3 diaminobenzidine tetrahydrochloride (Agilent technologies)
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3 Diaminobenzidine Tetrahydrochloride, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 61 article reviews
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Images
1) Product Images from "PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry"
Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry
Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
doi: 10.1089/mab.2017.0008

Figure Legend Snippet: Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.
Techniques Used: Immunohistochemistry, Incubation, Staining

Figure Legend Snippet: Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.
Techniques Used: Immunohistochemistry, Incubation, Staining
2) Product Images from "KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association"
Article Title: KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association
Journal: Molecular Vision
doi:

Figure Legend Snippet: KIF14 and E2F3 expression in tumor and control retina. A and D show no immunoreactivity of KIF14 and E2F3 in non-neoplastic retina. B and C show positive nucleocytoplasm (arrow heads) and nuclear (arrows) expression of KIF14 in tumor cells. E and F show positive nuclear (arrows) expression of E2F3 in tumor cells. All are 3,3′-diaminobenzidine staining with hematoxylin counterstain. The pictures were taken under 40X magnification.
Techniques Used: Expressing, Staining
3) Product Images from "A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin"
Article Title: A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin
Journal: Scientific Reports
doi: 10.1038/srep05924

Figure Legend Snippet: Podoplanin protein expression was determined immunohist ochemically in paraffin-embedded tumor specimens. Histologic sections 4 µm thick were deparaffinized in xylene and rehydrated, then autoclaved in citrate buffer (pH 6.0) for 20 min. Sections were incubated with 5 μg/ml of LpMab-2 (a-e) or LpMab-7 (f–j) overnight at 4°C with subsequent treatment using an LSAB kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB) for 10 min and was counterstained with hematoxylin. (a, f) Esophageal squamous cell carcinomas include both cancer cells (upper) and lymphatic endothelial cells (lower). Cancer cells were stained with both LpMab-7 (f) and LpMab-2 (a), whereas lymphatic endothelial cells were stained with LpMab-7 (f), not with LpMab-2 (a). (b, g) Seminoma includes both cancer cells (left) and lymphatic endothelial cells (right, upper). Cancer cells were stained with both LpMab-7 (g) and LpMab-2 (b), whereas lymphatic endothelial cells were stained with LpMab-7 (g), not with LpMab-2 (b). (c, h) Glioblastomas were stained with both LpMab-7 (h) and LpMab-2 (c). (d, i) Esophageal lymphatic endothelial cells were stained with LpMab-7 (i), not with LpMab-2 (d). Lung type I alveolar cells were stained with LpMab-7 (j), not with LpMab-2 (e). Arrows indicate lymphatic endothelial cells (f, g, i).
Techniques Used: Expressing, Incubation, Staining
4) Product Images from "Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN"
Article Title: Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN
Journal: Cancer Medicine
doi: 10.1002/cam4.954

Figure Legend Snippet: Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in human cancer tissues. Tissue sections were prepared from the human cancer tissues as follows: glioblastoma ( GBM , A and E); oral squamous cell carcinoma ( OSCC ; B, D, F, and H); seminoma ( SE , C and G). Sections were incubated (antigen retrieval omitted) with 1 μ g/mL of LpMab‐21 (A–D), reacted with the Envision+ kit, color was developed using DAB , and samples were then counterstained with hematoxylin. Sections were stained with hematoxylin and eosin as well (E–H). Arrowheads: lymphatic endothelial cells. Scale bar = 100 μ m. DAB, 3‐diaminobenzidine tetrahydrochloride
Techniques Used: Immunohistochemistry, Expressing, Incubation, Staining

Figure Legend Snippet: Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in normal human tissues. Tissues harvested from the esophagus (A), breast (B), colon (C), lung (D), kidney (E), and rectum (F). After antigen retrieval procedure, sections were incubated with 1 μ g/mL of LpMab‐21, reacted with the Envision+ kit, color was developed, using DAB , and samples were then counterstained with hematoxylin. Arrowheads, lymphatic endothelial cells. Scale bar , 100 μ m. 3, DAB, 3‐diaminobenzidine tetrahydrochloride.
Techniques Used: Immunohistochemistry, Expressing, Incubation
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