3 diaminobenzidine tetrahydrochloride  (Thermo Fisher)


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    Thermo Fisher 3 diaminobenzidine tetrahydrochloride
    3 Diaminobenzidine Tetrahydrochloride, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2020-11
    99/100 stars

    Related Products / Commonly Used Together

    pbs
    substrate chromogen solution
    tris-cl
    tbst
    peroxidase activity
    dpx
    biotinylated secondary antibody
    horseradish peroxidase
    chromogen 3
    anti-mouse igg-horse radish peroxidase hrp conjugate
    cyt c
    tris buffer
    avidin-biotin complex
    secondary antibody
    polymer-based detection system

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: NADPH oxidase 1 mediates caerulein-induced pancreatic fibrosis in chronic pancreatitis
    Article Snippet: .. IHC for α-SMA, NF-ĸB and p-AKT in whole pancreas: Cells positive for these proteins were determined using 3,3-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (color: brown). .. Briefly, slides were incubated first with blocking buffer (3% BSA in PBS with 0.05% Tween-20) for 1 h at room temperature and then with antibody against α-SMA, NF-ĸB or p-AKT overnight at 4 °C.

    In Situ:

    Article Title: Stress Sensitivity Is Associated with Differential Accumulation of Reactive Oxygen and Nitrogen Species in Maize Genotypes with Contrasting Levels of Drought Tolerance
    Article Snippet: .. Hydrogen Peroxide Staining and Quantifica tion The in situ detection of hydrogen peroxide is conducted by staining with 3,3′-diaminobenzidine tetrahydrochloride hydrate (DAB) (AC11209-0050, ACROS Organics, Pittsburgh, PA, USA) using previously described methods with slight modification [ , , ]. .. For staining, 3–5 leaves from each time point were sampled and immediately placed in 15 mL falcon tubes.

    Blocking Assay:

    Article Title: Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats
    Article Snippet: .. To retrieve the antigen, slides were boiled with 10 mmol/l citrate buffer (pH 7) for 10 min. After preincubation with Ultra V block (Lab Vision) for 20 min, sections were incubated with the primary antibody for 1 hour at room temperature, followed sequentially by biotinylated goat antipolyvalen (Lab Vision) for 20 min and streptavidin peroxidase complex (Lab Vision) for 20 min. We used 3,3'-diaminobenzidine tetrahydrochloride/DAB (Lab Vision) as the chromagen, and hematoxylin for nuclear counterstain, and then rinsed and mounted. ..

    Purification:

    Article Title: Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultrabroad Dynamic Range
    Article Snippet: .. Next, the strip was immersed in another well for 5 min filled with 330 μL (enough solution to cover test line on strip in well) freshly prepared PtNC development solution containing a modified Pierce CN/DAB (4-chloro-1-naphthol/3,3′-diaminobenzidine, tetrahydrochloride) Substrate Kit (Thermo Scientific) adjusted with hydrogen peroxide solution 30% (w/w) (Sigma) to a final added peroxide concentration of 4 M. Finally, the strip was moved into a well containing 330 μL purified water for 1 min to stop the reaction. .. Strips were imaged with an iPhone 6 mobile phone camera for all experiments, except the clinical samples, which were imaged using a camera (Canon PowerShot G15) approved for use in contained environment for handling infectious samples.

    Concentration Assay:

    Article Title: Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultrabroad Dynamic Range
    Article Snippet: .. Next, the strip was immersed in another well for 5 min filled with 330 μL (enough solution to cover test line on strip in well) freshly prepared PtNC development solution containing a modified Pierce CN/DAB (4-chloro-1-naphthol/3,3′-diaminobenzidine, tetrahydrochloride) Substrate Kit (Thermo Scientific) adjusted with hydrogen peroxide solution 30% (w/w) (Sigma) to a final added peroxide concentration of 4 M. Finally, the strip was moved into a well containing 330 μL purified water for 1 min to stop the reaction. .. Strips were imaged with an iPhone 6 mobile phone camera for all experiments, except the clinical samples, which were imaged using a camera (Canon PowerShot G15) approved for use in contained environment for handling infectious samples.

    Incubation:

    Article Title: Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats
    Article Snippet: .. To retrieve the antigen, slides were boiled with 10 mmol/l citrate buffer (pH 7) for 10 min. After preincubation with Ultra V block (Lab Vision) for 20 min, sections were incubated with the primary antibody for 1 hour at room temperature, followed sequentially by biotinylated goat antipolyvalen (Lab Vision) for 20 min and streptavidin peroxidase complex (Lab Vision) for 20 min. We used 3,3'-diaminobenzidine tetrahydrochloride/DAB (Lab Vision) as the chromagen, and hematoxylin for nuclear counterstain, and then rinsed and mounted. ..

    Stripping Membranes:

    Article Title: Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultrabroad Dynamic Range
    Article Snippet: .. Next, the strip was immersed in another well for 5 min filled with 330 μL (enough solution to cover test line on strip in well) freshly prepared PtNC development solution containing a modified Pierce CN/DAB (4-chloro-1-naphthol/3,3′-diaminobenzidine, tetrahydrochloride) Substrate Kit (Thermo Scientific) adjusted with hydrogen peroxide solution 30% (w/w) (Sigma) to a final added peroxide concentration of 4 M. Finally, the strip was moved into a well containing 330 μL purified water for 1 min to stop the reaction. .. Strips were imaged with an iPhone 6 mobile phone camera for all experiments, except the clinical samples, which were imaged using a camera (Canon PowerShot G15) approved for use in contained environment for handling infectious samples.

    Modification:

    Article Title: Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultrabroad Dynamic Range
    Article Snippet: .. Next, the strip was immersed in another well for 5 min filled with 330 μL (enough solution to cover test line on strip in well) freshly prepared PtNC development solution containing a modified Pierce CN/DAB (4-chloro-1-naphthol/3,3′-diaminobenzidine, tetrahydrochloride) Substrate Kit (Thermo Scientific) adjusted with hydrogen peroxide solution 30% (w/w) (Sigma) to a final added peroxide concentration of 4 M. Finally, the strip was moved into a well containing 330 μL purified water for 1 min to stop the reaction. .. Strips were imaged with an iPhone 6 mobile phone camera for all experiments, except the clinical samples, which were imaged using a camera (Canon PowerShot G15) approved for use in contained environment for handling infectious samples.

    Article Title: Stress Sensitivity Is Associated with Differential Accumulation of Reactive Oxygen and Nitrogen Species in Maize Genotypes with Contrasting Levels of Drought Tolerance
    Article Snippet: .. Hydrogen Peroxide Staining and Quantifica tion The in situ detection of hydrogen peroxide is conducted by staining with 3,3′-diaminobenzidine tetrahydrochloride hydrate (DAB) (AC11209-0050, ACROS Organics, Pittsburgh, PA, USA) using previously described methods with slight modification [ , , ]. .. For staining, 3–5 leaves from each time point were sampled and immediately placed in 15 mL falcon tubes.

    Staining:

    Article Title: Stress Sensitivity Is Associated with Differential Accumulation of Reactive Oxygen and Nitrogen Species in Maize Genotypes with Contrasting Levels of Drought Tolerance
    Article Snippet: .. Hydrogen Peroxide Staining and Quantifica tion The in situ detection of hydrogen peroxide is conducted by staining with 3,3′-diaminobenzidine tetrahydrochloride hydrate (DAB) (AC11209-0050, ACROS Organics, Pittsburgh, PA, USA) using previously described methods with slight modification [ , , ]. .. For staining, 3–5 leaves from each time point were sampled and immediately placed in 15 mL falcon tubes.

    Article Title: Expression of zinc finger E-box-binding homeobox factor 1 in epithelial ovarian cancer: A clinicopathological analysis of 238 patients
    Article Snippet: .. The slides were subsequently stained with 3,3′-diaminobenzidine tetrahydrochloride for 10 min and counter-stained with hematoxylin, dehydrated, and mounted in Richard-Allan Scientific Cytoseal XYL (Thermo Fisher Scientific, Waltham, MA, USA). .. A known ZEB1-positive human esophageal carcinoma slide was used as positive control.

    Binding Assay:

    Article Title: Peri-conceptional changes in maternal exposure to sewage sludge chemicals disturbs fetal thyroid gland development in sheep
    Article Snippet: .. Specific binding was visualized using the 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate (Pierce Biotechnology, Rockford, IL). .. Nuclei were counterstained with hematoxylin and Ki67-positive and NIS-positive cells were imaged using a Zeiss Axio Imager A2 microscope and analysis was performed with the Zeiss Axiovision 4.8 software for male and female thyroids separately.

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    • 3 3 diaminobenzidine dab ihc staining  (Thermo Fisher)
    99
    Thermo Fisher 3 3 diaminobenzidine dab ihc staining
    Representative <t>3,3-diaminobenzidine</t> <t>immunohistochemical</t> stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.
    3 3 Diaminobenzidine Dab Ihc Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab ihc staining/product/Thermo Fisher
    Average 99 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab ihc staining - by Bioz Stars, 2020-11
    99/100 stars
    		  Buy from Supplier
    3 3 diaminobenzidine tetrahydrochloride dab  (Thermo Fisher)
    99
    Thermo Fisher 3 3 diaminobenzidine tetrahydrochloride dab
    The lack of Nox1 reduces fibrosis in CP. Pancreatic tissue were fixed with 10% formalin and embedded in paraffin. Following incubation with antibody against α-SMA, positive cells were visualized using 3, 3-diaminobenzi-dine <t>tetrahydrochloride</t> (DAB) as a chromogen (color: brown). (Arrows: α-SMA). Total magnification: 200X. n: 3.
    3 3 Diaminobenzidine Tetrahydrochloride Dab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride dab/product/Thermo Fisher
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine tetrahydrochloride dab - by Bioz Stars, 2020-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.

    Journal: Frontiers in Surgery

    Article Title: Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin–Angiotensin System

    doi: 10.3389/fsurg.2016.00051

    Figure Lengend Snippet: Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.

    Article Snippet: 3,3-Diaminobenzidine (DAB) IHC staining for SOX2 (1:500; cat# PA094, Thermo Fisher, Scientific, Scoresby, VIC, Australia), PRR (1:2000; cat# ab40790, Abcam, Cambridge, UK), ATIIR1 (1:30; cat# ab9391, Abcam), ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, LLC, Littleton, CO, USA), ACE (1:100; cat# MCA2054, AbD Serotec, Kidlington, UK) diluted with Bond™ primary antibody diluent (cat# AR9352, Leica) was done for all tissue samples.

    Techniques: Immunohistochemistry, Staining, Expressing

    Cellular Organization of the Adult Thoracic NG (A–C) Adult VNCs immunostained with anti-BRP (neuropil marker, blue) (A1–C) and anti-Dll (NG marker, green) (B) with astrocytes expressing mCD8::GFP (membrane marker, green) (A1 and A2), H2B::RFP (nuclear marker, red) (B) or the multicolor system FB1.1 (single-cell marker, red, yellow, and green) (C) under the control of alrm-Gal4 . (B) Astrocyte (H2B::RFP + , Dll + ): arrow, EG (Dll + ): arrowhead. . ) (F) under the control of R56F03-Gal4 . (E) Astrocyte: arrow (Dll + ), EG (H2B::RFP + , Dll + ): arrowhead. (D) arrow: in more than half of the samples analyzed (n = 10) a cell body of an EG (GFP + , Dll + ) was observed inside the neuropil, next to axon bundles. . (G and H) Prothoracic neuromeres with EG-expressing mCD4::GFP and immunostained with anti-BRP (blue) and anti-Elav (neuron marker, red) (G) or anti-NCad (neuropil marker, red) and Nrg (axon marker, blue) (H). (G) Asterisk: Elav + . (I and J) Prothoracic neuromeres with EG-expressing mCD4::GFP (I) and GFP::mCD8::HRP (J) immunostained with anti-BRP (blue) (I) or labeled with DAB (brown) (J). (K) Low-magnification electron microscope image of the boxed region in (J). (L–O) Enlargement of the boxed regions in (K). (L2) Enlargement of the boxed region in (L1). Note: (L1) and (L2) show the organization of the EG processes around the neuropil while (M)–(O) show the EG processes inside the neuropil. Pink lines outline axons. PG, perineurial glia; SPG, subperineurial glia; NG, neuropil glia; C, cortex; Np, neuropil; NL, neural lamella. (P) Bottom, schematic of adult CNS (blue: neuropils, gray: cortex); top, single astrocyte and EG labeled with mCD8::GFP under the control of alrm-Gal4 and R56F03-Gal4 using MARCM. Axes: green (posterior), blue (dorsal), red (medial). (Q) Average number of NG in the adult VNC, in which EG or astrocytes were expressing H2B::RFP under the control of R56F03-Gal4 or alrm-Gal4 , respectively, and immunostained with anti-Dll. Number of samples = 4/genotype. Error bars indicate SD. ProNm, Prothoracic neuromere; AMesoNm, Accessory mesothoracic neuromere; MesoNm, Mesothoracic neuromere; MetaNm, Metathoracic neuromere; ANm, Abdominal neuromeres; FS, frontal cross-section; TS, transverse cross-section; 3D, 3-dimensional reconstruction of confocal image stack; pMP, partial maximum projection.

    Journal: Neuron

    Article Title: Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila

    doi: 10.1016/j.neuron.2018.01.007

    Figure Lengend Snippet: Cellular Organization of the Adult Thoracic NG (A–C) Adult VNCs immunostained with anti-BRP (neuropil marker, blue) (A1–C) and anti-Dll (NG marker, green) (B) with astrocytes expressing mCD8::GFP (membrane marker, green) (A1 and A2), H2B::RFP (nuclear marker, red) (B) or the multicolor system FB1.1 (single-cell marker, red, yellow, and green) (C) under the control of alrm-Gal4 . (B) Astrocyte (H2B::RFP + , Dll + ): arrow, EG (Dll + ): arrowhead. . ) (F) under the control of R56F03-Gal4 . (E) Astrocyte: arrow (Dll + ), EG (H2B::RFP + , Dll + ): arrowhead. (D) arrow: in more than half of the samples analyzed (n = 10) a cell body of an EG (GFP + , Dll + ) was observed inside the neuropil, next to axon bundles. . (G and H) Prothoracic neuromeres with EG-expressing mCD4::GFP and immunostained with anti-BRP (blue) and anti-Elav (neuron marker, red) (G) or anti-NCad (neuropil marker, red) and Nrg (axon marker, blue) (H). (G) Asterisk: Elav + . (I and J) Prothoracic neuromeres with EG-expressing mCD4::GFP (I) and GFP::mCD8::HRP (J) immunostained with anti-BRP (blue) (I) or labeled with DAB (brown) (J). (K) Low-magnification electron microscope image of the boxed region in (J). (L–O) Enlargement of the boxed regions in (K). (L2) Enlargement of the boxed region in (L1). Note: (L1) and (L2) show the organization of the EG processes around the neuropil while (M)–(O) show the EG processes inside the neuropil. Pink lines outline axons. PG, perineurial glia; SPG, subperineurial glia; NG, neuropil glia; C, cortex; Np, neuropil; NL, neural lamella. (P) Bottom, schematic of adult CNS (blue: neuropils, gray: cortex); top, single astrocyte and EG labeled with mCD8::GFP under the control of alrm-Gal4 and R56F03-Gal4 using MARCM. Axes: green (posterior), blue (dorsal), red (medial). (Q) Average number of NG in the adult VNC, in which EG or astrocytes were expressing H2B::RFP under the control of R56F03-Gal4 or alrm-Gal4 , respectively, and immunostained with anti-Dll. Number of samples = 4/genotype. Error bars indicate SD. ProNm, Prothoracic neuromere; AMesoNm, Accessory mesothoracic neuromere; MesoNm, Mesothoracic neuromere; MetaNm, Metathoracic neuromere; ANm, Abdominal neuromeres; FS, frontal cross-section; TS, transverse cross-section; 3D, 3-dimensional reconstruction of confocal image stack; pMP, partial maximum projection.

    Article Snippet: HRP expression was revealed overnight using the DAB substrate kit (Thermofisher).

    Techniques: Marker, Expressing, Labeling, Microscopy

    Cellular organization of the adult thoracic NG. (A-C): Adult VNSs immunostained with anti-BRP (neuropil marker, blue) (A1-C4) and anti-Dll (NG marker, red) (B1-B3) with astrocytes expressing mCD8::GFP (membrane marker, green) (A1-C4), H2B::RFP (Nuclear marker, red) (B1-B3) or the multicolor system FB1.1 (single cell marker, red, yellow and green) (C1-C4) under the control of alrm-Gal4 . (B1-B3) , astrocyte (H2B::RFP+, Dll+): arrow, EG (Dll+): arrowhead. (C1-C4) Different astrocytes with processes occupying different neuropil territories: arrows. See also Figure S1 and video 1. (D-F): Adult VNSs immunostained with anti-BRP (neuropil marker, blue) (D1-F4) and anti-Dll (NG marker, red) ( E1-E3) with EG expressing mCD4::GFP (membrane marker, green) (D1-D3), H2B::RFP (nuclear marker, red) (E1-E3) or the multicolor system FB1.1 (single cell marker, red, yellow and green; ( Hadjieconomou et al., 2011 )) (F1-F4) under the control of R56F03-Gal4 . (E1-E3), astrocyte: arrow (Dll+), EG (H2B::RFP+, Dll+): arrowhead. (F1-F4) Different EG with processes occupying different neuropil territories: arrows. See also Figure S1 and video 1. (G-I): Prothoracic neuromeres with EG expressing mCD4::GFP and immunostained with anti-BRP (blue) and anti-Elav (neuron marker, red) (G) , anti-BRP (blue) and Dll (red) (H) or anti-NCad (neuropil marker, red) and Nrg (axon marker, blue) (I1-2) . (G) Asterisk: Elav+ neuron wrapped by an EG. (H) arrow: in more than half of the samples analyzed (N=10) a cell body of an EG (GFP +, Dll+) was observed inside the neuropil, next to axon bundles. Enlargements of the boxed regions are to the right of each panel. See also video 2. (J-K): Prothoracic neuromeres with EG expressing mCD4::GFP (J) and GFP::mCD8::HRP (K) immunostained with anti-BRP (blue) (J) or labeled with DAB (Black) (K) . (L) : Low magnification electron microscopy image of the boxed region in (K) . (M-P): enlargement of the boxed regions in (L) . PG: perineurial glia. SPG: subperineurial glia, NG: Neuropil Glia, C: Cortex, Np: Neuropil, NL: neural lamella. (O) : Left, schematic of adult CNS (blue: neuropils, grey: cortex); right, an individual astrocyte and EG labeled with mCD8::GFP under the control of alrm-Gal4 and R56F03-Gal4 using the MARCM technique. Axes: green (posterior), blue (dorsal), red (medial). (R): Average number of NG in the adult VNS, in which EG or astrocytes were expressing H2B::RFP under the control of R56F03-Gal4 or alrm-Gal4 , respectively, and immunostained with anti-Dll. Number of samples = 4/genotype. Error bars indicate standard deviation. ProNm: Prothoracic neuromere, AMesoNm: Accessory mesothoracic neuromere, MesoNm: Mesothoracic neuromere, MetaNm: Metathoracic neuromere, ANm: Abdominal neuromeres; FS: frontal cross section, TS: transverse cross section, 3D: 3 dimensional reconstruction of confocal image stack. pMP: partial maximum projection.

    Journal: bioRxiv

    Article Title: Differing strategies used by motor neurons and glia to achieve robust development of an adult neuropil in Drosophila

    doi: 10.1101/149229

    Figure Lengend Snippet: Cellular organization of the adult thoracic NG. (A-C): Adult VNSs immunostained with anti-BRP (neuropil marker, blue) (A1-C4) and anti-Dll (NG marker, red) (B1-B3) with astrocytes expressing mCD8::GFP (membrane marker, green) (A1-C4), H2B::RFP (Nuclear marker, red) (B1-B3) or the multicolor system FB1.1 (single cell marker, red, yellow and green) (C1-C4) under the control of alrm-Gal4 . (B1-B3) , astrocyte (H2B::RFP+, Dll+): arrow, EG (Dll+): arrowhead. (C1-C4) Different astrocytes with processes occupying different neuropil territories: arrows. See also Figure S1 and video 1. (D-F): Adult VNSs immunostained with anti-BRP (neuropil marker, blue) (D1-F4) and anti-Dll (NG marker, red) ( E1-E3) with EG expressing mCD4::GFP (membrane marker, green) (D1-D3), H2B::RFP (nuclear marker, red) (E1-E3) or the multicolor system FB1.1 (single cell marker, red, yellow and green; ( Hadjieconomou et al., 2011 )) (F1-F4) under the control of R56F03-Gal4 . (E1-E3), astrocyte: arrow (Dll+), EG (H2B::RFP+, Dll+): arrowhead. (F1-F4) Different EG with processes occupying different neuropil territories: arrows. See also Figure S1 and video 1. (G-I): Prothoracic neuromeres with EG expressing mCD4::GFP and immunostained with anti-BRP (blue) and anti-Elav (neuron marker, red) (G) , anti-BRP (blue) and Dll (red) (H) or anti-NCad (neuropil marker, red) and Nrg (axon marker, blue) (I1-2) . (G) Asterisk: Elav+ neuron wrapped by an EG. (H) arrow: in more than half of the samples analyzed (N=10) a cell body of an EG (GFP +, Dll+) was observed inside the neuropil, next to axon bundles. Enlargements of the boxed regions are to the right of each panel. See also video 2. (J-K): Prothoracic neuromeres with EG expressing mCD4::GFP (J) and GFP::mCD8::HRP (K) immunostained with anti-BRP (blue) (J) or labeled with DAB (Black) (K) . (L) : Low magnification electron microscopy image of the boxed region in (K) . (M-P): enlargement of the boxed regions in (L) . PG: perineurial glia. SPG: subperineurial glia, NG: Neuropil Glia, C: Cortex, Np: Neuropil, NL: neural lamella. (O) : Left, schematic of adult CNS (blue: neuropils, grey: cortex); right, an individual astrocyte and EG labeled with mCD8::GFP under the control of alrm-Gal4 and R56F03-Gal4 using the MARCM technique. Axes: green (posterior), blue (dorsal), red (medial). (R): Average number of NG in the adult VNS, in which EG or astrocytes were expressing H2B::RFP under the control of R56F03-Gal4 or alrm-Gal4 , respectively, and immunostained with anti-Dll. Number of samples = 4/genotype. Error bars indicate standard deviation. ProNm: Prothoracic neuromere, AMesoNm: Accessory mesothoracic neuromere, MesoNm: Mesothoracic neuromere, MetaNm: Metathoracic neuromere, ANm: Abdominal neuromeres; FS: frontal cross section, TS: transverse cross section, 3D: 3 dimensional reconstruction of confocal image stack. pMP: partial maximum projection.

    Article Snippet: The expression of the HRP (Horseradish Peroxydase) were revealed overnight using the DAB substrate kit from Thermofisher.

    Techniques: Marker, Expressing, Labeling, Electron Microscopy, Standard Deviation

    The lack of Nox1 reduces fibrosis in CP. Pancreatic tissue were fixed with 10% formalin and embedded in paraffin. Following incubation with antibody against α-SMA, positive cells were visualized using 3, 3-diaminobenzi-dine tetrahydrochloride (DAB) as a chromogen (color: brown). (Arrows: α-SMA). Total magnification: 200X. n: 3.

    Journal: Free radical biology & medicine

    Article Title: NADPH oxidase 1 mediates caerulein-induced pancreatic fibrosis in chronic pancreatitis

    doi: 10.1016/j.freeradbiomed.2019.11.034

    Figure Lengend Snippet: The lack of Nox1 reduces fibrosis in CP. Pancreatic tissue were fixed with 10% formalin and embedded in paraffin. Following incubation with antibody against α-SMA, positive cells were visualized using 3, 3-diaminobenzi-dine tetrahydrochloride (DAB) as a chromogen (color: brown). (Arrows: α-SMA). Total magnification: 200X. n: 3.

    Article Snippet: IHC for α-SMA, NF-ĸB and p-AKT in whole pancreas: Cells positive for these proteins were determined using 3,3-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (color: brown).

    Techniques: Incubation