Journal: Scientific Reports
Article Title: MALDI-TOF-MS based identification and molecular characterization of food associated methicillin-resistant Staphylococcus aureus
Figure Lengend Snippet: ( A ) MDR Staphylococcal strains discriminated by universally conserved thermonuclease ( nuc ) gene. Isolated 160 Staphylococcal spp., were screened to identify at species level by PCR using universally conserved thermonuclease ( nuc ) gene. PCR confirmed as 151 methicillin resistant S . aureus (Lane 1–4, 359 bp), 6 as S . epidermidis (Lane 5–8, 251 bp) and 2 as S . haemolyticus (Lane 9 and 10, 434 bp) used along with E . coli (Lane 11), S . typhimurium (Lane 12), S . flexneri (Lane 13), V . cholera (Lane 14), and V . parahaemolyticus (Lane 15) were compared with 100 bp ladder (M) and water as negative control (C-). ( B) Illustration of PCR amplicons expected when using the updated Staphylococcal spp. multiplex PCR. Lane M, 100 bp ladder; Lane 1, negative control (C-); Lane 2, mixture of multiplex PCR products obtained for all the templates of MDR-MRSA, MRSE, MRSH and S . typhimurium; Lane 3, MRSA; Lane 4, MRSE ; Lane 5, MRSH and Lane 6, S . typhimurium comparable to the amplified product of mPCR in lane 2.
Article Snippet: Salmonella typhimurium (98), Escherichia coli (1610), Staphylococcus aureus (96), Bacillus cereus (430), Shigella flexneri (1457), Vibrio cholera (3904), Vibrio paraheamolyticus (451), Pseudomonas aeruginosa (1688) and Enterobacter aerogenes (13048) strains were cultured as per the protocol prescribed by MTCC and ATCC.
Techniques: Isolation, Polymerase Chain Reaction, Negative Control, Multiplex Assay, Amplification