2mercaptoethanol  (Bio-Rad)

 
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  • 99
    Name:
    2 Mercaptoethanol
    Description:
    25 ml 98 pure 2 mercaptoethanol 14 2 M
    Catalog Number:
    1610710
    Price:
    None
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    Bio-Rad 2mercaptoethanol
    2 Mercaptoethanol
    25 ml 98 pure 2 mercaptoethanol 14 2 M
    https://www.bioz.com/result/2mercaptoethanol/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2mercaptoethanol - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Oligomannosidic glycans at Asn-110 are essential for secretion of human diamine oxidase
    Article Snippet: .. Cell lysates were obtained by resuspending 5 × 105 transiently transfected cells on day 2 in 250 μl of 90 °C 1× sample buffer + 2-mercaptoethanol; 10 μl (20,000 cells) were loaded on 4–20% Mini-PROTEAN® TGXTM gels (Bio-Rad). β-Actin was used as loading control. .. 1:5000 dilutions of β-actin mAB AC-15 (Invitrogen, AM4302) and IRDye® 680RD goat anti-mouse IgG (H + L) (Li-Cor, 925-68070) were used.

    Mouse Assay:

    Article Title: Characterization of immune responses following intramuscular DNA immunization with the MOMP gene of Chlamydia trachomatis mouse pneumonitis strain
    Article Snippet: .. Western blot analysis was performed to identify anti-MOMP antibody in the sera of immunized mice., Briefly, purified MoPn EBs were lysed by boiling in sample buffer containing 2% sodium dodecyl sulphate (SDS) and 200 mm 2ME and subsequently electrophoresed in a 10% SDS–polyacrylamide gel (Bio-Rad, Hercules, CA). ..

    Purification:

    Article Title: Characterization of immune responses following intramuscular DNA immunization with the MOMP gene of Chlamydia trachomatis mouse pneumonitis strain
    Article Snippet: .. Western blot analysis was performed to identify anti-MOMP antibody in the sera of immunized mice., Briefly, purified MoPn EBs were lysed by boiling in sample buffer containing 2% sodium dodecyl sulphate (SDS) and 200 mm 2ME and subsequently electrophoresed in a 10% SDS–polyacrylamide gel (Bio-Rad, Hercules, CA). ..

    other:

    Article Title: Electrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces
    Article Snippet: 2-mercaptoethanol was purchased from BioRad (Hercules, CA, USA).

    Western Blot:

    Article Title: Characterization of immune responses following intramuscular DNA immunization with the MOMP gene of Chlamydia trachomatis mouse pneumonitis strain
    Article Snippet: .. Western blot analysis was performed to identify anti-MOMP antibody in the sera of immunized mice., Briefly, purified MoPn EBs were lysed by boiling in sample buffer containing 2% sodium dodecyl sulphate (SDS) and 200 mm 2ME and subsequently electrophoresed in a 10% SDS–polyacrylamide gel (Bio-Rad, Hercules, CA). ..

    Silver Staining:

    Article Title: The Membrane M Protein Carboxy Terminus Binds to Transmissible Gastroenteritis Coronavirus Core and Contributes to Core Stability
    Article Snippet: .. The M-to-N molar ratio was estimated after KCl, NaCl, guanidine isothiocyanate, Triton X-100, and 2-mercaptoethanol treatments by SDS-PAGE, silver staining , and band densitometry using a Gel Documentation System 2000 (Bio-Rad). .. For Western blot analysis, the proteins were transferred to a nitrocellulose membrane with a Bio-Rad Mini Protean II electroblotting apparatus at 150 mA for 2 h in 25 mM Tris–192 mM glycine buffer (pH 8.3) containing 20% methanol.

    SDS Page:

    Article Title: The Membrane M Protein Carboxy Terminus Binds to Transmissible Gastroenteritis Coronavirus Core and Contributes to Core Stability
    Article Snippet: .. The M-to-N molar ratio was estimated after KCl, NaCl, guanidine isothiocyanate, Triton X-100, and 2-mercaptoethanol treatments by SDS-PAGE, silver staining , and band densitometry using a Gel Documentation System 2000 (Bio-Rad). .. For Western blot analysis, the proteins were transferred to a nitrocellulose membrane with a Bio-Rad Mini Protean II electroblotting apparatus at 150 mA for 2 h in 25 mM Tris–192 mM glycine buffer (pH 8.3) containing 20% methanol.

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  • 99
    Bio-Rad 2mercaptoethanol
    2mercaptoethanol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2mercaptoethanol/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2mercaptoethanol - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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