Journal: Microbiology Spectrum
Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection
doi: 10.1128/spectrum.02906-24
Figure Lengend Snippet: Specificity of LAMP assay for the detection of M. marinum . HNB-LAMP reaction solution indicates positive (blue) and negative (purple). Each amplified product was analyzed by agarose gel electrophoresis. Lanes (tubes) are labeled as follows: lane M: 100 bp ladder marker; lane 1: Mycobacterium abscessus JCM 13569; lane 2: Mycobacterium avium 104; lane 3: M. chelonae JCM 6388; lane 4: M. fortuitum clinical isolate; lane 5: M. haemophilum ATCC 29548; lane 6: M. marinum ATCC 927; lane 7: M. marinum clinical isolate LRC-1; lane 8: M. marinum clinical isolate LRC-9; lane 9: M. marinum clinical isolate LRC-16; lane 10: M. pseudoshottsii JCM 15466; lane M: DNA size marker (100 bp ladder); lane 11: Mycobacterium smegmatis mc 2 155; lane 12: M. ulcerans Agy99; lane 13: M. ulcerans subsp. shinshuense ATCC 33728; and lane 14: negative control (no DNA).
Article Snippet: M. haemophilum , ATCC 29548 , 1 , 0.
Techniques: Lamp Assay, Amplification, Agarose Gel Electrophoresis, Labeling, Marker, Negative Control