haemolyticus atcc 29970 t  (ATCC)


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    Structured Review

    ATCC haemolyticus atcc 29970 t
    Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. <t>haemolyticus</t> , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​
    Haemolyticus Atcc 29970 T, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/haemolyticus atcc 29970 t/product/ATCC
    Average 94 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    haemolyticus atcc 29970 t - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "Programmable molecular circuit discriminates multidrug-resistant bacteria"

    Article Title: Programmable molecular circuit discriminates multidrug-resistant bacteria

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2022.100379

    Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. haemolyticus , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​
    Figure Legend Snippet: Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. haemolyticus , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​

    Techniques Used: Fluorescence, Concentration Assay

    2) Product Images from "Detection of low numbers of bacterial cells in pharmaceutical drug product using Raman Spectroscopy and PLS-DA multivariate analysis"

    Article Title: Detection of low numbers of bacterial cells in pharmaceutical drug product using Raman Spectroscopy and PLS-DA multivariate analysis

    Journal: bioRxiv

    doi: 10.1101/2022.04.26.489535

    Detection of low bacterial concentrations in vegetative forms. Raman spectra from 10 spiked samples (SS) and 10 negative controls (NC) were collected and analyzed using a three-component partial least squared with discriminant analysis (PLS-DA) model. Classification and score plots obtained in components number 1 and 2 of (A-B) SS with 50 CFU/ml of S. enterica (SS-50-SE); (C-D) SS with 10 CFU/ml of S. enterica (SS-10-SE); (E-F) SS with 50 CFU/ml of S. haemolyticus (SS-50-SH); (G-H) SS with 10 CFU/ml of S. haemolyticus (SS-10-SH).
    Figure Legend Snippet: Detection of low bacterial concentrations in vegetative forms. Raman spectra from 10 spiked samples (SS) and 10 negative controls (NC) were collected and analyzed using a three-component partial least squared with discriminant analysis (PLS-DA) model. Classification and score plots obtained in components number 1 and 2 of (A-B) SS with 50 CFU/ml of S. enterica (SS-50-SE); (C-D) SS with 10 CFU/ml of S. enterica (SS-10-SE); (E-F) SS with 50 CFU/ml of S. haemolyticus (SS-50-SH); (G-H) SS with 10 CFU/ml of S. haemolyticus (SS-10-SH).

    Techniques Used:

    3) Product Images from "Global Expansion of Linezolid-Resistant Coagulase-Negative Staphylococci"

    Article Title: Global Expansion of Linezolid-Resistant Coagulase-Negative Staphylococci

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2021.661798

    Maximum-likelihood phylogeny of S. haemolyticus population ( n = 207) based on core-SNP alignment. Background color fill is matched to BAPS clustering (BAPS 1 to BAPS 4). Linezolid-restant isolates (LRS reports) isolates is marked triangles: from Russia (one isolate in current study) and the United States ( Tewhey et al., 2014 ). Annotation from inner to outer circle: presence mecA ; MLST data; outer bar chart is matched to the number of acquired resistance genes (from 0 to 13 genes, specific chromosomal mutations were not included). The following genes were screened: aac(6′)-aph(2″), aadD, ant(6)-Ia, ant(9)-Ia, aph(3′)-III, blaZ, cat, dfrG, ermA, ermC, cfr, fexA, fosB, fosD, fusB, fusC, lnu(A), lsa(B), mecA, mph(C), msr(A), str, tetC, tetK, tetL, tetM, vgaA, and vgaB.
    Figure Legend Snippet: Maximum-likelihood phylogeny of S. haemolyticus population ( n = 207) based on core-SNP alignment. Background color fill is matched to BAPS clustering (BAPS 1 to BAPS 4). Linezolid-restant isolates (LRS reports) isolates is marked triangles: from Russia (one isolate in current study) and the United States ( Tewhey et al., 2014 ). Annotation from inner to outer circle: presence mecA ; MLST data; outer bar chart is matched to the number of acquired resistance genes (from 0 to 13 genes, specific chromosomal mutations were not included). The following genes were screened: aac(6′)-aph(2″), aadD, ant(6)-Ia, ant(9)-Ia, aph(3′)-III, blaZ, cat, dfrG, ermA, ermC, cfr, fexA, fosB, fosD, fusB, fusC, lnu(A), lsa(B), mecA, mph(C), msr(A), str, tetC, tetK, tetL, tetM, vgaA, and vgaB.

    Techniques Used:

    4) Product Images from "Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide"

    Article Title: Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide

    Journal: Antibiotics

    doi: 10.3390/antibiotics10070879

    ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. haemolyticus A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.
    Figure Legend Snippet: ( a , b ): SEM results for the same S. saprophyticus isolate: ( a ): pure culture; ( b ): S. saprophyticus isolate incubated with 200 µg/mL RIP. ( c , d ): S. epidermidis ADBC carrying the complete operon; ( c ): pure culture; ( d ): S. epidermidis incubated with 200 µg/mL RIP. ( e , f ): S. epidermidis DBC carrying the icaDBC genes; ( e ): pure culture; ( f ): S. epidermidis incubated with 200 µg/mL RIP. ( g , h ): S. haemolyticus A expressing only the icaA gene; ( g ): pure culture; ( h ): S. haemolyticus incubated with 200 µg/mL RIP. ( i , j ): SEM results for the same S. hominis isolate; ( i ): pure culture; ( j ): S. hominis incubated with 200 µg/mL RIP. ( k , l ): SEM results for the same S. warneri isolate; ( k ): pure culture; ( l ): S. warneri incubated with 200 µg/mL RIP. ( m , n ): SEM results for S. lugdunensis : ( m ): pure culture; ( n ): S. lugdunensis incubated with 200 µg/mL RIP. ( o , p ): SEM results for the same S. aureus isolate: ( o ): pure culture; ( p ): S. aureus incubated with 200 µg/mL RIP. Scale bars: ( a , b ): 10 µm; ( c , d , k – p ): 20 µm; ( e – h ): 10 µm; ( i , j ): 5 µm.

    Techniques Used: Incubation, Expressing

    5) Product Images from "Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland"

    Article Title: Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland

    Journal: Pathogens

    doi: 10.3390/pathogens10070877

    Agarose gel electrophoresis of multiplex PCR performed in order to identify bacterial species and detect the presence of the mecA gene: M—DNA molecular weight marker 100 bp (GeneRuler 100 bp DNA Ladder, ThermoFisher Scientific), 1- . aureus ATCC 25923 mecA (–) strain, 2- S. aureus ATCC 43300 mecA (+) strain, 3-clinical mecA (+) strain, 4- clinical Staphylococcus epidermidis strain, 5- Staphylococcus haemolyticus ATCC 29970 strain, 6– Staphylococcus epidermidis ATCC 12228 strain, 7-16-clinical Staphylococcus epidermidis strains. List of expected amplicons for (1) species identification: S. aureus -108 bp; S. epidermidis -124 bp; S. haemolyticus -271 bp; (2) mecA gene-154 bp.
    Figure Legend Snippet: Agarose gel electrophoresis of multiplex PCR performed in order to identify bacterial species and detect the presence of the mecA gene: M—DNA molecular weight marker 100 bp (GeneRuler 100 bp DNA Ladder, ThermoFisher Scientific), 1- . aureus ATCC 25923 mecA (–) strain, 2- S. aureus ATCC 43300 mecA (+) strain, 3-clinical mecA (+) strain, 4- clinical Staphylococcus epidermidis strain, 5- Staphylococcus haemolyticus ATCC 29970 strain, 6– Staphylococcus epidermidis ATCC 12228 strain, 7-16-clinical Staphylococcus epidermidis strains. List of expected amplicons for (1) species identification: S. aureus -108 bp; S. epidermidis -124 bp; S. haemolyticus -271 bp; (2) mecA gene-154 bp.

    Techniques Used: Agarose Gel Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Molecular Weight, Marker

    6) Product Images from "Coagulase-Negative Staphylococci Clones Are Widely Distributed in the Hospital and Community"

    Article Title: Coagulase-Negative Staphylococci Clones Are Widely Distributed in the Hospital and Community

    Journal: Pathogens

    doi: 10.3390/pathogens10070792

    Dendrogram generated by Dice/UPGMA analysis (Bionumerics Applied Maths), from PFGE- SmaI profiles of S. haemolyticus isolated from nasal swabs ( A ), wounds ( B ), and blood cultures ( C ). Square brackets highlight the clusters ( > 80% similarity). Roman numerals represent the SCC mec type.
    Figure Legend Snippet: Dendrogram generated by Dice/UPGMA analysis (Bionumerics Applied Maths), from PFGE- SmaI profiles of S. haemolyticus isolated from nasal swabs ( A ), wounds ( B ), and blood cultures ( C ). Square brackets highlight the clusters ( > 80% similarity). Roman numerals represent the SCC mec type.

    Techniques Used: Generated, Isolation

    7) Product Images from "Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections"

    Article Title: Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections

    Journal: BMC Microbiology

    doi: 10.1186/s12866-021-02164-8

    A: HRMA Derivative Melting Curves and B: HRMA Aligned Melting Curves for some of Reference strains 1a: V1 region of K. pneumonia ATCC 700603, 1b: V3 region of K. pneumonia ATCC 700603, 1c: V6 region of K. pneumonia ATCC 700603, 2a: V1 region of S. aureus ATCC 25923 , 2b: V3 region of S. aureus ATCC 25923, 2c: V6 region of S. aureus ATCC 25923, 3a: V1 region of B. melitensis 16 M ATCC 23456, 3b: V3 region of B. melitensis 16 M ATCC 23456, 3c: V6 region of B. melitensis 16 M ATCC 23456
    Figure Legend Snippet: A: HRMA Derivative Melting Curves and B: HRMA Aligned Melting Curves for some of Reference strains 1a: V1 region of K. pneumonia ATCC 700603, 1b: V3 region of K. pneumonia ATCC 700603, 1c: V6 region of K. pneumonia ATCC 700603, 2a: V1 region of S. aureus ATCC 25923 , 2b: V3 region of S. aureus ATCC 25923, 2c: V6 region of S. aureus ATCC 25923, 3a: V1 region of B. melitensis 16 M ATCC 23456, 3b: V3 region of B. melitensis 16 M ATCC 23456, 3c: V6 region of B. melitensis 16 M ATCC 23456

    Techniques Used:

    HRMA Derivative Melting Curves of Reference strains for V1, V3, V6 regions of S. aureus ATCC 25923 and S. epidermidis strain Bjg (MK 516263) 1a: V1 region of S. aureus , 1b: V1 region of S. epidermidis , 2a: V6 region of S. aureus , 2b: V6 region of S. epidermidis, 3a: V3 region of S. aureus , 3b: V3 region of S. epidermidis
    Figure Legend Snippet: HRMA Derivative Melting Curves of Reference strains for V1, V3, V6 regions of S. aureus ATCC 25923 and S. epidermidis strain Bjg (MK 516263) 1a: V1 region of S. aureus , 1b: V1 region of S. epidermidis , 2a: V6 region of S. aureus , 2b: V6 region of S. epidermidis, 3a: V3 region of S. aureus , 3b: V3 region of S. epidermidis

    Techniques Used:

    a : HRMA Derivative Melting Curves and b : HRMA Aligned Melting Curves for V1, V3, V6 regions of S. aureus ATCC 25923 and S. aureus isolated from clinical (PJI) specimens Ref-V1: Reference strain-V1 region, S-V1: Clinical specimen-V1 region, Ref-V3: Reference strain-V3 region, S-V3: Clinical specimen-V3 region, Ref-V6: Reference strain-V6 region, S-V6: Clinical specimen- V6 region
    Figure Legend Snippet: a : HRMA Derivative Melting Curves and b : HRMA Aligned Melting Curves for V1, V3, V6 regions of S. aureus ATCC 25923 and S. aureus isolated from clinical (PJI) specimens Ref-V1: Reference strain-V1 region, S-V1: Clinical specimen-V1 region, Ref-V3: Reference strain-V3 region, S-V3: Clinical specimen-V3 region, Ref-V6: Reference strain-V6 region, S-V6: Clinical specimen- V6 region

    Techniques Used: Isolation

    Distribution of detected microorganisms from PJIs MRSA: Methicillin-resistant Staphylococcus aureus, MSSA: Methicillin-susceptible Staphylococcus aureus , MRSE: Methicillin-resistant Staphylococcus epidermidis , MSSE: Methicillin-susceptible Staphylococcus epidermidis , MR- S. haemolyticus: Methicillin-resistant Staphylococcus haemolyticus , Other MR-CoNS: Other Methicillin-Resistant Coagulase-Negative Staphylococci, Other MS-CoNS: Other Methicillin-susceptible Coagulase-Negative Staphylococci, VSE : Vancomycin-sensitive Enterococcus faecalis , VRE: Vancomycin-resistant Enterococcus faecalis , C. simulans : Corynebacterium simulans , D.incerta: Desemzia incerta, N.dassonville: Nocardiopsis dassonville, C.avidum: Cutibacterium avidum, F.magna : Finegoldia magna , B . melitensis : Brucella melitensis , E.coli : Escherichia coli, P.mirabilis: Proteus mirabilis, E.tarda: Edwardsiella tarda, P.aeruginosa: Pseudomonas aeruginosa, P.stutzeri: Pseudomonas stutzeri, P.oryzihabitans: Pseudomonas oryzihabitans, L.adecarboxylata: Leclercia adecarboxylata
    Figure Legend Snippet: Distribution of detected microorganisms from PJIs MRSA: Methicillin-resistant Staphylococcus aureus, MSSA: Methicillin-susceptible Staphylococcus aureus , MRSE: Methicillin-resistant Staphylococcus epidermidis , MSSE: Methicillin-susceptible Staphylococcus epidermidis , MR- S. haemolyticus: Methicillin-resistant Staphylococcus haemolyticus , Other MR-CoNS: Other Methicillin-Resistant Coagulase-Negative Staphylococci, Other MS-CoNS: Other Methicillin-susceptible Coagulase-Negative Staphylococci, VSE : Vancomycin-sensitive Enterococcus faecalis , VRE: Vancomycin-resistant Enterococcus faecalis , C. simulans : Corynebacterium simulans , D.incerta: Desemzia incerta, N.dassonville: Nocardiopsis dassonville, C.avidum: Cutibacterium avidum, F.magna : Finegoldia magna , B . melitensis : Brucella melitensis , E.coli : Escherichia coli, P.mirabilis: Proteus mirabilis, E.tarda: Edwardsiella tarda, P.aeruginosa: Pseudomonas aeruginosa, P.stutzeri: Pseudomonas stutzeri, P.oryzihabitans: Pseudomonas oryzihabitans, L.adecarboxylata: Leclercia adecarboxylata

    Techniques Used:

    8) Product Images from "Comparative characterisation of human and ovine non-aureus staphylococci isolated in Sardinia (Italy) for antimicrobial susceptibility profiles and resistance genes"

    Article Title: Comparative characterisation of human and ovine non-aureus staphylococci isolated in Sardinia (Italy) for antimicrobial susceptibility profiles and resistance genes

    Journal: Epidemiology and Infection

    doi: 10.1017/S0950268821000212

    RFLP patterns obtained for 15 reference strains after digestion of the gap gene amplicon with Alu I. Fragments were separated by 12% PAGE gel. Lane M, marker VIII (Roche); lane 1, S. xylosus ATCC 29971; lane 2, S. saprophyticus ATCC 15305; lane 3, S. capitis ATCC 27840; lane 4, S. haemolyticus ATCC 29970; lane 5, S. simulans ATCC 27848, lane 6, S. warneri ATCC 27836; lane 7, S. arlettae ATCC 43957; lane 8, S. chromogenes ATCC 43764; lane 9, S. equorum ATCC 43958; lane 10, S. hominis ATCC 27844; lane 11, S. caprae ATCC 35538; lane 12, S. lentus ATCC 29070; lane 13, S. hyicus ATCC 11249, lane 14, S. epidermidis ATCC 14990 and lane 14, S. aureus ATCC 43300.
    Figure Legend Snippet: RFLP patterns obtained for 15 reference strains after digestion of the gap gene amplicon with Alu I. Fragments were separated by 12% PAGE gel. Lane M, marker VIII (Roche); lane 1, S. xylosus ATCC 29971; lane 2, S. saprophyticus ATCC 15305; lane 3, S. capitis ATCC 27840; lane 4, S. haemolyticus ATCC 29970; lane 5, S. simulans ATCC 27848, lane 6, S. warneri ATCC 27836; lane 7, S. arlettae ATCC 43957; lane 8, S. chromogenes ATCC 43764; lane 9, S. equorum ATCC 43958; lane 10, S. hominis ATCC 27844; lane 11, S. caprae ATCC 35538; lane 12, S. lentus ATCC 29070; lane 13, S. hyicus ATCC 11249, lane 14, S. epidermidis ATCC 14990 and lane 14, S. aureus ATCC 43300.

    Techniques Used: Amplification, Polyacrylamide Gel Electrophoresis, Marker

    9) Product Images from "Pathogenesis of Staphylococcus haemolyticus on primary human skin fibroblast cells"

    Article Title: Pathogenesis of Staphylococcus haemolyticus on primary human skin fibroblast cells

    Journal: Virulence

    doi: 10.1080/21505594.2020.1809962

    Bacterial adhesion to PHSF cells as detected by Giemsa stain. After 3 h in culture, PHSF cells were challenged with different S. haemolyticus isolates. (a) Non-infected fibroblast cells, (b) Fibroblasts infected with S. haemolyticus isolate with low adhesion capacity, (c) Fibroblasts infected with S. haemolyticus isolate with high adhesion capacity, (d) Fibroblasts infected with the control S. haemolyticus strain (ATCC 29970) showing low adhesion capacity.
    Figure Legend Snippet: Bacterial adhesion to PHSF cells as detected by Giemsa stain. After 3 h in culture, PHSF cells were challenged with different S. haemolyticus isolates. (a) Non-infected fibroblast cells, (b) Fibroblasts infected with S. haemolyticus isolate with low adhesion capacity, (c) Fibroblasts infected with S. haemolyticus isolate with high adhesion capacity, (d) Fibroblasts infected with the control S. haemolyticus strain (ATCC 29970) showing low adhesion capacity.

    Techniques Used: Giemsa Stain, Infection

    Invasion of PHSF cells by FITC-labeled bacteria. Comparative histograms represent results of flow cytometry analysis of (a) control non-infected cells (mock), (b) poorly invasive strain of S. haemolyticus (SH1), (c) highly invasive strain of S. haemolyticus (SH10), (d) Control S. haemolyticus strain (ATCC 29970). The figure shows a representative of three independent experiments.
    Figure Legend Snippet: Invasion of PHSF cells by FITC-labeled bacteria. Comparative histograms represent results of flow cytometry analysis of (a) control non-infected cells (mock), (b) poorly invasive strain of S. haemolyticus (SH1), (c) highly invasive strain of S. haemolyticus (SH10), (d) Control S. haemolyticus strain (ATCC 29970). The figure shows a representative of three independent experiments.

    Techniques Used: Labeling, Flow Cytometry, Infection

    Flow cytometry analysis showing the cytotoxic effect of SH and SA on the PHSF cells. Cells were treated with indicated microbial products and stained after 24 h of incubation with Annexin V and PI. (a) Cells were infected with SH (upper panel), while in (b) cells were infected with SA (lower panel). (c) The mean of the cytotoxic activities of different tested strains on the PHSF cells ± SD. SH, S. haemolyticus ; SA, S. aureus.
    Figure Legend Snippet: Flow cytometry analysis showing the cytotoxic effect of SH and SA on the PHSF cells. Cells were treated with indicated microbial products and stained after 24 h of incubation with Annexin V and PI. (a) Cells were infected with SH (upper panel), while in (b) cells were infected with SA (lower panel). (c) The mean of the cytotoxic activities of different tested strains on the PHSF cells ± SD. SH, S. haemolyticus ; SA, S. aureus.

    Techniques Used: Flow Cytometry, Staining, Incubation, Infection

    Hemolytic activities ofclinical S. haemolyticus isolates compared to S. aureus and control S. haemolyticus (ATCC 29970) after 5 h of incubation. SH; S. haemolyticus , SA; S. aureus . Depicted are the mean of 3 independent experiments ± standard deviation.
    Figure Legend Snippet: Hemolytic activities ofclinical S. haemolyticus isolates compared to S. aureus and control S. haemolyticus (ATCC 29970) after 5 h of incubation. SH; S. haemolyticus , SA; S. aureus . Depicted are the mean of 3 independent experiments ± standard deviation.

    Techniques Used: Incubation, Standard Deviation

    The PHSF cells were challenged with S. haemolyticus at an MOI of 10 and examined with TEM. (a) After 15 min of infection, extracellular bacteria were observed (3600x) which were further magnified (14,000 x) in (b). (c) After 1 h of infection, bacteria invaded the cells and became engulfed (3600 x). (d) Initialization of phagocytosis. (e) After 90 min, S. haemolyticus entered the PHSF cells and become localized in vacuoles (3600 x). (f) Proliferating bacteria are shown inside the vacuole.
    Figure Legend Snippet: The PHSF cells were challenged with S. haemolyticus at an MOI of 10 and examined with TEM. (a) After 15 min of infection, extracellular bacteria were observed (3600x) which were further magnified (14,000 x) in (b). (c) After 1 h of infection, bacteria invaded the cells and became engulfed (3600 x). (d) Initialization of phagocytosis. (e) After 90 min, S. haemolyticus entered the PHSF cells and become localized in vacuoles (3600 x). (f) Proliferating bacteria are shown inside the vacuole.

    Techniques Used: Transmission Electron Microscopy, Infection

    Cytokine expression profile of PBMCs incubated with S. haemolyticus and S. aureus for 6 and 24 h . The figure shows the mean of 3 independent experiments ± standard error.
    Figure Legend Snippet: Cytokine expression profile of PBMCs incubated with S. haemolyticus and S. aureus for 6 and 24 h . The figure shows the mean of 3 independent experiments ± standard error.

    Techniques Used: Expressing, Incubation

    Percentage of internalized S. haemolyticus in the PHSF cells and the distribution of the fnBP genes.
    Figure Legend Snippet: Percentage of internalized S. haemolyticus in the PHSF cells and the distribution of the fnBP genes.

    Techniques Used:

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    ATCC haemolyticus atcc 29970 t
    Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. <t>haemolyticus</t> , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​
    Haemolyticus Atcc 29970 T, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/haemolyticus atcc 29970 t/product/ATCC
    Average 94 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    haemolyticus atcc 29970 t - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

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    Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. haemolyticus , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​

    Journal: Materials Today Bio

    Article Title: Programmable molecular circuit discriminates multidrug-resistant bacteria

    doi: 10.1016/j.mtbio.2022.100379

    Figure Lengend Snippet: Performance of the BRICK strategy. (A) Fluorescence spectra after adding the following series of concentrations of MRSA (0, 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 ​CFU/mL, respectively). (B) Linear correlation between the fluorescence peak and the logarithm concentration of MRSA. Concentration of MoS 2 nanosheets: 5 ​μg/mL; cy5 probe: 100 ​nM. (C) Responses of fluorescence peak for MRSA, S. aureus , S. haemolyticus , S. pneumoniae , S. pyogenes , S. mitis , and M. luteus , respectively. The concentration of each bacterium is 10 6 ​CFU/mL, ∗∗∗∗ p ​

    Article Snippet: Methicillin-resistant Staphylococcus aureus ATCC 43300 (MRSA), Staphylococcus aureus ATCC 29213 (S. aureus ), Staphylococcus haemolyticus ATCC 29970 (S. haemolyticus ), Streptococcus pneumoniae ATCC 49619 (S. pneumoniae ), Streptococcus pyogenes ATCC 19615 (S. pyogenes ), Streptococcus mitis ATCC 49456 (S. mitis ), Micrococcus luteus ATCC 4698 (M. luteus ), and the clinical strains of MRSA, MDR Acinetobacter baumannii (A. baumannii ), Mycobacterium tuberculosis (M. tuberculosis ), Pseudomonas aeruginosa (P. aeruginosa ), and Escherichia coli (E. coli) were obtained from Chongqing University Cancer Hospital.

    Techniques: Fluorescence, Concentration Assay