Journal: PLoS ONE
Article Title: Cathepsin K Is Present in Invasive Oral Tongue Squamous Cell Carcinoma In Vivo and In Vitro
doi: 10.1371/journal.pone.0070925
Figure Lengend Snippet: The knockdown of cathepsin K mRNA has significantly decreased the invasion of HSC-3 shRNA CTSK cells in the myoma organotypic model when compared with HSC-3 shRNA control cells (A–B). HSC-3 shRNA CTSK cells showed a reduction in cathepsin K mRNA levels when compared with wild type cells and HSC-3 cells transfected with a control shRNA (scramble) *p<0.0001 (C). The invasion depth, invasion area, and invasion index were all significantly reduced for HSC-3-shRNA CTSK cells (*p = 0.0006, **p<0.0001, and ***p = 0.0008, respectively) (D–F). Scale bars 200 µm.
Article Snippet: CTSK shRNA and control shRNA lentiviral particles (reference numbers sc-29936-V and sc-108080) were purchased from Santa Cruz Biotechnology (USA).
Techniques: Knockdown, shRNA, Control, Transfection