3 diaminobenzidine tetrahydrochloride (Agilent technologies)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Agilent technologies 3 diaminobenzidine tetrahydrochloride
Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, <t>3-diaminobenzidine</t> <t>tetrahydrochloride,</t> and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, <t>3-diaminobenzidine</t> <t>tetrahydrochloride,</t> and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

3 Diaminobenzidine Tetrahydrochloride, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Agilent technologies
Average 97 stars, based on 61 article reviews
Price from $9.99 to $1999.99

3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2021-02

97/100 stars

Images

1) Product Images from "PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry"

Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

doi: 10.1089/mab.2017.0008

Figure Legend Snippet: Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

Techniques Used: Immunohistochemistry, Incubation, Staining

Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

Figure Legend Snippet: Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

Techniques Used: Immunohistochemistry, Incubation, Staining

2) Product Images from "KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association"

Article Title: KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association

Journal: Molecular Vision

doi:

KIF14 and E2F3 expression in tumor and control retina. A and D show no immunoreactivity of KIF14 and E2F3 in non-neoplastic retina. B and C show positive nucleocytoplasm (arrow heads) and nuclear (arrows) expression of KIF14 in tumor cells. E and F show positive nuclear (arrows) expression of E2F3 in tumor cells. All are 3,3′-diaminobenzidine staining with hematoxylin counterstain. The pictures were taken under 40X magnification.

Figure Legend Snippet: KIF14 and E2F3 expression in tumor and control retina. A and D show no immunoreactivity of KIF14 and E2F3 in non-neoplastic retina. B and C show positive nucleocytoplasm (arrow heads) and nuclear (arrows) expression of KIF14 in tumor cells. E and F show positive nuclear (arrows) expression of E2F3 in tumor cells. All are 3,3′-diaminobenzidine staining with hematoxylin counterstain. The pictures were taken under 40X magnification.

Techniques Used: Expressing, Staining

3) Product Images from "A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin"

Article Title: A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin

Journal: Scientific Reports

doi: 10.1038/srep05924

Podoplanin protein expression was determined immunohist ochemically in paraffin-embedded tumor specimens. Histologic sections 4 µm thick were deparaffinized in xylene and rehydrated, then autoclaved in citrate buffer (pH 6.0) for 20 min. Sections were incubated with 5 μg/ml of LpMab-2 (a-e) or LpMab-7 (f–j) overnight at 4°C with subsequent treatment using an LSAB kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB) for 10 min and was counterstained with hematoxylin. (a, f) Esophageal squamous cell carcinomas include both cancer cells (upper) and lymphatic endothelial cells (lower). Cancer cells were stained with both LpMab-7 (f) and LpMab-2 (a), whereas lymphatic endothelial cells were stained with LpMab-7 (f), not with LpMab-2 (a). (b, g) Seminoma includes both cancer cells (left) and lymphatic endothelial cells (right, upper). Cancer cells were stained with both LpMab-7 (g) and LpMab-2 (b), whereas lymphatic endothelial cells were stained with LpMab-7 (g), not with LpMab-2 (b). (c, h) Glioblastomas were stained with both LpMab-7 (h) and LpMab-2 (c). (d, i) Esophageal lymphatic endothelial cells were stained with LpMab-7 (i), not with LpMab-2 (d). Lung type I alveolar cells were stained with LpMab-7 (j), not with LpMab-2 (e). Arrows indicate lymphatic endothelial cells (f, g, i).

Figure Legend Snippet: Podoplanin protein expression was determined immunohist ochemically in paraffin-embedded tumor specimens. Histologic sections 4 µm thick were deparaffinized in xylene and rehydrated, then autoclaved in citrate buffer (pH 6.0) for 20 min. Sections were incubated with 5 μg/ml of LpMab-2 (a-e) or LpMab-7 (f–j) overnight at 4°C with subsequent treatment using an LSAB kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB) for 10 min and was counterstained with hematoxylin. (a, f) Esophageal squamous cell carcinomas include both cancer cells (upper) and lymphatic endothelial cells (lower). Cancer cells were stained with both LpMab-7 (f) and LpMab-2 (a), whereas lymphatic endothelial cells were stained with LpMab-7 (f), not with LpMab-2 (a). (b, g) Seminoma includes both cancer cells (left) and lymphatic endothelial cells (right, upper). Cancer cells were stained with both LpMab-7 (g) and LpMab-2 (b), whereas lymphatic endothelial cells were stained with LpMab-7 (g), not with LpMab-2 (b). (c, h) Glioblastomas were stained with both LpMab-7 (h) and LpMab-2 (c). (d, i) Esophageal lymphatic endothelial cells were stained with LpMab-7 (i), not with LpMab-2 (d). Lung type I alveolar cells were stained with LpMab-7 (j), not with LpMab-2 (e). Arrows indicate lymphatic endothelial cells (f, g, i).

Techniques Used: Expressing, Incubation, Staining

4) Product Images from "Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN"

Article Title: Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN

Journal: Cancer Medicine

doi: 10.1002/cam4.954

Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in human cancer tissues. Tissue sections were prepared from the human cancer tissues as follows: glioblastoma ( GBM , A and E); oral squamous cell carcinoma ( OSCC ; B, D, F, and H); seminoma ( SE , C and G). Sections were incubated (antigen retrieval omitted) with 1 μ g/mL of LpMab‐21 (A–D), reacted with the Envision+ kit, color was developed using DAB , and samples were then counterstained with hematoxylin. Sections were stained with hematoxylin and eosin as well (E–H). Arrowheads: lymphatic endothelial cells. Scale bar = 100 μ m. DAB, 3‐diaminobenzidine tetrahydrochloride

Figure Legend Snippet: Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in human cancer tissues. Tissue sections were prepared from the human cancer tissues as follows: glioblastoma ( GBM , A and E); oral squamous cell carcinoma ( OSCC ; B, D, F, and H); seminoma ( SE , C and G). Sections were incubated (antigen retrieval omitted) with 1 μ g/mL of LpMab‐21 (A–D), reacted with the Envision+ kit, color was developed using DAB , and samples were then counterstained with hematoxylin. Sections were stained with hematoxylin and eosin as well (E–H). Arrowheads: lymphatic endothelial cells. Scale bar = 100 μ m. DAB, 3‐diaminobenzidine tetrahydrochloride

Techniques Used: Immunohistochemistry, Expressing, Incubation, Staining

Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in normal human tissues. Tissues harvested from the esophagus (A), breast (B), colon (C), lung (D), kidney (E), and rectum (F). After antigen retrieval procedure, sections were incubated with 1 μ g/mL of LpMab‐21, reacted with the Envision+ kit, color was developed, using DAB , and samples were then counterstained with hematoxylin. Arrowheads, lymphatic endothelial cells. Scale bar , 100 μ m. 3, DAB, 3‐diaminobenzidine tetrahydrochloride.

Figure Legend Snippet: Immunohistochemical analysis using LpMab‐21 to detect PDPN expression in normal human tissues. Tissues harvested from the esophagus (A), breast (B), colon (C), lung (D), kidney (E), and rectum (F). After antigen retrieval procedure, sections were incubated with 1 μ g/mL of LpMab‐21, reacted with the Envision+ kit, color was developed, using DAB , and samples were then counterstained with hematoxylin. Arrowheads, lymphatic endothelial cells. Scale bar , 100 μ m. 3, DAB, 3‐diaminobenzidine tetrahydrochloride.

Techniques Used: Immunohistochemistry, Expressing, Incubation

Staining:

Article Title: Human adipose-derived mesenchymal stem cells accelerate decellularized neobladder regeneration
Article Snippet: .. Deparaffinized and hydrated sagittal slices of 4 μm tissue sections were stained with hematoxylin–eosin (Coverstainer, Dako) or Masson Trichrome (MT) (Artisanlinkl pro, Dako). .. For immunohistochemistry (IHQ), the paraffin-embedded sections were first deparaffinized, processed for antigen retrieval by incubation in citric acid-based un-masking solution (Vector Laboratories), permeabilized with a PBS solution containing 0.1% Triton X-100, and blocked with 5% goat serum in PBS for 1 h. The following primary antibodies were diluted in blocking solution and incubated for 60 min at room temperature at 1:100 dilutions: monoclonal mouse anti-P63 (IR662), anti-Cytokeratin 7 (IR619), anti-smooth muscle actin (SMA) (IR611), anti-Desmin (IR606), anti-Vimentin (IR630), anti-S100β (IR504), anti-CD31 (IR610) and anti-Ki67 (IR626) from Dako or anti-human mitochondria (MAB1273) from Chemicon.

Article Title: PARP Theranostic Auger Emitters Are Cytotoxic in BRCA Mutant Ovarian Cancer and Viable Tumors from Ovarian Cancer Patients Enable Ex-Vivo Screening of Tumor Response
Article Snippet: .. Blocks were cut into 5 μm sections and stained using the DAKO CoverStainer for H & E (Agilent, Santa Clara, CA, USA). .. Immunohistochemistry was performed using the Leica Bond-IIITM with the Bond Polymer Refine Detection System.

Article Title: High excision repair cross-complementation group 1 expression is associated with favorable prognostic factors in breast cancer
Article Snippet: .. Tissue section (3-µm-thick) were stained with hematoxylin (at room temperature for 90 sec) and eosin (at room temperature for 40 sec) using Dako Coverstainer fully automated system (Dako, Agilent Technologies, Inc., Santa Clara, CA, USA) and slides from all patients were reviewed by two pathologists in a blind manner with an Olympus BX51 microscope, and the histological data such as T and N stage, and lymphatic invasion, were confirmed again. ..

Article Title: Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice
Article Snippet: .. Sections were cut at 3-5 µm thickness, dewaxed and stained with Hematoxylin and Eosin (H & E) and PAS (Sigma-Aldrich) using a CoverStainer (Dako-Agilent) following the manufacturer’s instructions. .. For immunohistochemistry (IHC), epitope retrieval was performed using citrate pH 6 buffer in an autoclave at 121°C for 12 min. Washings were performed using the Wash Solution AR (AR10211-2, Dako, Agilent).

Article Title: Establishment of patient‐derived xenograft model of peritoneal mucinous carcinomatosis with signet ring cells and in vivo study on the efficacy and toxicity of intraperitoneal injection of 5‐fluorouracil, et al. Establishment of patient‐derived xenograft model of peritoneal mucinous carcinomatosis with signet ring cells and in vivo study on the efficacy and toxicity of intraperitoneal injection of 5‐fluorouracil
Article Snippet: .. H & E staining (Dako Hematoxylin, Dako Eosin and Dako Bluing Buffer, catalog number CS701) was performed on Dako CoverStainer for H & E (Agilent Technologies, Inc). .. Sections for IHC analyses were baked for 1 hour, deparaffinized in dimethylbenzene for two times, rehydrated through a graded series of alcohol to distilled water, following antigen retrieval by heat treatment for 4 minutes in citrate buffer (pH 8.4) and quenching of endogenous peroxidase activity for 10 minutes using 3% hydrogen peroxide.

Article Title: A Phase II Study of Glembatumumab Vedotin for Metastatic Uveal Melanoma
Article Snippet: .. Upon the completion of the staining procedure, slides were counterstained with hematoxylin (Dako) for 2 min followed by a rinse in distilled water, a rinse with a wash buffer for 5 min, and then another rinse in distilled water. ..

Article Title: Advanced co-culture 3D breast cancer model for investigation of fibrosis induced by external stimuli: optimization study
Article Snippet: .. The slides were left at room temperature until the complete drying before being stained with a solution of HES (Dako CoverStainer automate, Dako, France). .. Immunohistochemical analysesThe immunohistochemical characterization of fixed cryosections of mono- or co-cultured spheroids was made using an automated system (Benchmark Ultra automaton, Ventana) for proliferative Ki67 marker (1:50 dilution, Monoclonal Mouse Anti-Human Ki67-Antigen, Clone MIN-1, Dako, France).

3 diaminobenzidine tetrahydrochloride (Agilent technologies)

Structured Review

Related Products / Commonly Used Together

Images

1) Product Images from "PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry"

2) Product Images from "KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association"

3) Product Images from "A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin"

4) Product Images from "Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR/Cas9 and TALEN"

Related Articles

Staining:

Size-exclusion Chromatography:

Microscopy:

Similar Products

Image Search Results