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Agilent technologies 3 diaminobenzidine tetrahydrochloride dab
Intestinal localization of SR-BI Sections of small intestine from wild type and transgenic mice were incubated with anti-SR-BI antibodies followed by incubation with biotinylated secondary antibodies and with streptavidin-peroxidase complex. Peroxidase activity was revealed using <t>3′</t> diaminobenzidine <t>tetrahydrochloride</t> (DAB). A,D,G: control was obtained with an incubation of wild type sections in only the secondary antibodies; B,E,H: wild type mice; C,F,I: SR-BI transgenic mice. P, Proximal; M, Medium; D, Colon. V, Villosity; C, Crypt.
3 Diaminobenzidine Tetrahydrochloride Dab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride dab/product/Agilent technologies
Average 97 stars, based on 15 article reviews
Price from $9.99 to $1999.99
3 diaminobenzidine tetrahydrochloride dab - by Bioz Stars, 2021-02
97/100 stars

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1) Product Images from "Accelerated lipid absorption in mice overexpressing intestinal SR-BI"

Article Title: Accelerated lipid absorption in mice overexpressing intestinal SR-BI

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M508868200

Intestinal localization of SR-BI Sections of small intestine from wild type and transgenic mice were incubated with anti-SR-BI antibodies followed by incubation with biotinylated secondary antibodies and with streptavidin-peroxidase complex. Peroxidase activity was revealed using 3′ diaminobenzidine tetrahydrochloride (DAB). A,D,G: control was obtained with an incubation of wild type sections in only the secondary antibodies; B,E,H: wild type mice; C,F,I: SR-BI transgenic mice. P, Proximal; M, Medium; D, Colon. V, Villosity; C, Crypt.
Figure Legend Snippet: Intestinal localization of SR-BI Sections of small intestine from wild type and transgenic mice were incubated with anti-SR-BI antibodies followed by incubation with biotinylated secondary antibodies and with streptavidin-peroxidase complex. Peroxidase activity was revealed using 3′ diaminobenzidine tetrahydrochloride (DAB). A,D,G: control was obtained with an incubation of wild type sections in only the secondary antibodies; B,E,H: wild type mice; C,F,I: SR-BI transgenic mice. P, Proximal; M, Medium; D, Colon. V, Villosity; C, Crypt.

Techniques Used: Transgenic Assay, Mouse Assay, Incubation, Activity Assay

Related Articles

TUNEL Assay:

Article Title: Phase II Prospective Randomized Trial of Weight Loss Prior to Radical Prostatectomy (PCAN-17-0128)
Article Snippet: .. Slides were stained for Ki67 (monoclonal mouse anti-human Ki67 antigen [Dako Omnis/Agilent, Santa Clara, CA]) and TUNEL (ApopTag® Plus Peroxidase In Situ Apoptosis Kit [Millipore, Temecula, CA]) ( ) to assess proliferation and apoptosis, respectively. ..

Immunohistochemistry:

Article Title: Automated sequential chromogenic IHC double staining with two HRP substrates
Article Snippet: .. Automated staining was performed on either a Dako Omnis system using the “IHC Double Stain Template” or an Autostainer Link 48 system (See ). .. On the Dako Omnis system the “IHC Double Stain Template” was used.

Article Title: A Case of Adenocarcinoma of Uterus Masculinus in a Pomeranian Dog
Article Snippet: .. On immunohistochemistry, neoplastic cells were positive for cytokeratin-7 (Dako Omnis, clone OV-TL 12/30; ) and vimentin (Dako Omnis, clone V9; ); moreover, smooth muscle cells and occasional neoplastic cells showed nuclear immunoreactivity for estrogen receptor α (Dako Omnis, clone EP1; ). ..

Article Title: Expression of LIGHT/TNFSF14 and Its Receptors, HVEM and LTβR, Correlates with the Severity of Fibrosis in Lacrimal Sacs from Patients with Lacrimal Duct Obstruction
Article Snippet: .. Identification of subsets of mononuclear cells was done by immunohistochemistry using specific antibodies: anti-CD68 (monoclonal mouse anti-human CD68/FITC clone KP1, Dako-Agilent) for macrophages, anti-CD3 antibody (mouse monoclonal anti-CD3 concentrate clone F7.2.38 Dako Omnis, Agilent) for T cells and anti-CD20 antibodies (monoclonal mouse clone L26, Dako Omnis, Agilent) for B cells. .. The staining was done on an Omnis autostainer using protocols, preceded by antigen retrieval in PTLink (pH = 9.0).

In Situ:

Article Title: Phase II Prospective Randomized Trial of Weight Loss Prior to Radical Prostatectomy (PCAN-17-0128)
Article Snippet: .. Slides were stained for Ki67 (monoclonal mouse anti-human Ki67 antigen [Dako Omnis/Agilent, Santa Clara, CA]) and TUNEL (ApopTag® Plus Peroxidase In Situ Apoptosis Kit [Millipore, Temecula, CA]) ( ) to assess proliferation and apoptosis, respectively. ..

Blocking Assay:

Article Title: Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis
Article Snippet: .. Briefly, after quenching endogenous peroxidase activity and blocking nonspecific binding, sections were reacted with mouse anti-human sEH (A-5) monoclonal antibody (1 : 10 dilution; catalog no. SC-166961; Santa Cruz Biotechnology, Inc. Dallas, TX, USA), anti-human CK7 monoclonal antibody (1 : 1000 dilution, clone OV-TL 12/30, Dako Omnis, Agilent, Santa Clara, CA, USA), or anti-human CD68 monoclonal antibody (1 : 1000 dilution, clone KP1, Dako Omnis) at 4°C overnight. .. Further processing for colorimetric detection was according to the instructions for the VECTASTAIN Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) using diaminobenzidine as the peroxidase substrate.

Incubation:

Article Title: The Pro-Oxidant, Apoptotic and Anti-Angiogenic Effects of Selenium Supplementation on Colorectal Tumors Induced by 1,2-Dimethylhydrazine in BALB/C Mice
Article Snippet: .. After that, the sections were incubated separately overnight with the following primary antibodies: CDX-2 monoclonal antibody (M3636, Dako Omnis, UK), VEGF polyclonal antibody (RB-222-R7, Thermo Fisher Scientific, USA), caspase-3 polyclonal antibody (PA1-29157, Thermo Fisher Scientific, USA). .. The slides were washed thoroughly and then incubated with secondary antibody: (HRP Envision kit, DAKO, Agilent, USA).

Article Title: Comparative pathogenesis of Ebola virus and Reston virus infection in humanized mice
Article Snippet: .. Sections were blocked (PBS/10% FCS) and incubated with primary antibodies for rat anti–mouse CD3 (T cells; OKT; 317301, BioLegend), anti-CD20 (B cells; Dako Omnis clone L26; GA604), anti–cleaved caspase-3 (apoptosis marker; ab2302, Abcam), anti-CD14 (monocytes; ab183322, Abcam), or anti–Ebola-NP (clone KZ51 IgG1 mouse; Absolute Antibody). .. Bound primary antibodies were detected with anti-mouse, anti-rabbit or anti-rat N -Histofine Simple Stain MAX PO immune-enzyme polymer (Nichirei Biosciences Inc.) and stained with DAB substrate using the ultraView Universal DAB Detection Kit (Ventana).

Activity Assay:

Article Title: Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis
Article Snippet: .. Briefly, after quenching endogenous peroxidase activity and blocking nonspecific binding, sections were reacted with mouse anti-human sEH (A-5) monoclonal antibody (1 : 10 dilution; catalog no. SC-166961; Santa Cruz Biotechnology, Inc. Dallas, TX, USA), anti-human CK7 monoclonal antibody (1 : 1000 dilution, clone OV-TL 12/30, Dako Omnis, Agilent, Santa Clara, CA, USA), or anti-human CD68 monoclonal antibody (1 : 1000 dilution, clone KP1, Dako Omnis) at 4°C overnight. .. Further processing for colorimetric detection was according to the instructions for the VECTASTAIN Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) using diaminobenzidine as the peroxidase substrate.

Marker:

Article Title: Comparative pathogenesis of Ebola virus and Reston virus infection in humanized mice
Article Snippet: .. Sections were blocked (PBS/10% FCS) and incubated with primary antibodies for rat anti–mouse CD3 (T cells; OKT; 317301, BioLegend), anti-CD20 (B cells; Dako Omnis clone L26; GA604), anti–cleaved caspase-3 (apoptosis marker; ab2302, Abcam), anti-CD14 (monocytes; ab183322, Abcam), or anti–Ebola-NP (clone KZ51 IgG1 mouse; Absolute Antibody). .. Bound primary antibodies were detected with anti-mouse, anti-rabbit or anti-rat N -Histofine Simple Stain MAX PO immune-enzyme polymer (Nichirei Biosciences Inc.) and stained with DAB substrate using the ultraView Universal DAB Detection Kit (Ventana).

Article Title: Alveolar macrophage dysfunction and cytokine storm in the pathogenesis of two severe COVID-19 patients
Article Snippet: .. A panel of primary antibodies were used, including the macrophage marker CD68 (Monoclonal mouse anti-human CD68, clone KP1; 1:100; Dako Omnis, Agilent); T-lymphocyte marker CD3 (Monoclonal rabbit anti-human CD3, clone SP7; ready-to-use; Dako Omnis, Agilent), CD4 (Monoclonal mouse anti-human CD4, clone 4B12; ready-to-use; Dako Omnis, Agilent) and CD8 (Monoclonal mouse anti-human CD8, clone C8/144B; ready-to-use); B-lymphocyte markers CD20 (Monoclonal mouse anti-human CD20cy, clone L26; ready-to-use); natural killer cell/T cell (NK/T cell) marker CD56 (Monoclonal mouse anti-human CD56, clone 123C3; ready-to-use), and the markers of Programmed Cell Death-1 (PD-1 Monoclonal mouse anti-human PD-1, clone UMAB199; ready-to-use) and Programmed Cell Death-Ligand 1 (PD-L1 Monoclonal mouse anti-human PD-L1, clone 22C3; ready-to-use). .. Antibodies specific for chemokine and inflammatory cytokines were also used, including interleukin 6 (polyclonal rabbit anti-IL-6 human; 1:250; abcam), interleukin 10 (IL-10, polyclonal rabbit anti-human IL-10; 1:300; abcam) and tumor necrosis factor α (TNFα, polyclonal rabbit anti-human TNFα; 1:80; abcam).

Staining:

Article Title: Automated sequential chromogenic IHC double staining with two HRP substrates
Article Snippet: .. Automated staining was performed on either a Dako Omnis system using the “IHC Double Stain Template” or an Autostainer Link 48 system (See ). .. On the Dako Omnis system the “IHC Double Stain Template” was used.

Article Title: Phase II Prospective Randomized Trial of Weight Loss Prior to Radical Prostatectomy (PCAN-17-0128)
Article Snippet: .. Slides were stained for Ki67 (monoclonal mouse anti-human Ki67 antigen [Dako Omnis/Agilent, Santa Clara, CA]) and TUNEL (ApopTag® Plus Peroxidase In Situ Apoptosis Kit [Millipore, Temecula, CA]) ( ) to assess proliferation and apoptosis, respectively. ..

Binding Assay:

Article Title: Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis
Article Snippet: .. Briefly, after quenching endogenous peroxidase activity and blocking nonspecific binding, sections were reacted with mouse anti-human sEH (A-5) monoclonal antibody (1 : 10 dilution; catalog no. SC-166961; Santa Cruz Biotechnology, Inc. Dallas, TX, USA), anti-human CK7 monoclonal antibody (1 : 1000 dilution, clone OV-TL 12/30, Dako Omnis, Agilent, Santa Clara, CA, USA), or anti-human CD68 monoclonal antibody (1 : 1000 dilution, clone KP1, Dako Omnis) at 4°C overnight. .. Further processing for colorimetric detection was according to the instructions for the VECTASTAIN Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) using diaminobenzidine as the peroxidase substrate.

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  • 92
    Agilent technologies 3 diaminobenzidine tetrahydrochloride
    Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, <t>3-diaminobenzidine</t> <t>tetrahydrochloride,</t> and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.
    3 Diaminobenzidine Tetrahydrochloride, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 diaminobenzidine tetrahydrochloride/product/Agilent technologies
    Average 92 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2021-02
    92/100 stars
      Buy from Supplier

    97
    Agilent technologies 3 3 diaminobenzidine tetrahydrochloride dab reagent
    Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, <t>3-diaminobenzidine</t> <t>tetrahydrochloride,</t> and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.
    3 3 Diaminobenzidine Tetrahydrochloride Dab Reagent, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride dab reagent/product/Agilent technologies
    Average 97 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine tetrahydrochloride dab reagent - by Bioz Stars, 2021-02
    97/100 stars
      Buy from Supplier

    92
    Agilent technologies 3 3 diaminobenzidine tetrahydrochloride dab
    Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, <t>3-diaminobenzidine</t> <t>tetrahydrochloride,</t> and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.
    3 3 Diaminobenzidine Tetrahydrochloride Dab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride dab/product/Agilent technologies
    Average 92 stars, based on 531 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine tetrahydrochloride dab - by Bioz Stars, 2021-02
    92/100 stars
      Buy from Supplier

    Image Search Results


    Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

    Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

    Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

    doi: 10.1089/mab.2017.0008

    Figure Lengend Snippet: Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

    Article Snippet: Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 5 minutes, and then the sections were counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

    Techniques: Immunohistochemistry, Incubation, Staining

    Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

    Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

    Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

    doi: 10.1089/mab.2017.0008

    Figure Lengend Snippet: Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

    Article Snippet: Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 5 minutes, and then the sections were counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

    Techniques: Immunohistochemistry, Incubation, Staining