streptococcus sobrinus clinical  (ATCC)

Journal:

Article Title: Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque

doi: 10.1128/JCM.40.1.205-209.2002

Figure Lengend Snippet: Bacterial strains a used for testing glmM primer specificity

Article Snippet: Oligonucleotide primers glmMF, glmMR, and glmMI were tested for amplification specificity using DNA extracted from a panel of bacterial strains, as indicated in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial species Arcobacter butzleri clinical isolate A. cryaerophila clinical isolate Actinomyces naeslundii NCTC 10301 Bacillus cereus clinical isolate Campylobacter coli clinical isolate Campylobacter concisus EF 13144 Campylobacter fetus clinical isolate Campylobacter hyointestinalis NCTC 10608 Campylobacter jejuni clinical isolate Campylobacter lari clinical isolate Campylobacter mucosalis NCTC 11000 Campylobacter upsaliensis clinical isolate Enterobacter aerogenes ATCC 49469 Enterococcus faecalis ATCC 49474 Escherichia coli ATCC 13086 Klebsiella pneumoniae ATCC 49472 Lactobacillus paracasei clinical isolate Proteus mirabilis ATCC 12453 Prevotella intermedia ATCC 25611 Salmonella typhimurium ATCC 49469 Shigella sonnei ATCC8574 Shigella flexneri CCRC 10772 Streptococcus cricetus clinical isolate Streptococcus ferus clinical isolate Streptococcus macacae clinical isolate Streptococcus mutans ATCC 10449 Streptococcus mutans ATCC 25175 Streptococcus salivarius clinical isolate Streptococcus sobrinus clinical isolate Vibrio cholerae NCTC 5941 Helicobacter cinaedi NCTC 11611 H. fennelliae NCTC 11612 H. pylori ATCC 700392 H. pylori clinical isolate 102.90 H. pylori clinical isolate 114.90 H. pylori clinical isolate 201.90 H. pylori clinical isolate 206.90 Ig H. pylori clinical isolate 206.90 sm H. pylori clinical isolate 220.90 H. pylori clinical isolate 228.90 H. pylori clinical isolate 237.90 H. pylori clinical isolate 242.90 li H. pylori clinical isolate 242.90 da H. pylori clinical isolate 513.90 Open in a separate window a Clinical isolates were obtained from the Red Cross Children’s Hospital, Rondebosch, Cape Town, South Africa; South African Institute for Medical Research, Johannesburg, South Africa; Witwatersrand University Medical School, Johannesburg, South Africa; and the Council for Scientific and Industrial Research, Pretoria, South Africa.

Techniques:

Journal:

Article Title: Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque

doi: 10.1128/JCM.40.1.205-209.2002

Figure Lengend Snippet: Specific detection of H. pylori by heminested PCR. Shown is agarose gel electrophoresis of amplicons obtained from cells of Arcobacter butzleri, Campylobacter conciscus EF 13144, Campylobacter hyointestinalis NCTC 10608, Campylobacter mucosalis NCTC 1100, Lactobacillus paracasei, Streptococcus cricetus, Streptococcus salivarius, Streptococcus sobrinus, Helicobacter cinaedi NCTC 11611, Helicobacter fennelliae NCTC 11612, H. pylori ATCC 700392, H. pylori isolate 114.90, H. pylori isolate 206.90 sm, H. pylori isolate 228.90, and H. pylori isolate 242.90 Da (lanes 3 to 16, respectively). The arrow indicates the 496-bp amplified fragments obtained only with H. pylori (lanes 12 to 16). Lane 2, negative control (no DNA template). Lanes 1 and 17, 100-bp ladder as a molecular size standard (Promega).

Article Snippet: Oligonucleotide primers glmMF, glmMR, and glmMI were tested for amplification specificity using DNA extracted from a panel of bacterial strains, as indicated in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial species Arcobacter butzleri clinical isolate A. cryaerophila clinical isolate Actinomyces naeslundii NCTC 10301 Bacillus cereus clinical isolate Campylobacter coli clinical isolate Campylobacter concisus EF 13144 Campylobacter fetus clinical isolate Campylobacter hyointestinalis NCTC 10608 Campylobacter jejuni clinical isolate Campylobacter lari clinical isolate Campylobacter mucosalis NCTC 11000 Campylobacter upsaliensis clinical isolate Enterobacter aerogenes ATCC 49469 Enterococcus faecalis ATCC 49474 Escherichia coli ATCC 13086 Klebsiella pneumoniae ATCC 49472 Lactobacillus paracasei clinical isolate Proteus mirabilis ATCC 12453 Prevotella intermedia ATCC 25611 Salmonella typhimurium ATCC 49469 Shigella sonnei ATCC8574 Shigella flexneri CCRC 10772 Streptococcus cricetus clinical isolate Streptococcus ferus clinical isolate Streptococcus macacae clinical isolate Streptococcus mutans ATCC 10449 Streptococcus mutans ATCC 25175 Streptococcus salivarius clinical isolate Streptococcus sobrinus clinical isolate Vibrio cholerae NCTC 5941 Helicobacter cinaedi NCTC 11611 H. fennelliae NCTC 11612 H. pylori ATCC 700392 H. pylori clinical isolate 102.90 H. pylori clinical isolate 114.90 H. pylori clinical isolate 201.90 H. pylori clinical isolate 206.90 Ig H. pylori clinical isolate 206.90 sm H. pylori clinical isolate 220.90 H. pylori clinical isolate 228.90 H. pylori clinical isolate 237.90 H. pylori clinical isolate 242.90 li H. pylori clinical isolate 242.90 da H. pylori clinical isolate 513.90 Open in a separate window a Clinical isolates were obtained from the Red Cross Children’s Hospital, Rondebosch, Cape Town, South Africa; South African Institute for Medical Research, Johannesburg, South Africa; Witwatersrand University Medical School, Johannesburg, South Africa; and the Council for Scientific and Industrial Research, Pretoria, South Africa.

Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control

Journal: eLife

Article Title: TMEM79/MATTRIN defines a pathway for Frizzled regulation and is required for Xenopus embryogenesis

doi: 10.7554/eLife.56793

Figure Lengend Snippet:

Article Snippet: Antibody , USP8 (Rabbit Polyclonal) , Thermo Fisher Scientific , Cat# PA5-27947 , WB (1:1000).

Techniques: Luciferase, Knock-In, Knock-Out, Transfection, Construct, Transduction, FLAG-tag, Recombinant, Plasmid Preparation, Sequencing, Negative Control, Injection, Reporter Assay, Silver Staining, Labeling, PCR Cloning, Software, Agarose Gel Electrophoresis