293fectin lipid transfection  (Thermo Fisher)


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    Name:
    293fectin Transfection Reagent
    Description:
    293fectin Transfection Reagent is a proprietary cationic lipid based formulation for transfecting DNA into eukaryotic cells This reagent is optimized for transfecting suspension 293 human embryonic kidney cells e g FreeStyle 293 F cells Cat No R790 07 in defined serum free FreeStyle 293 Expression Medium Cat No 12338 018 and is intended for use with the FreeStyle 293 Expression System Cat No K9000 01 293fectin Transfection Reagent provides the following advantages • Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells• Suspension FreeStyle 293 F cells may be transfected in FreeStyle 293 Expression Medium no medium change is required• Add 293fectin reagent complexes directly to cells in culture medium• No need to remove complexes or change or add medium following transfection
    Catalog Number:
    12347019
    Price:
    None
    Applications:
    Bioproduction|Cell Culture|Mammalian Expression|Mammalian Protein Production|Plasmid Transfection|Protein Biology|Protein Expression|Transient Protein Production & High-Throughput Screening|Antibody & Cell Culture Production|Transfection
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher 293fectin lipid transfection
    293fectin Transfection Reagent is a proprietary cationic lipid based formulation for transfecting DNA into eukaryotic cells This reagent is optimized for transfecting suspension 293 human embryonic kidney cells e g FreeStyle 293 F cells Cat No R790 07 in defined serum free FreeStyle 293 Expression Medium Cat No 12338 018 and is intended for use with the FreeStyle 293 Expression System Cat No K9000 01 293fectin Transfection Reagent provides the following advantages • Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells• Suspension FreeStyle 293 F cells may be transfected in FreeStyle 293 Expression Medium no medium change is required• Add 293fectin reagent complexes directly to cells in culture medium• No need to remove complexes or change or add medium following transfection
    https://www.bioz.com/result/293fectin lipid transfection/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    293fectin lipid transfection - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    antibody expression antibodies
    hek-293 cells

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    Related Articles

    Clone Assay:

    Article Title: Disposal of iron by a mutant form of lipocalin 2
    Article Snippet: .. To produce LCN2 in mammalian cells, human LCN2 ORF was cloned to pcDNA3.1(+) to generate pcDNA3.1(+)-LCN2 which was then transfected into Freestyle 293-F cells using 293fectin (Life Technologies). .. Stably transfected cell lines were selected using G418 (800 μg ml−1 ) and the culture medium was collected and concentrated for purification by using LPS-free column chromatography ( ).

    Transfection:

    Article Title: Exploring the Dynamic Range of the Kinetic Exclusion Assay in Characterizing Antigen-Antibody Interactions
    Article Snippet: .. To obtain conditioned media containing human DKK2, cyno DKK2 or rhesus DKK4, 293F cells were grown in defined serum-free media (Freestyle Expression Media, Invitrogen) and transiently transfected with an expression construct for the respective antigen using 293Fectin (Invitrogen) according to the manufacturer's protocol. .. Twenty four hours after transfection, heparin (Sigma-Aldrich, St. Louis, MO) was added at 0.1 g/L to enhance recovery of DKK in the conditioned media .

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

    Article Title: Disposal of iron by a mutant form of lipocalin 2
    Article Snippet: .. To produce LCN2 in mammalian cells, human LCN2 ORF was cloned to pcDNA3.1(+) to generate pcDNA3.1(+)-LCN2 which was then transfected into Freestyle 293-F cells using 293fectin (Life Technologies). .. Stably transfected cell lines were selected using G418 (800 μg ml−1 ) and the culture medium was collected and concentrated for purification by using LPS-free column chromatography ( ).

    Article Title: PROTEIN THERAPEUTICS FOR JUNCTIONAL EPIDERMOLYSIS BULLOSA: INCORPORATION OF RECOMBINANT ?3 CHAIN INTO LAMININ 332 IN ?3-/- KERATINOCYTES IN VITRO
    Article Snippet: .. Optimization of DNA transfection into 293F cells was carried out using 293Fectin™ transfection reagent (Invitrogen, Carlsbad, CA) and Amaxa nucleofection reaction (Amaxa Inc, Köln, Germany). .. Two and 4 μg of the pMaxGFP plasmid encoding green fluorescent protein (Amaxa Inc, Köln, Germany) were used for transfection and nucleofection, respectively.

    Article Title: Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies
    Article Snippet: .. The genes were transfected using 293fectin (Invitrogen) and harvested 4-5 days later. .. The cells were centrifuged at 4000rpm for 15min, filtered using 0.2 μm filter (Millipore) and a protease inhibitor was added at ratio of 1ml per liter of supernatant (Protease Arrest, GBiosciences).

    Article Title: PLAP efficiently generates mature antigenic peptides in vitro but in patterns distinct from ERAP1 1
    Article Snippet: .. Transfection was performed using the 293Fectin transfection reagent according to the freestyle293™ expression system (Invitrogen) instructions. .. Three days post tranfection the cell supernatant was harvested and expression confirmed by L-AMC assay and by western blotting using an anti-5xHis-HRP conjugate antibody (Qiagen).

    Article Title: Structural Insights into Neonatal Fc Receptor-based Recycling Mechanisms
    Article Snippet: .. HEK 293 cells were transiently transfected with the various HSA constructs using 293Fectin (Invitrogen) and standard protocols. ..

    Mutagenesis:

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

    Construct:

    Article Title: Exploring the Dynamic Range of the Kinetic Exclusion Assay in Characterizing Antigen-Antibody Interactions
    Article Snippet: .. To obtain conditioned media containing human DKK2, cyno DKK2 or rhesus DKK4, 293F cells were grown in defined serum-free media (Freestyle Expression Media, Invitrogen) and transiently transfected with an expression construct for the respective antigen using 293Fectin (Invitrogen) according to the manufacturer's protocol. .. Twenty four hours after transfection, heparin (Sigma-Aldrich, St. Louis, MO) was added at 0.1 g/L to enhance recovery of DKK in the conditioned media .

    Article Title: Structural Insights into Neonatal Fc Receptor-based Recycling Mechanisms
    Article Snippet: .. HEK 293 cells were transiently transfected with the various HSA constructs using 293Fectin (Invitrogen) and standard protocols. ..

    Expressing:

    Article Title: Exploring the Dynamic Range of the Kinetic Exclusion Assay in Characterizing Antigen-Antibody Interactions
    Article Snippet: .. To obtain conditioned media containing human DKK2, cyno DKK2 or rhesus DKK4, 293F cells were grown in defined serum-free media (Freestyle Expression Media, Invitrogen) and transiently transfected with an expression construct for the respective antigen using 293Fectin (Invitrogen) according to the manufacturer's protocol. .. Twenty four hours after transfection, heparin (Sigma-Aldrich, St. Louis, MO) was added at 0.1 g/L to enhance recovery of DKK in the conditioned media .

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

    Article Title: PLAP efficiently generates mature antigenic peptides in vitro but in patterns distinct from ERAP1 1
    Article Snippet: .. Transfection was performed using the 293Fectin transfection reagent according to the freestyle293™ expression system (Invitrogen) instructions. .. Three days post tranfection the cell supernatant was harvested and expression confirmed by L-AMC assay and by western blotting using an anti-5xHis-HRP conjugate antibody (Qiagen).

    Over Expression:

    Article Title: Pimelic Diphenylamide 106 Is a Slow, Tight-binding Inhibitor of Class I Histone Deacetylases *Pimelic Diphenylamide 106 Is a Slow, Tight-binding Inhibitor of Class I Histone Deacetylases * S⃞
    Article Snippet: .. Protein overexpression was achieved by transfecting plasmids into HEK293t cells with 293-fectin reagent (Invitrogen). .. Cells were lysed at 72 h with whole cell lysis buffer (50 m m Tris-HCl, pH 7.4, 150 m m NaCl, 0.5% Triton X-100, 10% glycerol), and passed over FLAG-M2 affinity resin (Sigma) for purification.

    Plasmid Preparation:

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

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  • 99
    Thermo Fisher freestyle 293 expression system
    Freestyle 293 Expression System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/freestyle 293 expression system/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    freestyle 293 expression system - by Bioz Stars, 2020-07
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    99
    Thermo Fisher 293fectin
    293fectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/293fectin/product/Thermo Fisher
    Average 99 stars, based on 277 article reviews
    Price from $9.99 to $1999.99
    293fectin - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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