blautia  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC blautia
    Blautia, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blautia/product/ATCC
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    blautia - by Bioz Stars, 2022-09
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    ATCC blautia coccoides
    Mucin-related glycosyl hydrolase profiles in the Firmicutes phlyum. ( A – C ) Heat map of the Firmicutes genomes that have at least one gene copy of mucin-associated GH 33, 16, 29, 95, 20, 2, 35, 42, 98, 101, 129, 89, 85, and 84. Heat map showing the gene copy number of mucin-associated GHs in the strains of ( D ) Abiotrophia defective , <t>Blautia</t> <t>coccoides,</t> B. hansenii, B. obeum, B. producta, Blautia spp., Enterococcus casseliflavus, E. durans, E. gallinarum , and Enterococcus spp., ( E ) Paenibacillus, specifically Paenibacillus spp., P barcinonensis and P. lautus, ( D ) Ruminococcus, including Ruminococcus spp. R. gnavus and R. torques, ( F ) Streptococcus, including S. australis (Sa), S. intermedius (Si), S. mitis (Sm), Streptococcus spp. and Viridibacillus spp. ( G ) Clostridium, including C. butyricum (Cb), C. sporogenes ( Cs), and Clostridium spp. ( H , I ) Growth analysis of Clostridium butyricum CB, Clostridium symbiosum ATCC 14940, Clostridium inoculum ATCC 14501, Clostridium clostridiforme ATCC 25532, and Clostridium sporogenes DSMZ 795 ( H ), as well as Lactobacillus gasseri ATCC 33323, L. johnsonii ATCC 33200, L. brevis ATCC 27305, L. acidophilus ATCC 4796 ( I ) in a chemically defined media ZMB1 lacking glucose (media control), with glucose (positive control), or lacking glucose and supplemented with 1 mg/mL porcine intestinal MUC2. Growth was measured by examining the optical density at 600 nm (OD 600nm ) after overnight incubation.
    Blautia Coccoides, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blautia coccoides/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    blautia coccoides - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Mucin-related glycosyl hydrolase profiles in the Firmicutes phlyum. ( A – C ) Heat map of the Firmicutes genomes that have at least one gene copy of mucin-associated GH 33, 16, 29, 95, 20, 2, 35, 42, 98, 101, 129, 89, 85, and 84. Heat map showing the gene copy number of mucin-associated GHs in the strains of ( D ) Abiotrophia defective , Blautia coccoides, B. hansenii, B. obeum, B. producta, Blautia spp., Enterococcus casseliflavus, E. durans, E. gallinarum , and Enterococcus spp., ( E ) Paenibacillus, specifically Paenibacillus spp., P barcinonensis and P. lautus, ( D ) Ruminococcus, including Ruminococcus spp. R. gnavus and R. torques, ( F ) Streptococcus, including S. australis (Sa), S. intermedius (Si), S. mitis (Sm), Streptococcus spp. and Viridibacillus spp. ( G ) Clostridium, including C. butyricum (Cb), C. sporogenes ( Cs), and Clostridium spp. ( H , I ) Growth analysis of Clostridium butyricum CB, Clostridium symbiosum ATCC 14940, Clostridium inoculum ATCC 14501, Clostridium clostridiforme ATCC 25532, and Clostridium sporogenes DSMZ 795 ( H ), as well as Lactobacillus gasseri ATCC 33323, L. johnsonii ATCC 33200, L. brevis ATCC 27305, L. acidophilus ATCC 4796 ( I ) in a chemically defined media ZMB1 lacking glucose (media control), with glucose (positive control), or lacking glucose and supplemented with 1 mg/mL porcine intestinal MUC2. Growth was measured by examining the optical density at 600 nm (OD 600nm ) after overnight incubation.

    Journal: Scientific Reports

    Article Title: Characterizing the mucin-degrading capacity of the human gut microbiota

    doi: 10.1038/s41598-022-11819-z

    Figure Lengend Snippet: Mucin-related glycosyl hydrolase profiles in the Firmicutes phlyum. ( A – C ) Heat map of the Firmicutes genomes that have at least one gene copy of mucin-associated GH 33, 16, 29, 95, 20, 2, 35, 42, 98, 101, 129, 89, 85, and 84. Heat map showing the gene copy number of mucin-associated GHs in the strains of ( D ) Abiotrophia defective , Blautia coccoides, B. hansenii, B. obeum, B. producta, Blautia spp., Enterococcus casseliflavus, E. durans, E. gallinarum , and Enterococcus spp., ( E ) Paenibacillus, specifically Paenibacillus spp., P barcinonensis and P. lautus, ( D ) Ruminococcus, including Ruminococcus spp. R. gnavus and R. torques, ( F ) Streptococcus, including S. australis (Sa), S. intermedius (Si), S. mitis (Sm), Streptococcus spp. and Viridibacillus spp. ( G ) Clostridium, including C. butyricum (Cb), C. sporogenes ( Cs), and Clostridium spp. ( H , I ) Growth analysis of Clostridium butyricum CB, Clostridium symbiosum ATCC 14940, Clostridium inoculum ATCC 14501, Clostridium clostridiforme ATCC 25532, and Clostridium sporogenes DSMZ 795 ( H ), as well as Lactobacillus gasseri ATCC 33323, L. johnsonii ATCC 33200, L. brevis ATCC 27305, L. acidophilus ATCC 4796 ( I ) in a chemically defined media ZMB1 lacking glucose (media control), with glucose (positive control), or lacking glucose and supplemented with 1 mg/mL porcine intestinal MUC2. Growth was measured by examining the optical density at 600 nm (OD 600nm ) after overnight incubation.

    Article Snippet: We also identified several commensal bacteria with mucin-associated GH profiles and GHs numbers (≥ 8 GH families) similar to Akkermansia , including Victavallales bacterium, Bifidobacterium bifidum, Streptomyces lividans, Blautia coccoides, B. hansenii, Bacteroides caccae, B. cellulosilyticus, B. dorei, B. fragilis, B. intestinalis, B. ovatus, B. thetaiotaomicron, B. uniformis, B. vulgatus, B. xylanisolvens, Fermentimonas caenicola, Parabacteroides distasonis, Phocaeicola coprophilus, P. dorei, P. vulgatus, and Paenibacillus lautus.

    Techniques: Positive Control, Incubation

    B. coccoides colonization restricts enteric virus systemic infection by activating innate cellular immune responses and type I IFN expression in a macrophage- and Ifnar -dependent manner. Frequency of mononuclear phagocytes expressing MHC-I (A) or CD80 (B) in PBMC or spleen isolated from PBS-treated mice, Abx-treated mice, or Abx-treated mice colonized with FMT, B. coccoides , or C. butyricum at 3 dpi following EMCV inoculation (representative flow cytometry histograms showing surface staining of MHC-I or CD80; n = 6). (C) Survival kinetics of infected Abx-treated and B. coccoides -colonized Abx-treated mice injected with clodronate liposomes ( n = 9 or 10). (D) Expression level of Ifnb in PBMCs isolated from infected PBS-treated mice, Abx-treated mice, or Abx-treated mice subjected to gavage with FMT, B. coccoides , or C. butyricum , compared to their respective mock-infected controls ( n = 5). (E) Survival kinetics of infected Abx-treated and B. coccoides -colonized Abx-treated Ifnar −/− mice ( n = 6 to 9). (F) Viral titers in blood and brain collected from infected PBS-treated, Abx-treated, or B. coccoides -colonized WT or Ifnar −/− mice at 1 dpi ( n = 5 or 6). All data are from two independent experiments, and P values were determined by unpaired two-tailed Student's t test. *, P

    Journal: mBio

    Article Title: The Intestinal Microbiome Primes Host Innate Immunity against Enteric Virus Systemic Infection through Type I Interferon

    doi: 10.1128/mBio.00366-21

    Figure Lengend Snippet: B. coccoides colonization restricts enteric virus systemic infection by activating innate cellular immune responses and type I IFN expression in a macrophage- and Ifnar -dependent manner. Frequency of mononuclear phagocytes expressing MHC-I (A) or CD80 (B) in PBMC or spleen isolated from PBS-treated mice, Abx-treated mice, or Abx-treated mice colonized with FMT, B. coccoides , or C. butyricum at 3 dpi following EMCV inoculation (representative flow cytometry histograms showing surface staining of MHC-I or CD80; n = 6). (C) Survival kinetics of infected Abx-treated and B. coccoides -colonized Abx-treated mice injected with clodronate liposomes ( n = 9 or 10). (D) Expression level of Ifnb in PBMCs isolated from infected PBS-treated mice, Abx-treated mice, or Abx-treated mice subjected to gavage with FMT, B. coccoides , or C. butyricum , compared to their respective mock-infected controls ( n = 5). (E) Survival kinetics of infected Abx-treated and B. coccoides -colonized Abx-treated Ifnar −/− mice ( n = 6 to 9). (F) Viral titers in blood and brain collected from infected PBS-treated, Abx-treated, or B. coccoides -colonized WT or Ifnar −/− mice at 1 dpi ( n = 5 or 6). All data are from two independent experiments, and P values were determined by unpaired two-tailed Student's t test. *, P

    Article Snippet: B. coccoides was purchased from ATCC (ATCC 29236) and cultured in modified chopped-meat medium (ATCC medium 1490; ELITE-MEDIA) at 37°C under anaerobic conditions.

    Techniques: Infection, Expressing, Isolation, Mouse Assay, Flow Cytometry, Staining, Injection, Two Tailed Test

    B. coccoides colonization promotes type I IFN and ISG responses in mononuclear phagocytes to limit EMCV infection. (A) Ifnb and antiviral defense gene expression in BMDMs isolated from PBS-treated, Abx-treated, or B. coccoides - or C. butyricum -colonized Abx-treated ice at 8 h after EMCV infection (MOI = 5) in vitro ( n = 5 or 6). (B) Relative mRNA expression of Oas1a and Mx1 in BMDMs isolated from WT or Ifnar −/− mice that pretreated with PBS, Abx, or Abx plus B. coccoides colonization at 8 hpi in vitro ( n = 5 or 6). (C) Splenic mononuclear phagocytes isolated from spleens of PBS-treated, Abx-treated, or B. coccoides -colonized Abx-treated mice were stimulated with 200 ng/ml IFN-γ in vitro . The data are the averaged frequencies of pSTAT1-stained CD11b + cell subsets at 16 h minus the frequency of these cells tested at 0 h after IFN-γ stimulation (3 experiments; n = 6). (D) Ifnb and antiviral defense gene expression in splenic mononuclear phagocytes isolated from EMCV-infected PBS-treated, Abx-treated, or B. coccoides -colonized Abx-treated mice at 1 dpi ( n = 6). (E) Relative mRNA expression of Cxcl10 and Stat2 in splenic mononuclear phagocytes isolated from EMCV-infected WT or Ifnar −/− mice pretreated with PBS, Abx, or Abx plus B. coccoides colonization at 1 dpi ( n = 5 or 6). Data are from at least two independent experiments. *, P

    Journal: mBio

    Article Title: The Intestinal Microbiome Primes Host Innate Immunity against Enteric Virus Systemic Infection through Type I Interferon

    doi: 10.1128/mBio.00366-21

    Figure Lengend Snippet: B. coccoides colonization promotes type I IFN and ISG responses in mononuclear phagocytes to limit EMCV infection. (A) Ifnb and antiviral defense gene expression in BMDMs isolated from PBS-treated, Abx-treated, or B. coccoides - or C. butyricum -colonized Abx-treated ice at 8 h after EMCV infection (MOI = 5) in vitro ( n = 5 or 6). (B) Relative mRNA expression of Oas1a and Mx1 in BMDMs isolated from WT or Ifnar −/− mice that pretreated with PBS, Abx, or Abx plus B. coccoides colonization at 8 hpi in vitro ( n = 5 or 6). (C) Splenic mononuclear phagocytes isolated from spleens of PBS-treated, Abx-treated, or B. coccoides -colonized Abx-treated mice were stimulated with 200 ng/ml IFN-γ in vitro . The data are the averaged frequencies of pSTAT1-stained CD11b + cell subsets at 16 h minus the frequency of these cells tested at 0 h after IFN-γ stimulation (3 experiments; n = 6). (D) Ifnb and antiviral defense gene expression in splenic mononuclear phagocytes isolated from EMCV-infected PBS-treated, Abx-treated, or B. coccoides -colonized Abx-treated mice at 1 dpi ( n = 6). (E) Relative mRNA expression of Cxcl10 and Stat2 in splenic mononuclear phagocytes isolated from EMCV-infected WT or Ifnar −/− mice pretreated with PBS, Abx, or Abx plus B. coccoides colonization at 1 dpi ( n = 5 or 6). Data are from at least two independent experiments. *, P

    Article Snippet: B. coccoides was purchased from ATCC (ATCC 29236) and cultured in modified chopped-meat medium (ATCC medium 1490; ELITE-MEDIA) at 37°C under anaerobic conditions.

    Techniques: Infection, Expressing, Isolation, In Vitro, Mouse Assay, Staining

    B. coccoides protects EMCV systemic infection by restricting viral in vivo replication. Viral titers (A) and induction of Ifnb (B) in the brains of PBS-, Abx-, or single antibiotic-treated mice were measured by qPCR at 3 dpi ( n = 5 or 6). (C) Bacterial richness was defined by the number of unique taxa. (D) Heat map of relative abundance from different antibiotic treatment groups. (E) Relative abundance changes of bacteria at the genus level. (F) Survival analysis of Abx-treated mice colonized with B. coccoides (B.C), A. muciniphila (AKK), L. reuteri (L.R), C. butyricum (C.B), or mouse fecal contents (FMT) following EMCV intraperitoneal inoculation (3 experiments; n = 16 to 20). (G) Viral burden in brain tissues harvested from mock-infected or infected mice that were PBS treated, Abx treated, or Abx treated and colonized with FMT, B. coccoides , or C. butyricum at 3 dpi ( n = 6). All data are from at least two independent experiments. *, P

    Journal: mBio

    Article Title: The Intestinal Microbiome Primes Host Innate Immunity against Enteric Virus Systemic Infection through Type I Interferon

    doi: 10.1128/mBio.00366-21

    Figure Lengend Snippet: B. coccoides protects EMCV systemic infection by restricting viral in vivo replication. Viral titers (A) and induction of Ifnb (B) in the brains of PBS-, Abx-, or single antibiotic-treated mice were measured by qPCR at 3 dpi ( n = 5 or 6). (C) Bacterial richness was defined by the number of unique taxa. (D) Heat map of relative abundance from different antibiotic treatment groups. (E) Relative abundance changes of bacteria at the genus level. (F) Survival analysis of Abx-treated mice colonized with B. coccoides (B.C), A. muciniphila (AKK), L. reuteri (L.R), C. butyricum (C.B), or mouse fecal contents (FMT) following EMCV intraperitoneal inoculation (3 experiments; n = 16 to 20). (G) Viral burden in brain tissues harvested from mock-infected or infected mice that were PBS treated, Abx treated, or Abx treated and colonized with FMT, B. coccoides , or C. butyricum at 3 dpi ( n = 6). All data are from at least two independent experiments. *, P

    Article Snippet: B. coccoides was purchased from ATCC (ATCC 29236) and cultured in modified chopped-meat medium (ATCC medium 1490; ELITE-MEDIA) at 37°C under anaerobic conditions.

    Techniques: Infection, In Vivo, Mouse Assay, Real-time Polymerase Chain Reaction

    Rarefaction curves for “pan” (A) and “core” (B) genes. The curves were fitted to median values of 1,000 iterations following the Power Law previously described ( Tettelin et al., 2008 ). The Shadows indicate 95% confidence intervals. (C) Distribution of the 43545 pan genes among the 224 genomes of the Blautia dataset. The bar representing the core genes is highlighted with red color. Inset: Percentages for each gene category in the pangenome, see Table 2 for definitions.

    Journal: Frontiers in Microbiology

    Article Title: Insights on the Evolutionary Genomics of the Blautia Genus: Potential New Species and Genetic Content Among Lineages

    doi: 10.3389/fmicb.2021.660920

    Figure Lengend Snippet: Rarefaction curves for “pan” (A) and “core” (B) genes. The curves were fitted to median values of 1,000 iterations following the Power Law previously described ( Tettelin et al., 2008 ). The Shadows indicate 95% confidence intervals. (C) Distribution of the 43545 pan genes among the 224 genomes of the Blautia dataset. The bar representing the core genes is highlighted with red color. Inset: Percentages for each gene category in the pangenome, see Table 2 for definitions.

    Article Snippet: Reclassification of Blautia coccoides as Blautia producta Subspecies coccoides and Emended Description of Blautia producta On the basis of genomic parameters, such as ANI, TETRA and AAI, as well as by phylogenomic profiling, the type strain of this former species (ATCC 29236 = DSM 935 = JCM 1395 = NCTC 11035) is reclassified as a subspecies of Blautia producta.

    Techniques:

    Phylogenomic tree from selected representative genomes from the clusters of genomic species from the Blautia dataset. The tree was computed using maximum likelihood, from the concatenated sequence alignment of 190 conserved single-copy orthogroups. The numeric data in format n/n represent values for bootstrap support (UF-bootstrap) and approximate likelihood-ratio test values (SH-aLRT). The tree is rooted using Robinsoniella peorensis data as the outgroup. The arbitrarily designed lineages I to VI are also shown. The color of each taxon name reflect the status of the current representative genome: red: undescribed potential new species; blue: type strain of a validated species; green: strain of a validated species, but not a type strain; brown: proposed type strain from a non-officially accepted species; black: current strains reassigned into another species. See text for more details.

    Journal: Frontiers in Microbiology

    Article Title: Insights on the Evolutionary Genomics of the Blautia Genus: Potential New Species and Genetic Content Among Lineages

    doi: 10.3389/fmicb.2021.660920

    Figure Lengend Snippet: Phylogenomic tree from selected representative genomes from the clusters of genomic species from the Blautia dataset. The tree was computed using maximum likelihood, from the concatenated sequence alignment of 190 conserved single-copy orthogroups. The numeric data in format n/n represent values for bootstrap support (UF-bootstrap) and approximate likelihood-ratio test values (SH-aLRT). The tree is rooted using Robinsoniella peorensis data as the outgroup. The arbitrarily designed lineages I to VI are also shown. The color of each taxon name reflect the status of the current representative genome: red: undescribed potential new species; blue: type strain of a validated species; green: strain of a validated species, but not a type strain; brown: proposed type strain from a non-officially accepted species; black: current strains reassigned into another species. See text for more details.

    Article Snippet: Reclassification of Blautia coccoides as Blautia producta Subspecies coccoides and Emended Description of Blautia producta On the basis of genomic parameters, such as ANI, TETRA and AAI, as well as by phylogenomic profiling, the type strain of this former species (ATCC 29236 = DSM 935 = JCM 1395 = NCTC 11035) is reclassified as a subspecies of Blautia producta.

    Techniques: Sequencing

    Gain/loss profile among different ancestors and lineages among the representative set of Blautia genomic species. The same tree (with the same members) shown in Figure 1 , was presented as a cladogram, and the data from Count analysis of gene gain/loss models, following Wagner parsimony model for 12,691 gene families, was represented. The roman numbers near each branch represent each proposed lineage among the members. Black numbers represent the number of shared families for a given node (i.e., predicted ancestor), as well as the green and red numbers represent the number of gene families gained and lost for the ancestor. The green and red numbers on the upper side of each terminal branch represented the same terms as before, but for the members of the tree (the terminal nodes). See text for more details.

    Journal: Frontiers in Microbiology

    Article Title: Insights on the Evolutionary Genomics of the Blautia Genus: Potential New Species and Genetic Content Among Lineages

    doi: 10.3389/fmicb.2021.660920

    Figure Lengend Snippet: Gain/loss profile among different ancestors and lineages among the representative set of Blautia genomic species. The same tree (with the same members) shown in Figure 1 , was presented as a cladogram, and the data from Count analysis of gene gain/loss models, following Wagner parsimony model for 12,691 gene families, was represented. The roman numbers near each branch represent each proposed lineage among the members. Black numbers represent the number of shared families for a given node (i.e., predicted ancestor), as well as the green and red numbers represent the number of gene families gained and lost for the ancestor. The green and red numbers on the upper side of each terminal branch represented the same terms as before, but for the members of the tree (the terminal nodes). See text for more details.

    Article Snippet: Reclassification of Blautia coccoides as Blautia producta Subspecies coccoides and Emended Description of Blautia producta On the basis of genomic parameters, such as ANI, TETRA and AAI, as well as by phylogenomic profiling, the type strain of this former species (ATCC 29236 = DSM 935 = JCM 1395 = NCTC 11035) is reclassified as a subspecies of Blautia producta.

    Techniques:

    Taxonomic abundance profile of the top five HGT donors for the Blautia genomes from the representative dataset. Each genome was analyzed by HGTector and the frequency of taxonomic assignments for the top 5 putative donors were plotted for each case. The color code corresponds to the donors listed in the legend (upper right side).

    Journal: Frontiers in Microbiology

    Article Title: Insights on the Evolutionary Genomics of the Blautia Genus: Potential New Species and Genetic Content Among Lineages

    doi: 10.3389/fmicb.2021.660920

    Figure Lengend Snippet: Taxonomic abundance profile of the top five HGT donors for the Blautia genomes from the representative dataset. Each genome was analyzed by HGTector and the frequency of taxonomic assignments for the top 5 putative donors were plotted for each case. The color code corresponds to the donors listed in the legend (upper right side).

    Article Snippet: Reclassification of Blautia coccoides as Blautia producta Subspecies coccoides and Emended Description of Blautia producta On the basis of genomic parameters, such as ANI, TETRA and AAI, as well as by phylogenomic profiling, the type strain of this former species (ATCC 29236 = DSM 935 = JCM 1395 = NCTC 11035) is reclassified as a subspecies of Blautia producta.

    Techniques: