3 3 diaminobenzidine dab (Agilent technologies)
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3 3 Diaminobenzidine Dab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Circadian sleep/wake-associated cells show dipeptide repeat protein aggregates in C9orf72-related ALS and FTLD cases"
Article Title: Circadian sleep/wake-associated cells show dipeptide repeat protein aggregates in C9orf72-related ALS and FTLD cases
Journal: Acta Neuropathologica Communications
doi: 10.1186/s40478-019-0845-9

Figure Legend Snippet: Neuropathological investigation of the SCN-related neurons in the hypothalamus of C9orf72 and non C9orf72 cases. VIP-ir neurons in the hypothalamus at the level of the SON and PVN were referred to as SCN-related neurons (case nonC9–16) ( a ). The inset shows VIP-immunostaining in neurons and fibers ( a ). Poly(GA) pathology (visualized by Fast red) was observed in few VIP-ir SCN-related neurons (visualized in brown with DAB) in half of the C9orf72 cases (case C9–3) ( b ). Inset b1 shows a magnification of a SCN-related neuron with poly(GA) pathology; inset b2 shows a magnification of a VIP-negative neuron with a poly(GA) inclusion. Poly(GA) inclusions (visualized by Fast red) were observed in the area of the SCN-related neurons and fibers (encircled) visualized in brown by DAB ( c ), however, more poly(GA) inclusions are observed in the area surrounding the SCN-related neurons and fibers (case C9–1) ( c ). SCN-related neurons were usually spared from pTDP-43 pathology. In some cases, pTDP-43 pathology (black arrows) (visualized by Fast red) was observed in VIP-negative neurons located in between the SCN-related neurons (arrowhead) and fibers (case nonC9–1) ( d ). The inset in d shows a magnification of a VIP-negative neuron with pTDP-43 pathology. Scale bars represent 1000 μm in a , 100 μm in b , 200 μm in c , 50 μm in d and inset of a , and 5 μm in insets of b and d . VIP-ir stands for vasoactive intestinal peptide-immunoreactive; SCN, suprachiasmatic nucleus; DAB, 3,3′-diaminobenzidine
Techniques Used: Immunostaining

Figure Legend Snippet: DPR and pTDP-43 pathology in the pineal gland of C9orf72 and non C9orf72 cases. Poly(GA) ( a ) and poly(GP) ( b ) inclusions were observed in the pineal gland of all C9orf72 cases. To a lesser extent, poly(GR) ( c ) and poly(PR) ( d ) inclusions were present in the pineal gland of C9orf72 cases. Panels a-d show immunohistochemical stainings of case C9–7. The insets show a magnification of the respective DPR inclusions. The chromogen used for visualization of poly(GP), poly(GR) and poly(PR) inclusions is a Fast Red-type chromogen, therefore, a red background color was obtained. Poly(GA) inclusions (visualized by DAB) are present in the melatonin-producing pinealocytes expressing synaptophysin (visualized by Fast red) shown here in case C9–1 ( e ). The inset of e shows a magnification of a synaptophysin-positive neuron with a poly(GA) inclusion. The pineal gland is devoid of pTDP-43 pathology (visualized by Fast red) ( f ). Endogenous brown colored material was observed in the tissue ( b-d, f ). Scale bar represents 50 μm, the scale bar of the insets represents 5 μm. DAB stands for 3,3′-diaminobenzidine
Techniques Used: Immunohistochemistry, Expressing
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