3 3 diaminobenzidine  (Vector Laboratories)


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    Name:
    DAB Peroxidase HRP Substrate Kit with Nickel 3 3 diaminobenzidine
    Description:
    DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
    Catalog Number:
    sk-4100
    Price:
    None
    Category:
    Proteins
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories 3 3 diaminobenzidine
    DAB Peroxidase HRP Substrate Kit with Nickel 3 3 diaminobenzidine
    DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
    https://www.bioz.com/result/3 3 diaminobenzidine/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Changes of the Structural and Biomechanical Properties of the Bovine Pericardium after the Removal of ?-Gal Epitopes by Decellularization and ?-Galactosidase Treatment"

    Article Title: Changes of the Structural and Biomechanical Properties of the Bovine Pericardium after the Removal of ?-Gal Epitopes by Decellularization and ?-Galactosidase Treatment

    Journal: The Korean Journal of Thoracic and Cardiovascular Surgery

    doi: 10.5090/kjtcs.2012.45.6.380

    Removal of α-gal epitopes on the bovine pericardium by recombinant Bacterides thetaiotaomicron α-galactosidase using lectin histochemistry. Fresh bovine pericardium (D). Bovine pericardium treated with decellularization (E). Bovine pericardium treated with decellularization and α-galactosidase (F). (A), (B), and (C) were negative control of (D), (E), and (F), respectively. The frozen tissue samples were stained with biotinylated lectin and avidin-peroxidase to detect α-gal epitope. 3,3'-Diaminobenzidine staining intensity of tissues was captured by light microscopy (×400).
    Figure Legend Snippet: Removal of α-gal epitopes on the bovine pericardium by recombinant Bacterides thetaiotaomicron α-galactosidase using lectin histochemistry. Fresh bovine pericardium (D). Bovine pericardium treated with decellularization (E). Bovine pericardium treated with decellularization and α-galactosidase (F). (A), (B), and (C) were negative control of (D), (E), and (F), respectively. The frozen tissue samples were stained with biotinylated lectin and avidin-peroxidase to detect α-gal epitope. 3,3'-Diaminobenzidine staining intensity of tissues was captured by light microscopy (×400).

    Techniques Used: Recombinant, Negative Control, Staining, Avidin-Biotin Assay, Light Microscopy

    Related Articles

    Immunohistochemistry:

    Article Title: Cell-based quantification of molecular biomarkers in histopathology specimens
    Article Snippet: Antibodies used for immunostaining included monoclonal mouse anti-human oestrogen receptor (ER), anti-human progesterone receptor (PR), anti-human Ki67, anti-epithelial membrane antigen (EMA), rabbit polyclonal anti-human epidermal growth factor receptor 2 (HER2) (Dako, Carpenteria, CA, USA), rabbit anti-phospho(p)-extracellular signal-related kinase (ERK), anti-p-S6 (Cell Signaling, Danvers, MA, USA), and mouse anti-multi-cytokeratin (CK) monoclonal antibodies (Vector Laboratories, Burlingame, CA, USA). .. ER, PR, Ki67, p-ERK and HER2 were detected by immunohistochemistry with biotinylated species-specific secondary antibodies, avidin-linked horseradish peroxidase (HRP) (ABC Kit) and 3,3-diaminobenzidine or SG Blue (Vector Laboratories) HRP chromogen substrate. .. CK, EMA and p-S6 immunostaining were detected by fluorescence, with the use of Zenon Alexa Fluor-488 mouse IgG1 labelling (Invitrogen, Carlsbad, CA, USA), fluorescently labelled secondary antibodies (Invitrogen) or the ABC fluorescence detection kit.

    Avidin-Biotin Assay:

    Article Title: Cell-based quantification of molecular biomarkers in histopathology specimens
    Article Snippet: Antibodies used for immunostaining included monoclonal mouse anti-human oestrogen receptor (ER), anti-human progesterone receptor (PR), anti-human Ki67, anti-epithelial membrane antigen (EMA), rabbit polyclonal anti-human epidermal growth factor receptor 2 (HER2) (Dako, Carpenteria, CA, USA), rabbit anti-phospho(p)-extracellular signal-related kinase (ERK), anti-p-S6 (Cell Signaling, Danvers, MA, USA), and mouse anti-multi-cytokeratin (CK) monoclonal antibodies (Vector Laboratories, Burlingame, CA, USA). .. ER, PR, Ki67, p-ERK and HER2 were detected by immunohistochemistry with biotinylated species-specific secondary antibodies, avidin-linked horseradish peroxidase (HRP) (ABC Kit) and 3,3-diaminobenzidine or SG Blue (Vector Laboratories) HRP chromogen substrate. .. CK, EMA and p-S6 immunostaining were detected by fluorescence, with the use of Zenon Alexa Fluor-488 mouse IgG1 labelling (Invitrogen, Carlsbad, CA, USA), fluorescently labelled secondary antibodies (Invitrogen) or the ABC fluorescence detection kit.

    Immunolabeling:

    Article Title: Connective Tissue Growth Factor Modulates Adult β-Cell Maturity and Proliferation to Promote β-Cell Regeneration in Mice
    Article Snippet: Imaging was with a ScanScope FL scanner (Aperio Technologies, Inc.) and quantified using Metamorph 6.1 (Molecular Devices). .. β-Cell Mass Ten to 12 slides per animal (1 to 2% of entire pancreas) were immunolabeled for insulin, visualized via the DAB Peroxidase Substrate Kit (Vector Laboratories), and counterstained with eosin. ..

    Incubation:

    Article Title: CRE/CREB-Driven Up-Regulation of Gene Expression by Chronic Social Stress in CRE-Luciferase Transgenic Mice: Reversal by Antidepressant Treatment
    Article Snippet: .. Sections were washed intensively and than incubated with rabbit anti goat antibody (DAKO, Hamburg, Germany, diluted 1∶100) for 4 h. After washing, sections were incubated with anti-rabbit-PAP (DAKO, diluted 1∶100 in blocking buffer) for 2 h. Luciferase immunoreactivity was developed by incubating the sections for 5 min in 50 mM Tris pH 7.2 with 0.6 mg/ml of 3,3′-diaminobenzidine (DAB) and 0.3 mg/ml H2 O2 (Vector Labs/Alexis Deutschland, Grünberg, Germany). ..

    Blocking Assay:

    Article Title: CRE/CREB-Driven Up-Regulation of Gene Expression by Chronic Social Stress in CRE-Luciferase Transgenic Mice: Reversal by Antidepressant Treatment
    Article Snippet: .. Sections were washed intensively and than incubated with rabbit anti goat antibody (DAKO, Hamburg, Germany, diluted 1∶100) for 4 h. After washing, sections were incubated with anti-rabbit-PAP (DAKO, diluted 1∶100 in blocking buffer) for 2 h. Luciferase immunoreactivity was developed by incubating the sections for 5 min in 50 mM Tris pH 7.2 with 0.6 mg/ml of 3,3′-diaminobenzidine (DAB) and 0.3 mg/ml H2 O2 (Vector Labs/Alexis Deutschland, Grünberg, Germany). ..

    Luciferase:

    Article Title: CRE/CREB-Driven Up-Regulation of Gene Expression by Chronic Social Stress in CRE-Luciferase Transgenic Mice: Reversal by Antidepressant Treatment
    Article Snippet: .. Sections were washed intensively and than incubated with rabbit anti goat antibody (DAKO, Hamburg, Germany, diluted 1∶100) for 4 h. After washing, sections were incubated with anti-rabbit-PAP (DAKO, diluted 1∶100 in blocking buffer) for 2 h. Luciferase immunoreactivity was developed by incubating the sections for 5 min in 50 mM Tris pH 7.2 with 0.6 mg/ml of 3,3′-diaminobenzidine (DAB) and 0.3 mg/ml H2 O2 (Vector Labs/Alexis Deutschland, Grünberg, Germany). ..

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  • 86
    Vector Laboratories chromogen 3 3 diaminobenzidine
    The presence of NETs (MPO and histones: H2A, H2B, and H3) in the chorion and the placental decidua. Chromogen 3.3′-diaminobenzidine: (1) anti-MPO, (2) anti-H2A, (3) anti-H2B, (4) anti-H3 antibodies; ( A ) control group, without granulocytes (magnification 200x); ( B ) women with miscarriage, with granulocytes (magnification 400x); ( C ) women with miscarriage, with NETs (arrows) (magnification 400x).
    Chromogen 3 3 Diaminobenzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromogen 3 3 diaminobenzidine/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chromogen 3 3 diaminobenzidine - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    97
    Vector Laboratories 3 diaminobenzidine
    CK1 and AH1 replication profile in mouse lung. A. Representative images of immunohistochemically stained influenza nucleoprotein (NP) in formalin fixed mouse lung tissue infected with CK1 or AH1 at day 2 p.i. Viral NP protein was labeled brown by <t>3,3′-diaminobenzidine</t> (DAB). Uninfected mouse lung as negative control ( a) , amplified image (b); Representative images of CK1(c) and AH1 (d) infected mouse lung stained NP positive at 40x magnification. Trachea epithelial cells (e and f), bronchiole epithelial and alveolar epithelial cells (g and h) were stained positive for viral NP protein. (Original magnification ×200). B. Viral load in infected mouse lung homogenates. Mice were infected with 10 5 of AH1 or 10 6 PFU of CK1, at day 1, 2, 4 and 6 p.i., 3–5 mice from each group were sacrificed. The left side of the lung was homogenized in 1 ml of MEM culture medium. Viral loads were determined by amplification of viral M gene copy numbers by real time RT-PCR (top panel), and infectious viral titre were determined by TCID 50 assay on MDCK cells (bottom panel). ** P
    3 Diaminobenzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 diaminobenzidine/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    The presence of NETs (MPO and histones: H2A, H2B, and H3) in the chorion and the placental decidua. Chromogen 3.3′-diaminobenzidine: (1) anti-MPO, (2) anti-H2A, (3) anti-H2B, (4) anti-H3 antibodies; ( A ) control group, without granulocytes (magnification 200x); ( B ) women with miscarriage, with granulocytes (magnification 400x); ( C ) women with miscarriage, with NETs (arrows) (magnification 400x).

    Journal: Scientific Reports

    Article Title: Biomarkers of neutrophil extracellular traps (NETs) and nitric oxide-(NO)-dependent oxidative stress in women who miscarried

    doi: 10.1038/s41598-020-70106-x

    Figure Lengend Snippet: The presence of NETs (MPO and histones: H2A, H2B, and H3) in the chorion and the placental decidua. Chromogen 3.3′-diaminobenzidine: (1) anti-MPO, (2) anti-H2A, (3) anti-H2B, (4) anti-H3 antibodies; ( A ) control group, without granulocytes (magnification 200x); ( B ) women with miscarriage, with granulocytes (magnification 400x); ( C ) women with miscarriage, with NETs (arrows) (magnification 400x).

    Article Snippet: After a reaction induced through the use of a polymeric technique (ImmPRESS, Vector Laboratories) the antigen–antibody complex was exposed by incubation with chromogen 3.3′-diaminobenzidine (DAB, Vector Laboratories).

    Techniques:

    CK1 and AH1 replication profile in mouse lung. A. Representative images of immunohistochemically stained influenza nucleoprotein (NP) in formalin fixed mouse lung tissue infected with CK1 or AH1 at day 2 p.i. Viral NP protein was labeled brown by 3,3′-diaminobenzidine (DAB). Uninfected mouse lung as negative control ( a) , amplified image (b); Representative images of CK1(c) and AH1 (d) infected mouse lung stained NP positive at 40x magnification. Trachea epithelial cells (e and f), bronchiole epithelial and alveolar epithelial cells (g and h) were stained positive for viral NP protein. (Original magnification ×200). B. Viral load in infected mouse lung homogenates. Mice were infected with 10 5 of AH1 or 10 6 PFU of CK1, at day 1, 2, 4 and 6 p.i., 3–5 mice from each group were sacrificed. The left side of the lung was homogenized in 1 ml of MEM culture medium. Viral loads were determined by amplification of viral M gene copy numbers by real time RT-PCR (top panel), and infectious viral titre were determined by TCID 50 assay on MDCK cells (bottom panel). ** P

    Journal: PLoS ONE

    Article Title: Avian Influenza A H7N9 Virus Induces Severe Pneumonia in Mice without Prior Adaptation and Responds to a Combination of Zanamivir and COX-2 Inhibitor

    doi: 10.1371/journal.pone.0107966

    Figure Lengend Snippet: CK1 and AH1 replication profile in mouse lung. A. Representative images of immunohistochemically stained influenza nucleoprotein (NP) in formalin fixed mouse lung tissue infected with CK1 or AH1 at day 2 p.i. Viral NP protein was labeled brown by 3,3′-diaminobenzidine (DAB). Uninfected mouse lung as negative control ( a) , amplified image (b); Representative images of CK1(c) and AH1 (d) infected mouse lung stained NP positive at 40x magnification. Trachea epithelial cells (e and f), bronchiole epithelial and alveolar epithelial cells (g and h) were stained positive for viral NP protein. (Original magnification ×200). B. Viral load in infected mouse lung homogenates. Mice were infected with 10 5 of AH1 or 10 6 PFU of CK1, at day 1, 2, 4 and 6 p.i., 3–5 mice from each group were sacrificed. The left side of the lung was homogenized in 1 ml of MEM culture medium. Viral loads were determined by amplification of viral M gene copy numbers by real time RT-PCR (top panel), and infectious viral titre were determined by TCID 50 assay on MDCK cells (bottom panel). ** P

    Article Snippet: Streptavidin/peroxidase complex reagent (Vector Laboratories, Burlingame, CA) was then added and incubated at room temperature for 30 min. Colour development was performed with 3, 3′-diaminobenzidine (DAB, Vector Laboratories, Burlingame, CA, USA) and images were captured with Nikon80i imaging system with the help of Spot-advance computer software.

    Techniques: Staining, Infection, Labeling, Negative Control, Amplification, Mouse Assay, Quantitative RT-PCR

    CXCR4 and ACKR3 staining using IHC in breast cancer tissue. 4 μm sections from human breast cancer were stained for CXCR4 (1:40) and ACKR3 (1:100) using immunohistochemistry following no pre-treatment or EDTA antigen retrieval pre-treatment, respectively. Briefly, protocol from the VECTASTAIN ABC HRP kit was followed, signal was developed using DAB and counterstained with haematoxylin. No primary antibody was used as a control. n = 2.

    Journal: International Journal of Molecular Sciences

    Article Title: Breast Cancer: An Examination of the Potential of ACKR3 to Modify the Response of CXCR4 to CXCL12

    doi: 10.3390/ijms19113592

    Figure Lengend Snippet: CXCR4 and ACKR3 staining using IHC in breast cancer tissue. 4 μm sections from human breast cancer were stained for CXCR4 (1:40) and ACKR3 (1:100) using immunohistochemistry following no pre-treatment or EDTA antigen retrieval pre-treatment, respectively. Briefly, protocol from the VECTASTAIN ABC HRP kit was followed, signal was developed using DAB and counterstained with haematoxylin. No primary antibody was used as a control. n = 2.

    Article Snippet: Secondary and tertiary antibodies were added for 30 min before dispensing DAB on top of the samples (DAB Peroxidase (HRP) Substrate Kit, Vector labs, Burlingame, CA, USA) and counterstaining with haematoxylin.

    Techniques: Staining, Immunohistochemistry

    Immunostaining of monokine induced by interferon γ (Mig)/CXCL9, interferon-inducible protein-10 (IP-10)/CXCL10 and interferon-inducible T cell α-chemoattractant (I-TAC)/CXCL11 in bronchoalveolar lavage (BA) cells and sarcoid lungs. BAL cells were cytocentrifuged on glass slides and fixed. The slides were incubated with control rabbit serum (a), anti-Mig/CXCL9 (b), IP-10/CXCL10 (c) or I-TAC/CXCL11 antibody (d), and stained with Alexa fluor 488-conjugated goat anti-rabbit IgG (H + l) antibody (green fluorescence) and 4′,6 diamidino-2-phenylindole (DAPI; by which nuclei were counterstained) (blue). Immunostaining was visualized using a fluorescence microscope (original magnifications: × 400). Arrows point to the lymphocytes which are negative for CXCR3 ligands. Freshly frozen lung sections derived from three patients with stage II of sarcoidosis were also stained with control rabbit serum (e), anti-Mig/CXCL9 (f), IP-10/CXCL10 (g) or I-TAC/CXCL11 antibody (h) and RTU Vectastain Universal Quick Kit, and developed with diaminobenzidine (DAB) substrate (original magnifications: × 200). The insets in e, f, g and h show the staining of macrophages in the alveolar lumen (original magnifications: × 400). Scale bars = 50 µm.

    Journal: Clinical and Experimental Immunology

    Article Title: CXCL9 and 11 in patients with pulmonary sarcoidosis: a role of alveolar macrophages

    doi: 10.1111/j.1365-2249.2007.03423.x

    Figure Lengend Snippet: Immunostaining of monokine induced by interferon γ (Mig)/CXCL9, interferon-inducible protein-10 (IP-10)/CXCL10 and interferon-inducible T cell α-chemoattractant (I-TAC)/CXCL11 in bronchoalveolar lavage (BA) cells and sarcoid lungs. BAL cells were cytocentrifuged on glass slides and fixed. The slides were incubated with control rabbit serum (a), anti-Mig/CXCL9 (b), IP-10/CXCL10 (c) or I-TAC/CXCL11 antibody (d), and stained with Alexa fluor 488-conjugated goat anti-rabbit IgG (H + l) antibody (green fluorescence) and 4′,6 diamidino-2-phenylindole (DAPI; by which nuclei were counterstained) (blue). Immunostaining was visualized using a fluorescence microscope (original magnifications: × 400). Arrows point to the lymphocytes which are negative for CXCR3 ligands. Freshly frozen lung sections derived from three patients with stage II of sarcoidosis were also stained with control rabbit serum (e), anti-Mig/CXCL9 (f), IP-10/CXCL10 (g) or I-TAC/CXCL11 antibody (h) and RTU Vectastain Universal Quick Kit, and developed with diaminobenzidine (DAB) substrate (original magnifications: × 200). The insets in e, f, g and h show the staining of macrophages in the alveolar lumen (original magnifications: × 400). Scale bars = 50 µm.

    Article Snippet: Sections were developed with a diaminobenzidine substrate kit (Vector Laboratories, Inc.) and counterstained with Mayer's haematoxylin (Muto Pure Chemicals Co., Ltd, Tokyo, Japan).

    Techniques: Immunostaining, Incubation, Staining, Fluorescence, Microscopy, Derivative Assay