Structured Review

Agilent technologies 3 3 diaminobenzidine dab
Neuropathological investigation of the SCN-related neurons in the hypothalamus of C9orf72 and non C9orf72 cases. VIP-ir neurons in the hypothalamus at the level of the SON and PVN were referred to as SCN-related neurons (case nonC9–16) ( a ). The inset shows VIP-immunostaining in neurons and fibers ( a ). Poly(GA) pathology (visualized by Fast red) was observed in few VIP-ir SCN-related neurons (visualized in brown with <t>DAB)</t> in half of the C9orf72 cases (case C9–3) ( b ). Inset b1 shows a magnification of a SCN-related neuron with poly(GA) pathology; inset b2 shows a magnification of a VIP-negative neuron with a poly(GA) inclusion. Poly(GA) inclusions (visualized by Fast red) were observed in the area of the SCN-related neurons and fibers (encircled) visualized in brown by DAB ( c ), however, more poly(GA) inclusions are observed in the area surrounding the SCN-related neurons and fibers (case C9–1) ( c ). SCN-related neurons were usually spared from pTDP-43 pathology. In some cases, pTDP-43 pathology (black arrows) (visualized by Fast red) was observed in VIP-negative neurons located in between the SCN-related neurons (arrowhead) and fibers (case nonC9–1) ( d ). The inset in d shows a magnification of a VIP-negative neuron with pTDP-43 pathology. Scale bars represent 1000 μm in a , 100 μm in b , 200 μm in c , 50 μm in d and inset of a , and 5 μm in insets of b and d . VIP-ir stands for vasoactive intestinal peptide-immunoreactive; SCN, suprachiasmatic nucleus; DAB, <t>3,3′-diaminobenzidine</t>
3 3 Diaminobenzidine Dab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Circadian sleep/wake-associated cells show dipeptide repeat protein aggregates in C9orf72-related ALS and FTLD cases"

Article Title: Circadian sleep/wake-associated cells show dipeptide repeat protein aggregates in C9orf72-related ALS and FTLD cases

Journal: Acta Neuropathologica Communications

doi: 10.1186/s40478-019-0845-9

Neuropathological investigation of the SCN-related neurons in the hypothalamus of C9orf72 and non C9orf72 cases. VIP-ir neurons in the hypothalamus at the level of the SON and PVN were referred to as SCN-related neurons (case nonC9–16) ( a ). The inset shows VIP-immunostaining in neurons and fibers ( a ). Poly(GA) pathology (visualized by Fast red) was observed in few VIP-ir SCN-related neurons (visualized in brown with DAB) in half of the C9orf72 cases (case C9–3) ( b ). Inset b1 shows a magnification of a SCN-related neuron with poly(GA) pathology; inset b2 shows a magnification of a VIP-negative neuron with a poly(GA) inclusion. Poly(GA) inclusions (visualized by Fast red) were observed in the area of the SCN-related neurons and fibers (encircled) visualized in brown by DAB ( c ), however, more poly(GA) inclusions are observed in the area surrounding the SCN-related neurons and fibers (case C9–1) ( c ). SCN-related neurons were usually spared from pTDP-43 pathology. In some cases, pTDP-43 pathology (black arrows) (visualized by Fast red) was observed in VIP-negative neurons located in between the SCN-related neurons (arrowhead) and fibers (case nonC9–1) ( d ). The inset in d shows a magnification of a VIP-negative neuron with pTDP-43 pathology. Scale bars represent 1000 μm in a , 100 μm in b , 200 μm in c , 50 μm in d and inset of a , and 5 μm in insets of b and d . VIP-ir stands for vasoactive intestinal peptide-immunoreactive; SCN, suprachiasmatic nucleus; DAB, 3,3′-diaminobenzidine
Figure Legend Snippet: Neuropathological investigation of the SCN-related neurons in the hypothalamus of C9orf72 and non C9orf72 cases. VIP-ir neurons in the hypothalamus at the level of the SON and PVN were referred to as SCN-related neurons (case nonC9–16) ( a ). The inset shows VIP-immunostaining in neurons and fibers ( a ). Poly(GA) pathology (visualized by Fast red) was observed in few VIP-ir SCN-related neurons (visualized in brown with DAB) in half of the C9orf72 cases (case C9–3) ( b ). Inset b1 shows a magnification of a SCN-related neuron with poly(GA) pathology; inset b2 shows a magnification of a VIP-negative neuron with a poly(GA) inclusion. Poly(GA) inclusions (visualized by Fast red) were observed in the area of the SCN-related neurons and fibers (encircled) visualized in brown by DAB ( c ), however, more poly(GA) inclusions are observed in the area surrounding the SCN-related neurons and fibers (case C9–1) ( c ). SCN-related neurons were usually spared from pTDP-43 pathology. In some cases, pTDP-43 pathology (black arrows) (visualized by Fast red) was observed in VIP-negative neurons located in between the SCN-related neurons (arrowhead) and fibers (case nonC9–1) ( d ). The inset in d shows a magnification of a VIP-negative neuron with pTDP-43 pathology. Scale bars represent 1000 μm in a , 100 μm in b , 200 μm in c , 50 μm in d and inset of a , and 5 μm in insets of b and d . VIP-ir stands for vasoactive intestinal peptide-immunoreactive; SCN, suprachiasmatic nucleus; DAB, 3,3′-diaminobenzidine

Techniques Used: Immunostaining

DPR and pTDP-43 pathology in the pineal gland of C9orf72 and non C9orf72 cases. Poly(GA) ( a ) and poly(GP) ( b ) inclusions were observed in the pineal gland of all C9orf72 cases. To a lesser extent, poly(GR) ( c ) and poly(PR) ( d ) inclusions were present in the pineal gland of C9orf72 cases. Panels a-d show immunohistochemical stainings of case C9–7. The insets show a magnification of the respective DPR inclusions. The chromogen used for visualization of poly(GP), poly(GR) and poly(PR) inclusions is a Fast Red-type chromogen, therefore, a red background color was obtained. Poly(GA) inclusions (visualized by DAB) are present in the melatonin-producing pinealocytes expressing synaptophysin (visualized by Fast red) shown here in case C9–1 ( e ). The inset of e shows a magnification of a synaptophysin-positive neuron with a poly(GA) inclusion. The pineal gland is devoid of pTDP-43 pathology (visualized by Fast red) ( f ). Endogenous brown colored material was observed in the tissue ( b-d, f ). Scale bar represents 50 μm, the scale bar of the insets represents 5 μm. DAB stands for 3,3′-diaminobenzidine
Figure Legend Snippet: DPR and pTDP-43 pathology in the pineal gland of C9orf72 and non C9orf72 cases. Poly(GA) ( a ) and poly(GP) ( b ) inclusions were observed in the pineal gland of all C9orf72 cases. To a lesser extent, poly(GR) ( c ) and poly(PR) ( d ) inclusions were present in the pineal gland of C9orf72 cases. Panels a-d show immunohistochemical stainings of case C9–7. The insets show a magnification of the respective DPR inclusions. The chromogen used for visualization of poly(GP), poly(GR) and poly(PR) inclusions is a Fast Red-type chromogen, therefore, a red background color was obtained. Poly(GA) inclusions (visualized by DAB) are present in the melatonin-producing pinealocytes expressing synaptophysin (visualized by Fast red) shown here in case C9–1 ( e ). The inset of e shows a magnification of a synaptophysin-positive neuron with a poly(GA) inclusion. The pineal gland is devoid of pTDP-43 pathology (visualized by Fast red) ( f ). Endogenous brown colored material was observed in the tissue ( b-d, f ). Scale bar represents 50 μm, the scale bar of the insets represents 5 μm. DAB stands for 3,3′-diaminobenzidine

Techniques Used: Immunohistochemistry, Expressing

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Article Snippet: After washing, a peroxidase-conjugated anti-mouse antibody (1∶50, Jackson ImmunoResearch Laboratories) was added, and the reaction was developed by 0.05% 3,3′- diaminobenzidine (DAB) (DAKO) in PBS containing 0.15% H2 O2 .

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Article Snippet: For DAB stainings, the secondary antibody (anti-mouse HRP, P0447 Dako; 1:200) was detected by 3,3-diaminobenzidine (DAB) as the chromogen, and DAB sections were imaged using a Nanozoomer scanner.

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Article Snippet: The bound antibodies were eventually visualized using 3,3′-diaminobenzidine (DAB) as a chromogen (Dako), and the slides were counterstained with hematoxylin.

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Article Snippet: The Avidin-Biotin Complex technique (Vectastain Elite kit; ABC-HRP Kit; Vector Laboratories, Burlingame, CA, USA) coupled to 3,3′-Diaminobenzidine (DAB) (Dako, Carpinteria, CA) developing was used for immunohistochemistry.

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Article Snippet: Reactions were visualized by using 3,3-Diaminobenzidine (DAB+) (DAKO, K3468) and counterstaining with hematoxylin.

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Article Snippet: Immunoreactivity was detected with 3,3-diaminobenzidine (DAB) using Envision+ System-HRP (Dako Cytomation) according to the manufacturer’s instruction.

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Article Snippet: After 3 additional washes in phosphate-buffered saline, the immune reaction was visualized with 3,3′-diaminobenzidine (DAB) (Dako, Copenhagen, Denmark).

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Article Snippet: Staining with 3,3′-Diaminobenzidine (DAB) was performed using a DAB chromogen system (Dako, Carpinteria, CA, USA).

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Article Snippet: 3,3′-Diaminobenzidine (DAB) was used as chromogen (DAKO).

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Article Snippet: Since endogenous brown colored material of the pineal gland tissue interfered with the analysis of small poly(GP), poly(GR), poly(PR) and pTDP-43 inclusions visualized by 3,3′-diaminobenzidine (DAB), these inclusions were also visualized by a Fast Red-type chromogen using the Dako REAL Detection System (K5005, Agilent, Santa Clara, CA, USA) for poly(GR) and poly(PR) or the Bond Polymer Refine Red Detection kit (DS9800, Leica Biosystems) for poly(GP) and pTDP-43.

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Article Snippet: The sections were next incubated at 4°C for 18h with the primary antibody (rabbit anti-GFAP, Dako, diluted as 1: 30.000), and the next day the signal was visualized with an anti-rabbit peroxidase polymer detection system adsorbed for mouse immunoglobulins (Nikirei-Bioscience, Tokyo, Japan) and 3,3’-diaminobenzidine (DAB) (Dako, Glostrup, Denmark).

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Article Snippet: Three µm sections of each paraffin-embedded biopsy were stained for the qualitative detection of p16ink4a protein with CINtec p16 Histology (Ventana Medical System, Oro Valley, AZ, USA) according to standard protocols and were visualized using 3.3’-diaminobenzidine (DAB) (DAKO, Glostrup, Denmark) with an exposure time of 3 minutes.

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Article Snippet: Indirect immunohistochemistry was performed using the EnVisionSystem, horse-radish peroxidase (HRP) and 3′3′-diaminobenzidine (DAB) (Dako, Glostrup, Denmark) method, as described previously .

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Article Snippet: Sections were washed incubated with 3,3’-diaminobenzidine (DAB) (DAKO), washed and counterstained with haematoxylin.

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Article Snippet: After washing with PBS, blocked with 10% NGS in TBS for 10 min, the secondary antibody (goat anti-rabbit IgG (H+L), horseradish peroxidase conjugate, Abcam) was applied for 30 min and the signals visualized using 0.75 mg/ml 3,3’-diaminobenzidine (DAB) with 0.015% hydrogen peroxide in Tris buffer. (DAB/DAB+ Chromogen Solution, Dako).

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Article Snippet: The bound antibodies were visualized using 3,3′-diaminobenzidine (DAB) as a chromogen (Dako, Glostrup, Denmark), and the slides were counterstained with hematoxylin.

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Article Snippet: At this point, the sections were incubated in 3,3’-Diaminobenzidine (DAB) (Dako North America, Inc.) for 5 minutes and counterstained in hematoxylin for 30 seconds.

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Article Snippet: The slides were rinsed with a TBS series and visualized after a 10-min incubation of liquid 3,3′-diaminobenzidine (DAB) in buffered substrate (Dako) for 10 min.

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Article Snippet: As previously described ( ; ), cells were stained with the peroxidase antiperoxidase method ( ) using 3,3′-diaminobenzidine (DAB) as a chromogen (Dako Corporation, Carpinteria, CA, USA).

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Article Snippet: The chromogenic substrate 3,3′-diaminobenzidine (DAB) (Dako Corp.) was used to localize antigen-antibody complexes.

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Article Snippet: Furthermore, 3,3′-diaminobenzidine (DAB) (DAKO, Shanghai, China) was administered to develop the peroxidase reaction, and the tissue sections were also stained with hematoxylin.

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Article Snippet: The tissue sections were again washed in wash buffer, and 3,3-diaminobenzidine (DAB) (#K5207, Agilent Dako, Santa Clara, CA, USA) was applied.

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Article Snippet: The reactions were revealed by applying 3,3’-diaminobenzidine (DAB) in chromogen solution (Dako; Carpinteria, California, USA).

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Article Snippet: In further steps the sections were washed in TRIS buffer and chromogen solution of 3,3’-diaminobenzidine (DAB), prepared according to the manufacturer’s instructions (Dako, Denmark), was added.

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Article Snippet: Color development was attained by exposing tissue to one of three substrate-chromagens, either 3,3'-diaminobenzidine (DAB) for 5 min; or 6-bromo-2-hydroxyl-3-naphtholic acid (Histomark Red, Kirkegaard and Perry, Gaithersburg, MD) for 50 minutes in the dark; or DAKO Permanent Red for 50 minutes in the dark.

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Article Snippet: The sections were incubated with rabbit anti-CEBPβ antibodies (1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4°C overnight in a humidified chamber, then incubated with HRP-conjugated goat anti-rabbit antibodies (1:500; Proteintech Group) at 37°C for 30 min, and then detected with 3,3′-diaminobenzidine (DAB) (Dako, Glostrup, Denmark) and counterstained with Mayer’s hematoxylin solution (Sigma-Aldrich).

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Article Snippet: Antibodies were revealed with EnVision system using 3,3-diaminobenzidine (DAB) (Dako, Trappes, France) or phtalocyanin red (Histomark Red, Kirkegaard and Perry Laboratories, Gaithersburg, MD).

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    Agilent technologies 3 3 diaminobenzidine dab substrate
    Image analysis workflow. Quantification of mesenchymal stromal cell/pericyte markers was performed using the CellProfiler image analysis software, and workflows were established for each respective antibody (representative CD146 and CD73 are shown). Fields of view were taken from the original image (Raw) and using the program threshold algorithms <t>DAB</t> stained regions (DAB regions) and nuclei (Nuclei) were defined (colored objects were arbitrarily assigned). Pixel area was determined from the colored segments detected in the DAB regions and nuclei. The images represent individual areas detected with either DAB or nuclei staining. These identified regions were subsequently overlaid on the original field of view to allow for validation (Overlay). x‐ and y ‐axes represent pixel co‐ordinates of the original images. Abbreviation: DAB, <t>3,3′‐diaminobenzidine</t>
    3 3 Diaminobenzidine Dab Substrate, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab substrate/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab substrate - by Bioz Stars, 2021-03
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    99
    Agilent technologies 3 3 diaminobenzidine
    NMR skin histomorphology and wound healing. ( A, B, D ) HE, Sirius Red and Masson’s Trichrome staining photomicrographs on sections of middle-aged (A) and young (Y) NMR back skin. ( C ) Histograms showing the epidermal and dermal thickness (μm) of middle-aged and young NMR back skin. ***p≤0.0001 in Mann-Whitney U test. n=4 animals per group. ( E-N ) Hyaluronic acid, Keratin 14, Keratin 10, Loricrin, Filaggrin, Laminin 5, ITGB4, Collagen I, Ki67, and p16 photomicrographs on sections of middle-aged and young NMR back skin. Cells were stained with either Alexa 546 or Alexa 488 fluorochromes. For hyaluronic acid, cells were stained with <t>3,3’-diaminobenzidine.</t> Negative controls were performed in parallel with the samples substituting the primary antibody with the equivalent isotype. Histogram showed the number of Ki67+ cells per surface in both age group. Scale bar = 50 or 100 μm. *represents differences between A and Y animals. Bars: SEM. ***p≤0.0001 in Mann-Whitney U test. n=4 animals per group. ( O ) Photomicrographs of back skin wounds for middle-aged and young animals from day 0 to day 22 post wounding. ( P ) Wound closure rate (% of wound surface at day 0) from day 4 to day 22 post wounding. No statistical difference was found the 2 groups using Mann-Whitney U test. n=4 animals per group.
    3 3 Diaminobenzidine, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Image analysis workflow. Quantification of mesenchymal stromal cell/pericyte markers was performed using the CellProfiler image analysis software, and workflows were established for each respective antibody (representative CD146 and CD73 are shown). Fields of view were taken from the original image (Raw) and using the program threshold algorithms DAB stained regions (DAB regions) and nuclei (Nuclei) were defined (colored objects were arbitrarily assigned). Pixel area was determined from the colored segments detected in the DAB regions and nuclei. The images represent individual areas detected with either DAB or nuclei staining. These identified regions were subsequently overlaid on the original field of view to allow for validation (Overlay). x‐ and y ‐axes represent pixel co‐ordinates of the original images. Abbreviation: DAB, 3,3′‐diaminobenzidine

    Journal: Stem Cells Translational Medicine

    Article Title: Dynamic Changes in Brain Mesenchymal Perivascular Cells Associate with Multiple Sclerosis Disease Duration, Active Inflammation, and Demyelination

    doi: 10.1002/sctm.17-0028

    Figure Lengend Snippet: Image analysis workflow. Quantification of mesenchymal stromal cell/pericyte markers was performed using the CellProfiler image analysis software, and workflows were established for each respective antibody (representative CD146 and CD73 are shown). Fields of view were taken from the original image (Raw) and using the program threshold algorithms DAB stained regions (DAB regions) and nuclei (Nuclei) were defined (colored objects were arbitrarily assigned). Pixel area was determined from the colored segments detected in the DAB regions and nuclei. The images represent individual areas detected with either DAB or nuclei staining. These identified regions were subsequently overlaid on the original field of view to allow for validation (Overlay). x‐ and y ‐axes represent pixel co‐ordinates of the original images. Abbreviation: DAB, 3,3′‐diaminobenzidine

    Article Snippet: Bound antibody was detected using the 3,3′‐diaminobenzidine (DAB) substrate (DAKO) for HRP and the signal developed for an optimized time period.

    Techniques: Software, Staining

    Tonsil sections stained with antibodies against (a) CD20 (diluted 1 : 1500), (b) CD3 (diluted 1 : 100), (c) Toll-like receptor (TLR)1 (diluted 1 : 50), (d) TLR2 (diluted 1 : 50), (e) TLR7 (diluted 1 : 50) and (f) TLR9 (diluted 1 : 50), were localized using 3,3′-diaminobenzidine (DAB), which stains tissues brown, and analysed by microscopy (magnification ×40–100).

    Journal: Immunology

    Article Title: A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by Pam3CSK4, R-837 and CpG-2006 stimulation

    doi: 10.1111/j.1365-2567.2006.02392.x

    Figure Lengend Snippet: Tonsil sections stained with antibodies against (a) CD20 (diluted 1 : 1500), (b) CD3 (diluted 1 : 100), (c) Toll-like receptor (TLR)1 (diluted 1 : 50), (d) TLR2 (diluted 1 : 50), (e) TLR7 (diluted 1 : 50) and (f) TLR9 (diluted 1 : 50), were localized using 3,3′-diaminobenzidine (DAB), which stains tissues brown, and analysed by microscopy (magnification ×40–100).

    Article Snippet: Subsequently, HRP-labelled goat anti-mouse or goat anti-rabbit polymer (Dako) was incubated with the sections for 30 min, followed by 3,3′-diaminobenzidine (DAB) substrate-chromogen (Dako) for 5–10 min. On some occasions, sections were counterstained with Mayer's haematoxylin.

    Techniques: Staining, Microscopy

    NMR skin histomorphology and wound healing. ( A, B, D ) HE, Sirius Red and Masson’s Trichrome staining photomicrographs on sections of middle-aged (A) and young (Y) NMR back skin. ( C ) Histograms showing the epidermal and dermal thickness (μm) of middle-aged and young NMR back skin. ***p≤0.0001 in Mann-Whitney U test. n=4 animals per group. ( E-N ) Hyaluronic acid, Keratin 14, Keratin 10, Loricrin, Filaggrin, Laminin 5, ITGB4, Collagen I, Ki67, and p16 photomicrographs on sections of middle-aged and young NMR back skin. Cells were stained with either Alexa 546 or Alexa 488 fluorochromes. For hyaluronic acid, cells were stained with 3,3’-diaminobenzidine. Negative controls were performed in parallel with the samples substituting the primary antibody with the equivalent isotype. Histogram showed the number of Ki67+ cells per surface in both age group. Scale bar = 50 or 100 μm. *represents differences between A and Y animals. Bars: SEM. ***p≤0.0001 in Mann-Whitney U test. n=4 animals per group. ( O ) Photomicrographs of back skin wounds for middle-aged and young animals from day 0 to day 22 post wounding. ( P ) Wound closure rate (% of wound surface at day 0) from day 4 to day 22 post wounding. No statistical difference was found the 2 groups using Mann-Whitney U test. n=4 animals per group.

    Journal: bioRxiv

    Article Title: Epidermal stem cell compartment remains unaffected through aging in naked mole-rats

    doi: 10.1101/2020.11.13.381061

    Figure Lengend Snippet: NMR skin histomorphology and wound healing. ( A, B, D ) HE, Sirius Red and Masson’s Trichrome staining photomicrographs on sections of middle-aged (A) and young (Y) NMR back skin. ( C ) Histograms showing the epidermal and dermal thickness (μm) of middle-aged and young NMR back skin. ***p≤0.0001 in Mann-Whitney U test. n=4 animals per group. ( E-N ) Hyaluronic acid, Keratin 14, Keratin 10, Loricrin, Filaggrin, Laminin 5, ITGB4, Collagen I, Ki67, and p16 photomicrographs on sections of middle-aged and young NMR back skin. Cells were stained with either Alexa 546 or Alexa 488 fluorochromes. For hyaluronic acid, cells were stained with 3,3’-diaminobenzidine. Negative controls were performed in parallel with the samples substituting the primary antibody with the equivalent isotype. Histogram showed the number of Ki67+ cells per surface in both age group. Scale bar = 50 or 100 μm. *represents differences between A and Y animals. Bars: SEM. ***p≤0.0001 in Mann-Whitney U test. n=4 animals per group. ( O ) Photomicrographs of back skin wounds for middle-aged and young animals from day 0 to day 22 post wounding. ( P ) Wound closure rate (% of wound surface at day 0) from day 4 to day 22 post wounding. No statistical difference was found the 2 groups using Mann-Whitney U test. n=4 animals per group.

    Article Snippet: HA-binding protein staining was achieved with 1/100 strepavidin HRP (Perkin Elmer, Massachusetts, USA) solution for 30 min at room temperature and 3,3’-diaminobenzidine (Liquid DAB + substrate Chromagen System; Dako, Santa Clara, CA, USA) for color development.

    Techniques: Nuclear Magnetic Resonance, Staining, MANN-WHITNEY

    Representative images of GFAP+ astrocytes in the hippocampus of adult Long-Evans hooded male rats exposed to low-F − chow/RO-H 2 O or low-F − chow/20 ppm F − drinking water beginning on gestational day 6. ( a ) Suprapyramidal blade of the dentate gyrus. ( b ) CA1 pyramidal cell layer. Cells displayed normal process-bearing morphology with no evidence of hypertrophy. 3,3-diaminobenzidine staining (brown). Hematoxylin counterstain (blue) showed no disruption of the normal morphology of the hippocampal regions and no evidence of neuronal death. ( n = 6). Scale bar = 100 μm

    Journal: Neurotoxicity Research

    Article Title: An Evaluation of Neurotoxicity Following Fluoride Exposure from Gestational Through Adult Ages in Long-Evans Hooded Rats

    doi: 10.1007/s12640-018-9870-x

    Figure Lengend Snippet: Representative images of GFAP+ astrocytes in the hippocampus of adult Long-Evans hooded male rats exposed to low-F − chow/RO-H 2 O or low-F − chow/20 ppm F − drinking water beginning on gestational day 6. ( a ) Suprapyramidal blade of the dentate gyrus. ( b ) CA1 pyramidal cell layer. Cells displayed normal process-bearing morphology with no evidence of hypertrophy. 3,3-diaminobenzidine staining (brown). Hematoxylin counterstain (blue) showed no disruption of the normal morphology of the hippocampal regions and no evidence of neuronal death. ( n = 6). Scale bar = 100 μm

    Article Snippet: Sections were incubated with rabbit anti-cow glial fibrillary acidic protein (Dako GFAP; 1:7000; RT; 30 min; Agilent Technologies, Carpinteria, CA) then incubated with biotinylated goat anti-rabbit IgG (1:500; Vector-Labs) and detected with Vectastain Elite ABC R.T.U. (Vector Labs); 3,3-diaminobenzidine (DAB, Agilent Technologies).

    Techniques: Staining

    Representative images of Iba-1+ microglia in the hippocampus of adult Long-Evans hooded rats exposed to low-F − chow/RO-H 2 O or low-F − chow/20 ppm F − drinking water beginning on gestational day 6. ( a ) Suprapyramidal blade of the dentate gyrus. ( b ) CA1 pyramidal cell layer. Cells displayed normal process-bearing morphology with no evidence of reactivity or activation. 3,3-diaminobenzidine staining (brown). Hematoxylin counterstain (blue) ( n = 6). Scale bar = 100 μm

    Journal: Neurotoxicity Research

    Article Title: An Evaluation of Neurotoxicity Following Fluoride Exposure from Gestational Through Adult Ages in Long-Evans Hooded Rats

    doi: 10.1007/s12640-018-9870-x

    Figure Lengend Snippet: Representative images of Iba-1+ microglia in the hippocampus of adult Long-Evans hooded rats exposed to low-F − chow/RO-H 2 O or low-F − chow/20 ppm F − drinking water beginning on gestational day 6. ( a ) Suprapyramidal blade of the dentate gyrus. ( b ) CA1 pyramidal cell layer. Cells displayed normal process-bearing morphology with no evidence of reactivity or activation. 3,3-diaminobenzidine staining (brown). Hematoxylin counterstain (blue) ( n = 6). Scale bar = 100 μm

    Article Snippet: Sections were incubated with rabbit anti-cow glial fibrillary acidic protein (Dako GFAP; 1:7000; RT; 30 min; Agilent Technologies, Carpinteria, CA) then incubated with biotinylated goat anti-rabbit IgG (1:500; Vector-Labs) and detected with Vectastain Elite ABC R.T.U. (Vector Labs); 3,3-diaminobenzidine (DAB, Agilent Technologies).

    Techniques: Activation Assay, Staining