staphylococcus haemolyticus  (ATCC)


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    Structured Review

    ATCC staphylococcus haemolyticus
    ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. <t>haemolyticus</t> A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.
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    Images

    1) Product Images from "Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide"

    Article Title: Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide

    Journal: Antibiotics

    doi: 10.3390/antibiotics10070879

    ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. haemolyticus A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.
    Figure Legend Snippet: ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. haemolyticus A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.

    Techniques Used: Incubation, Expressing

    2) Product Images from "Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection"

    Article Title: Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection

    Journal: mBio

    doi: 10.1128/mBio.00296-16

    S. aureus displays better anaerobic growth than CoNS. Anaerobic growth of S. aureus (SA) COL and LAC, S. epidermidis (SE) RP62A, S. haemolyticus (SH) ATCC 29970, and S. saprophyticus (SS) ATCC 15305 in TSB (A) and CDM plus 25 mM glucose (C) ( n = 3). Corresponding average growth rates for TSB and CDM plus glucose are displayed in panels B and D, respectively ( n = 3; error bars show the pooled standard error of the mean). Growth rates were calculated from 2 to 4 h ( S. aureus LAC) and 3 to 5 h ( S. aureus COL, S. epidermidis , S. haemolyticus , and S. saprophyticus ) in TSB and from 2 to 8 h in CDM plus 25 mM glucose. Statistical significance was calculated with a Student two-sided t test (***, P ≤ 0.001). Abs, absorbance.
    Figure Legend Snippet: S. aureus displays better anaerobic growth than CoNS. Anaerobic growth of S. aureus (SA) COL and LAC, S. epidermidis (SE) RP62A, S. haemolyticus (SH) ATCC 29970, and S. saprophyticus (SS) ATCC 15305 in TSB (A) and CDM plus 25 mM glucose (C) ( n = 3). Corresponding average growth rates for TSB and CDM plus glucose are displayed in panels B and D, respectively ( n = 3; error bars show the pooled standard error of the mean). Growth rates were calculated from 2 to 4 h ( S. aureus LAC) and 3 to 5 h ( S. aureus COL, S. epidermidis , S. haemolyticus , and S. saprophyticus ) in TSB and from 2 to 8 h in CDM plus 25 mM glucose. Statistical significance was calculated with a Student two-sided t test (***, P ≤ 0.001). Abs, absorbance.

    Techniques Used:

    3) Product Images from "Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections"

    Article Title: Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections

    Journal: BMC Microbiology

    doi: 10.1186/s12866-021-02164-8

    A: HRMA Derivative Melting Curves and B: HRMA Aligned Melting Curves for some of Reference strains 1a: V1 region of K. pneumonia ATCC 700603, 1b: V3 region of K. pneumonia ATCC 700603, 1c: V6 region of K. pneumonia ATCC 700603, 2a: V1 region of S. aureus ATCC 25923 , 2b: V3 region of S. aureus ATCC 25923, 2c: V6 region of S. aureus ATCC 25923, 3a: V1 region of B. melitensis 16 M ATCC 23456, 3b: V3 region of B. melitensis 16 M ATCC 23456, 3c: V6 region of B. melitensis 16 M ATCC 23456
    Figure Legend Snippet: A: HRMA Derivative Melting Curves and B: HRMA Aligned Melting Curves for some of Reference strains 1a: V1 region of K. pneumonia ATCC 700603, 1b: V3 region of K. pneumonia ATCC 700603, 1c: V6 region of K. pneumonia ATCC 700603, 2a: V1 region of S. aureus ATCC 25923 , 2b: V3 region of S. aureus ATCC 25923, 2c: V6 region of S. aureus ATCC 25923, 3a: V1 region of B. melitensis 16 M ATCC 23456, 3b: V3 region of B. melitensis 16 M ATCC 23456, 3c: V6 region of B. melitensis 16 M ATCC 23456

    Techniques Used:

    HRMA Derivative Melting Curves of Reference strains for V1, V3, V6 regions of S. aureus ATCC 25923 and S. epidermidis strain Bjg (MK 516263) 1a: V1 region of S. aureus , 1b: V1 region of S. epidermidis , 2a: V6 region of S. aureus , 2b: V6 region of S. epidermidis, 3a: V3 region of S. aureus , 3b: V3 region of S. epidermidis
    Figure Legend Snippet: HRMA Derivative Melting Curves of Reference strains for V1, V3, V6 regions of S. aureus ATCC 25923 and S. epidermidis strain Bjg (MK 516263) 1a: V1 region of S. aureus , 1b: V1 region of S. epidermidis , 2a: V6 region of S. aureus , 2b: V6 region of S. epidermidis, 3a: V3 region of S. aureus , 3b: V3 region of S. epidermidis

    Techniques Used:

    a : HRMA Derivative Melting Curves and b : HRMA Aligned Melting Curves for V1, V3, V6 regions of S. aureus ATCC 25923 and S. aureus isolated from clinical (PJI) specimens Ref-V1: Reference strain-V1 region, S-V1: Clinical specimen-V1 region, Ref-V3: Reference strain-V3 region, S-V3: Clinical specimen-V3 region, Ref-V6: Reference strain-V6 region, S-V6: Clinical specimen- V6 region
    Figure Legend Snippet: a : HRMA Derivative Melting Curves and b : HRMA Aligned Melting Curves for V1, V3, V6 regions of S. aureus ATCC 25923 and S. aureus isolated from clinical (PJI) specimens Ref-V1: Reference strain-V1 region, S-V1: Clinical specimen-V1 region, Ref-V3: Reference strain-V3 region, S-V3: Clinical specimen-V3 region, Ref-V6: Reference strain-V6 region, S-V6: Clinical specimen- V6 region

    Techniques Used: Isolation

    Distribution of detected microorganisms from PJIs MRSA: Methicillin-resistant Staphylococcus aureus, MSSA: Methicillin-susceptible Staphylococcus aureus , MRSE: Methicillin-resistant Staphylococcus epidermidis , MSSE: Methicillin-susceptible Staphylococcus epidermidis , MR- S. haemolyticus: Methicillin-resistant Staphylococcus haemolyticus , Other MR-CoNS: Other Methicillin-Resistant Coagulase-Negative Staphylococci, Other MS-CoNS: Other Methicillin-susceptible Coagulase-Negative Staphylococci, VSE : Vancomycin-sensitive Enterococcus faecalis , VRE: Vancomycin-resistant Enterococcus faecalis , C. simulans : Corynebacterium simulans , D.incerta: Desemzia incerta, N.dassonville: Nocardiopsis dassonville, C.avidum: Cutibacterium avidum, F.magna : Finegoldia magna , B . melitensis : Brucella melitensis , E.coli : Escherichia coli, P.mirabilis: Proteus mirabilis, E.tarda: Edwardsiella tarda, P.aeruginosa: Pseudomonas aeruginosa, P.stutzeri: Pseudomonas stutzeri, P.oryzihabitans: Pseudomonas oryzihabitans, L.adecarboxylata: Leclercia adecarboxylata
    Figure Legend Snippet: Distribution of detected microorganisms from PJIs MRSA: Methicillin-resistant Staphylococcus aureus, MSSA: Methicillin-susceptible Staphylococcus aureus , MRSE: Methicillin-resistant Staphylococcus epidermidis , MSSE: Methicillin-susceptible Staphylococcus epidermidis , MR- S. haemolyticus: Methicillin-resistant Staphylococcus haemolyticus , Other MR-CoNS: Other Methicillin-Resistant Coagulase-Negative Staphylococci, Other MS-CoNS: Other Methicillin-susceptible Coagulase-Negative Staphylococci, VSE : Vancomycin-sensitive Enterococcus faecalis , VRE: Vancomycin-resistant Enterococcus faecalis , C. simulans : Corynebacterium simulans , D.incerta: Desemzia incerta, N.dassonville: Nocardiopsis dassonville, C.avidum: Cutibacterium avidum, F.magna : Finegoldia magna , B . melitensis : Brucella melitensis , E.coli : Escherichia coli, P.mirabilis: Proteus mirabilis, E.tarda: Edwardsiella tarda, P.aeruginosa: Pseudomonas aeruginosa, P.stutzeri: Pseudomonas stutzeri, P.oryzihabitans: Pseudomonas oryzihabitans, L.adecarboxylata: Leclercia adecarboxylata

    Techniques Used:

    4) Product Images from "Detection of low numbers of bacterial cells in pharmaceutical drug product using Raman Spectroscopy and PLS-DA multivariate analysis"

    Article Title: Detection of low numbers of bacterial cells in pharmaceutical drug product using Raman Spectroscopy and PLS-DA multivariate analysis

    Journal: bioRxiv

    doi: 10.1101/2022.04.26.489535

    Detection of low bacterial concentrations in vegetative forms. Raman spectra from 10 spiked samples (SS) and 10 negative controls (NC) were collected and analyzed using a three-component partial least squared with discriminant analysis (PLS-DA) model. Classification and score plots obtained in components number 1 and 2 of (A-B) SS with 50 CFU/ml of S. enterica (SS-50-SE); (C-D) SS with 10 CFU/ml of S. enterica (SS-10-SE); (E-F) SS with 50 CFU/ml of S. haemolyticus (SS-50-SH); (G-H) SS with 10 CFU/ml of S. haemolyticus (SS-10-SH).
    Figure Legend Snippet: Detection of low bacterial concentrations in vegetative forms. Raman spectra from 10 spiked samples (SS) and 10 negative controls (NC) were collected and analyzed using a three-component partial least squared with discriminant analysis (PLS-DA) model. Classification and score plots obtained in components number 1 and 2 of (A-B) SS with 50 CFU/ml of S. enterica (SS-50-SE); (C-D) SS with 10 CFU/ml of S. enterica (SS-10-SE); (E-F) SS with 50 CFU/ml of S. haemolyticus (SS-50-SH); (G-H) SS with 10 CFU/ml of S. haemolyticus (SS-10-SH).

    Techniques Used:

    5) Product Images from "Whole-Genome Sequencing of Staphylococcus haemolyticus Uncovers the Extreme Plasticity of Its Genome and the Evolution of Human-Colonizing Staphylococcal Species"

    Article Title: Whole-Genome Sequencing of Staphylococcus haemolyticus Uncovers the Extreme Plasticity of Its Genome and the Evolution of Human-Colonizing Staphylococcal Species

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.187.21.7292-7308.2005

    Spontaneous phenotypic mutants of S.haemolyticus JCSC1435 arose due to oriC environ rearrangement. (a) PFGE banding patterns of mutant strains compared with JCSC1435. (b) Circular representation of JCSC1435 chromosome and the deletions in the mutant strains
    Figure Legend Snippet: Spontaneous phenotypic mutants of S.haemolyticus JCSC1435 arose due to oriC environ rearrangement. (a) PFGE banding patterns of mutant strains compared with JCSC1435. (b) Circular representation of JCSC1435 chromosome and the deletions in the mutant strains

    Techniques Used: Mutagenesis

    Frequent rearrangement of S.haemolyticus chromosome.
    Figure Legend Snippet: Frequent rearrangement of S.haemolyticus chromosome.

    Techniques Used:

    6) Product Images from "Comparative characterisation of human and ovine non-aureus staphylococci isolated in Sardinia (Italy) for antimicrobial susceptibility profiles and resistance genes"

    Article Title: Comparative characterisation of human and ovine non-aureus staphylococci isolated in Sardinia (Italy) for antimicrobial susceptibility profiles and resistance genes

    Journal: Epidemiology and Infection

    doi: 10.1017/S0950268821000212

    RFLP patterns obtained for 15 reference strains after digestion of the gap gene amplicon with Alu I. Fragments were separated by 12% PAGE gel. Lane M, marker VIII (Roche); lane 1, S. xylosus ATCC 29971; lane 2, S. saprophyticus ATCC 15305; lane 3, S. capitis ATCC 27840; lane 4, S. haemolyticus ATCC 29970; lane 5, S. simulans ATCC 27848, lane 6, S. warneri ATCC 27836; lane 7, S. arlettae ATCC 43957; lane 8, S. chromogenes ATCC 43764; lane 9, S. equorum ATCC 43958; lane 10, S. hominis ATCC 27844; lane 11, S. caprae ATCC 35538; lane 12, S. lentus ATCC 29070; lane 13, S. hyicus ATCC 11249, lane 14, S. epidermidis ATCC 14990 and lane 14, S. aureus ATCC 43300.
    Figure Legend Snippet: RFLP patterns obtained for 15 reference strains after digestion of the gap gene amplicon with Alu I. Fragments were separated by 12% PAGE gel. Lane M, marker VIII (Roche); lane 1, S. xylosus ATCC 29971; lane 2, S. saprophyticus ATCC 15305; lane 3, S. capitis ATCC 27840; lane 4, S. haemolyticus ATCC 29970; lane 5, S. simulans ATCC 27848, lane 6, S. warneri ATCC 27836; lane 7, S. arlettae ATCC 43957; lane 8, S. chromogenes ATCC 43764; lane 9, S. equorum ATCC 43958; lane 10, S. hominis ATCC 27844; lane 11, S. caprae ATCC 35538; lane 12, S. lentus ATCC 29070; lane 13, S. hyicus ATCC 11249, lane 14, S. epidermidis ATCC 14990 and lane 14, S. aureus ATCC 43300.

    Techniques Used: Amplification, Polyacrylamide Gel Electrophoresis, Marker

    7) Product Images from "Coagulase-Negative Staphylococci Clones Are Widely Distributed in the Hospital and Community"

    Article Title: Coagulase-Negative Staphylococci Clones Are Widely Distributed in the Hospital and Community

    Journal: Pathogens

    doi: 10.3390/pathogens10070792

    Dendrogram generated by Dice/UPGMA analysis (Bionumerics Applied Maths), from PFGE- SmaI profiles of S. haemolyticus isolated from nasal swabs ( A ), wounds ( B ), and blood cultures ( C ). Square brackets highlight the clusters ( > 80% similarity). Roman numerals represent the SCC mec type.
    Figure Legend Snippet: Dendrogram generated by Dice/UPGMA analysis (Bionumerics Applied Maths), from PFGE- SmaI profiles of S. haemolyticus isolated from nasal swabs ( A ), wounds ( B ), and blood cultures ( C ). Square brackets highlight the clusters ( > 80% similarity). Roman numerals represent the SCC mec type.

    Techniques Used: Generated, Isolation

    8) Product Images from "Global Expansion of Linezolid-Resistant Coagulase-Negative Staphylococci"

    Article Title: Global Expansion of Linezolid-Resistant Coagulase-Negative Staphylococci

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2021.661798

    Maximum-likelihood phylogeny of S. haemolyticus population ( n = 207) based on core-SNP alignment. Background color fill is matched to BAPS clustering (BAPS 1 to BAPS 4). Linezolid-restant isolates (LRS reports) isolates is marked triangles: from Russia (one isolate in current study) and the United States ( Tewhey et al., 2014 ). Annotation from inner to outer circle: presence mecA ; MLST data; outer bar chart is matched to the number of acquired resistance genes (from 0 to 13 genes, specific chromosomal mutations were not included). The following genes were screened: aac(6′)-aph(2″), aadD, ant(6)-Ia, ant(9)-Ia, aph(3′)-III, blaZ, cat, dfrG, ermA, ermC, cfr, fexA, fosB, fosD, fusB, fusC, lnu(A), lsa(B), mecA, mph(C), msr(A), str, tetC, tetK, tetL, tetM, vgaA, and vgaB.
    Figure Legend Snippet: Maximum-likelihood phylogeny of S. haemolyticus population ( n = 207) based on core-SNP alignment. Background color fill is matched to BAPS clustering (BAPS 1 to BAPS 4). Linezolid-restant isolates (LRS reports) isolates is marked triangles: from Russia (one isolate in current study) and the United States ( Tewhey et al., 2014 ). Annotation from inner to outer circle: presence mecA ; MLST data; outer bar chart is matched to the number of acquired resistance genes (from 0 to 13 genes, specific chromosomal mutations were not included). The following genes were screened: aac(6′)-aph(2″), aadD, ant(6)-Ia, ant(9)-Ia, aph(3′)-III, blaZ, cat, dfrG, ermA, ermC, cfr, fexA, fosB, fosD, fusB, fusC, lnu(A), lsa(B), mecA, mph(C), msr(A), str, tetC, tetK, tetL, tetM, vgaA, and vgaB.

    Techniques Used:

    9) Product Images from "Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland"

    Article Title: Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland

    Journal: Pathogens

    doi: 10.3390/pathogens10070877

    Agarose gel electrophoresis of multiplex PCR performed in order to identify bacterial species and detect the presence of the mecA gene: M—DNA molecular weight marker 100 bp (GeneRuler 100 bp DNA Ladder, ThermoFisher Scientific), 1- . aureus ATCC 25923 mecA (–) strain, 2- S. aureus ATCC 43300 mecA (+) strain, 3-clinical mecA (+) strain, 4- clinical Staphylococcus epidermidis strain, 5- Staphylococcus haemolyticus ATCC 29970 strain, 6– Staphylococcus epidermidis ATCC 12228 strain, 7-16-clinical Staphylococcus epidermidis strains. List of expected amplicons for (1) species identification: S. aureus -108 bp; S. epidermidis -124 bp; S. haemolyticus -271 bp; (2) mecA gene-154 bp.
    Figure Legend Snippet: Agarose gel electrophoresis of multiplex PCR performed in order to identify bacterial species and detect the presence of the mecA gene: M—DNA molecular weight marker 100 bp (GeneRuler 100 bp DNA Ladder, ThermoFisher Scientific), 1- . aureus ATCC 25923 mecA (–) strain, 2- S. aureus ATCC 43300 mecA (+) strain, 3-clinical mecA (+) strain, 4- clinical Staphylococcus epidermidis strain, 5- Staphylococcus haemolyticus ATCC 29970 strain, 6– Staphylococcus epidermidis ATCC 12228 strain, 7-16-clinical Staphylococcus epidermidis strains. List of expected amplicons for (1) species identification: S. aureus -108 bp; S. epidermidis -124 bp; S. haemolyticus -271 bp; (2) mecA gene-154 bp.

    Techniques Used: Agarose Gel Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Molecular Weight, Marker

    10) Product Images from "Identification and Characterization of Quorum-Quenching Activity of N-Acylhomoserine Lactonase from Coagulase-Negative Staphylococci"

    Article Title: Identification and Characterization of Quorum-Quenching Activity of N-Acylhomoserine Lactonase from Coagulase-Negative Staphylococci

    Journal: Antibiotics

    doi: 10.3390/antibiotics9080483

    N -Acylhomoserine lactone (AHL)-degrading activities of CNS strains. Full-grown cultures of CNS strains, Staphylococcus carnosus NBRC 109622 (Scar), Staphylococcus haemolyticus NBRC 109768 (Shae), Staphylococcus saprophyticus NBRC 102446 (Ssap), Staphylococcus sciuri American Type Culture Collection (ATCC) 29060 (Ssci1), S. sciuri 29061 (Ssci2), and S. sciuri StLB252 (Ssci3) were mixed with 20 μM N -hexanoyl- l -homoserine lactone (C6-HSL) or N -decanoyl- l -homoserine lactone (C10-HSL) and incubated at 37 °C for 4.5 and 9 h. The residual AHLs were detected using Chromobacterium violaceum CV026 (for C6-HSL) or VIR07 (for C10-HSL).
    Figure Legend Snippet: N -Acylhomoserine lactone (AHL)-degrading activities of CNS strains. Full-grown cultures of CNS strains, Staphylococcus carnosus NBRC 109622 (Scar), Staphylococcus haemolyticus NBRC 109768 (Shae), Staphylococcus saprophyticus NBRC 102446 (Ssap), Staphylococcus sciuri American Type Culture Collection (ATCC) 29060 (Ssci1), S. sciuri 29061 (Ssci2), and S. sciuri StLB252 (Ssci3) were mixed with 20 μM N -hexanoyl- l -homoserine lactone (C6-HSL) or N -decanoyl- l -homoserine lactone (C10-HSL) and incubated at 37 °C for 4.5 and 9 h. The residual AHLs were detected using Chromobacterium violaceum CV026 (for C6-HSL) or VIR07 (for C10-HSL).

    Techniques Used: Incubation

    Degradation of C6-HSL ( A ) and C10-HSL ( B ) by Escherichia coli DH5α harboring ahlS genes from the CNS strains. Full-grown cultures of E. coli DH5α harboring an empty vector pBBR1MCS5 (black) or pBBR1MCS5 containing the ahlS gene from S. carnosus NBRC 109622 (blue), S. haemolyticus NBRC 109768 (orange), S. saprophyticus NBRC 102446 (red), S. sciuri ATCC 29060 (yellow), S. sciuri 29061 (purple), and S. sciuri StLB252 (green) were inoculated into the Luria–Bertani (LB) medium containing 10 μM C6-HSL or C10-HSL and incubated at 37 °C for 3, 6, and 9 h. After incubation, the remaining AHLs in the culture supernatants were visualized by C. violaceum and calculated using the relationship equations based on the size of the purple-color zones. The data were reproduced at least three times and the error bars indicate standard deviations.
    Figure Legend Snippet: Degradation of C6-HSL ( A ) and C10-HSL ( B ) by Escherichia coli DH5α harboring ahlS genes from the CNS strains. Full-grown cultures of E. coli DH5α harboring an empty vector pBBR1MCS5 (black) or pBBR1MCS5 containing the ahlS gene from S. carnosus NBRC 109622 (blue), S. haemolyticus NBRC 109768 (orange), S. saprophyticus NBRC 102446 (red), S. sciuri ATCC 29060 (yellow), S. sciuri 29061 (purple), and S. sciuri StLB252 (green) were inoculated into the Luria–Bertani (LB) medium containing 10 μM C6-HSL or C10-HSL and incubated at 37 °C for 3, 6, and 9 h. After incubation, the remaining AHLs in the culture supernatants were visualized by C. violaceum and calculated using the relationship equations based on the size of the purple-color zones. The data were reproduced at least three times and the error bars indicate standard deviations.

    Techniques Used: Plasmid Preparation, Incubation

    Comparisons of AhlS from the CNS strains with the known AiiA-like AHL lactonases. The amino acid sequences of AhlS from S. carnosus NBRC 109622 (Scar), S. haemolyticus NBRC 109768 (Shae), S. saprophyticus NBRC 102446 (Ssap), and S. sciuri ATCC 29060 (Ssci) were compared to those of the AhlS from Solibacillus silvestris StLB046 (UniProt accession No. F2F233), AiiT from Thermaerobacter marianensis JCM 10246 (E6SI95), AiiA from Bacillus sp. 240B1 (Q9L8R8), AttM from Agrobacterium tumefaciens C58 (Q7D3U0), and Arthrobacter sp. IBN110 (Q7X3T2). Sequences were aligned using ClustalW and shaded using the GeneDoc software. The consensus amino acid sequence (HXHXDH) was marked with asterisks.
    Figure Legend Snippet: Comparisons of AhlS from the CNS strains with the known AiiA-like AHL lactonases. The amino acid sequences of AhlS from S. carnosus NBRC 109622 (Scar), S. haemolyticus NBRC 109768 (Shae), S. saprophyticus NBRC 102446 (Ssap), and S. sciuri ATCC 29060 (Ssci) were compared to those of the AhlS from Solibacillus silvestris StLB046 (UniProt accession No. F2F233), AiiT from Thermaerobacter marianensis JCM 10246 (E6SI95), AiiA from Bacillus sp. 240B1 (Q9L8R8), AttM from Agrobacterium tumefaciens C58 (Q7D3U0), and Arthrobacter sp. IBN110 (Q7X3T2). Sequences were aligned using ClustalW and shaded using the GeneDoc software. The consensus amino acid sequence (HXHXDH) was marked with asterisks.

    Techniques Used: Software, Sequencing

    11) Product Images from "Programmable molecular circuit discriminates multidrug-resistant bacteria"

    Article Title: Programmable molecular circuit discriminates multidrug-resistant bacteria

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2022.100379

    Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. haemolyticus , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​
    Figure Legend Snippet: Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. haemolyticus , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​

    Techniques Used: Fluorescence, Concentration Assay

    12) Product Images from "Pathogenesis of Staphylococcus haemolyticus on primary human skin fibroblast cells"

    Article Title: Pathogenesis of Staphylococcus haemolyticus on primary human skin fibroblast cells

    Journal: Virulence

    doi: 10.1080/21505594.2020.1809962

    Bacterial adhesion to PHSF cells as detected by Giemsa stain. After 3 h in culture, PHSF cells were challenged with different S. haemolyticus isolates. (a) Non-infected fibroblast cells, (b) Fibroblasts infected with S. haemolyticus isolate with low adhesion capacity, (c) Fibroblasts infected with S. haemolyticus isolate with high adhesion capacity, (d) Fibroblasts infected with the control S. haemolyticus strain (ATCC 29970) showing low adhesion capacity.
    Figure Legend Snippet: Bacterial adhesion to PHSF cells as detected by Giemsa stain. After 3 h in culture, PHSF cells were challenged with different S. haemolyticus isolates. (a) Non-infected fibroblast cells, (b) Fibroblasts infected with S. haemolyticus isolate with low adhesion capacity, (c) Fibroblasts infected with S. haemolyticus isolate with high adhesion capacity, (d) Fibroblasts infected with the control S. haemolyticus strain (ATCC 29970) showing low adhesion capacity.

    Techniques Used: Giemsa Stain, Infection

    Invasion of PHSF cells by FITC-labeled bacteria. Comparative histograms represent results of flow cytometry analysis of (a) control non-infected cells (mock), (b) poorly invasive strain of S. haemolyticus (SH1), (c) highly invasive strain of S. haemolyticus (SH10), (d) Control S. haemolyticus strain (ATCC 29970). The figure shows a representative of three independent experiments.
    Figure Legend Snippet: Invasion of PHSF cells by FITC-labeled bacteria. Comparative histograms represent results of flow cytometry analysis of (a) control non-infected cells (mock), (b) poorly invasive strain of S. haemolyticus (SH1), (c) highly invasive strain of S. haemolyticus (SH10), (d) Control S. haemolyticus strain (ATCC 29970). The figure shows a representative of three independent experiments.

    Techniques Used: Labeling, Flow Cytometry, Infection

    Flow cytometry analysis showing the cytotoxic effect of SH and SA on the PHSF cells. Cells were treated with indicated microbial products and stained after 24 h of incubation with Annexin V and PI. (a) Cells were infected with SH (upper panel), while in (b) cells were infected with SA (lower panel). (c) The mean of the cytotoxic activities of different tested strains on the PHSF cells ± SD. SH, S. haemolyticus ; SA, S. aureus.
    Figure Legend Snippet: Flow cytometry analysis showing the cytotoxic effect of SH and SA on the PHSF cells. Cells were treated with indicated microbial products and stained after 24 h of incubation with Annexin V and PI. (a) Cells were infected with SH (upper panel), while in (b) cells were infected with SA (lower panel). (c) The mean of the cytotoxic activities of different tested strains on the PHSF cells ± SD. SH, S. haemolyticus ; SA, S. aureus.

    Techniques Used: Flow Cytometry, Staining, Incubation, Infection

    Hemolytic activities ofclinical S. haemolyticus isolates compared to S. aureus and control S. haemolyticus (ATCC 29970) after 5 h of incubation. SH; S. haemolyticus , SA; S. aureus . Depicted are the mean of 3 independent experiments ± standard deviation.
    Figure Legend Snippet: Hemolytic activities ofclinical S. haemolyticus isolates compared to S. aureus and control S. haemolyticus (ATCC 29970) after 5 h of incubation. SH; S. haemolyticus , SA; S. aureus . Depicted are the mean of 3 independent experiments ± standard deviation.

    Techniques Used: Incubation, Standard Deviation

    The PHSF cells were challenged with S. haemolyticus at an MOI of 10 and examined with TEM. (a) After 15 min of infection, extracellular bacteria were observed (3600x) which were further magnified (14,000 x) in (b). (c) After 1 h of infection, bacteria invaded the cells and became engulfed (3600 x). (d) Initialization of phagocytosis. (e) After 90 min, S. haemolyticus entered the PHSF cells and become localized in vacuoles (3600 x). (f) Proliferating bacteria are shown inside the vacuole.
    Figure Legend Snippet: The PHSF cells were challenged with S. haemolyticus at an MOI of 10 and examined with TEM. (a) After 15 min of infection, extracellular bacteria were observed (3600x) which were further magnified (14,000 x) in (b). (c) After 1 h of infection, bacteria invaded the cells and became engulfed (3600 x). (d) Initialization of phagocytosis. (e) After 90 min, S. haemolyticus entered the PHSF cells and become localized in vacuoles (3600 x). (f) Proliferating bacteria are shown inside the vacuole.

    Techniques Used: Transmission Electron Microscopy, Infection

    Cytokine expression profile of PBMCs incubated with S. haemolyticus and S. aureus for 6 and 24 h . The figure shows the mean of 3 independent experiments ± standard error.
    Figure Legend Snippet: Cytokine expression profile of PBMCs incubated with S. haemolyticus and S. aureus for 6 and 24 h . The figure shows the mean of 3 independent experiments ± standard error.

    Techniques Used: Expressing, Incubation

    Percentage of internalized S. haemolyticus in the PHSF cells and the distribution of the fnBP genes.
    Figure Legend Snippet: Percentage of internalized S. haemolyticus in the PHSF cells and the distribution of the fnBP genes.

    Techniques Used:

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    ATCC staphylococcus haemolyticus
    ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. <t>haemolyticus</t> A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.
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    ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. haemolyticus A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.

    Journal: Antibiotics

    Article Title: Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide

    doi: 10.3390/antibiotics10070879

    Figure Lengend Snippet: ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. haemolyticus A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.

    Article Snippet: The following international reference strains were used as controls: S. epidermidis (ATCC 12228), S. epidermidis (ATCC 35983), S. haemolyticus (ATCC 29970), S. hominis (ATCC 27844), S. hominis subsp. novobiosepticus (ATCC 700237), S. lugdunensis (ATCC 700328), S. saprophyticus (ATCC 15305), and S. warneri (ATCC 10209).

    Techniques: Incubation, Expressing

    S. aureus displays better anaerobic growth than CoNS. Anaerobic growth of S. aureus (SA) COL and LAC, S. epidermidis (SE) RP62A, S. haemolyticus (SH) ATCC 29970, and S. saprophyticus (SS) ATCC 15305 in TSB (A) and CDM plus 25 mM glucose (C) ( n = 3). Corresponding average growth rates for TSB and CDM plus glucose are displayed in panels B and D, respectively ( n = 3; error bars show the pooled standard error of the mean). Growth rates were calculated from 2 to 4 h ( S. aureus LAC) and 3 to 5 h ( S. aureus COL, S. epidermidis , S. haemolyticus , and S. saprophyticus ) in TSB and from 2 to 8 h in CDM plus 25 mM glucose. Statistical significance was calculated with a Student two-sided t test (***, P ≤ 0.001). Abs, absorbance.

    Journal: mBio

    Article Title: Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection

    doi: 10.1128/mBio.00296-16

    Figure Lengend Snippet: S. aureus displays better anaerobic growth than CoNS. Anaerobic growth of S. aureus (SA) COL and LAC, S. epidermidis (SE) RP62A, S. haemolyticus (SH) ATCC 29970, and S. saprophyticus (SS) ATCC 15305 in TSB (A) and CDM plus 25 mM glucose (C) ( n = 3). Corresponding average growth rates for TSB and CDM plus glucose are displayed in panels B and D, respectively ( n = 3; error bars show the pooled standard error of the mean). Growth rates were calculated from 2 to 4 h ( S. aureus LAC) and 3 to 5 h ( S. aureus COL, S. epidermidis , S. haemolyticus , and S. saprophyticus ) in TSB and from 2 to 8 h in CDM plus 25 mM glucose. Statistical significance was calculated with a Student two-sided t test (***, P ≤ 0.001). Abs, absorbance.

    Article Snippet: To test whether this enhanced growth behavior occurs under other nonrespiratory conditions, we compared the anaerobic growth of S. aureus strains COL and LAC to that of S. epidermidis RP62A, S. haemolyticus ATCC 29970, and S. saprophyticus ATCC 15305 in a rich medium.

    Techniques:

    A: HRMA Derivative Melting Curves and B: HRMA Aligned Melting Curves for some of Reference strains 1a: V1 region of K. pneumonia ATCC 700603, 1b: V3 region of K. pneumonia ATCC 700603, 1c: V6 region of K. pneumonia ATCC 700603, 2a: V1 region of S. aureus ATCC 25923 , 2b: V3 region of S. aureus ATCC 25923, 2c: V6 region of S. aureus ATCC 25923, 3a: V1 region of B. melitensis 16 M ATCC 23456, 3b: V3 region of B. melitensis 16 M ATCC 23456, 3c: V6 region of B. melitensis 16 M ATCC 23456

    Journal: BMC Microbiology

    Article Title: Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections

    doi: 10.1186/s12866-021-02164-8

    Figure Lengend Snippet: A: HRMA Derivative Melting Curves and B: HRMA Aligned Melting Curves for some of Reference strains 1a: V1 region of K. pneumonia ATCC 700603, 1b: V3 region of K. pneumonia ATCC 700603, 1c: V6 region of K. pneumonia ATCC 700603, 2a: V1 region of S. aureus ATCC 25923 , 2b: V3 region of S. aureus ATCC 25923, 2c: V6 region of S. aureus ATCC 25923, 3a: V1 region of B. melitensis 16 M ATCC 23456, 3b: V3 region of B. melitensis 16 M ATCC 23456, 3c: V6 region of B. melitensis 16 M ATCC 23456

    Article Snippet: Reference strains used in this study were S. aureus ATCC 29213, S. aureus ATCC 25923, S. epidermidis strain Bjg (MK 516263), S. hemolyticus ATCC 29970, S. saprophyticus ATCC 15305, Micrococcus luteus ATCC 15307, Enterococcus faecalis ATCC 29212, Streptococcus sanguinis ATCC 10566, Finegoldia magna ATCC 1766, Cutibacterium ATCC 25573, Haemophilus influenza ATCC 49766, Pseudomonas aeruginosa 27,853, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 700603, Proteus mirabilis ATC 43071, Acinetobacter baumannii ATCC 19606, Brucella melitensis 16 M ATCC 23456, Mycobacterium tuberculosis H37Rv, Corynebacterium simulans strain BJd (MK 516260).

    Techniques:

    HRMA Derivative Melting Curves of Reference strains for V1, V3, V6 regions of S. aureus ATCC 25923 and S. epidermidis strain Bjg (MK 516263) 1a: V1 region of S. aureus , 1b: V1 region of S. epidermidis , 2a: V6 region of S. aureus , 2b: V6 region of S. epidermidis, 3a: V3 region of S. aureus , 3b: V3 region of S. epidermidis

    Journal: BMC Microbiology

    Article Title: Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections

    doi: 10.1186/s12866-021-02164-8

    Figure Lengend Snippet: HRMA Derivative Melting Curves of Reference strains for V1, V3, V6 regions of S. aureus ATCC 25923 and S. epidermidis strain Bjg (MK 516263) 1a: V1 region of S. aureus , 1b: V1 region of S. epidermidis , 2a: V6 region of S. aureus , 2b: V6 region of S. epidermidis, 3a: V3 region of S. aureus , 3b: V3 region of S. epidermidis

    Article Snippet: Reference strains used in this study were S. aureus ATCC 29213, S. aureus ATCC 25923, S. epidermidis strain Bjg (MK 516263), S. hemolyticus ATCC 29970, S. saprophyticus ATCC 15305, Micrococcus luteus ATCC 15307, Enterococcus faecalis ATCC 29212, Streptococcus sanguinis ATCC 10566, Finegoldia magna ATCC 1766, Cutibacterium ATCC 25573, Haemophilus influenza ATCC 49766, Pseudomonas aeruginosa 27,853, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 700603, Proteus mirabilis ATC 43071, Acinetobacter baumannii ATCC 19606, Brucella melitensis 16 M ATCC 23456, Mycobacterium tuberculosis H37Rv, Corynebacterium simulans strain BJd (MK 516260).

    Techniques:

    a : HRMA Derivative Melting Curves and b : HRMA Aligned Melting Curves for V1, V3, V6 regions of S. aureus ATCC 25923 and S. aureus isolated from clinical (PJI) specimens Ref-V1: Reference strain-V1 region, S-V1: Clinical specimen-V1 region, Ref-V3: Reference strain-V3 region, S-V3: Clinical specimen-V3 region, Ref-V6: Reference strain-V6 region, S-V6: Clinical specimen- V6 region

    Journal: BMC Microbiology

    Article Title: Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections

    doi: 10.1186/s12866-021-02164-8

    Figure Lengend Snippet: a : HRMA Derivative Melting Curves and b : HRMA Aligned Melting Curves for V1, V3, V6 regions of S. aureus ATCC 25923 and S. aureus isolated from clinical (PJI) specimens Ref-V1: Reference strain-V1 region, S-V1: Clinical specimen-V1 region, Ref-V3: Reference strain-V3 region, S-V3: Clinical specimen-V3 region, Ref-V6: Reference strain-V6 region, S-V6: Clinical specimen- V6 region

    Article Snippet: Reference strains used in this study were S. aureus ATCC 29213, S. aureus ATCC 25923, S. epidermidis strain Bjg (MK 516263), S. hemolyticus ATCC 29970, S. saprophyticus ATCC 15305, Micrococcus luteus ATCC 15307, Enterococcus faecalis ATCC 29212, Streptococcus sanguinis ATCC 10566, Finegoldia magna ATCC 1766, Cutibacterium ATCC 25573, Haemophilus influenza ATCC 49766, Pseudomonas aeruginosa 27,853, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 700603, Proteus mirabilis ATC 43071, Acinetobacter baumannii ATCC 19606, Brucella melitensis 16 M ATCC 23456, Mycobacterium tuberculosis H37Rv, Corynebacterium simulans strain BJd (MK 516260).

    Techniques: Isolation

    Distribution of detected microorganisms from PJIs MRSA: Methicillin-resistant Staphylococcus aureus, MSSA: Methicillin-susceptible Staphylococcus aureus , MRSE: Methicillin-resistant Staphylococcus epidermidis , MSSE: Methicillin-susceptible Staphylococcus epidermidis , MR- S. haemolyticus: Methicillin-resistant Staphylococcus haemolyticus , Other MR-CoNS: Other Methicillin-Resistant Coagulase-Negative Staphylococci, Other MS-CoNS: Other Methicillin-susceptible Coagulase-Negative Staphylococci, VSE : Vancomycin-sensitive Enterococcus faecalis , VRE: Vancomycin-resistant Enterococcus faecalis , C. simulans : Corynebacterium simulans , D.incerta: Desemzia incerta, N.dassonville: Nocardiopsis dassonville, C.avidum: Cutibacterium avidum, F.magna : Finegoldia magna , B . melitensis : Brucella melitensis , E.coli : Escherichia coli, P.mirabilis: Proteus mirabilis, E.tarda: Edwardsiella tarda, P.aeruginosa: Pseudomonas aeruginosa, P.stutzeri: Pseudomonas stutzeri, P.oryzihabitans: Pseudomonas oryzihabitans, L.adecarboxylata: Leclercia adecarboxylata

    Journal: BMC Microbiology

    Article Title: Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections

    doi: 10.1186/s12866-021-02164-8

    Figure Lengend Snippet: Distribution of detected microorganisms from PJIs MRSA: Methicillin-resistant Staphylococcus aureus, MSSA: Methicillin-susceptible Staphylococcus aureus , MRSE: Methicillin-resistant Staphylococcus epidermidis , MSSE: Methicillin-susceptible Staphylococcus epidermidis , MR- S. haemolyticus: Methicillin-resistant Staphylococcus haemolyticus , Other MR-CoNS: Other Methicillin-Resistant Coagulase-Negative Staphylococci, Other MS-CoNS: Other Methicillin-susceptible Coagulase-Negative Staphylococci, VSE : Vancomycin-sensitive Enterococcus faecalis , VRE: Vancomycin-resistant Enterococcus faecalis , C. simulans : Corynebacterium simulans , D.incerta: Desemzia incerta, N.dassonville: Nocardiopsis dassonville, C.avidum: Cutibacterium avidum, F.magna : Finegoldia magna , B . melitensis : Brucella melitensis , E.coli : Escherichia coli, P.mirabilis: Proteus mirabilis, E.tarda: Edwardsiella tarda, P.aeruginosa: Pseudomonas aeruginosa, P.stutzeri: Pseudomonas stutzeri, P.oryzihabitans: Pseudomonas oryzihabitans, L.adecarboxylata: Leclercia adecarboxylata

    Article Snippet: Reference strains used in this study were S. aureus ATCC 29213, S. aureus ATCC 25923, S. epidermidis strain Bjg (MK 516263), S. hemolyticus ATCC 29970, S. saprophyticus ATCC 15305, Micrococcus luteus ATCC 15307, Enterococcus faecalis ATCC 29212, Streptococcus sanguinis ATCC 10566, Finegoldia magna ATCC 1766, Cutibacterium ATCC 25573, Haemophilus influenza ATCC 49766, Pseudomonas aeruginosa 27,853, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 700603, Proteus mirabilis ATC 43071, Acinetobacter baumannii ATCC 19606, Brucella melitensis 16 M ATCC 23456, Mycobacterium tuberculosis H37Rv, Corynebacterium simulans strain BJd (MK 516260).

    Techniques:

    Detection of low bacterial concentrations in vegetative forms. Raman spectra from 10 spiked samples (SS) and 10 negative controls (NC) were collected and analyzed using a three-component partial least squared with discriminant analysis (PLS-DA) model. Classification and score plots obtained in components number 1 and 2 of (A-B) SS with 50 CFU/ml of S. enterica (SS-50-SE); (C-D) SS with 10 CFU/ml of S. enterica (SS-10-SE); (E-F) SS with 50 CFU/ml of S. haemolyticus (SS-50-SH); (G-H) SS with 10 CFU/ml of S. haemolyticus (SS-10-SH).

    Journal: bioRxiv

    Article Title: Detection of low numbers of bacterial cells in pharmaceutical drug product using Raman Spectroscopy and PLS-DA multivariate analysis

    doi: 10.1101/2022.04.26.489535

    Figure Lengend Snippet: Detection of low bacterial concentrations in vegetative forms. Raman spectra from 10 spiked samples (SS) and 10 negative controls (NC) were collected and analyzed using a three-component partial least squared with discriminant analysis (PLS-DA) model. Classification and score plots obtained in components number 1 and 2 of (A-B) SS with 50 CFU/ml of S. enterica (SS-50-SE); (C-D) SS with 10 CFU/ml of S. enterica (SS-10-SE); (E-F) SS with 50 CFU/ml of S. haemolyticus (SS-50-SH); (G-H) SS with 10 CFU/ml of S. haemolyticus (SS-10-SH).

    Article Snippet: S. haemolyticus (ATCC 29970) was kindly provided from Microbial Competence Centre, Novo Nordisk.

    Techniques: