3 diaminobenzidine tetrahydrochloride  (Thermo Fisher)

 
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    Name:
    DAB Substrate Powder 3 3 diaminobenzidine tetrahydrochloride
    Description:
    Thermo Scientific Pierce DAB enables chromogenic detection of horseradish peroxidase HRP activity based the action of 3 3 diaminobenzidine DAB in Western blot and tissue staining methods DAB 3 3 diaminobenzidine tetrahydrochloride MW 214 1 reacts with HRP in the presence of peroxide to yield an insoluble brown colored product at locations where peroxidase conjugated antibodies are bound to samples The brown precipitate is insoluble in alcohol and other organic solvents making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media Features of DAB Powder • HRP substrate for detection of horseradish peroxidase activity on solid media including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic no special equipment needed for visualization the DAB reaction produces brown bands or spots at sites of reaction with HRP conjugated antibodies or probes • Package options choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use DAB has been used in a variety of applications including Western blotting immunohistochemistry and electron microscopy Many enhancement methods have been reported for DAB involving different buffer conditions the addition of metal ions and post treatment applications Ref 1 7 also see the Pierce Metal Enhanced DAB Substrate Kit Part No 34065 One disadvantage of DAB compared to other substrates is the need to use the working solution immediately because it begins a reaction process upon the addition of hydrogen peroxide This results in increased background color of the substrate However the brown bands resulting from DAB reactions do not fade as quickly as the color products of other precipitating HRP substrates Related Products Pierce DAB Substrate Kit
    Catalog Number:
    34001
    Price:
    None
    Applications:
    Cell Analysis|Cellular Imaging|IHC Staining & Detection|Immunohistochemistry (IHC)|Protein Assays and Analysis|Protein Biology|Western Blotting
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher 3 diaminobenzidine tetrahydrochloride
    Thermo Scientific Pierce DAB enables chromogenic detection of horseradish peroxidase HRP activity based the action of 3 3 diaminobenzidine DAB in Western blot and tissue staining methods DAB 3 3 diaminobenzidine tetrahydrochloride MW 214 1 reacts with HRP in the presence of peroxide to yield an insoluble brown colored product at locations where peroxidase conjugated antibodies are bound to samples The brown precipitate is insoluble in alcohol and other organic solvents making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media Features of DAB Powder • HRP substrate for detection of horseradish peroxidase activity on solid media including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic no special equipment needed for visualization the DAB reaction produces brown bands or spots at sites of reaction with HRP conjugated antibodies or probes • Package options choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use DAB has been used in a variety of applications including Western blotting immunohistochemistry and electron microscopy Many enhancement methods have been reported for DAB involving different buffer conditions the addition of metal ions and post treatment applications Ref 1 7 also see the Pierce Metal Enhanced DAB Substrate Kit Part No 34065 One disadvantage of DAB compared to other substrates is the need to use the working solution immediately because it begins a reaction process upon the addition of hydrogen peroxide This results in increased background color of the substrate However the brown bands resulting from DAB reactions do not fade as quickly as the color products of other precipitating HRP substrates Related Products Pierce DAB Substrate Kit
    https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2021-02
    99/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin–Angiotensin System
    Article Snippet: .. 3,3-Diaminobenzidine (DAB) IHC staining for SOX2 (1:500; cat# PA094, Thermo Fisher, Scientific, Scoresby, VIC, Australia), PRR (1:2000; cat# ab40790, Abcam, Cambridge, UK), ATIIR1 (1:30; cat# ab9391, Abcam), ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, LLC, Littleton, CO, USA), ACE (1:100; cat# MCA2054, AbD Serotec, Kidlington, UK) diluted with Bond™ primary antibody diluent (cat# AR9352, Leica) was done for all tissue samples. .. Immunofluorescent (IF) IHC staining was performed on two representative GBM tissue samples from the original cohort of patients used for DAB IHC staining, using identical primary antibodies and concentrations.

    Article Title: NADPH oxidase 1 mediates caerulein-induced pancreatic fibrosis in chronic pancreatitis
    Article Snippet: .. IHC for α-SMA, NF-ĸB and p-AKT in whole pancreas: Cells positive for these proteins were determined using 3,3-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (color: brown). .. Briefly, slides were incubated first with blocking buffer (3% BSA in PBS with 0.05% Tween-20) for 1 h at room temperature and then with antibody against α-SMA, NF-ĸB or p-AKT overnight at 4 °C.

    Avidin-Biotin Assay:

    Article Title: The Effects of Maekmoondong-Tang on Cockroach Extract-Induced Allergic Asthma
    Article Snippet: .. After the slides were incubated with avidin-biotin peroxidase complex (ABC kit, Vestor Laboratories, CA, USA), the color was developed with 3,3′-diaminobenzidine tetrachloride (DAB; Zymed Laboratories, CA, USA). .. After immunohistochemical staining, the slides were counterstained with Harris's hematoxylin for 1 minute and then mounted with Canada balsam (Show Chemical Co. Ltd., Tokyo, Japan).

    Immunostaining:

    Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins
    Article Snippet: .. For immunostaining, cells at about 100% confluency were fixed with 2% paraformaldehyde-0.2% glutaraldehyde and stained with anti-myc (1:1,000) followed by anti-mouse secondary antibody and diaminobenzidine substrate (Lifetech). .. For FACS of transiently expressed CB5, A7 monolayers were transfected with pB5myc or vector only.

    Incubation:

    Article Title: The Effects of Maekmoondong-Tang on Cockroach Extract-Induced Allergic Asthma
    Article Snippet: .. After the slides were incubated with avidin-biotin peroxidase complex (ABC kit, Vestor Laboratories, CA, USA), the color was developed with 3,3′-diaminobenzidine tetrachloride (DAB; Zymed Laboratories, CA, USA). .. After immunohistochemical staining, the slides were counterstained with Harris's hematoxylin for 1 minute and then mounted with Canada balsam (Show Chemical Co. Ltd., Tokyo, Japan).

    Expressing:

    Article Title: Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila
    Article Snippet: .. HRP expression was revealed overnight using the DAB substrate kit (Thermofisher). ..

    Staining:

    Article Title: Downregulation of claudin-7 potentiates cellular proliferation and invasion in endometrial cancer
    Article Snippet: .. Antibody staining was visualized with 3,3′-diaminobenzidine (DAB; Invitrogen Life Technologies, Carlsbad, CA, USA). .. For evaluation, the following criteria were used: 0, no expression (complete negative staining); 1, weak expression (1–15% positive staining); 2, moderate expression (16–49% positive staining); and 3, strong expression (50–100% positive staining).

    Article Title: Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin–Angiotensin System
    Article Snippet: .. 3,3-Diaminobenzidine (DAB) IHC staining for SOX2 (1:500; cat# PA094, Thermo Fisher, Scientific, Scoresby, VIC, Australia), PRR (1:2000; cat# ab40790, Abcam, Cambridge, UK), ATIIR1 (1:30; cat# ab9391, Abcam), ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, LLC, Littleton, CO, USA), ACE (1:100; cat# MCA2054, AbD Serotec, Kidlington, UK) diluted with Bond™ primary antibody diluent (cat# AR9352, Leica) was done for all tissue samples. .. Immunofluorescent (IF) IHC staining was performed on two representative GBM tissue samples from the original cohort of patients used for DAB IHC staining, using identical primary antibodies and concentrations.

    Article Title: Central nervous system neurons acquire mast cell products via transgranulation
    Article Snippet: .. The first made use of conventional staining with 3,3′ diaminobenzidine (DAB) (Immunopure DAB, Pierce) and the second, a silver intensification followed by gold toning of the DAB product (not shown). ..

    Article Title: Severe acute respiratory syndrome coronavirus 3C‐like protease‐induced apoptosis
    Article Snippet: .. The apoptotic cells were further stained using a horseradish peroxidase/diaminobenzidine (HRP/DAB) system of the SuperPicTure™ Polymer Detection Kit (Zymed Laboratories Inc., San Francisco, CA). .. The percentage of apoptotic cells with a permanent intense brown deposit was counted at × 200 magnification using bright field microscopy.

    Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins
    Article Snippet: .. For immunostaining, cells at about 100% confluency were fixed with 2% paraformaldehyde-0.2% glutaraldehyde and stained with anti-myc (1:1,000) followed by anti-mouse secondary antibody and diaminobenzidine substrate (Lifetech). .. For FACS of transiently expressed CB5, A7 monolayers were transfected with pB5myc or vector only.

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    Thermo Fisher 3 3 diaminobenzidine dab ihc staining
    Representative <t>3,3-diaminobenzidine</t> <t>immunohistochemical</t> stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.
    3 3 Diaminobenzidine Dab Ihc Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab ihc staining/product/Thermo Fisher
    Average 99 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab ihc staining - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.

    Journal: Frontiers in Surgery

    Article Title: Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin–Angiotensin System

    doi: 10.3389/fsurg.2016.00051

    Figure Lengend Snippet: Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.

    Article Snippet: 3,3-Diaminobenzidine (DAB) IHC staining for SOX2 (1:500; cat# PA094, Thermo Fisher, Scientific, Scoresby, VIC, Australia), PRR (1:2000; cat# ab40790, Abcam, Cambridge, UK), ATIIR1 (1:30; cat# ab9391, Abcam), ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, LLC, Littleton, CO, USA), ACE (1:100; cat# MCA2054, AbD Serotec, Kidlington, UK) diluted with Bond™ primary antibody diluent (cat# AR9352, Leica) was done for all tissue samples.

    Techniques: Immunohistochemistry, Staining, Expressing

    Cellular Organization of the Adult Thoracic NG (A–C) Adult VNCs immunostained with anti-BRP (neuropil marker, blue) (A1–C) and anti-Dll (NG marker, green) (B) with astrocytes expressing mCD8::GFP (membrane marker, green) (A1 and A2), H2B::RFP (nuclear marker, red) (B) or the multicolor system FB1.1 (single-cell marker, red, yellow, and green) (C) under the control of alrm-Gal4 . (B) Astrocyte (H2B::RFP + , Dll + ): arrow, EG (Dll + ): arrowhead. . ) (F) under the control of R56F03-Gal4 . (E) Astrocyte: arrow (Dll + ), EG (H2B::RFP + , Dll + ): arrowhead. (D) arrow: in more than half of the samples analyzed (n = 10) a cell body of an EG (GFP + , Dll + ) was observed inside the neuropil, next to axon bundles. . (G and H) Prothoracic neuromeres with EG-expressing mCD4::GFP and immunostained with anti-BRP (blue) and anti-Elav (neuron marker, red) (G) or anti-NCad (neuropil marker, red) and Nrg (axon marker, blue) (H). (G) Asterisk: Elav + . (I and J) Prothoracic neuromeres with EG-expressing mCD4::GFP (I) and GFP::mCD8::HRP (J) immunostained with anti-BRP (blue) (I) or labeled with DAB (brown) (J). (K) Low-magnification electron microscope image of the boxed region in (J). (L–O) Enlargement of the boxed regions in (K). (L2) Enlargement of the boxed region in (L1). Note: (L1) and (L2) show the organization of the EG processes around the neuropil while (M)–(O) show the EG processes inside the neuropil. Pink lines outline axons. PG, perineurial glia; SPG, subperineurial glia; NG, neuropil glia; C, cortex; Np, neuropil; NL, neural lamella. (P) Bottom, schematic of adult CNS (blue: neuropils, gray: cortex); top, single astrocyte and EG labeled with mCD8::GFP under the control of alrm-Gal4 and R56F03-Gal4 using MARCM. Axes: green (posterior), blue (dorsal), red (medial). (Q) Average number of NG in the adult VNC, in which EG or astrocytes were expressing H2B::RFP under the control of R56F03-Gal4 or alrm-Gal4 , respectively, and immunostained with anti-Dll. Number of samples = 4/genotype. Error bars indicate SD. ProNm, Prothoracic neuromere; AMesoNm, Accessory mesothoracic neuromere; MesoNm, Mesothoracic neuromere; MetaNm, Metathoracic neuromere; ANm, Abdominal neuromeres; FS, frontal cross-section; TS, transverse cross-section; 3D, 3-dimensional reconstruction of confocal image stack; pMP, partial maximum projection.

    Journal: Neuron

    Article Title: Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila

    doi: 10.1016/j.neuron.2018.01.007

    Figure Lengend Snippet: Cellular Organization of the Adult Thoracic NG (A–C) Adult VNCs immunostained with anti-BRP (neuropil marker, blue) (A1–C) and anti-Dll (NG marker, green) (B) with astrocytes expressing mCD8::GFP (membrane marker, green) (A1 and A2), H2B::RFP (nuclear marker, red) (B) or the multicolor system FB1.1 (single-cell marker, red, yellow, and green) (C) under the control of alrm-Gal4 . (B) Astrocyte (H2B::RFP + , Dll + ): arrow, EG (Dll + ): arrowhead. . ) (F) under the control of R56F03-Gal4 . (E) Astrocyte: arrow (Dll + ), EG (H2B::RFP + , Dll + ): arrowhead. (D) arrow: in more than half of the samples analyzed (n = 10) a cell body of an EG (GFP + , Dll + ) was observed inside the neuropil, next to axon bundles. . (G and H) Prothoracic neuromeres with EG-expressing mCD4::GFP and immunostained with anti-BRP (blue) and anti-Elav (neuron marker, red) (G) or anti-NCad (neuropil marker, red) and Nrg (axon marker, blue) (H). (G) Asterisk: Elav + . (I and J) Prothoracic neuromeres with EG-expressing mCD4::GFP (I) and GFP::mCD8::HRP (J) immunostained with anti-BRP (blue) (I) or labeled with DAB (brown) (J). (K) Low-magnification electron microscope image of the boxed region in (J). (L–O) Enlargement of the boxed regions in (K). (L2) Enlargement of the boxed region in (L1). Note: (L1) and (L2) show the organization of the EG processes around the neuropil while (M)–(O) show the EG processes inside the neuropil. Pink lines outline axons. PG, perineurial glia; SPG, subperineurial glia; NG, neuropil glia; C, cortex; Np, neuropil; NL, neural lamella. (P) Bottom, schematic of adult CNS (blue: neuropils, gray: cortex); top, single astrocyte and EG labeled with mCD8::GFP under the control of alrm-Gal4 and R56F03-Gal4 using MARCM. Axes: green (posterior), blue (dorsal), red (medial). (Q) Average number of NG in the adult VNC, in which EG or astrocytes were expressing H2B::RFP under the control of R56F03-Gal4 or alrm-Gal4 , respectively, and immunostained with anti-Dll. Number of samples = 4/genotype. Error bars indicate SD. ProNm, Prothoracic neuromere; AMesoNm, Accessory mesothoracic neuromere; MesoNm, Mesothoracic neuromere; MetaNm, Metathoracic neuromere; ANm, Abdominal neuromeres; FS, frontal cross-section; TS, transverse cross-section; 3D, 3-dimensional reconstruction of confocal image stack; pMP, partial maximum projection.

    Article Snippet: HRP expression was revealed overnight using the DAB substrate kit (Thermofisher).

    Techniques: Marker, Expressing, Labeling, Microscopy

    The lack of Nox1 reduces fibrosis in CP. Pancreatic tissue were fixed with 10% formalin and embedded in paraffin. Following incubation with antibody against α-SMA, positive cells were visualized using 3, 3-diaminobenzi-dine tetrahydrochloride (DAB) as a chromogen (color: brown). (Arrows: α-SMA). Total magnification: 200X. n: 3.

    Journal: Free radical biology & medicine

    Article Title: NADPH oxidase 1 mediates caerulein-induced pancreatic fibrosis in chronic pancreatitis

    doi: 10.1016/j.freeradbiomed.2019.11.034

    Figure Lengend Snippet: The lack of Nox1 reduces fibrosis in CP. Pancreatic tissue were fixed with 10% formalin and embedded in paraffin. Following incubation with antibody against α-SMA, positive cells were visualized using 3, 3-diaminobenzi-dine tetrahydrochloride (DAB) as a chromogen (color: brown). (Arrows: α-SMA). Total magnification: 200X. n: 3.

    Article Snippet: IHC for α-SMA, NF-ĸB and p-AKT in whole pancreas: Cells positive for these proteins were determined using 3,3-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (color: brown).

    Techniques: Incubation

    Cleavage of caspase 3 (A) and 8 (B) in pancreatic cancer cell lines following rfhSP-D treatment. Pancreatic cancer cell lines were analyzed for caspase 8 and 3 activation via western blot using anti-rabbit cleaved caspase 3 and 8 (1:1,000) at 4°C overnight, followed by incubation with secondary anti-rabbit IgG HRP-conjugate (1:1,000) for 1 h at room temperature. The membrane was washed with PBST (PBS + 0.05% Tween 20) three times, 10 min each between each step. The bands were developed using 3,3′-diaminobenzidine substrate kit. The cleaved caspase 3 and 8 were detected only in the rfhSP-D treated samples of all cell lines, whereas no bands appeared in the untreated cell samples. Full-length caspase 8 bands are visible around 43 kDa. (C) Anti-GAPDH was used as a loading control.

    Journal: Frontiers in Immunology

    Article Title: A Recombinant Fragment of Human Surfactant Protein D induces Apoptosis in Pancreatic Cancer Cell Lines via Fas-Mediated Pathway

    doi: 10.3389/fimmu.2018.01126

    Figure Lengend Snippet: Cleavage of caspase 3 (A) and 8 (B) in pancreatic cancer cell lines following rfhSP-D treatment. Pancreatic cancer cell lines were analyzed for caspase 8 and 3 activation via western blot using anti-rabbit cleaved caspase 3 and 8 (1:1,000) at 4°C overnight, followed by incubation with secondary anti-rabbit IgG HRP-conjugate (1:1,000) for 1 h at room temperature. The membrane was washed with PBST (PBS + 0.05% Tween 20) three times, 10 min each between each step. The bands were developed using 3,3′-diaminobenzidine substrate kit. The cleaved caspase 3 and 8 were detected only in the rfhSP-D treated samples of all cell lines, whereas no bands appeared in the untreated cell samples. Full-length caspase 8 bands are visible around 43 kDa. (C) Anti-GAPDH was used as a loading control.

    Article Snippet: The color was developed using 3,3′-diaminobenzidine substrate kit (Thermo Fisher).

    Techniques: Activation Assay, Western Blot, Incubation