minelute  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    MinElute PCR Purification Kit
    Description:
    For purification of up to 5 μg PCR products 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute PCR Purification Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Silica Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Purification of up to 5μg PCR Products in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers 2mL Collection Tubes Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    Catalog Number:
    28004
    Price:
    134
    Category:
    MinElute PCR Purification Kit
    Buy from Supplier


    Structured Review

    Qiagen minelute
    MinElute PCR Purification Kit
    For purification of up to 5 μg PCR products 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute PCR Purification Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Silica Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Purification of up to 5μg PCR Products in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers 2mL Collection Tubes Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/minelute/product/Qiagen
    Average 90 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    minelute - by Bioz Stars, 2020-01
    90/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: .. The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells. .. For restriction fragment length polymorphism (RFLP) and sequencing, the inserted fragment was PCR amplified with the vector-specific primers T7 and SP6.

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit. .. Transformants were screened by in situ PCRs using RedTaq ReadyMix PCR mix (Sigma-Aldrich, Vienna, Austria); recombinant clones were analyzed by restriction mapping and confirmed by sequencing (Agowa, Berlin, Germany).

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: Paragraph title: Verification of mutation entry clones by barcoded next-generation sequencing • TIMING 5-6 d hands-on, 2-3 weeks expansion ... 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer.

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: Paragraph title: Cloning and sequencing. ... Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA).

    Amplification:

    Article Title: Phase I and II Study of the Safety, Virologic Effect, and Pharmacokinetics/Pharmacodynamics of Single-Dose 3-O-(3?,3?-Dimethylsuccinyl)Betulinic Acid (Bevirimat) against Human Immunodeficiency Virus Infection ▿
    Article Snippet: Viral RNA was purified from patient plasma using the QIAamp Mini viral RNA purification kit (QIAGEN). gag cDNA was synthesized by reverse transcription-PCR using the StrataScript first-strand synthesis system (Stratagene) for reverse transcription, followed by amplification of double-stranded DNA using the PicoMaxx high-fidelity PCR master mix (Stratagene). .. A final product of approximately 1 kb was purified using the MinElute PCR purification kit (QIAGEN).

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx. .. All PCR amplicons and library amplification primers are listed in Table .

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions. .. Following DNA purification, 1μl of eluted DNA was used in a qPCR reaction to estimate the optimum number of amplification cycles.

    Article Title: Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
    Article Snippet: Paragraph title: DNA extraction and amplification ... After decalcification and digestion, the supernatant was concentrated to about 100 μl using Vivaspin filter 3000 MWCO (Sartorius Stedim Biotech), and then directly purified using silica based spin columns (Minelute PCR Purification kit, QIAGEN, Inc).

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells. .. For restriction fragment length polymorphism (RFLP) and sequencing, the inserted fragment was PCR amplified with the vector-specific primers T7 and SP6.

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: .. PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit. .. E. coli transformation was done according to the manufacturer's instructions (Invitrogen).

    Article Title: DNA Diagnostics by Surface-Bound Melt-Curve Reactions
    Article Snippet: Fifty-μl PCR products were purified using a MinElute PCR purification kit (Qiagen) following the manufacturer’s protocol and eluting the PCR products in water. .. One μl of each eluted product (∼25 ng) was used as template for cycle sequencing that used a BigDye 3.1 terminator kit (Applied Biosystems), using 0.16 μmol/L of either of the initial amplification primers in 20-μl reactions.

    Article Title: Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1 2
    Article Snippet: Paragraph title: Polymerase chain reaction exon amplification ... PCR products, which were confirmed to have a single target band in a 2% agarose gel, were purified using the Qiagen MinElute PCR Purification Kit (Qiagen, Valencia, CA).

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: QPCR amplicons produced from treatment column sands on days 28 and 32 were chosen for cloning and sequencing to confirm that there was amplification of the intended target. .. Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA).

    Synthesized:

    Article Title: Phase I and II Study of the Safety, Virologic Effect, and Pharmacokinetics/Pharmacodynamics of Single-Dose 3-O-(3?,3?-Dimethylsuccinyl)Betulinic Acid (Bevirimat) against Human Immunodeficiency Virus Infection ▿
    Article Snippet: Viral RNA was purified from patient plasma using the QIAamp Mini viral RNA purification kit (QIAGEN). gag cDNA was synthesized by reverse transcription-PCR using the StrataScript first-strand synthesis system (Stratagene) for reverse transcription, followed by amplification of double-stranded DNA using the PicoMaxx high-fidelity PCR master mix (Stratagene). .. A final product of approximately 1 kb was purified using the MinElute PCR purification kit (QIAGEN).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: At day six, cells were detached from the plate, and 1×105 macrophages were reseeded in 96-well flat bottom plates to be re- stimulated for 24 hours with 200 µl of either RPMI or Escherichia coli LPS (serotype 055:B5, Sigma-Aldrich, 10 ng/ml), after which supernatants were collected and stored at −20o C. Cytokine production was determined in supernatants using commercial ELISA kits for TNFα and IL-6 (R & D systems) following the instructions of the manufacturer. .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method.

    Modification:

    Article Title: Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
    Article Snippet: DNA from both pig and cattle bones and teeth was extracted according to a method modified from Yang et al . .. After decalcification and digestion, the supernatant was concentrated to about 100 μl using Vivaspin filter 3000 MWCO (Sartorius Stedim Biotech), and then directly purified using silica based spin columns (Minelute PCR Purification kit, QIAGEN, Inc).

    Incubation:

    Article Title: Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
    Article Snippet: Specifically, 1 ml of extraction buffer [0.44 M EDTA (pH = 8) (AMRESCO, USA), 0.1 M urea, and 20 mg/ml proteinase K (AMRESCO, USA)] was added to about 50 mg of bone powder and incubated overnight at 56 °C. .. After decalcification and digestion, the supernatant was concentrated to about 100 μl using Vivaspin filter 3000 MWCO (Sartorius Stedim Biotech), and then directly purified using silica based spin columns (Minelute PCR Purification kit, QIAGEN, Inc).

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: The reaction mixtures were incubated in a MyCycler (Bio-Rad) under the following conditions: initial denaturation at 94°C for 5 min; 30 cycles, with 1 cycle consisting of 94°C for 1 min, 45°C for 45 s, and 72°C for 90 s; followed by one cycle of 72°C for 2 min. .. The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells.

    Article Title: Identification of Escherichia coli O157 by Using a Novel Colorimetric Detection Method with DNA Microarrays
    Article Snippet: For each hybridization reaction, 45 μL of PCR amplicons was purified by using the MinElute® PCR purification kit (Qiagen, Valencia, CA), and 20 μL of the eluate was digested with 10 U of lambda exonuclease and 1 × lambda exonuclease reaction buffer (Epicenter Biotechnologies, Madison, WI) in a final volume of 23 μL for 5 min at 37°C, followed by immediate addition of an equal volume of InDevR 2 × Hyb Buffer (InDevR, Inc.). .. The hybridization mixture was applied to each microarray that was previously rinsed with distilled water for 5 min, and the microarray slides were further incubated for 1 h at room temperature.

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: Monocytes (1×107 ) were plated to 10 cm Petri dishes (Greiner) in 10 ml medium volumes and incubated with either culture medium only as a negative control or 5 µg/ml of β- glucan with or without mTORi-HDL (1 µg/ml) for 24 hours (in 10% pooled human serum). .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method.

    Article Title: Transcriptome Analysis of Lactococcus lactis in Coculture with Saccharomyces cerevisiae ▿
    Article Snippet: The cDNA was purified with columns provided by a MinElute PCR purification kit (Qiagen). .. The cDNA was transferred to aliquots of Cy3 and Cy5 monofunctional NHS-esters (Amersham Biosciences Europe) and incubated for 60 min in the dark.

    BIA-KA:

    Article Title: In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
    Article Snippet: QIAquick PCR Purification kit and MinElute PCR Purification kit were purchased from QIAGEN. .. T4 RNA ligase, Bca BEST RNA PCR kit ver1.1, GelStar Nucleic Acid Stain and ribonuclease inhibitor were from TaKaRa BIO.

    Ex Vivo:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: In addition, ATAC-seq was performed on splenic total CD19+ CD21hi CD24hi B cells isolated as above for the RNA-seq from Mb1 cre/+ and Ahr fl/- Mb1 cre/+ mice either left untouched (ex-vivo) or stimulated with LPS+anti-IgM for 6h in IMDM media. .. DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions.

    Transformation Assay:

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: Plasmid DNA from transformed cells was isolated with a QIAGEN plasmid miniprep kit. .. PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit.

    Hybridization:

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx. .. All PCR amplicons and library amplification primers are listed in Table .

    Article Title: Identification of Escherichia coli O157 by Using a Novel Colorimetric Detection Method with DNA Microarrays
    Article Snippet: .. For each hybridization reaction, 45 μL of PCR amplicons was purified by using the MinElute® PCR purification kit (Qiagen, Valencia, CA), and 20 μL of the eluate was digested with 10 U of lambda exonuclease and 1 × lambda exonuclease reaction buffer (Epicenter Biotechnologies, Madison, WI) in a final volume of 23 μL for 5 min at 37°C, followed by immediate addition of an equal volume of InDevR 2 × Hyb Buffer (InDevR, Inc.). .. The hybridization mixture was applied to each microarray that was previously rinsed with distilled water for 5 min, and the microarray slides were further incubated for 1 h at room temperature.

    Transfection:

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: Deep sequencing was performed on multiplexed PCR amplicons from gDNA harvested from plasmid transfection of HEK293T cells. .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx.

    Ligation:

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx. .. All PCR amplicons and library amplification primers are listed in Table .

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer. .. 37 Multiplex up to ~100 pools by ligation to unique barcode adaptors, according to manufacturer’s instructions.

    SYBR Green Assay:

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method. ..

    Transferring:

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: This can be done by transferring all PCR products in each well of 96-well plates into a common reservoir, and mixing well by gentle shaking or pipetting. .. 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer.

    Generated:

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: Note that if only a few mutant clones need to be confirmed, traditional Sanger sequencing with walking primers may be more cost effective, and can be used to sequence the PCR products generated in step 34 . .. 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer.

    Sequencing:

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx. .. All PCR amplicons and library amplification primers are listed in Table .

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: The cell pellet was prepped for sequencing by using the Nextera DNA library preparation kit (Illumina). .. DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions.

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells. .. For restriction fragment length polymorphism (RFLP) and sequencing, the inserted fragment was PCR amplified with the vector-specific primers T7 and SP6.

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit. .. Transformants were screened by in situ PCRs using RedTaq ReadyMix PCR mix (Sigma-Aldrich, Vienna, Austria); recombinant clones were analyzed by restriction mapping and confirmed by sequencing (Agowa, Berlin, Germany).

    Article Title: A CHAF1B-dependent molecular switch in hematopoiesis and leukemia pathogenesis
    Article Snippet: After transposition, DNA fragments were collected using a Qiagen MinElute PCR purification kit. .. Sequencing libraries were prepared and controlled for quality as previously described ( ).

    Article Title: DNA Diagnostics by Surface-Bound Melt-Curve Reactions
    Article Snippet: Paragraph title: Sequencing ... Fifty-μl PCR products were purified using a MinElute PCR purification kit (Qiagen) following the manufacturer’s protocol and eluting the PCR products in water.

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: This facilitates the mutant clone sequence assembly downstream of next-generation sequencing. .. 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer.

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: Paragraph title: Cloning and sequencing. ... Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA).

    Sonication:

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: The remaining cells were fixed in 1% methanol-free formaldehyde and sonicated. .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method.

    Recombinant:

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit. .. Transformants were screened by in situ PCRs using RedTaq ReadyMix PCR mix (Sigma-Aldrich, Vienna, Austria); recombinant clones were analyzed by restriction mapping and confirmed by sequencing (Agowa, Berlin, Germany).

    Staining:

    Article Title: In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
    Article Snippet: QIAquick PCR Purification kit and MinElute PCR Purification kit were purchased from QIAGEN. .. T4 RNA ligase, Bca BEST RNA PCR kit ver1.1, GelStar Nucleic Acid Stain and ribonuclease inhibitor were from TaKaRa BIO.

    DNA Extraction:

    Article Title: Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
    Article Snippet: Paragraph title: DNA extraction and amplification ... After decalcification and digestion, the supernatant was concentrated to about 100 μl using Vivaspin filter 3000 MWCO (Sartorius Stedim Biotech), and then directly purified using silica based spin columns (Minelute PCR Purification kit, QIAGEN, Inc).

    Nucleic Acid Electrophoresis:

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells. .. Restricted fragments were analyzed by gel electrophoresis, and restriction patterns were compared visually.

    RNA Sequencing Assay:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: In addition, ATAC-seq was performed on splenic total CD19+ CD21hi CD24hi B cells isolated as above for the RNA-seq from Mb1 cre/+ and Ahr fl/- Mb1 cre/+ mice either left untouched (ex-vivo) or stimulated with LPS+anti-IgM for 6h in IMDM media. .. DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions.

    Mutagenesis:

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: Paragraph title: Verification of mutation entry clones by barcoded next-generation sequencing • TIMING 5-6 d hands-on, 2-3 weeks expansion ... 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer.

    Isolation:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: In addition, ATAC-seq was performed on splenic total CD19+ CD21hi CD24hi B cells isolated as above for the RNA-seq from Mb1 cre/+ and Ahr fl/- Mb1 cre/+ mice either left untouched (ex-vivo) or stimulated with LPS+anti-IgM for 6h in IMDM media. .. DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions.

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: Plasmid DNA from transformed cells was isolated with a QIAGEN plasmid miniprep kit. .. PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit.

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method. ..

    Multiplex Assay:

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx. .. All PCR amplicons and library amplification primers are listed in Table .

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer. .. 37 Multiplex up to ~100 pools by ligation to unique barcode adaptors, according to manufacturer’s instructions.

    Labeling:

    Article Title: In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
    Article Snippet: 5′-Phospho-deoxycytidyl-phospho-puromycin (pdCp-puromycin) and a fluorescently labeled primer BFL-YF515 were from Japan Bio Services. .. QIAquick PCR Purification kit and MinElute PCR Purification kit were purchased from QIAGEN.

    Article Title: Transcriptome Analysis of Lactococcus lactis in Coculture with Saccharomyces cerevisiae ▿
    Article Snippet: Paragraph title: Reverse transcription and labeling. ... The cDNA was purified with columns provided by a MinElute PCR purification kit (Qiagen).

    Purification:

    Article Title: In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
    Article Snippet: .. QIAquick PCR Purification kit and MinElute PCR Purification kit were purchased from QIAGEN. .. KOD Dash DNA polymerase was from TOYOBO.

    Article Title: Phase I and II Study of the Safety, Virologic Effect, and Pharmacokinetics/Pharmacodynamics of Single-Dose 3-O-(3?,3?-Dimethylsuccinyl)Betulinic Acid (Bevirimat) against Human Immunodeficiency Virus Infection ▿
    Article Snippet: .. A final product of approximately 1 kb was purified using the MinElute PCR purification kit (QIAGEN). ..

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx. .. All PCR amplicons and library amplification primers are listed in Table .

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: .. DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions. .. Following DNA purification, 1μl of eluted DNA was used in a qPCR reaction to estimate the optimum number of amplification cycles.

    Article Title: Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
    Article Snippet: .. After decalcification and digestion, the supernatant was concentrated to about 100 μl using Vivaspin filter 3000 MWCO (Sartorius Stedim Biotech), and then directly purified using silica based spin columns (Minelute PCR Purification kit, QIAGEN, Inc). .. Short fragments of the mtDNA CR were amplified.

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: .. The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells. .. For restriction fragment length polymorphism (RFLP) and sequencing, the inserted fragment was PCR amplified with the vector-specific primers T7 and SP6.

    Article Title: Identification of Escherichia coli O157 by Using a Novel Colorimetric Detection Method with DNA Microarrays
    Article Snippet: .. For each hybridization reaction, 45 μL of PCR amplicons was purified by using the MinElute® PCR purification kit (Qiagen, Valencia, CA), and 20 μL of the eluate was digested with 10 U of lambda exonuclease and 1 × lambda exonuclease reaction buffer (Epicenter Biotechnologies, Madison, WI) in a final volume of 23 μL for 5 min at 37°C, followed by immediate addition of an equal volume of InDevR 2 × Hyb Buffer (InDevR, Inc.). .. The hybridization mixture was applied to each microarray that was previously rinsed with distilled water for 5 min, and the microarray slides were further incubated for 1 h at room temperature.

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: .. PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit. .. E. coli transformation was done according to the manufacturer's instructions (Invitrogen).

    Article Title: A CHAF1B-dependent molecular switch in hematopoiesis and leukemia pathogenesis
    Article Snippet: .. After transposition, DNA fragments were collected using a Qiagen MinElute PCR purification kit. .. Sequencing libraries were prepared and controlled for quality as previously described ( ).

    Article Title: DNA Diagnostics by Surface-Bound Melt-Curve Reactions
    Article Snippet: .. Fifty-μl PCR products were purified using a MinElute PCR purification kit (Qiagen) following the manufacturer’s protocol and eluting the PCR products in water. .. One μl of each eluted product (∼25 ng) was used as template for cycle sequencing that used a BigDye 3.1 terminator kit (Applied Biosystems), using 0.16 μmol/L of either of the initial amplification primers in 20-μl reactions.

    Article Title: Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1 2
    Article Snippet: .. PCR products, which were confirmed to have a single target band in a 2% agarose gel, were purified using the Qiagen MinElute PCR Purification Kit (Qiagen, Valencia, CA). .. The purified products were then subjected to direct sequencing by an ABI377 sequencer (Perkin-Elmer Applied Biosystems, Foster City, CA).

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: .. 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer. .. 37 Multiplex up to ~100 pools by ligation to unique barcode adaptors, according to manufacturer’s instructions.

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method. ..

    Article Title: Transcriptome Analysis of Lactococcus lactis in Coculture with Saccharomyces cerevisiae ▿
    Article Snippet: .. The cDNA was purified with columns provided by a MinElute PCR purification kit (Qiagen). .. The columns were washed with 70% ethanol, and the cDNA was eluted with 10 μl of EB buffer (LabelStar kit; Qiagen).

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: .. Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA). .. A TOPO-TA cloning kit for sequencing using the pCR 4.0 vector and Mach competent cells was used according to the manufacturer's protocols (Invitrogen, Carlsbad, CA).

    Polymerase Chain Reaction:

    Article Title: In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
    Article Snippet: .. QIAquick PCR Purification kit and MinElute PCR Purification kit were purchased from QIAGEN. .. KOD Dash DNA polymerase was from TOYOBO.

    Article Title: Phase I and II Study of the Safety, Virologic Effect, and Pharmacokinetics/Pharmacodynamics of Single-Dose 3-O-(3?,3?-Dimethylsuccinyl)Betulinic Acid (Bevirimat) against Human Immunodeficiency Virus Infection ▿
    Article Snippet: .. A final product of approximately 1 kb was purified using the MinElute PCR purification kit (QIAGEN). ..

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx. .. All PCR amplicons and library amplification primers are listed in Table .

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: .. DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions. .. Following DNA purification, 1μl of eluted DNA was used in a qPCR reaction to estimate the optimum number of amplification cycles.

    Article Title: Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
    Article Snippet: .. After decalcification and digestion, the supernatant was concentrated to about 100 μl using Vivaspin filter 3000 MWCO (Sartorius Stedim Biotech), and then directly purified using silica based spin columns (Minelute PCR Purification kit, QIAGEN, Inc). .. Short fragments of the mtDNA CR were amplified.

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: .. The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells. .. For restriction fragment length polymorphism (RFLP) and sequencing, the inserted fragment was PCR amplified with the vector-specific primers T7 and SP6.

    Article Title: Identification of Escherichia coli O157 by Using a Novel Colorimetric Detection Method with DNA Microarrays
    Article Snippet: .. For each hybridization reaction, 45 μL of PCR amplicons was purified by using the MinElute® PCR purification kit (Qiagen, Valencia, CA), and 20 μL of the eluate was digested with 10 U of lambda exonuclease and 1 × lambda exonuclease reaction buffer (Epicenter Biotechnologies, Madison, WI) in a final volume of 23 μL for 5 min at 37°C, followed by immediate addition of an equal volume of InDevR 2 × Hyb Buffer (InDevR, Inc.). .. The hybridization mixture was applied to each microarray that was previously rinsed with distilled water for 5 min, and the microarray slides were further incubated for 1 h at room temperature.

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: .. PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit. .. E. coli transformation was done according to the manufacturer's instructions (Invitrogen).

    Article Title: A CHAF1B-dependent molecular switch in hematopoiesis and leukemia pathogenesis
    Article Snippet: .. After transposition, DNA fragments were collected using a Qiagen MinElute PCR purification kit. .. Sequencing libraries were prepared and controlled for quality as previously described ( ).

    Article Title: DNA Diagnostics by Surface-Bound Melt-Curve Reactions
    Article Snippet: .. Fifty-μl PCR products were purified using a MinElute PCR purification kit (Qiagen) following the manufacturer’s protocol and eluting the PCR products in water. .. One μl of each eluted product (∼25 ng) was used as template for cycle sequencing that used a BigDye 3.1 terminator kit (Applied Biosystems), using 0.16 μmol/L of either of the initial amplification primers in 20-μl reactions.

    Article Title: Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1 2
    Article Snippet: .. PCR products, which were confirmed to have a single target band in a 2% agarose gel, were purified using the Qiagen MinElute PCR Purification Kit (Qiagen, Valencia, CA). .. The purified products were then subjected to direct sequencing by an ABI377 sequencer (Perkin-Elmer Applied Biosystems, Foster City, CA).

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: .. 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer. .. 37 Multiplex up to ~100 pools by ligation to unique barcode adaptors, according to manufacturer’s instructions.

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method. ..

    Article Title: Transcriptome Analysis of Lactococcus lactis in Coculture with Saccharomyces cerevisiae ▿
    Article Snippet: .. The cDNA was purified with columns provided by a MinElute PCR purification kit (Qiagen). .. The columns were washed with 70% ethanol, and the cDNA was eluted with 10 μl of EB buffer (LabelStar kit; Qiagen).

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: .. Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA). .. A TOPO-TA cloning kit for sequencing using the pCR 4.0 vector and Mach competent cells was used according to the manufacturer's protocols (Invitrogen, Carlsbad, CA).

    CRISPR:

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: The CRISPR cut site was within the first 100 bp of the amplicon (from either the 5′- or 3′-end) to ensure high-quality data. .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx.

    Concentration Assay:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions. .. DNA concentration was measured with a Qubit fluorometer (Life Technologies).

    Mouse Assay:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: In addition, ATAC-seq was performed on splenic total CD19+ CD21hi CD24hi B cells isolated as above for the RNA-seq from Mb1 cre/+ and Ahr fl/- Mb1 cre/+ mice either left untouched (ex-vivo) or stimulated with LPS+anti-IgM for 6h in IMDM media. .. DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity
    Article Snippet: Deep sequencing was performed on multiplexed PCR amplicons from gDNA harvested from plasmid transfection of HEK293T cells. .. The experiment was performed as follows: (1) PCR amplification of the genomic region that flanks the sgRNA on- or predicted off-target sites for each gene using PrimeSTAR GXL DNA Polymerase (TaKaRa); (2) hybridization mix preparation for adapters P5 (IS1 and IS3) and P7 (IS2 and IS3); (3) blunt end repair of DNA fragment using dNTPs, ATP, T4 polynucleotide kinase, and T4 DNA polymerase; (4) reaction product purification using MinElute PCR Purification Kit (QIAGEN); (5) adapter ligation and fill-in; (6) DNA library amplification by PCR using primer pairs inPE1.0 and inPE2.0 and Illumina multiplex primer; and (7) amplified DNA library sequencing by Illumina Genome Analyzer IIx.

    Article Title: Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ Subgroup in Pelagic Redoxclines of the Central Baltic Sea ▿ †
    Article Snippet: .. The PCR products were purified (MinElute PCR purification kit; Qiagen) and then cloned following the manufacturer's instructions using the pGEM-T Easy vector system (Promega) together with competent Escherichia coli JM109 cells. .. For restriction fragment length polymorphism (RFLP) and sequencing, the inserted fragment was PCR amplified with the vector-specific primers T7 and SP6.

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: Plasmid DNA from transformed cells was isolated with a QIAGEN plasmid miniprep kit. .. PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit.

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA). .. A TOPO-TA cloning kit for sequencing using the pCR 4.0 vector and Mach competent cells was used according to the manufacturer's protocols (Invitrogen, Carlsbad, CA).

    Real-time Polymerase Chain Reaction:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions. .. Following DNA purification, 1μl of eluted DNA was used in a qPCR reaction to estimate the optimum number of amplification cycles.

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method. ..

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: QPCR amplicons produced from treatment column sands on days 28 and 32 were chosen for cloning and sequencing to confirm that there was amplification of the intended target. .. Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA).

    Negative Control:

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: Monocytes (1×107 ) were plated to 10 cm Petri dishes (Greiner) in 10 ml medium volumes and incubated with either culture medium only as a negative control or 5 µg/ml of β- glucan with or without mTORi-HDL (1 µg/ml) for 24 hours (in 10% pooled human serum). .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method.

    Selection:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions. .. Library amplification was performed using custom Nextera primers and was followed by solid phase reversible immobilization (SPRI) size selection to exclude fragments larger than 1,200bp.

    Agarose Gel Electrophoresis:

    Article Title: Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1Molecular Analysis of a Multistep Lung Cancer Model Induced by Chronic Inflammation Reveals Epigenetic Regulation of p16 and Activation of the DNA Damage Response Pathway 1 2
    Article Snippet: .. PCR products, which were confirmed to have a single target band in a 2% agarose gel, were purified using the Qiagen MinElute PCR Purification Kit (Qiagen, Valencia, CA). .. The purified products were then subjected to direct sequencing by an ABI377 sequencer (Perkin-Elmer Applied Biosystems, Foster City, CA).

    In Situ:

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit. .. Transformants were screened by in situ PCRs using RedTaq ReadyMix PCR mix (Sigma-Aldrich, Vienna, Austria); recombinant clones were analyzed by restriction mapping and confirmed by sequencing (Agowa, Berlin, Germany).

    Microarray:

    Article Title: Identification of Escherichia coli O157 by Using a Novel Colorimetric Detection Method with DNA Microarrays
    Article Snippet: Paragraph title: Microarray hybridization ... For each hybridization reaction, 45 μL of PCR amplicons was purified by using the MinElute® PCR purification kit (Qiagen, Valencia, CA), and 20 μL of the eluate was digested with 10 U of lambda exonuclease and 1 × lambda exonuclease reaction buffer (Epicenter Biotechnologies, Madison, WI) in a final volume of 23 μL for 5 min at 37°C, followed by immediate addition of an equal volume of InDevR 2 × Hyb Buffer (InDevR, Inc.).

    Next-Generation Sequencing:

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: Paragraph title: Verification of mutation entry clones by barcoded next-generation sequencing • TIMING 5-6 d hands-on, 2-3 weeks expansion ... 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer.

    Ancient DNA Assay:

    Article Title: Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
    Article Snippet: DNA extraction and amplification DNA extractions and preparations for PCR reactions of the samples were carried out in a dedicated ancient DNA lab, at the Institute of Archaeology of Tel Aviv University, Israel. .. After decalcification and digestion, the supernatant was concentrated to about 100 μl using Vivaspin filter 3000 MWCO (Sartorius Stedim Biotech), and then directly purified using silica based spin columns (Minelute PCR Purification kit, QIAGEN, Inc).

    Random Hexamer Labeling:

    Article Title: Transcriptome Analysis of Lactococcus lactis in Coculture with Saccharomyces cerevisiae ▿
    Article Snippet: Each RT reaction was performed in 30-μl volumes containing 30 μg total RNA, 2 μl of random hexamer primers (Amersham), 0.8 mM dATP, 0.8 mM dCTP, 0.8 mM dGTP, 0.2 mM dTTP, 0.6 mM aa-dUTP (Sigma-Aldrich), 300 units of SuperscriptII reverse transcriptase (Invitrogen), 6 μl of Superscript IIA buffer (5×), 3 μl 100 mM dithiothreitol, and 0.4 μl of sterile water. .. The cDNA was purified with columns provided by a MinElute PCR purification kit (Qiagen).

    Spectrophotometry:

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: .. 36 Purify the pooled PCR products using MinElute PCR Purification Kit (Qiagen), following the manufacturer’s instructions, and quantify DNA content by a NanoDrop spectrophotometer. .. 37 Multiplex up to ~100 pools by ligation to unique barcode adaptors, according to manufacturer’s instructions.

    Produced:

    Article Title: Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿Growth of Enterococci in Unaltered, Unseeded Beach Sands Subjected to Tidal Wetting ▿ †
    Article Snippet: QPCR amplicons produced from treatment column sands on days 28 and 32 were chosen for cloning and sequencing to confirm that there was amplification of the intended target. .. Triplicate 25-μl reaction mixtures were pooled for each sample and purified using a MinElute PCR purification kit (Qiagen, Valencia, CA).

    Immunoprecipitation:

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: Immunoprecipitation was performed using an antibody against H3K4me3 (Diagenode, Seraing, Belgium). .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method.

    DNA Purification:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: DNA was then purified using a MinElute PCR purification kit (QIAGEN), according to manufacturer’s instructions. .. Following DNA purification, 1μl of eluted DNA was used in a qPCR reaction to estimate the optimum number of amplification cycles.

    Gel Extraction:

    Article Title: Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a ▿
    Article Snippet: A QIAGEN MinElute gel extraction kit was used to purify DNA fragments from agarose gels, and a QIAGEN MinElute reaction cleanup kit was used to purify digested oligonucleotides and plasmids. .. PCR conditions were optimized for each primer pair, and amplification products were purified using a QIAGEN MinElute PCR purification kit.

    Gradient Centrifugation:

    Article Title: Inhibiting inflammation with myeloid cell-specific nanobiologics promotes organ transplant acceptance
    Article Snippet: Subsequently, monocyte isolation was performed by hyper-osmotic density gradient centrifugation over Percoll (Sigma). .. DNA was isolated with a MinElute PCR purification kit (Quiagen) and was further processed for qPCR analysis using the SYBR green method.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Qiagen minelute pcr purification kit
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 2204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minelute pcr purification kit/product/Qiagen
    Average 90 stars, based on 2204 article reviews
    Price from $9.99 to $1999.99
    minelute pcr purification kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results