cleaved caspase 3 asp175  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cleaved caspase 3 asp175
    (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using <t>anti-cleaved</t> <t>Caspase-3</t> <t>(Asp175)</t> antibody. In the merged image: nuclei in cyan (DAPI); cleaved <t>Casp3</t> in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.
    Anti Cleaved Caspase 3 Asp175, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Memory of Somatic Cell Identity Affects Specific Cell Differentiation Programs Upon Reprogramming In Vivo"

    Article Title: Memory of Somatic Cell Identity Affects Specific Cell Differentiation Programs Upon Reprogramming In Vivo

    Journal: bioRxiv

    doi: 10.1101/2024.07.05.602198

    (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using anti-cleaved Caspase-3 (Asp175) antibody. In the merged image: nuclei in cyan (DAPI); cleaved Casp3 in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.
    Figure Legend Snippet: (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using anti-cleaved Caspase-3 (Asp175) antibody. In the merged image: nuclei in cyan (DAPI); cleaved Casp3 in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.

    Techniques Used: Staining, Immunohistochemistry, Immunofluorescence

    (A) Schematic of the microinjection experiment. The injected ventral blastomeres of 8-cell stage embryos are coloured in black. (B ) Images of representative embryos at stage 32 stained with anti-Tp63 immuno- histochemistry. Uninj.: uninjected, OE: injected embryos overexpressing Sox17b, Foxa4 and Kdm5b ci . Scale bar = 0.2 mm. (C) Proportions of embryos displaying an area with a depletion of Tp63+ basal stem cell (BSC) (white bar) across the different conditions. (D) Immunofluorescence staining of Tp63+ basal stem cells, α-ac-tubulin+ multiciliated cells and ZO-1+ cell borders in epidermis of uninjected embryos or embryos overexpressing Sox17b, Foxa4 and control proteins. In merged image: Tp63 in magenta, ZO-1 in green, α- ac-tubulin in blue. (E) Quantified proportions of epidermal cell types at stage 32 in each condition. The second chart from the top shows the proportions in regions lacking Tp63+ cells, and the third chart from the top shows epidermal cell proportions in regions with normal Tp63+ cell distribution in Sox17b OE embryos. Data represents mean values from Uninj, Sox17b OE Tp63 neg (n = 5), Sox17b OE Tp63 pos (n = 5), ctrl OE (n = 6). (F) Representative images of immunofluorescent staining for cleaved Caspase-3 and FLAG- Sox17b or FLAG-Foxa4 overexpressed proteins. Merged Image: Caspase 3 in magenta, DNA in green, FLAG tagged protein in blue. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. Uninj (n = 19), Sox17b OE (n = 10), Foxa4 OE (n = 16), ctrl OE (n = 7).).*p < 0.01, statistical test: Student’s unpaired t-test.
    Figure Legend Snippet: (A) Schematic of the microinjection experiment. The injected ventral blastomeres of 8-cell stage embryos are coloured in black. (B ) Images of representative embryos at stage 32 stained with anti-Tp63 immuno- histochemistry. Uninj.: uninjected, OE: injected embryos overexpressing Sox17b, Foxa4 and Kdm5b ci . Scale bar = 0.2 mm. (C) Proportions of embryos displaying an area with a depletion of Tp63+ basal stem cell (BSC) (white bar) across the different conditions. (D) Immunofluorescence staining of Tp63+ basal stem cells, α-ac-tubulin+ multiciliated cells and ZO-1+ cell borders in epidermis of uninjected embryos or embryos overexpressing Sox17b, Foxa4 and control proteins. In merged image: Tp63 in magenta, ZO-1 in green, α- ac-tubulin in blue. (E) Quantified proportions of epidermal cell types at stage 32 in each condition. The second chart from the top shows the proportions in regions lacking Tp63+ cells, and the third chart from the top shows epidermal cell proportions in regions with normal Tp63+ cell distribution in Sox17b OE embryos. Data represents mean values from Uninj, Sox17b OE Tp63 neg (n = 5), Sox17b OE Tp63 pos (n = 5), ctrl OE (n = 6). (F) Representative images of immunofluorescent staining for cleaved Caspase-3 and FLAG- Sox17b or FLAG-Foxa4 overexpressed proteins. Merged Image: Caspase 3 in magenta, DNA in green, FLAG tagged protein in blue. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. Uninj (n = 19), Sox17b OE (n = 10), Foxa4 OE (n = 16), ctrl OE (n = 7).).*p < 0.01, statistical test: Student’s unpaired t-test.

    Techniques Used: Microinjection, Injection, Staining, Immunohistochemistry, Immunofluorescence, Control

    (A) Schematic of the rescue experiment where NT-embryos were injected with antisense morpholinos (MO) into ventral blastomeres (in black) at 8-cell stage. (B) Immunohistochemistry for Tp63 protein at tailbud stage embryos in different conditions indicated at the top. Scale bar = 0.1 mm. Ctrl - control, MO - antisense morpholino. (C) Proportions of embryos showing perturbations in the composition of Tp63+ cells in the epidermis in the three conditions indicated on the vertical axis. (D) Immunofluorescence for activated Caspase-3 (Magenta), DAPI (Cyan) and fluorescent dextran (Green) in IVF, NT and NT embryos injected with sox17b morpholino. (E) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. IVF, NT Ctrl, NT sox17b MO.).*p < 0.01, statistical test: Student’s unpaired t-test.
    Figure Legend Snippet: (A) Schematic of the rescue experiment where NT-embryos were injected with antisense morpholinos (MO) into ventral blastomeres (in black) at 8-cell stage. (B) Immunohistochemistry for Tp63 protein at tailbud stage embryos in different conditions indicated at the top. Scale bar = 0.1 mm. Ctrl - control, MO - antisense morpholino. (C) Proportions of embryos showing perturbations in the composition of Tp63+ cells in the epidermis in the three conditions indicated on the vertical axis. (D) Immunofluorescence for activated Caspase-3 (Magenta), DAPI (Cyan) and fluorescent dextran (Green) in IVF, NT and NT embryos injected with sox17b morpholino. (E) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. IVF, NT Ctrl, NT sox17b MO.).*p < 0.01, statistical test: Student’s unpaired t-test.

    Techniques Used: Injection, Immunohistochemistry, Control, Immunofluorescence

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    Cell Signaling Technology Inc cleaved caspase 3
    Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved <t>caspase-3,</t> as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc cleaved caspase 3 rabbit 9664s ab 10831820 cst
    Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved <t>caspase-3,</t> as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.
    Cleaved Caspase 3 Rabbit 9664s Ab 10831820 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3 rabbit 9664s ab 10831820 cst/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc cleaved caspase 3 asp175 cell signaling wb
    Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved <t>caspase-3,</t> as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.
    Cleaved Caspase 3 Asp175 Cell Signaling Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3 asp175 cell signaling wb/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti cleaved caspase 3 asp175
    (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using <t>anti-cleaved</t> <t>Caspase-3</t> <t>(Asp175)</t> antibody. In the merged image: nuclei in cyan (DAPI); cleaved <t>Casp3</t> in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.
    Anti Cleaved Caspase 3 Asp175, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3 asp175/product/Cell Signaling Technology Inc
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    Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved caspase-3, as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.

    Journal: Neural Regeneration Research

    Article Title: Vanillylacetone attenuates cadmium chloride-induced hippocampal damage and memory loss through up-regulation of nuclear factor erythroid 2-related factor 2 gene and protein expression

    doi: 10.4103/1673-5374.391300

    Figure Lengend Snippet: Vanillylacetone suppresses intrinsic cell apoptosis in the hippocampi of CdCl 2 -treated rats in an Nrf2-dependent manner. (A and B) The mRNA levels of Bax and Bcl2, as quantified by qPCR. (C) The mRNA ratio of Bax/Bcl2 and (D) the protein levels of cleaved caspase-3, as measured by western blotting, in the hippocampi of all groups of rats. * P < 0.05, vs . control rats (lane I); # P < 0.05, vs . vanillylacetone (zingerone)-treated group (lane II); † P < 0.05, vs . CdCl 2 -treated rats (lane III); ‡ P < 0.05, vs . CdCl 2 + vanillylacetone-treated rats (lane IV). Lane V: CdCl 2 + vanillylacetone + brusatol. All data are presented as the mean (±SD) of three independent experiments. CdCl 2 : Cadmium chloride.

    Article Snippet: The membranes were then incubated for 2 hours at room temperature (23 ± 2°C) with primary antibodies against Nrf2 (61 kDa, 1:1000, Cat# 365949, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and cleaved caspase-3 (Cat# 9661, 17/19 kDa, 1:500, Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Western Blot

    (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using anti-cleaved Caspase-3 (Asp175) antibody. In the merged image: nuclei in cyan (DAPI); cleaved Casp3 in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.

    Journal: bioRxiv

    Article Title: Memory of Somatic Cell Identity Affects Specific Cell Differentiation Programs Upon Reprogramming In Vivo

    doi: 10.1101/2024.07.05.602198

    Figure Lengend Snippet: (A) Schematic representation of cell type-specific morphogenesis and cell maturation in mucociliary epidermis of Xenopus embryos. (B) Representative images of IVF- and NT-embryos at tailbud stage stained by whole mount immuno-histochemistry using monoclonal antibody anti-Tp63 protein; scale bar = 1 mm. (C) Bar plot showing proportions of NT- and IVF-embryos that experienced loss of Tp63 positive basal stem cells (BSC) in their epidermis. (D) Immunofluorescence staining of Tp63 positive basal stem cells and α-ac-tubulin positive multiciliated cells in epidermis of tailbud stage NT- and IVF-embryos. Antibody anti-ZO-1 (tight junction protein) was used for the detection of cell borders. (E) Quantified proportions of epidermal cell types in IVF- and NT-embryos at stage 32. The middle chart represents proportions in regions lacking Tp63+ cells. Data represents mean values from IVF (n = 7), NT Tp63 negative area (n = 5) and NT Tp63 positive area (n = 5). (F) Representative images from immunofluorescence of representative NT- and IVF- embryos at tailbud stage 32 using anti-cleaved Caspase-3 (Asp175) antibody. In the merged image: nuclei in cyan (DAPI); cleaved Casp3 in red. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. NT (n = 10) and IVF (n = 15).*p < 0.01, statistical test: Student’s unpaired t-test.

    Article Snippet: Embryos were blocked 1 hr in antibody buffer at RT and incubated depending on the experiment with following primary antibodies: Anti- Acetylated Tubulin (1:1000, Sigma-Aldrich, #T6793); anti ZO-1 (1:200, Invitrogen, #61-7300) or β-catenin (1:5, rat, hybridoma supernatant gifted from prof. Ralph Rupp); anti-cleaved Caspase-3 (Asp175) (1:400, CST, #9661).

    Techniques: Staining, Immunohistochemistry, Immunofluorescence

    (A) Schematic of the microinjection experiment. The injected ventral blastomeres of 8-cell stage embryos are coloured in black. (B ) Images of representative embryos at stage 32 stained with anti-Tp63 immuno- histochemistry. Uninj.: uninjected, OE: injected embryos overexpressing Sox17b, Foxa4 and Kdm5b ci . Scale bar = 0.2 mm. (C) Proportions of embryos displaying an area with a depletion of Tp63+ basal stem cell (BSC) (white bar) across the different conditions. (D) Immunofluorescence staining of Tp63+ basal stem cells, α-ac-tubulin+ multiciliated cells and ZO-1+ cell borders in epidermis of uninjected embryos or embryos overexpressing Sox17b, Foxa4 and control proteins. In merged image: Tp63 in magenta, ZO-1 in green, α- ac-tubulin in blue. (E) Quantified proportions of epidermal cell types at stage 32 in each condition. The second chart from the top shows the proportions in regions lacking Tp63+ cells, and the third chart from the top shows epidermal cell proportions in regions with normal Tp63+ cell distribution in Sox17b OE embryos. Data represents mean values from Uninj, Sox17b OE Tp63 neg (n = 5), Sox17b OE Tp63 pos (n = 5), ctrl OE (n = 6). (F) Representative images of immunofluorescent staining for cleaved Caspase-3 and FLAG- Sox17b or FLAG-Foxa4 overexpressed proteins. Merged Image: Caspase 3 in magenta, DNA in green, FLAG tagged protein in blue. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. Uninj (n = 19), Sox17b OE (n = 10), Foxa4 OE (n = 16), ctrl OE (n = 7).).*p < 0.01, statistical test: Student’s unpaired t-test.

    Journal: bioRxiv

    Article Title: Memory of Somatic Cell Identity Affects Specific Cell Differentiation Programs Upon Reprogramming In Vivo

    doi: 10.1101/2024.07.05.602198

    Figure Lengend Snippet: (A) Schematic of the microinjection experiment. The injected ventral blastomeres of 8-cell stage embryos are coloured in black. (B ) Images of representative embryos at stage 32 stained with anti-Tp63 immuno- histochemistry. Uninj.: uninjected, OE: injected embryos overexpressing Sox17b, Foxa4 and Kdm5b ci . Scale bar = 0.2 mm. (C) Proportions of embryos displaying an area with a depletion of Tp63+ basal stem cell (BSC) (white bar) across the different conditions. (D) Immunofluorescence staining of Tp63+ basal stem cells, α-ac-tubulin+ multiciliated cells and ZO-1+ cell borders in epidermis of uninjected embryos or embryos overexpressing Sox17b, Foxa4 and control proteins. In merged image: Tp63 in magenta, ZO-1 in green, α- ac-tubulin in blue. (E) Quantified proportions of epidermal cell types at stage 32 in each condition. The second chart from the top shows the proportions in regions lacking Tp63+ cells, and the third chart from the top shows epidermal cell proportions in regions with normal Tp63+ cell distribution in Sox17b OE embryos. Data represents mean values from Uninj, Sox17b OE Tp63 neg (n = 5), Sox17b OE Tp63 pos (n = 5), ctrl OE (n = 6). (F) Representative images of immunofluorescent staining for cleaved Caspase-3 and FLAG- Sox17b or FLAG-Foxa4 overexpressed proteins. Merged Image: Caspase 3 in magenta, DNA in green, FLAG tagged protein in blue. (G) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. Uninj (n = 19), Sox17b OE (n = 10), Foxa4 OE (n = 16), ctrl OE (n = 7).).*p < 0.01, statistical test: Student’s unpaired t-test.

    Article Snippet: Embryos were blocked 1 hr in antibody buffer at RT and incubated depending on the experiment with following primary antibodies: Anti- Acetylated Tubulin (1:1000, Sigma-Aldrich, #T6793); anti ZO-1 (1:200, Invitrogen, #61-7300) or β-catenin (1:5, rat, hybridoma supernatant gifted from prof. Ralph Rupp); anti-cleaved Caspase-3 (Asp175) (1:400, CST, #9661).

    Techniques: Microinjection, Injection, Staining, Immunohistochemistry, Immunofluorescence, Control

    (A) Schematic of the rescue experiment where NT-embryos were injected with antisense morpholinos (MO) into ventral blastomeres (in black) at 8-cell stage. (B) Immunohistochemistry for Tp63 protein at tailbud stage embryos in different conditions indicated at the top. Scale bar = 0.1 mm. Ctrl - control, MO - antisense morpholino. (C) Proportions of embryos showing perturbations in the composition of Tp63+ cells in the epidermis in the three conditions indicated on the vertical axis. (D) Immunofluorescence for activated Caspase-3 (Magenta), DAPI (Cyan) and fluorescent dextran (Green) in IVF, NT and NT embryos injected with sox17b morpholino. (E) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. IVF, NT Ctrl, NT sox17b MO.).*p < 0.01, statistical test: Student’s unpaired t-test.

    Journal: bioRxiv

    Article Title: Memory of Somatic Cell Identity Affects Specific Cell Differentiation Programs Upon Reprogramming In Vivo

    doi: 10.1101/2024.07.05.602198

    Figure Lengend Snippet: (A) Schematic of the rescue experiment where NT-embryos were injected with antisense morpholinos (MO) into ventral blastomeres (in black) at 8-cell stage. (B) Immunohistochemistry for Tp63 protein at tailbud stage embryos in different conditions indicated at the top. Scale bar = 0.1 mm. Ctrl - control, MO - antisense morpholino. (C) Proportions of embryos showing perturbations in the composition of Tp63+ cells in the epidermis in the three conditions indicated on the vertical axis. (D) Immunofluorescence for activated Caspase-3 (Magenta), DAPI (Cyan) and fluorescent dextran (Green) in IVF, NT and NT embryos injected with sox17b morpholino. (E) Quantification of cells positive for activated (cleaved) Caspase-3 per lateral side of stage 32 embryo. IVF, NT Ctrl, NT sox17b MO.).*p < 0.01, statistical test: Student’s unpaired t-test.

    Article Snippet: Embryos were blocked 1 hr in antibody buffer at RT and incubated depending on the experiment with following primary antibodies: Anti- Acetylated Tubulin (1:1000, Sigma-Aldrich, #T6793); anti ZO-1 (1:200, Invitrogen, #61-7300) or β-catenin (1:5, rat, hybridoma supernatant gifted from prof. Ralph Rupp); anti-cleaved Caspase-3 (Asp175) (1:400, CST, #9661).

    Techniques: Injection, Immunohistochemistry, Control, Immunofluorescence