3 diaminobenzidine tetrahydrochloride (Thermo Fisher)
99
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Name:
DAB Substrate Powder 3 3 diaminobenzidine tetrahydrochloride
Description:
Thermo Scientific Pierce DAB enables chromogenic detection of horseradish peroxidase HRP activity based the action of 3 3 diaminobenzidine DAB in Western blot and tissue staining methods DAB 3 3 diaminobenzidine tetrahydrochloride MW 214 1 reacts with HRP in the presence of peroxide to yield an insoluble brown colored product at locations where peroxidase conjugated antibodies are bound to samples The brown precipitate is insoluble in alcohol and other organic solvents making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media Features of DAB Powder • HRP substrate for detection of horseradish peroxidase activity on solid media including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic no special equipment needed for visualization the DAB reaction produces brown bands or spots at sites of reaction with HRP conjugated antibodies or probes • Package options choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use DAB has been used in a variety of applications including Western blotting immunohistochemistry and electron microscopy Many enhancement methods have been reported for DAB involving different buffer conditions the addition of metal ions and post treatment applications Ref 1 7 also see the Pierce Metal Enhanced DAB Substrate Kit Part No 34065 One disadvantage of DAB compared to other substrates is the need to use the working solution immediately because it begins a reaction process upon the addition of hydrogen peroxide This results in increased background color of the substrate However the brown bands resulting from DAB reactions do not fade as quickly as the color products of other precipitating HRP substrates Related Products Pierce DAB Substrate Kit
Catalog Number:
34001
Price:
None
Applications:
Cell Analysis|Cellular Imaging|IHC Staining & Detection|Immunohistochemistry (IHC)|Protein Assays and Analysis|Protein Biology|Western Blotting
Category:
Labeling Detection Products
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Structured Review
Thermo Fisher
3 diaminobenzidine tetrahydrochloride
Thermo Scientific Pierce DAB enables chromogenic detection of horseradish peroxidase HRP activity based the action of 3 3 diaminobenzidine DAB in Western blot and tissue staining methods DAB 3 3 diaminobenzidine tetrahydrochloride MW 214 1 reacts with HRP in the presence of peroxide to yield an insoluble brown colored product at locations where peroxidase conjugated antibodies are bound to samples The brown precipitate is insoluble in alcohol and other organic solvents making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media Features of DAB Powder • HRP substrate for detection of horseradish peroxidase activity on solid media including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic no special equipment needed for visualization the DAB reaction produces brown bands or spots at sites of reaction with HRP conjugated antibodies or probes • Package options choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use DAB has been used in a variety of applications including Western blotting immunohistochemistry and electron microscopy Many enhancement methods have been reported for DAB involving different buffer conditions the addition of metal ions and post treatment applications Ref 1 7 also see the Pierce Metal Enhanced DAB Substrate Kit Part No 34065 One disadvantage of DAB compared to other substrates is the need to use the working solution immediately because it begins a reaction process upon the addition of hydrogen peroxide This results in increased background color of the substrate However the brown bands resulting from DAB reactions do not fade as quickly as the color products of other precipitating HRP substrates Related Products Pierce DAB Substrate Kit
https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Thermo Fisher
Average 99 stars, based on 9 article reviews
Price from $9.99 to $1999.99
Thermo Scientific Pierce DAB enables chromogenic detection of horseradish peroxidase HRP activity based the action of 3 3 diaminobenzidine DAB in Western blot and tissue staining methods DAB 3 3 diaminobenzidine tetrahydrochloride MW 214 1 reacts with HRP in the presence of peroxide to yield an insoluble brown colored product at locations where peroxidase conjugated antibodies are bound to samples The brown precipitate is insoluble in alcohol and other organic solvents making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media Features of DAB Powder • HRP substrate for detection of horseradish peroxidase activity on solid media including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic no special equipment needed for visualization the DAB reaction produces brown bands or spots at sites of reaction with HRP conjugated antibodies or probes • Package options choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use DAB has been used in a variety of applications including Western blotting immunohistochemistry and electron microscopy Many enhancement methods have been reported for DAB involving different buffer conditions the addition of metal ions and post treatment applications Ref 1 7 also see the Pierce Metal Enhanced DAB Substrate Kit Part No 34065 One disadvantage of DAB compared to other substrates is the need to use the working solution immediately because it begins a reaction process upon the addition of hydrogen peroxide This results in increased background color of the substrate However the brown bands resulting from DAB reactions do not fade as quickly as the color products of other precipitating HRP substrates Related Products Pierce DAB Substrate Kit
https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Thermo Fisher
Average 99 stars, based on 9 article reviews
Price from $9.99 to $1999.99
3 diaminobenzidine tetrahydrochloride - by Bioz Stars,
2021-02
99/100 stars
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![Representative <t>3,3-diaminobenzidine</t> <t>immunohistochemical</t> stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.](https://storage.googleapis.com/bioz_article_images/PMC5037176/fsurg-03-00051-g001.jpg)
