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Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ <t>agglutinin</t> (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.
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Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ <t>agglutinin</t> (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.
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Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ <t>agglutinin</t> (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.
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Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ <t>agglutinin</t> (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.
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Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ <t>agglutinin</t> (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.
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Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ <t>agglutinin</t> (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.
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Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ agglutinin (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.

Journal: Physiological Reports

Article Title: Fibroblast Has2 limits acute heart failure following myocardial infarction in male mice

doi: 10.14814/phy2.70611

Figure Lengend Snippet: Fibroblast‐specific Has2 deletion does not cause changes in cardiomyocyte hypertrophy in mice 7 days post‐MI. We assessed the impact of Has2 deletion on cardiomyocyte size by wheat germ agglutinin (WGA) and measuring heart weights. (a) WGA‐stained (red) Has2 +/+ and Has2 −/− hearts. (b) Isolectin B4‐stained (green) hearts to determine vascular density. (c) Quantification of WGA‐stained Has2 +/+ ( n = 14; 7M, 7F) and Has2 −/− ( n = 19; 7M, 12F) hearts. Heart weight/tibia length ratio for Has2 +/+ ( n = 30; 15M, 16F) and Has2 −/− ( n = 39; 17M, 22F) hearts. Quantification of vascular density in isolectin B4‐stained Has2 +/+ ( n = 16; 8M, 8F) and Has2 −/− ( n = 20; 8M, 12F) hearts. (d) Males (e) Females.

Article Snippet: The borders of the cardiomyocytes were stained with wheat germ agglutinin (WGA CFTM 488A conjugate, Biotium) and the capillaries with Isolectin B4 DyLight 649 (Vector Labs DL‐1208‐.5) in 10 mM HEPES solution with calcium chloride dihydrate.

Techniques: Staining