3 diaminobenzidine dab  (Millipore)


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    • 99
    Name:
    3 3 Diaminobenzidine DAB Liquid Substrate System tetrahydrochloride
    Description:

    Catalog Number:
    d7304
    Price:
    None
    Applications:
    DAB Liquid Substrate System is a precipitating substrate system for peroxidase. It produces a brown precipitate for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures. DAB has been used as a substrate in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver. It has been used as a substrate for visualization of sections after immunostaining.
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    Structured Review

    Millipore 3 diaminobenzidine dab
    Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by <t>3,3′-diaminobenzidine</t> (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    https://www.bioz.com/result/3 diaminobenzidine dab/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    3 diaminobenzidine dab - by Bioz Stars, 2021-01
    99/100 stars

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    Images

    1) Product Images from "Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield"

    Article Title: Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

    Journal: Rice

    doi: 10.1186/s12284-020-00396-2

    Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P
    Figure Legend Snippet: Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (Os KS4, OsNAC4, OsPR1, and OsPR10b ) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    Techniques Used: Blocking Assay, Staining, Microscopy, Expressing

    Osa-miR162a enhances the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and OX162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The above photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Relative mRNA levels of the defense-related genes ( OsKS4, OsNAC4, OsPR1 , and OsPR10b ) in NPB and OX162 following inoculation of M. oryzae GZ8. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P
    Figure Legend Snippet: Osa-miR162a enhances the induction of disease-related defense responses. a Hydrogen peroxide (H 2 O 2 ) accumulation in wild type (Nipponbare, NPB) and OX162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H 2 O 2 , which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The above photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Relative mRNA levels of the defense-related genes ( OsKS4, OsNAC4, OsPR1 , and OsPR10b ) in NPB and OX162 following inoculation of M. oryzae GZ8. Rice ubiquitin ( UBQ ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences ( P

    Techniques Used: Staining, Microscopy

    Related Articles

    Staining:

    Article Title: Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)
    Article Snippet: .. Cells were stained with 3,3'-diaminobenzidine (DAB) substrate solution by incubating cells in 200 μL premixed DAB solution (mix 30 μL (one drop) of the DAB liquid chromogen solution to 2 mL of the DAB liquid buffer solution (Sigma, # D 3939)) for 10 min. DAB solution was removed and cells rinsed briefly with PBS. .. Cells were counterstained with 20% Wright Giemsa solution for 1 min. Coverslips were mounted on microscope slides using a drop of permount mounting medium.

    Article Title: An Important Role for Syndecan-1 in Herpes Simplex Virus Type-1 Induced Cell-to-Cell Fusion and Virus Spread
    Article Snippet: .. Plaques were stained using rabbit anti-HSV-1 (Dako), horseradish peroxidase-conjugated secondary antibody (Amersham), and 3, 3′-diaminobenzidine tetrahydrochloride (DAB) substrate (Sigma). .. Plaques were measured with a micrometer at the 10× objective (Zeiss Axiovert 200), and the area was calculated by measuring the outline of each plaque using Axioversion software (version 4).

    Article Title: Dopaminergic Neurodegeneration in the Mouse Is Associated with Decrease of Viscoelasticity of Substantia Nigra Tissue
    Article Snippet: .. Then, ABC solution (Vectastain Elite ABC Kit, Vector Laboratories) was applied, before the formed streptavidin-peroxidase complex was visualized by 3,3'-diaminobenzidine (DAB, Sigma-Aldrich)-nickel staining. .. Finally, stained sections were mounted on microscope slides and coverslipped.

    Incubation:

    Article Title: Hypoglycaemic activity of 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione in streptozotocin-induced diabetic mice through ameliorating metabolic function and regulating peroxisome proliferator-activated receptor γ
    Article Snippet: .. Sections were incubated with rabbit anti-mouse polyclonal PPAR-γ (1 : 100) antibody (Santa Cruz Biotechnology, Inc. Texas, USA) in a moisture chamber and then treated with 3′-diaminobenzidine (DAB) 0.01% hydrogen peroxide (Sigma, St. Louis, MO USA). .. Photographs at a magnification of 200× were analysed by applying a light microscope (Olympus, CX4 Japan).

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    • 3 3 diaminobenzidine dab  (Millipore)
    99
    Millipore 3 3 diaminobenzidine dab
    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with <t>diaminobenzidine</t> <t>(DAB)</t> for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.
    3 3 Diaminobenzidine Dab, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1755 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab/product/Millipore
    Average 99 stars, based on 1755 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab - by Bioz Stars, 2021-01
    99/100 stars
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    sigmafast 3 3 diaminobenzidine tablets  (Millipore)
    90
    Millipore sigmafast 3 3 diaminobenzidine tablets
    Immunohistochemical staining of CD8 on formalin‐fixed, paraffin‐embedded (FFPE) slides of tumors obtained from the studied cervical cancer patient cohort. A, Microscopic image (400X magnification) of CD8 staining (left) and representation of the computerized quantification of membrane <t>3,3'‐diaminobenzidine</t> (DAB)‐positive cells (red) and DAB‐negative cells (blue) in QuPath image analysis software (right). B, Quantified numbers of DAB/CD8‐positive cells per mm 2 tumor tissue for the three groups: WT/WT, WT/VAR, and VAR/VAR. C, Disease‐specific survival dividing patients into two groups: below (orange) and above (green) the median number of CD8 infiltrating cells. Statistical analysis was performed by log‐rank testing. Significance was defined as P
    Sigmafast 3 3 Diaminobenzidine Tablets, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sigmafast 3 3 diaminobenzidine tablets/product/Millipore
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    sigmafast 3 3 diaminobenzidine tablets - by Bioz Stars, 2021-01
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    Image Search Results


    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Journal: Frontiers in Neuroanatomy

    Article Title: Putaminal Mosaic Visualized by Tyrosine Hydroxylase Immunohistochemistry in the Human Neostriatum

    doi: 10.3389/fnana.2016.00034

    Figure Lengend Snippet: Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Article Snippet: IHC with 3,3′-Diaminobenzidine (DAB) in Human Brain Tissue The sections were incubated for 18 h in 3% BSA-PBS containing a rabbit polyclonal antibody against TH (1:200,000; Sato et al., ) or [Met]-enkephalin (MEnk; 1:500,000; Millipore, St. Louis, MO, USA; Goto et al., ).

    Techniques: Staining, Marker

    Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Journal: Nature Communications

    Article Title: Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation

    doi: 10.1038/s41467-017-02033-x

    Figure Lengend Snippet: Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Article Snippet: Sections were rinsed with 0.1 M PBS (pH 7.4) for two, 5 min washes and then treated with 3% hydrogen peroxide for 10 min. We then used the 3,3′-Diaminobenzidine (DAB) IHC Select kit (DAB500, Millipore), but modified the company protocol (increased the duration of blocking, secondary antibody, streptavidin horseradish peroxidase, and DAB incubations to 10 min each).

    Techniques: Immunohistochemistry, Infection, Amplification

    Immunohistochemical staining of CD8 on formalin‐fixed, paraffin‐embedded (FFPE) slides of tumors obtained from the studied cervical cancer patient cohort. A, Microscopic image (400X magnification) of CD8 staining (left) and representation of the computerized quantification of membrane 3,3'‐diaminobenzidine (DAB)‐positive cells (red) and DAB‐negative cells (blue) in QuPath image analysis software (right). B, Quantified numbers of DAB/CD8‐positive cells per mm 2 tumor tissue for the three groups: WT/WT, WT/VAR, and VAR/VAR. C, Disease‐specific survival dividing patients into two groups: below (orange) and above (green) the median number of CD8 infiltrating cells. Statistical analysis was performed by log‐rank testing. Significance was defined as P

    Journal: Cancer Science

    Article Title: Association of homozygous variants of STING1 with outcome in human cervical cancer, et al. Association of homozygous variants of STING1 with outcome in human cervical cancer

    doi: 10.1111/cas.14680

    Figure Lengend Snippet: Immunohistochemical staining of CD8 on formalin‐fixed, paraffin‐embedded (FFPE) slides of tumors obtained from the studied cervical cancer patient cohort. A, Microscopic image (400X magnification) of CD8 staining (left) and representation of the computerized quantification of membrane 3,3'‐diaminobenzidine (DAB)‐positive cells (red) and DAB‐negative cells (blue) in QuPath image analysis software (right). B, Quantified numbers of DAB/CD8‐positive cells per mm 2 tumor tissue for the three groups: WT/WT, WT/VAR, and VAR/VAR. C, Disease‐specific survival dividing patients into two groups: below (orange) and above (green) the median number of CD8 infiltrating cells. Statistical analysis was performed by log‐rank testing. Significance was defined as P

    Article Snippet: Signal was visualized with 3,3'‐diaminobenzidine (DAB) solution containing hydrogen peroxide, and the slides were counterstained with hematoxylin.

    Techniques: Immunohistochemistry, Staining, Formalin-fixed Paraffin-Embedded, Software