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Agilent technologies 3 diaminobenzidine tetrahydrochloride
Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, <t>3-diaminobenzidine</t> <t>tetrahydrochloride,</t> and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.
3 Diaminobenzidine Tetrahydrochloride, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Agilent technologies
Average 92 stars, based on 61 article reviews
Price from $9.99 to $1999.99
3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2020-11
92/100 stars

Images

1) Product Images from "PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry"

Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

doi: 10.1089/mab.2017.0008

Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.
Figure Legend Snippet: Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

Techniques Used: Immunohistochemistry, Incubation, Staining

Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.
Figure Legend Snippet: Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

Techniques Used: Immunohistochemistry, Incubation, Staining

2) Product Images from "KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association"

Article Title: KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association

Journal: Molecular Vision

doi:

KIF14 and E2F3 expression in tumor and control retina. A and D show no immunoreactivity of KIF14 and E2F3 in non-neoplastic retina. B and C show positive nucleocytoplasm (arrow heads) and nuclear (arrows) expression of KIF14 in tumor cells. E and F show positive nuclear (arrows) expression of E2F3 in tumor cells. All are 3,3′-diaminobenzidine staining with hematoxylin counterstain. The pictures were taken under 40X magnification.
Figure Legend Snippet: KIF14 and E2F3 expression in tumor and control retina. A and D show no immunoreactivity of KIF14 and E2F3 in non-neoplastic retina. B and C show positive nucleocytoplasm (arrow heads) and nuclear (arrows) expression of KIF14 in tumor cells. E and F show positive nuclear (arrows) expression of E2F3 in tumor cells. All are 3,3′-diaminobenzidine staining with hematoxylin counterstain. The pictures were taken under 40X magnification.

Techniques Used: Expressing, Staining

3) Product Images from "A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin"

Article Title: A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin

Journal: Scientific Reports

doi: 10.1038/srep05924

Podoplanin protein expression was determined immunohist ochemically in paraffin-embedded tumor specimens. Histologic sections 4 µm thick were deparaffinized in xylene and rehydrated, then autoclaved in citrate buffer (pH 6.0) for 20 min. Sections were incubated with 5 μg/ml of LpMab-2 (a-e) or LpMab-7 (f–j) overnight at 4°C with subsequent treatment using an LSAB kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB) for 10 min and was counterstained with hematoxylin. (a, f) Esophageal squamous cell carcinomas include both cancer cells (upper) and lymphatic endothelial cells (lower). Cancer cells were stained with both LpMab-7 (f) and LpMab-2 (a), whereas lymphatic endothelial cells were stained with LpMab-7 (f), not with LpMab-2 (a). (b, g) Seminoma includes both cancer cells (left) and lymphatic endothelial cells (right, upper). Cancer cells were stained with both LpMab-7 (g) and LpMab-2 (b), whereas lymphatic endothelial cells were stained with LpMab-7 (g), not with LpMab-2 (b). (c, h) Glioblastomas were stained with both LpMab-7 (h) and LpMab-2 (c). (d, i) Esophageal lymphatic endothelial cells were stained with LpMab-7 (i), not with LpMab-2 (d). Lung type I alveolar cells were stained with LpMab-7 (j), not with LpMab-2 (e). Arrows indicate lymphatic endothelial cells (f, g, i).
Figure Legend Snippet: Podoplanin protein expression was determined immunohist ochemically in paraffin-embedded tumor specimens. Histologic sections 4 µm thick were deparaffinized in xylene and rehydrated, then autoclaved in citrate buffer (pH 6.0) for 20 min. Sections were incubated with 5 μg/ml of LpMab-2 (a-e) or LpMab-7 (f–j) overnight at 4°C with subsequent treatment using an LSAB kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB) for 10 min and was counterstained with hematoxylin. (a, f) Esophageal squamous cell carcinomas include both cancer cells (upper) and lymphatic endothelial cells (lower). Cancer cells were stained with both LpMab-7 (f) and LpMab-2 (a), whereas lymphatic endothelial cells were stained with LpMab-7 (f), not with LpMab-2 (a). (b, g) Seminoma includes both cancer cells (left) and lymphatic endothelial cells (right, upper). Cancer cells were stained with both LpMab-7 (g) and LpMab-2 (b), whereas lymphatic endothelial cells were stained with LpMab-7 (g), not with LpMab-2 (b). (c, h) Glioblastomas were stained with both LpMab-7 (h) and LpMab-2 (c). (d, i) Esophageal lymphatic endothelial cells were stained with LpMab-7 (i), not with LpMab-2 (d). Lung type I alveolar cells were stained with LpMab-7 (j), not with LpMab-2 (e). Arrows indicate lymphatic endothelial cells (f, g, i).

Techniques Used: Expressing, Incubation, Staining

4) Product Images from "Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN"

Article Title: Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN

Journal: Cancer Medicine

doi: 10.1002/cam4.954

Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in human cancer tissues. Tissue sections were prepared from the human cancer tissues as follows: glioblastoma ( GBM , A and E); oral squamous cell carcinoma ( OSCC ; B, D, F, and H); seminoma ( SE , C and G). Sections were incubated (antigen retrieval omitted) with 1 μ g/mL of LpMab‐21 (A–D), reacted with the Envision+ kit, color was developed using DAB , and samples were then counterstained with hematoxylin. Sections were stained with hematoxylin and eosin as well (E–H). Arrowheads: lymphatic endothelial cells. Scale bar = 100 μ m. DAB, 3‐diaminobenzidine tetrahydrochloride
Figure Legend Snippet: Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in human cancer tissues. Tissue sections were prepared from the human cancer tissues as follows: glioblastoma ( GBM , A and E); oral squamous cell carcinoma ( OSCC ; B, D, F, and H); seminoma ( SE , C and G). Sections were incubated (antigen retrieval omitted) with 1 μ g/mL of LpMab‐21 (A–D), reacted with the Envision+ kit, color was developed using DAB , and samples were then counterstained with hematoxylin. Sections were stained with hematoxylin and eosin as well (E–H). Arrowheads: lymphatic endothelial cells. Scale bar = 100 μ m. DAB, 3‐diaminobenzidine tetrahydrochloride

Techniques Used: Immunohistochemistry, Expressing, Incubation, Staining

Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in normal human tissues. Tissues harvested from the esophagus (A), breast (B), colon (C), lung (D), kidney (E), and rectum (F). After antigen retrieval procedure, sections were incubated with 1 μ g/mL of LpMab‐21, reacted with the Envision+ kit, color was developed, using DAB , and samples were then counterstained with hematoxylin. Arrowheads, lymphatic endothelial cells. Scale bar , 100 μ m. 3, DAB, 3‐diaminobenzidine tetrahydrochloride.
Figure Legend Snippet: Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in normal human tissues. Tissues harvested from the esophagus (A), breast (B), colon (C), lung (D), kidney (E), and rectum (F). After antigen retrieval procedure, sections were incubated with 1 μ g/mL of LpMab‐21, reacted with the Envision+ kit, color was developed, using DAB , and samples were then counterstained with hematoxylin. Arrowheads, lymphatic endothelial cells. Scale bar , 100 μ m. 3, DAB, 3‐diaminobenzidine tetrahydrochloride.

Techniques Used: Immunohistochemistry, Expressing, Incubation

Related Articles

Staining:

Article Title: Upregulation and intrarenal redistribution of heat shock proteins 90? and 90? by low-sodium diet in the rat
Article Snippet: .. Slides were washed and incubated with biotinylated secondary antibody, and staining was completed using 3′3-diaminobenzidine tetrahydrochloride as chromogen (DAKO Liquid DAB+, DAKO Corp., Carpinteria, CA, USA), followed by counterstaining with Meyer hematoxylin. ..

Incubation:

Article Title: Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN
Article Snippet: .. After antigen retrieval, (autoclaving using citrate buffer, pH 6.0), sections were incubated with 1 μ g/mL of LpMab‐21 for 1 h at room temperature, and immunocomplexes were treated with an Envision+ Kit (Dako) for 30 min, color was developed using 3, 3‐diaminobenzidine tetrahydrochloride (DAB, Dako) for 5 min. .. Sections were then counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

Article Title: Antidepressant effects of ginsenoside Rf on behavioral change in the glial degeneration model of depression by reversing glial loss
Article Snippet: .. After incubation with avidin-conjugated peroxidase complex (ABC kit; Vector Laboratories, CA, USA), signals were detected using 3, 3′-diaminobenzidine tetrahydrochloride (Dako, CA, USA). .. Microscopic analysis was performed using Olympus BX51 microscope (Olympus, Tokyo, Japan).

Article Title: Upregulation and intrarenal redistribution of heat shock proteins 90? and 90? by low-sodium diet in the rat
Article Snippet: .. Slides were washed and incubated with biotinylated secondary antibody, and staining was completed using 3′3-diaminobenzidine tetrahydrochloride as chromogen (DAKO Liquid DAB+, DAKO Corp., Carpinteria, CA, USA), followed by counterstaining with Meyer hematoxylin. ..

Article Title: Experimental abdominal aortic aneurysm formation is mediated by IL-17 and attenuated by mesenchymal stem cell treatment
Article Snippet: .. After incubation with an avidin-biotin complex, immunoreactivity was visualized by incubating the sections with 3, 3-diaminobenzidine tetrahydrochloride (DAKO Corp, Carpinteria, CA) to produce a brown precipitate. .. For smooth muscle α-actin (SM-αA) staining, the alkaline phosphatase-conjugated monoclonal anti-SM-αA antibody (Sigma, St Louis MO) was used.

Avidin-Biotin Assay:

Article Title: Antidepressant effects of ginsenoside Rf on behavioral change in the glial degeneration model of depression by reversing glial loss
Article Snippet: .. After incubation with avidin-conjugated peroxidase complex (ABC kit; Vector Laboratories, CA, USA), signals were detected using 3, 3′-diaminobenzidine tetrahydrochloride (Dako, CA, USA). .. Microscopic analysis was performed using Olympus BX51 microscope (Olympus, Tokyo, Japan).

Article Title: Experimental abdominal aortic aneurysm formation is mediated by IL-17 and attenuated by mesenchymal stem cell treatment
Article Snippet: .. After incubation with an avidin-biotin complex, immunoreactivity was visualized by incubating the sections with 3, 3-diaminobenzidine tetrahydrochloride (DAKO Corp, Carpinteria, CA) to produce a brown precipitate. .. For smooth muscle α-actin (SM-αA) staining, the alkaline phosphatase-conjugated monoclonal anti-SM-αA antibody (Sigma, St Louis MO) was used.

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    Agilent technologies 3 diaminobenzidine tetrahydrochloride
    Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, <t>3-diaminobenzidine</t> <t>tetrahydrochloride,</t> and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.
    3 Diaminobenzidine Tetrahydrochloride, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Agilent technologies
    Average 92 stars, based on 525 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

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    Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

    Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

    Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

    doi: 10.1089/mab.2017.0008

    Figure Lengend Snippet: Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

    Article Snippet: Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 5 minutes, and then the sections were counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

    Techniques: Immunohistochemistry, Incubation, Staining

    Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

    Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

    Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

    doi: 10.1089/mab.2017.0008

    Figure Lengend Snippet: Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

    Article Snippet: Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 5 minutes, and then the sections were counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

    Techniques: Immunohistochemistry, Incubation, Staining

    Immunohistochemical analysis of anti-PODXL antibodies in oral squamous cell carcinomas Tissue sections (obtained from Case 174) were incubated with 5 μg/mL PcMab-47 (A, F) , 0.5 μg/mL of 47-mG 2a (B, G) , 0.5 μg/mL 47-mG 2a -f (C, H) , or control (blocking buffer; D, I ) for 1 h at room temperature followed by treatment with an Envision+ kit for 30 min. Color was developed using 3,3-diaminobenzidine tetrahydrochloride for 2 min, and sections were then counterstained with hematoxylin. (E, J) Hematoxylin eosin staining. Arrows: endothelial cells. Scale bar = 100 μm.

    Journal: Oncotarget

    Article Title: Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma

    doi: 10.18632/oncotarget.25132

    Figure Lengend Snippet: Immunohistochemical analysis of anti-PODXL antibodies in oral squamous cell carcinomas Tissue sections (obtained from Case 174) were incubated with 5 μg/mL PcMab-47 (A, F) , 0.5 μg/mL of 47-mG 2a (B, G) , 0.5 μg/mL 47-mG 2a -f (C, H) , or control (blocking buffer; D, I ) for 1 h at room temperature followed by treatment with an Envision+ kit for 30 min. Color was developed using 3,3-diaminobenzidine tetrahydrochloride for 2 min, and sections were then counterstained with hematoxylin. (E, J) Hematoxylin eosin staining. Arrows: endothelial cells. Scale bar = 100 μm.

    Article Snippet: Sections were then incubated with 0.5–5 μg/mL primary mAbs for 1 h at room temperature and then treated using an Envision+ kit (Agilent Technologies) for 30 min. Color was developed using 3,3-diaminobenzidine tetrahydrochloride (Agilent Technologies) for 2 min, and sections were then counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

    Techniques: Immunohistochemistry, Incubation, Blocking Assay, Staining

    Immunohistochemical analysis of anti-PODXL antibodies in normal tongue Tissue sections of normal tongue were incubated with 5 μg/mL PcMab-47 (A, D) and 0.5 μg/mL of 47-mG 2a (B, E) for 1 h at room temperature followed by treatment with an Envision+ kit for 30 min. Color was developed using 3,3-diaminobenzidine tetrahydrochloride for 2 min. Sections were then counterstained with hematoxylin. (C, F) Hematoxylin eosin staining. Arrows: endothelial cells. Scale bar = 100 μm.

    Journal: Oncotarget

    Article Title: Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma

    doi: 10.18632/oncotarget.25132

    Figure Lengend Snippet: Immunohistochemical analysis of anti-PODXL antibodies in normal tongue Tissue sections of normal tongue were incubated with 5 μg/mL PcMab-47 (A, D) and 0.5 μg/mL of 47-mG 2a (B, E) for 1 h at room temperature followed by treatment with an Envision+ kit for 30 min. Color was developed using 3,3-diaminobenzidine tetrahydrochloride for 2 min. Sections were then counterstained with hematoxylin. (C, F) Hematoxylin eosin staining. Arrows: endothelial cells. Scale bar = 100 μm.

    Article Snippet: Sections were then incubated with 0.5–5 μg/mL primary mAbs for 1 h at room temperature and then treated using an Envision+ kit (Agilent Technologies) for 30 min. Color was developed using 3,3-diaminobenzidine tetrahydrochloride (Agilent Technologies) for 2 min, and sections were then counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

    Techniques: Immunohistochemistry, Incubation, Staining

    Immunohistochemical analysis by EMab-51 against oral cancers. Sections were incubated with 5 μg/mL of primary EMab-51 for 1 hour at room temperature followed by treatment with EnVision+ Kit for 30 minutes. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB) for 2 minutes, and sections were then counterstained with hematoxylin. (A–D) Case No. 15; (E–H) case No. 23; (A, B, E, F) immunostaining by EMab-51; (C, D, G, H) hematoxylin and eosin staining; scale bar =100 μm.

    Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

    Article Title: Development of EMab-51, a Sensitive and Specific Anti-Epidermal Growth Factor Receptor Monoclonal Antibody in Flow Cytometry, Western Blot, and Immunohistochemistry

    doi: 10.1089/mab.2017.0028

    Figure Lengend Snippet: Immunohistochemical analysis by EMab-51 against oral cancers. Sections were incubated with 5 μg/mL of primary EMab-51 for 1 hour at room temperature followed by treatment with EnVision+ Kit for 30 minutes. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB) for 2 minutes, and sections were then counterstained with hematoxylin. (A–D) Case No. 15; (E–H) case No. 23; (A, B, E, F) immunostaining by EMab-51; (C, D, G, H) hematoxylin and eosin staining; scale bar =100 μm.

    Article Snippet: Color was developed using 3,3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 2 minutes, and sections were then counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

    Techniques: Immunohistochemistry, Incubation, Immunostaining, Staining

    Immunohistochemical analysis by CMab-43 against colon cancer tissues. (A, B) Sections were incubated with 1 μg/mL of CMab-43 for 1 hour at room temperature followed by treatment with Envision+ kit for 30 minutes. Color was developed using 3,3-diaminobenzidine tetrahydrochloride for 2 minutes, and sections were then counterstained with hematoxylin. (C, D) Hematoxylin eosin staining; scale bar = 100 μm.

    Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

    Article Title: Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry

    doi: 10.1089/mab.2017.0031

    Figure Lengend Snippet: Immunohistochemical analysis by CMab-43 against colon cancer tissues. (A, B) Sections were incubated with 1 μg/mL of CMab-43 for 1 hour at room temperature followed by treatment with Envision+ kit for 30 minutes. Color was developed using 3,3-diaminobenzidine tetrahydrochloride for 2 minutes, and sections were then counterstained with hematoxylin. (C, D) Hematoxylin eosin staining; scale bar = 100 μm.

    Article Snippet: Color was developed using 3,3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 2 minutes, and sections were then counterstained with hematoxylin (Wako Pure Chemical Industries, Ltd.).

    Techniques: Immunohistochemistry, Incubation, Staining