anti nf 200  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti nf 200
    The expression of MBP and <t>NF-200</t> were detected by immunocytochemistry: ( A ) In experimental groups (defined in figure caption 1) the fluorescence intensity of MBP (green) and NF-200 (red) was elevated which was detected by immunocytochemistry. ( B, C ) the fluorescence intensity was measured by integrated density pixel. * p
    Anti Nf 200, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 29934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nf 200/product/Thermo Fisher
    Average 92 stars, based on 29934 article reviews
    Price from $9.99 to $1999.99
    anti nf 200 - by Bioz Stars, 2021-01
    92/100 stars

    Images

    1) Product Images from "Metformin Enhances Functional Recovery of Peripheral Nerve in Rats with Sciatic Nerve Crush Injury"

    Article Title: Metformin Enhances Functional Recovery of Peripheral Nerve in Rats with Sciatic Nerve Crush Injury

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.918277

    The expression of MBP and NF-200 were detected by immunocytochemistry: ( A ) In experimental groups (defined in figure caption 1) the fluorescence intensity of MBP (green) and NF-200 (red) was elevated which was detected by immunocytochemistry. ( B, C ) the fluorescence intensity was measured by integrated density pixel. * p
    Figure Legend Snippet: The expression of MBP and NF-200 were detected by immunocytochemistry: ( A ) In experimental groups (defined in figure caption 1) the fluorescence intensity of MBP (green) and NF-200 (red) was elevated which was detected by immunocytochemistry. ( B, C ) the fluorescence intensity was measured by integrated density pixel. * p

    Techniques Used: Expressing, Immunocytochemistry, Fluorescence

    2) Product Images from "Development of Folic Acid-Conjugated and Methylene Blue-Adsorbed Au@TNA Nanoparticles for Enhanced Photodynamic Therapy of Bladder Cancer Cells"

    Article Title: Development of Folic Acid-Conjugated and Methylene Blue-Adsorbed Au@TNA Nanoparticles for Enhanced Photodynamic Therapy of Bladder Cancer Cells

    Journal: Nanomaterials

    doi: 10.3390/nano10071351

    ( a ) Thiazolyl blue tetrazolium bromide (MTT) assay of HeLa cells cotreated with particles for 24 h: Au@TNA, Au@TNA@MB, Au@TNA plus 650 nm light, and Au@TNA@MB plus 650 nm light. ( b ) 2’,7’-dichlorofluorescin diacetate (DCFHDA)-stained HeLa cells, ( c ) bright field image of HeLa cells, and ( d ) trypan blue-stained HeLa cells. The cells were stained after 4 h of treatment with Au@TNA@MB and then exposed to 650 nm light. ( e ) Bright field images of HeLa cell apoptosis after pretreatment with 10 ppm [Au] Au@TNA@MB (4 h) plus 650 nm light. The 650 nm laser power density was 125 mW/cm 2 .
    Figure Legend Snippet: ( a ) Thiazolyl blue tetrazolium bromide (MTT) assay of HeLa cells cotreated with particles for 24 h: Au@TNA, Au@TNA@MB, Au@TNA plus 650 nm light, and Au@TNA@MB plus 650 nm light. ( b ) 2’,7’-dichlorofluorescin diacetate (DCFHDA)-stained HeLa cells, ( c ) bright field image of HeLa cells, and ( d ) trypan blue-stained HeLa cells. The cells were stained after 4 h of treatment with Au@TNA@MB and then exposed to 650 nm light. ( e ) Bright field images of HeLa cell apoptosis after pretreatment with 10 ppm [Au] Au@TNA@MB (4 h) plus 650 nm light. The 650 nm laser power density was 125 mW/cm 2 .

    Techniques Used: MTT Assay, Staining

    3) Product Images from "Cytotoxicity of Triterpene Seco-Acids from Betula pubescens Buds"

    Article Title: Cytotoxicity of Triterpene Seco-Acids from Betula pubescens Buds

    Journal: Molecules

    doi: 10.3390/molecules24224060

    Triterpene seco -acids promote apoptosis in AGS and DLD-1 cells. AGS and DLD-1 cells treated with 3,4- seco -urs-4(23),20(30)-dien-3-oic acid ( 1 ), 3,4- seco -olean-4(24)-en-19-oxo-3-oic acid ( 2 ), and 3,4- seco -urs-4(23),20(30)-dien-19-ol-3-oic acid ( 3 ) for 24 h were triple stained with annexin V-FITC conjugate (green fluorescence), propidium iodide (red fluorescence), and Hoechst 33342 (blue fluorescence), and were visualized by fluorescence microscopy. Representative merged photographs are shown. Scale bar = 100 μm. Data are presented as mean ± standard deviation (SD) from three assays. * p
    Figure Legend Snippet: Triterpene seco -acids promote apoptosis in AGS and DLD-1 cells. AGS and DLD-1 cells treated with 3,4- seco -urs-4(23),20(30)-dien-3-oic acid ( 1 ), 3,4- seco -olean-4(24)-en-19-oxo-3-oic acid ( 2 ), and 3,4- seco -urs-4(23),20(30)-dien-19-ol-3-oic acid ( 3 ) for 24 h were triple stained with annexin V-FITC conjugate (green fluorescence), propidium iodide (red fluorescence), and Hoechst 33342 (blue fluorescence), and were visualized by fluorescence microscopy. Representative merged photographs are shown. Scale bar = 100 μm. Data are presented as mean ± standard deviation (SD) from three assays. * p

    Techniques Used: Staining, Fluorescence, Microscopy, Standard Deviation

    4) Product Images from "Metformin Enhances Functional Recovery of Peripheral Nerve in Rats with Sciatic Nerve Crush Injury"

    Article Title: Metformin Enhances Functional Recovery of Peripheral Nerve in Rats with Sciatic Nerve Crush Injury

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.918277

    Metformin enhances the expression of axonal growth and regeneration: The expression of axonal growth and regeneration proteins GAP43 and SCG10 was increased in metformin treated groups, while as the expression of GAP43 and SCG10 was decreased in 3-methyl adenine groups. * p
    Figure Legend Snippet: Metformin enhances the expression of axonal growth and regeneration: The expression of axonal growth and regeneration proteins GAP43 and SCG10 was increased in metformin treated groups, while as the expression of GAP43 and SCG10 was decreased in 3-methyl adenine groups. * p

    Techniques Used: Expressing

    5) Product Images from "Metformin Enhances Functional Recovery of Peripheral Nerve in Rats with Sciatic Nerve Crush Injury"

    Article Title: Metformin Enhances Functional Recovery of Peripheral Nerve in Rats with Sciatic Nerve Crush Injury

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.918277

    Metformin enhances the expression of axonal growth and regeneration: The expression of axonal growth and regeneration proteins GAP43 and SCG10 was increased in metformin treated groups, while as the expression of GAP43 and SCG10 was decreased in 3-methyl adenine groups. * p
    Figure Legend Snippet: Metformin enhances the expression of axonal growth and regeneration: The expression of axonal growth and regeneration proteins GAP43 and SCG10 was increased in metformin treated groups, while as the expression of GAP43 and SCG10 was decreased in 3-methyl adenine groups. * p

    Techniques Used: Expressing

    6) Product Images from "Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor"

    Article Title: Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor

    Journal: Molecules

    doi: 10.3390/molecules25051019

    Effects of gintonin-enriched fraction (GEF) on cell viability and expression of MAPK signaling factors in C2C12 cells under heat-exposed conditions ( a ) Cells were seeded and treated with GEF (20 or 100 μg/mL). After 4 h of pre-incubation, the cells were incubated at 43 °C to induce heat stress; ( b ) Cell viability was measured by thiazolyl blue tetrazolium bromide (MTT) assay in a dose-dependent manner; ( c ) Analysis of p-p38 and p-ERK expression by western blot; ( d ) The density of p-p38 and p-ERK was quantified and expressed as a bar graph. Values labeled with different letters are significantly different ( p
    Figure Legend Snippet: Effects of gintonin-enriched fraction (GEF) on cell viability and expression of MAPK signaling factors in C2C12 cells under heat-exposed conditions ( a ) Cells were seeded and treated with GEF (20 or 100 μg/mL). After 4 h of pre-incubation, the cells were incubated at 43 °C to induce heat stress; ( b ) Cell viability was measured by thiazolyl blue tetrazolium bromide (MTT) assay in a dose-dependent manner; ( c ) Analysis of p-p38 and p-ERK expression by western blot; ( d ) The density of p-p38 and p-ERK was quantified and expressed as a bar graph. Values labeled with different letters are significantly different ( p

    Techniques Used: Expressing, Incubation, MTT Assay, Western Blot, Labeling

    Related Articles

    Staining:

    Article Title: Bioorthogonal Small Molecule Imaging Agents Allow Single-Cell Imaging of MET
    Article Snippet: .. Cell nuclei were stained with Hoechst 33342 (Invitrogen). .. Imaging was done on a DeltaVision microscope (Applied Precision) using a 40X objective.

    Article Title: Apoptosis regulation by subcellular relocation of caspases
    Article Snippet: .. The quality of the obtained nuclei was assessed by DIC (differential interference contrast)/fluorescence microscopy using DMI6000B fluorescent microscope (Leica) following DNA staining with Hoechst33342 (1 mg/ml in PBS) (Molecular Probes) and ER (endoplasmic reticulum) staining with 1 μM ER-tracker Green (BODIPY FL Glibenclamide, Molecular Probes). .. For DNA digestion, the isolated nuclei were resuspended in DNAse buffer (20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 4 mM MgCl2 , 1 mM CaCl2 , 1% NP-40, 0.5 mM DTT, 0.5 mM PMSF and Roche complete protease inhibitors) and incubated with DNAse I (Thermo Scientific) or benzonase (Sigma) for 40 min on ice.).

    Article Title: A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax
    Article Snippet: .. Samples were then incubated with a goat anti-mouse Alexa Fluor® 488-conjugated (1:500 dilution, Invitrogen), goat anti-rabbit Alexa Fluor® 488-conjugated (1:500 dilution, Invitrogen), or donkey anti-goat Cy3-conjugated secondary antibody (1:500 dilution, BioLegend, San Diego, CA, USA), as appropriate; hepatocyte nuclei were stained with 2 µM Hoechst 33342 (Thermo Fisher Scientific). .. Mouse IgG2a, mouse IgG1, rabbit IgG and goat IgG were used as negative control for staining.

    Incubation:

    Article Title: miR-196a Is Able to Restore the Aggressive Phenotype of Annexin A1 Knock-Out in Pancreatic Cancer Cells by CRISPR/Cas9 Genome Editing
    Article Snippet: .. To detect nucleus, samples were incubated with Hoechst 33342 (1:1000; Molecular Probes, Eugene, OR, USA) and excited with a 458 nm Ar laser. ..

    Article Title: The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington’s disease patients
    Article Snippet: .. Then, cells were washed with 0.2% Triton-X/PBS and incubated with secondary antibody (Alexa Fluor 488 Goat anti-Mouse (ThermoFisher Scientific, #A-11029, 1:500), Alexa Fluor 568F(ab’)2 Fragment of Goat Anti-Rabbit IgG (H+L) (ThermoFisher Scientific, #A-21069, 1:500)), Alexa Fluor 647 Donkey anti-Chicken (Jackson ImmunoResearch, #A-703-605-155, 1:500) and Hoechst 33342 (Life Technologies, #1656104) for 1 h at room temperature. .. PBS and distilled water wash were followed before the cover slips were mounted on Mowiol (Sigma, #324590).

    Article Title: A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax
    Article Snippet: .. Samples were then incubated with a goat anti-mouse Alexa Fluor® 488-conjugated (1:500 dilution, Invitrogen), goat anti-rabbit Alexa Fluor® 488-conjugated (1:500 dilution, Invitrogen), or donkey anti-goat Cy3-conjugated secondary antibody (1:500 dilution, BioLegend, San Diego, CA, USA), as appropriate; hepatocyte nuclei were stained with 2 µM Hoechst 33342 (Thermo Fisher Scientific). .. Mouse IgG2a, mouse IgG1, rabbit IgG and goat IgG were used as negative control for staining.

    Article Title: Tranilast inhibits the expression of genes related to epithelial-mesenchymal transition and angiogenesis in neurofibromin-deficient cells
    Article Snippet: .. They were then fixed for 30 min at room temperature with 4% paraformaldehyde in PBS, washed with PBS, incubated for 60 min at room temperature with Hoechst 33342 (Invitrogen) and phalloidin–Alexa Fluor 568 (Invitrogen) in PBS, and washed again with PBS. .. The cells were examined with a high-throughput image screening system (ImageXpress ULTRA, Molecular Devices) for quantitation of focus formation, with a focus being defined as a cell aggregate with an area of Hoechst 33342 fluorescence greater than a certain threshold.

    other:

    Article Title: Apoptotic and Anti-Inflammatory Effects of Eupatorium japonicum Thunb. in Rheumatoid Arthritis Fibroblast-Like Synoviocytes
    Article Snippet: Hoechst 33342 was purchased from Invitrogen (CA).

    Microscopy:

    Article Title: Apoptosis regulation by subcellular relocation of caspases
    Article Snippet: .. The quality of the obtained nuclei was assessed by DIC (differential interference contrast)/fluorescence microscopy using DMI6000B fluorescent microscope (Leica) following DNA staining with Hoechst33342 (1 mg/ml in PBS) (Molecular Probes) and ER (endoplasmic reticulum) staining with 1 μM ER-tracker Green (BODIPY FL Glibenclamide, Molecular Probes). .. For DNA digestion, the isolated nuclei were resuspended in DNAse buffer (20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 4 mM MgCl2 , 1 mM CaCl2 , 1% NP-40, 0.5 mM DTT, 0.5 mM PMSF and Roche complete protease inhibitors) and incubated with DNAse I (Thermo Scientific) or benzonase (Sigma) for 40 min on ice.).

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    Thermo Fisher mtt reagent
    Effect of PLAB on U87 glioblastoma cell viability. (a) U87 cells were treated with indicated concentrations of PLAB and <t>doxorubicin</t> (positive control) for 24 h and cell viability was determined by <t>MTT</t> assay. Data are expressed as Mean ± SEM ( n = 3). Columns not sharing the same superscript letter differ significantly ( P
    Mtt Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtt reagent/product/Thermo Fisher
    Average 99 stars, based on 167 article reviews
    Price from $9.99 to $1999.99
    mtt reagent - by Bioz Stars, 2021-01
    99/100 stars
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    Effect of PLAB on U87 glioblastoma cell viability. (a) U87 cells were treated with indicated concentrations of PLAB and doxorubicin (positive control) for 24 h and cell viability was determined by MTT assay. Data are expressed as Mean ± SEM ( n = 3). Columns not sharing the same superscript letter differ significantly ( P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells

    doi: 10.1155/2012/957568

    Figure Lengend Snippet: Effect of PLAB on U87 glioblastoma cell viability. (a) U87 cells were treated with indicated concentrations of PLAB and doxorubicin (positive control) for 24 h and cell viability was determined by MTT assay. Data are expressed as Mean ± SEM ( n = 3). Columns not sharing the same superscript letter differ significantly ( P

    Article Snippet: Briefly U87 cells were treated with various concentrations of PLAB (1 to 100 μ M) or Doxorubicin (0.25 to 20 μ M) for 24 h. Following treatment, the MTT reagent was added (500 μ g/mL) and cells were further incubated at 37°C for 4 h. Subsequently 150 μ L DMSO was added to dissolve farmazan crystals and absorbance was measured at 570 nm in a microplate reader (Thermo Scientific).

    Techniques: Positive Control, MTT Assay

    Downregulation of miR-1291 promotes proliferation and cell cycle of LNCaP cells. (A) LNCaP cells were transfected with miR-1291 inhibitors or INC. (B) Cell viability was detected by MTT assay. (C) After transfection for 48 h, LNCaP cells were stained with PI, and the distribution of cell cycle was determined using flow cytometric analysis. *P

    Journal: Oncology Letters

    Article Title: MicroRNA-1291 mediates cell proliferation and tumorigenesis by downregulating MED1 in prostate cancer

    doi: 10.3892/ol.2019.9980

    Figure Lengend Snippet: Downregulation of miR-1291 promotes proliferation and cell cycle of LNCaP cells. (A) LNCaP cells were transfected with miR-1291 inhibitors or INC. (B) Cell viability was detected by MTT assay. (C) After transfection for 48 h, LNCaP cells were stained with PI, and the distribution of cell cycle was determined using flow cytometric analysis. *P

    Article Snippet: A total of 3.5×103 cells were seeded into 96-well plates per well after treatment with miR-1291 mimics, NC, inhibitors or INC. At 0, 24, 48 and 72 h, 0.5 mg/ml MTT buffer (Thermo Fisher Scientific, Inc.) was added per well and cells were cultured for 2 h in darkness.

    Techniques: Transfection, MTT Assay, Staining, Flow Cytometry

    Generation and in vitro evaluation of anti-HER2 ARC-ADC. ( A ) Scheme for generating anti-HER2 ARC-ADC. r.t., room temperature. ( B ) Conjugation kinetics of drug linker to CD38 C-fusion IgG. 2′-Cl-araNAD + –MMAF (1 mM) was incubated with CD38 C-fusion IgG (10 μM) in 50 mM tris buffer (pH 8.5) for various amounts of time on ice. The residual enzymatic activity determined by fluorescence-based activity assays was plotted as a function of incubation time. ( C and D ) Mass spectra of light chains (C) and heavy chains (D) for CD38 C-fusion IgG and anti-HER2 ARC-ADC. MW, molecular weight. ( E ) X-ray structure of human CD38 catalytic domain with 2′-Cl-araNAD + covalently attached to Glu 226 residue. ( F ) Flow cytometric analysis of HER2 expression for four breast cancer cell lines. ( G to J ) In vitro cytotoxicity of anti-HER2 ARC-ADC. HCC1954 (G), MCF7 (H), MDA-MB-231 (I), and MDA-MB-468 (J) cells with varied levels of HER2 expression were incubated for 72 hours at 37°C with 5% CO 2 in the presence of various concentrations of ARC-ADC, 2′-Cl-araNAD + –MMAF, Herceptin, and CD38 C-fusion IgG. Cell viability was measured by MTT assays. Cells treated with culture media and 5 μM paclitaxel were included as 100% viability and 0% viability controls, respectively.

    Journal: Science Advances

    Article Title: Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases

    doi: 10.1126/sciadv.aba6752

    Figure Lengend Snippet: Generation and in vitro evaluation of anti-HER2 ARC-ADC. ( A ) Scheme for generating anti-HER2 ARC-ADC. r.t., room temperature. ( B ) Conjugation kinetics of drug linker to CD38 C-fusion IgG. 2′-Cl-araNAD + –MMAF (1 mM) was incubated with CD38 C-fusion IgG (10 μM) in 50 mM tris buffer (pH 8.5) for various amounts of time on ice. The residual enzymatic activity determined by fluorescence-based activity assays was plotted as a function of incubation time. ( C and D ) Mass spectra of light chains (C) and heavy chains (D) for CD38 C-fusion IgG and anti-HER2 ARC-ADC. MW, molecular weight. ( E ) X-ray structure of human CD38 catalytic domain with 2′-Cl-araNAD + covalently attached to Glu 226 residue. ( F ) Flow cytometric analysis of HER2 expression for four breast cancer cell lines. ( G to J ) In vitro cytotoxicity of anti-HER2 ARC-ADC. HCC1954 (G), MCF7 (H), MDA-MB-231 (I), and MDA-MB-468 (J) cells with varied levels of HER2 expression were incubated for 72 hours at 37°C with 5% CO 2 in the presence of various concentrations of ARC-ADC, 2′-Cl-araNAD + –MMAF, Herceptin, and CD38 C-fusion IgG. Cell viability was measured by MTT assays. Cells treated with culture media and 5 μM paclitaxel were included as 100% viability and 0% viability controls, respectively.

    Article Snippet: After 3-day incubation at 37°C with 5% CO2 , 10 μl of MTT reagent (Thermo Fisher Scientific, MA) was added, followed by 2-hour incubation at 37°C in an incubator with 5% CO2 .

    Techniques: In Vitro, Conjugation Assay, Incubation, Activity Assay, Fluorescence, Molecular Weight, Expressing, Multiple Displacement Amplification, MTT Assay

    Antiproliferative effects of TBMS1 on growth and morphological characteristics of PC3 prostate cancer cells. (A) PC3 cells were treated with various concentrations of TBMS1, and cell viability was evaluated with a MTT assay. (B) PC3 cells were treated with 15 μmol/L TBMS1 in the presence or absence of 3 mmol/L NAC, cell viability was determined with a MTT assay. Data are expressed as the mean±SEM of three independent experiments. * P

    Journal: Acta Pharmacologica Sinica

    Article Title: Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro

    doi: 10.1038/aps.2016.34

    Figure Lengend Snippet: Antiproliferative effects of TBMS1 on growth and morphological characteristics of PC3 prostate cancer cells. (A) PC3 cells were treated with various concentrations of TBMS1, and cell viability was evaluated with a MTT assay. (B) PC3 cells were treated with 15 μmol/L TBMS1 in the presence or absence of 3 mmol/L NAC, cell viability was determined with a MTT assay. Data are expressed as the mean±SEM of three independent experiments. * P

    Article Snippet: Briefly, DU145 and PC3 cells were seeded in 96-well tissue culture plates and incubated in a CO2 incubator for 24 h, and the cells were then exposed to different concentrations of TBMS1 (1–100 μmol/L) for 24 h. Following treatment, 10 μL MTT reagent (5 mg/mL) was added to each well, and cells were further incubated at 37 °C for 4 h. Subsequently, 150 μL DMSO was added to dissolve the formazan crystals, and absorbance was measured at 570 nm in a microplate reader (Thermo Scientific, Varioskan Flash, USA).

    Techniques: MTT Assay

    Antiproliferative effects of TBMS1 on growth and morphological characteristics of DU145 prostate cancer cells. (A) Chemical structure of TBMS1. (B) Dose-dependent effect of TBMS1 on the viability of DU145 cells. The cells were treated with the indicated concentrations of TBMS1 for 24 h. Cell viability was evaluated using the MTT assay. Data are expressed as the mean±SEM of three independent experiments. * P

    Journal: Acta Pharmacologica Sinica

    Article Title: Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro

    doi: 10.1038/aps.2016.34

    Figure Lengend Snippet: Antiproliferative effects of TBMS1 on growth and morphological characteristics of DU145 prostate cancer cells. (A) Chemical structure of TBMS1. (B) Dose-dependent effect of TBMS1 on the viability of DU145 cells. The cells were treated with the indicated concentrations of TBMS1 for 24 h. Cell viability was evaluated using the MTT assay. Data are expressed as the mean±SEM of three independent experiments. * P

    Article Snippet: Briefly, DU145 and PC3 cells were seeded in 96-well tissue culture plates and incubated in a CO2 incubator for 24 h, and the cells were then exposed to different concentrations of TBMS1 (1–100 μmol/L) for 24 h. Following treatment, 10 μL MTT reagent (5 mg/mL) was added to each well, and cells were further incubated at 37 °C for 4 h. Subsequently, 150 μL DMSO was added to dissolve the formazan crystals, and absorbance was measured at 570 nm in a microplate reader (Thermo Scientific, Varioskan Flash, USA).

    Techniques: MTT Assay