Structured Review

Proteintech wnt1

Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt1 - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "SEL1L-HRD1 ER-associated degradation suppresses hepatocyte hyperproliferation and liver cancer"

Article Title: SEL1L-HRD1 ER-associated degradation suppresses hepatocyte hyperproliferation and liver cancer

Journal: iScience

doi: 10.1016/j.isci.2022.105183


Figure Legend Snippet:

Techniques Used: Western Blot, Plasmid Preparation, Microarray, Recombinant, Purification, Mutagenesis, Software, Expressing


Structured Review

Proteintech 27935 1 ap
27935 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/27935 1 ap/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
27935 1 ap - by Bioz Stars, 2024-04
93/100 stars

Images


Structured Review

Proteintech wnt 1
Abnormal expressions of SOST and <t>Wnt-1</t> as well as β-catenin in spindle type than epithelial type UM tissues. (A) Representative immunohistochemistry images for SOST and Wnt-1 as well as β-catenin in human spindle type and epithelial type UM tissues. Magnification, ×100. Scale bar, 10 μm. (B) Immunohistochemistry staining scoring of SOST and Wnt-1 as well as β-catenin levels in human spindle type (n=16) and epithelial type (n=20) UM specimens. (C) The protein-protein interactions of SOST, Wnt-1, β-catenin, LRP5, and LRP6. ****p<0.0001.
Wnt 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt 1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt 1 - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6"

Article Title: Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6

Journal: Frontiers in Oncology

doi: 10.3389/fonc.2022.898047

Abnormal expressions of SOST and Wnt-1 as well as β-catenin in spindle type than epithelial type UM tissues. (A) Representative immunohistochemistry images for SOST and Wnt-1 as well as β-catenin in human spindle type and epithelial type UM tissues. Magnification, ×100. Scale bar, 10 μm. (B) Immunohistochemistry staining scoring of SOST and Wnt-1 as well as β-catenin levels in human spindle type (n=16) and epithelial type (n=20) UM specimens. (C) The protein-protein interactions of SOST, Wnt-1, β-catenin, LRP5, and LRP6. ****p<0.0001.
Figure Legend Snippet: Abnormal expressions of SOST and Wnt-1 as well as β-catenin in spindle type than epithelial type UM tissues. (A) Representative immunohistochemistry images for SOST and Wnt-1 as well as β-catenin in human spindle type and epithelial type UM tissues. Magnification, ×100. Scale bar, 10 μm. (B) Immunohistochemistry staining scoring of SOST and Wnt-1 as well as β-catenin levels in human spindle type (n=16) and epithelial type (n=20) UM specimens. (C) The protein-protein interactions of SOST, Wnt-1, β-catenin, LRP5, and LRP6. ****p<0.0001.

Techniques Used: Immunohistochemistry, Staining

SOST knockdown activates Wnt/β-catenin signaling in UM cells. (A , B) Western blot of SOST expression in APRE-19 cells as well as three human UM cell lines OCM-1, Mum-2B, and Mum-2C. (C–H) RT-qPCR of the expressions of WNT1, CTNNB1, CCND1, MMP2, and MMP9 mRNAs in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. (I-O) Western blot of SOST, Wnt-1, β-catenin, Cyclin-D1, and MMP2 as well as MMP9 levels in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. Ns, no significance; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.
Figure Legend Snippet: SOST knockdown activates Wnt/β-catenin signaling in UM cells. (A , B) Western blot of SOST expression in APRE-19 cells as well as three human UM cell lines OCM-1, Mum-2B, and Mum-2C. (C–H) RT-qPCR of the expressions of WNT1, CTNNB1, CCND1, MMP2, and MMP9 mRNAs in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. (I-O) Western blot of SOST, Wnt-1, β-catenin, Cyclin-D1, and MMP2 as well as MMP9 levels in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. Ns, no significance; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

Techniques Used: Western Blot, Expressing, Quantitative RT-PCR, Transfection

SOST suppression activates Wnt/β-catenin pathway in ocular orthotopic tumor models. Immunohistochemistry staining for SOST, Wnt-1, β-catenin, cyclin-D1, MMP2, and MMP9 as well as Ki-67 in tumor sections from mice injected with normal, sh-NC-, or sh-SOST-transfected OCM-1 cells after six weeks. Magnification, ×40. Scale bar, 200 μm.
Figure Legend Snippet: SOST suppression activates Wnt/β-catenin pathway in ocular orthotopic tumor models. Immunohistochemistry staining for SOST, Wnt-1, β-catenin, cyclin-D1, MMP2, and MMP9 as well as Ki-67 in tumor sections from mice injected with normal, sh-NC-, or sh-SOST-transfected OCM-1 cells after six weeks. Magnification, ×40. Scale bar, 200 μm.

Techniques Used: Immunohistochemistry, Staining, Injection, Transfection


Structured Review

Proteintech anti wnt1
A Quantitative analysis of the mRNA level of Prc1 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. B PRC1 protein expression in B16 cells after silencing lncRNA-Gm31932. C Quantitative analysis of the protein level of PRC1 after silencing lncRNA-Gm31932. D Quantitative analysis of the mRNA level of Nuf2 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. E NUF2 protein expression in B16 cells after silencing lncRNA-Gm31932. F Quantitative analysis of the protein level of NUF2 after silencing lncRNA-Gm31932. G Western blotting for CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. H Quantitative analysis of the protein levels of CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. I Western blotting of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. J Quantitative analysis of the protein level of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. K Western blotting of <t>WNT1</t> and β-catenin in B16 cells after transfection with siRNA-Gm31932. L Quantitative analysis of the protein level of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. n = 3. Data are presented as the mean ± SD. ** P < 0.01 compared with the corresponding control group by one-way ANOVA followed by the Dunnett correction.
Anti Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti wnt1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti wnt1 - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/ Prc1 (and Nuf2 ) axis"

Article Title: A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/ Prc1 (and Nuf2 ) axis

Journal: Cell Death & Disease

doi: 10.1038/s41419-022-04736-6

A Quantitative analysis of the mRNA level of Prc1 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. B PRC1 protein expression in B16 cells after silencing lncRNA-Gm31932. C Quantitative analysis of the protein level of PRC1 after silencing lncRNA-Gm31932. D Quantitative analysis of the mRNA level of Nuf2 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. E NUF2 protein expression in B16 cells after silencing lncRNA-Gm31932. F Quantitative analysis of the protein level of NUF2 after silencing lncRNA-Gm31932. G Western blotting for CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. H Quantitative analysis of the protein levels of CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. I Western blotting of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. J Quantitative analysis of the protein level of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. K Western blotting of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. L Quantitative analysis of the protein level of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. n = 3. Data are presented as the mean ± SD. ** P < 0.01 compared with the corresponding control group by one-way ANOVA followed by the Dunnett correction.
Figure Legend Snippet: A Quantitative analysis of the mRNA level of Prc1 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. B PRC1 protein expression in B16 cells after silencing lncRNA-Gm31932. C Quantitative analysis of the protein level of PRC1 after silencing lncRNA-Gm31932. D Quantitative analysis of the mRNA level of Nuf2 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. E NUF2 protein expression in B16 cells after silencing lncRNA-Gm31932. F Quantitative analysis of the protein level of NUF2 after silencing lncRNA-Gm31932. G Western blotting for CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. H Quantitative analysis of the protein levels of CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. I Western blotting of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. J Quantitative analysis of the protein level of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. K Western blotting of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. L Quantitative analysis of the protein level of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. n = 3. Data are presented as the mean ± SD. ** P < 0.01 compared with the corresponding control group by one-way ANOVA followed by the Dunnett correction.

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transfection

A Representative images of tumor morphology. B Data analysis of tumor volume. C Representative IHC staining of Ki-67 expression in tumors. D Quantitative expression analysis of lncRNA-Gm31932 in tumors by RT-qPCR. E Quantitative expression analysis of miR-344d-3-5p in tumors by RT-qPCR. F Changes in the melanin content of tumors. G Changes in the tyrosinase activity of tumors. H Quantitative expression analyses of Prc1 and Nuf2 in tumors by RT-qPCR. I The protein levels of PRC1 and NUF2 in tumors were determined by western blot. J Quantitative analysis of the protein levels of PRC1 and NUF2 in tumors. K Western blotting for CDK2, CDC2, and Cyclin B1 in tumors. L Quantitative analysis of protein levels of CDK2, CDC, and Cyclin B1 in tumors. M Western blotting of P21 and P27 in tumors. N Quantitative analysis of the protein level of P21 and P27 in tumors. O Western blotting for WNT1 and β-catenin in tumors. P Quantitative analysis of the protein level of WNT1 and β-catenin in tumors. n = 8. Data are presented as the mean ± SD. * P < 0.05, ** P < 0.01 compared with the corresponding control group by Student’s t -test.
Figure Legend Snippet: A Representative images of tumor morphology. B Data analysis of tumor volume. C Representative IHC staining of Ki-67 expression in tumors. D Quantitative expression analysis of lncRNA-Gm31932 in tumors by RT-qPCR. E Quantitative expression analysis of miR-344d-3-5p in tumors by RT-qPCR. F Changes in the melanin content of tumors. G Changes in the tyrosinase activity of tumors. H Quantitative expression analyses of Prc1 and Nuf2 in tumors by RT-qPCR. I The protein levels of PRC1 and NUF2 in tumors were determined by western blot. J Quantitative analysis of the protein levels of PRC1 and NUF2 in tumors. K Western blotting for CDK2, CDC2, and Cyclin B1 in tumors. L Quantitative analysis of protein levels of CDK2, CDC, and Cyclin B1 in tumors. M Western blotting of P21 and P27 in tumors. N Quantitative analysis of the protein level of P21 and P27 in tumors. O Western blotting for WNT1 and β-catenin in tumors. P Quantitative analysis of the protein level of WNT1 and β-catenin in tumors. n = 8. Data are presented as the mean ± SD. * P < 0.05, ** P < 0.01 compared with the corresponding control group by Student’s t -test.

Techniques Used: Immunohistochemistry, Expressing, Quantitative RT-PCR, Activity Assay, Western Blot


Structured Review

Proteintech wnt1 rabbit polyclonal antibody
Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , <t>WNT1</t> (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.
Wnt1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt1 rabbit polyclonal antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt1 rabbit polyclonal antibody - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "Thymopentin-Mediated Inhibition of Cancer Stem Cell Stemness Enhances the Cytotoxic Effect of Oxaliplatin on Colon Cancer Cells"

Article Title: Thymopentin-Mediated Inhibition of Cancer Stem Cell Stemness Enhances the Cytotoxic Effect of Oxaliplatin on Colon Cancer Cells

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2022.779715

Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , WNT1 (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.
Figure Legend Snippet: Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , WNT1 (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.

Techniques Used: Western Blot, Expressing


Structured Review

Proteintech anti wnt1
Primer sequences for RT-qPCR
Anti Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti wnt1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti wnt1 - by Bioz Stars, 2024-04
93/100 stars

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1) Product Images from "MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1)"

Article Title: MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1)

Journal: Bioengineered

doi: 10.1080/21655979.2021.1977556

Primer sequences for RT-qPCR
Figure Legend Snippet: Primer sequences for RT-qPCR

Techniques Used: Sequencing

WNT1 is a direct target gene of miR-130a-3p. (a) RT-qPCR was used to detect the WNT1 mRNA expression in human CRC tissues and normal colon tissues. (b, c) Quantitation of WNT1 protein by western blot in untreated CRC cells (Control) and CRC cells with transfection of mimics control or miR-130a-3p mimics. (d, e) RT-qPCR for determining expression of WNT1 mRNA in SW480 and HCT116 cell. WNT1 expression was reduced at both protein and mRNA levels by miR-130a-3p overexpression. (f) Sequence diagrams of wild-type WNT1 3ʹ-UTR, corresponding mutant WNT1 3ʹ-UTR, and miR-130a-3p showing putative complementary binding sites. (g) Reporter assay showing that only wild-type WNT1 3ʹ-UTR suppressed luciferase activity. CRC, colorectal cancer. *P < 0.05 and **P < 0.01 vs controls
Figure Legend Snippet: WNT1 is a direct target gene of miR-130a-3p. (a) RT-qPCR was used to detect the WNT1 mRNA expression in human CRC tissues and normal colon tissues. (b, c) Quantitation of WNT1 protein by western blot in untreated CRC cells (Control) and CRC cells with transfection of mimics control or miR-130a-3p mimics. (d, e) RT-qPCR for determining expression of WNT1 mRNA in SW480 and HCT116 cell. WNT1 expression was reduced at both protein and mRNA levels by miR-130a-3p overexpression. (f) Sequence diagrams of wild-type WNT1 3ʹ-UTR, corresponding mutant WNT1 3ʹ-UTR, and miR-130a-3p showing putative complementary binding sites. (g) Reporter assay showing that only wild-type WNT1 3ʹ-UTR suppressed luciferase activity. CRC, colorectal cancer. *P < 0.05 and **P < 0.01 vs controls

Techniques Used: Quantitative RT-PCR, Expressing, Quantitation Assay, Western Blot, Transfection, Over Expression, Sequencing, Mutagenesis, Binding Assay, Reporter Assay, Luciferase, Activity Assay

WNT1 overexpression reduces the suppressive effect of miR-130a-3p on c-myc and cyclin D1 expression. (a–f) CRC cells were co-transfected with a lentivirus WNT1 overexpression vector and miR-130a-3p mimic or mimic control. RT-qPCR and Western blot revealed that enhanced WNT1 expression attenuates the downregulation of c-myc and cyclin D1 induced by miR-130a-3p. (g) Results of CRC cell colony formation assay. **P < 0.01 vs. miR-mimics + pHAGE group. a. miR-mimics; b. miR-mimics+pHAGE; c. miR-mimics + pHAGE-wnt1. CRC, colorectal cancer
Figure Legend Snippet: WNT1 overexpression reduces the suppressive effect of miR-130a-3p on c-myc and cyclin D1 expression. (a–f) CRC cells were co-transfected with a lentivirus WNT1 overexpression vector and miR-130a-3p mimic or mimic control. RT-qPCR and Western blot revealed that enhanced WNT1 expression attenuates the downregulation of c-myc and cyclin D1 induced by miR-130a-3p. (g) Results of CRC cell colony formation assay. **P < 0.01 vs. miR-mimics + pHAGE group. a. miR-mimics; b. miR-mimics+pHAGE; c. miR-mimics + pHAGE-wnt1. CRC, colorectal cancer

Techniques Used: Over Expression, Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Colony Assay

Overexpressed miR-130a-3p inhibits colorectal cancer cell growth in a mouse xenograft model. (a-c) The total weights and volumes of tumors derived from the indicated CRC cell line were decreased by miR-130a-3p overexpression (mimics group) compared with control CRC cell lines. (d) RT-qPCR results showed WNT1, Cyclin D1 and c-myc mRNA expression was downregulated in mimics group, compared with control and mimics control groups. **P < 0.01. Per group n = 6
Figure Legend Snippet: Overexpressed miR-130a-3p inhibits colorectal cancer cell growth in a mouse xenograft model. (a-c) The total weights and volumes of tumors derived from the indicated CRC cell line were decreased by miR-130a-3p overexpression (mimics group) compared with control CRC cell lines. (d) RT-qPCR results showed WNT1, Cyclin D1 and c-myc mRNA expression was downregulated in mimics group, compared with control and mimics control groups. **P < 0.01. Per group n = 6

Techniques Used: Derivative Assay, Over Expression, Quantitative RT-PCR, Expressing


Structured Review

Proteintech primary antibodies against wnt 1
Primary Antibodies Against Wnt 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against wnt 1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
primary antibodies against wnt 1 - by Bioz Stars, 2024-04
93/100 stars

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Structured Review

Proteintech anti wnt1
Anti Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti wnt1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti wnt1 - by Bioz Stars, 2024-04
93/100 stars

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Structured Review

Proteintech wnt1
Hyperin blocked wnt/β-catenin signal pathway. ( A ) The TOP/FOP luciferase activity was detected in AGS and MKN-45 cells. n=5, * P <0.05. ( B ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and <t>wnt1</t> in AGS and MKN-45 cells. n=5, * P <0.05.
Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt1 - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "Hyperin Controls the Development and Therapy of Gastric Cancer via Regulating Wnt/β-Catenin Signaling"

Article Title: Hyperin Controls the Development and Therapy of Gastric Cancer via Regulating Wnt/β-Catenin Signaling

Journal: Cancer Management and Research

doi: 10.2147/CMAR.S270544

Hyperin blocked wnt/β-catenin signal pathway. ( A ) The TOP/FOP luciferase activity was detected in AGS and MKN-45 cells. n=5, * P <0.05. ( B ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in AGS and MKN-45 cells. n=5, * P <0.05.
Figure Legend Snippet: Hyperin blocked wnt/β-catenin signal pathway. ( A ) The TOP/FOP luciferase activity was detected in AGS and MKN-45 cells. n=5, * P <0.05. ( B ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in AGS and MKN-45 cells. n=5, * P <0.05.

Techniques Used: Luciferase, Activity Assay

Hyperin significantly reduces tumor growth in vivo. ( A ) Tumors removed from the mice 30 days after Hyperin and Control treated, respectively n=5. ( B and C ) Tumor volume and weight were shown after the tumors were collected. n=5, * P <0.05. ( D ) H&E staining and TUNEL assay were performed. ( E ) Representative image of ki-67, caspase3, wnt1, and β-catenin IHC staining. ( F ) The protein level of Bcl2, Bax, cleaved-caspase3 in tumor tissues. n=5, * P <0.05. ( G ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in tumor tissues. n=5, * P <0.05.
Figure Legend Snippet: Hyperin significantly reduces tumor growth in vivo. ( A ) Tumors removed from the mice 30 days after Hyperin and Control treated, respectively n=5. ( B and C ) Tumor volume and weight were shown after the tumors were collected. n=5, * P <0.05. ( D ) H&E staining and TUNEL assay were performed. ( E ) Representative image of ki-67, caspase3, wnt1, and β-catenin IHC staining. ( F ) The protein level of Bcl2, Bax, cleaved-caspase3 in tumor tissues. n=5, * P <0.05. ( G ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in tumor tissues. n=5, * P <0.05.

Techniques Used: In Vivo, Staining, TUNEL Assay, Immunohistochemistry


Structured Review

Proteintech wnt1
Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt1 - by Bioz Stars, 2024-04
93/100 stars

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    Proteintech wnt1

    Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wnt1 - by Bioz Stars, 2024-04
    93/100 stars
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    93
    Proteintech 27935 1 ap

    27935 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/27935 1 ap/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Proteintech wnt 1
    Abnormal expressions of SOST and <t>Wnt-1</t> as well as β-catenin in spindle type than epithelial type UM tissues. (A) Representative immunohistochemistry images for SOST and Wnt-1 as well as β-catenin in human spindle type and epithelial type UM tissues. Magnification, ×100. Scale bar, 10 μm. (B) Immunohistochemistry staining scoring of SOST and Wnt-1 as well as β-catenin levels in human spindle type (n=16) and epithelial type (n=20) UM specimens. (C) The protein-protein interactions of SOST, Wnt-1, β-catenin, LRP5, and LRP6. ****p<0.0001.
    Wnt 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt 1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wnt 1 - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    93
    Proteintech anti wnt1
    A Quantitative analysis of the mRNA level of Prc1 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. B PRC1 protein expression in B16 cells after silencing lncRNA-Gm31932. C Quantitative analysis of the protein level of PRC1 after silencing lncRNA-Gm31932. D Quantitative analysis of the mRNA level of Nuf2 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. E NUF2 protein expression in B16 cells after silencing lncRNA-Gm31932. F Quantitative analysis of the protein level of NUF2 after silencing lncRNA-Gm31932. G Western blotting for CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. H Quantitative analysis of the protein levels of CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. I Western blotting of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. J Quantitative analysis of the protein level of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. K Western blotting of <t>WNT1</t> and β-catenin in B16 cells after transfection with siRNA-Gm31932. L Quantitative analysis of the protein level of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. n = 3. Data are presented as the mean ± SD. ** P < 0.01 compared with the corresponding control group by one-way ANOVA followed by the Dunnett correction.
    Anti Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech wnt1 rabbit polyclonal antibody
    Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , <t>WNT1</t> (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.
    Wnt1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt1 rabbit polyclonal antibody/product/Proteintech
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    Proteintech primary antibodies against wnt 1
    Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , <t>WNT1</t> (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.
    Primary Antibodies Against Wnt 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against wnt 1/product/Proteintech
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    Image Search Results


    Journal: iScience

    Article Title: SEL1L-HRD1 ER-associated degradation suppresses hepatocyte hyperproliferation and liver cancer

    doi: 10.1016/j.isci.2022.105183

    Figure Lengend Snippet:

    Article Snippet: The following primary antibodies were used: KI67 (1:200, Abcam ab16667); CYCLIN D1 (1:100 for staining, 1:2000 for Western blot, Abcam ab16663); c-MYC (1:1000, MilliporeSigma C3956); V5 (1:5000 for Western blot, 1:250 for immunoprecipitation, Invitrogen R960-25); HA (1:3,000, Sigma H9658); WNT5A (1:2000 for Western blot, 1:100 for immunoprecipitation, Proteintech 55184-1-AP); WNT5B (1:1000, Abclonal A8313); WNT1 (1:1000, Proteintech 27935-1-AP); E-CADHERIN (1:1000, BD 610181); α-TUBULIN (1:2,000, Santa Cruz sc-5286); HSP90 (1:1,000, Abcam ab13492); CALNEXIN (1:5000, Enzo ADI-SPA-860-F); SEL1L (1:2,000, Abcam ab78298); HRD1 (1:300, kindly gifted by Dr. Richard Wojcikiewicz, SUNY Upstate Medical University for Western blot; 1:1000, Sigma HPA024300 for immunostaining of human liver tumors).

    Techniques: Western Blot, Plasmid Preparation, Microarray, Recombinant, Purification, Mutagenesis, Software, Expressing

    Abnormal expressions of SOST and Wnt-1 as well as β-catenin in spindle type than epithelial type UM tissues. (A) Representative immunohistochemistry images for SOST and Wnt-1 as well as β-catenin in human spindle type and epithelial type UM tissues. Magnification, ×100. Scale bar, 10 μm. (B) Immunohistochemistry staining scoring of SOST and Wnt-1 as well as β-catenin levels in human spindle type (n=16) and epithelial type (n=20) UM specimens. (C) The protein-protein interactions of SOST, Wnt-1, β-catenin, LRP5, and LRP6. ****p<0.0001.

    Journal: Frontiers in Oncology

    Article Title: Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6

    doi: 10.3389/fonc.2022.898047

    Figure Lengend Snippet: Abnormal expressions of SOST and Wnt-1 as well as β-catenin in spindle type than epithelial type UM tissues. (A) Representative immunohistochemistry images for SOST and Wnt-1 as well as β-catenin in human spindle type and epithelial type UM tissues. Magnification, ×100. Scale bar, 10 μm. (B) Immunohistochemistry staining scoring of SOST and Wnt-1 as well as β-catenin levels in human spindle type (n=16) and epithelial type (n=20) UM specimens. (C) The protein-protein interactions of SOST, Wnt-1, β-catenin, LRP5, and LRP6. ****p<0.0001.

    Article Snippet: The membrane was sealed with 5% nonfat dry milk and then incubated with primary antibodies against SOST (1:1000; 21933-1-AP; Proteintech, China), Wnt-1 (1:1000; 27935-1-AP), β-catenin (1:5000; 51067-2-AP), cyclin-D1 (1:2000; 26939-1-AP), MMP2 (1:500; 10373-2-AP), and MMP9 (1:500; 10375-2-AP) as well as β-Tubulin (1:1000; 10094-1-AP) at 4°C overnight.

    Techniques: Immunohistochemistry, Staining

    SOST knockdown activates Wnt/β-catenin signaling in UM cells. (A , B) Western blot of SOST expression in APRE-19 cells as well as three human UM cell lines OCM-1, Mum-2B, and Mum-2C. (C–H) RT-qPCR of the expressions of WNT1, CTNNB1, CCND1, MMP2, and MMP9 mRNAs in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. (I-O) Western blot of SOST, Wnt-1, β-catenin, Cyclin-D1, and MMP2 as well as MMP9 levels in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. Ns, no significance; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

    Journal: Frontiers in Oncology

    Article Title: Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6

    doi: 10.3389/fonc.2022.898047

    Figure Lengend Snippet: SOST knockdown activates Wnt/β-catenin signaling in UM cells. (A , B) Western blot of SOST expression in APRE-19 cells as well as three human UM cell lines OCM-1, Mum-2B, and Mum-2C. (C–H) RT-qPCR of the expressions of WNT1, CTNNB1, CCND1, MMP2, and MMP9 mRNAs in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. (I-O) Western blot of SOST, Wnt-1, β-catenin, Cyclin-D1, and MMP2 as well as MMP9 levels in si-SOST-transfected OCM-1, Mum-2B, and Mum-2C cells and controls. Ns, no significance; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

    Article Snippet: The membrane was sealed with 5% nonfat dry milk and then incubated with primary antibodies against SOST (1:1000; 21933-1-AP; Proteintech, China), Wnt-1 (1:1000; 27935-1-AP), β-catenin (1:5000; 51067-2-AP), cyclin-D1 (1:2000; 26939-1-AP), MMP2 (1:500; 10373-2-AP), and MMP9 (1:500; 10375-2-AP) as well as β-Tubulin (1:1000; 10094-1-AP) at 4°C overnight.

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Transfection

    SOST suppression activates Wnt/β-catenin pathway in ocular orthotopic tumor models. Immunohistochemistry staining for SOST, Wnt-1, β-catenin, cyclin-D1, MMP2, and MMP9 as well as Ki-67 in tumor sections from mice injected with normal, sh-NC-, or sh-SOST-transfected OCM-1 cells after six weeks. Magnification, ×40. Scale bar, 200 μm.

    Journal: Frontiers in Oncology

    Article Title: Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6

    doi: 10.3389/fonc.2022.898047

    Figure Lengend Snippet: SOST suppression activates Wnt/β-catenin pathway in ocular orthotopic tumor models. Immunohistochemistry staining for SOST, Wnt-1, β-catenin, cyclin-D1, MMP2, and MMP9 as well as Ki-67 in tumor sections from mice injected with normal, sh-NC-, or sh-SOST-transfected OCM-1 cells after six weeks. Magnification, ×40. Scale bar, 200 μm.

    Article Snippet: The membrane was sealed with 5% nonfat dry milk and then incubated with primary antibodies against SOST (1:1000; 21933-1-AP; Proteintech, China), Wnt-1 (1:1000; 27935-1-AP), β-catenin (1:5000; 51067-2-AP), cyclin-D1 (1:2000; 26939-1-AP), MMP2 (1:500; 10373-2-AP), and MMP9 (1:500; 10375-2-AP) as well as β-Tubulin (1:1000; 10094-1-AP) at 4°C overnight.

    Techniques: Immunohistochemistry, Staining, Injection, Transfection

    A Quantitative analysis of the mRNA level of Prc1 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. B PRC1 protein expression in B16 cells after silencing lncRNA-Gm31932. C Quantitative analysis of the protein level of PRC1 after silencing lncRNA-Gm31932. D Quantitative analysis of the mRNA level of Nuf2 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. E NUF2 protein expression in B16 cells after silencing lncRNA-Gm31932. F Quantitative analysis of the protein level of NUF2 after silencing lncRNA-Gm31932. G Western blotting for CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. H Quantitative analysis of the protein levels of CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. I Western blotting of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. J Quantitative analysis of the protein level of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. K Western blotting of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. L Quantitative analysis of the protein level of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. n = 3. Data are presented as the mean ± SD. ** P < 0.01 compared with the corresponding control group by one-way ANOVA followed by the Dunnett correction.

    Journal: Cell Death & Disease

    Article Title: A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/ Prc1 (and Nuf2 ) axis

    doi: 10.1038/s41419-022-04736-6

    Figure Lengend Snippet: A Quantitative analysis of the mRNA level of Prc1 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. B PRC1 protein expression in B16 cells after silencing lncRNA-Gm31932. C Quantitative analysis of the protein level of PRC1 after silencing lncRNA-Gm31932. D Quantitative analysis of the mRNA level of Nuf2 in B16 cells after silencing lncRNA-Gm31932 by RT-qPCR. E NUF2 protein expression in B16 cells after silencing lncRNA-Gm31932. F Quantitative analysis of the protein level of NUF2 after silencing lncRNA-Gm31932. G Western blotting for CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. H Quantitative analysis of the protein levels of CDK2, CDC2, and Cyclin B1 in B16 cells after transfection with siRNA-Gm31932-174. I Western blotting of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. J Quantitative analysis of the protein level of P21 and P27 in B16 cells after transfection with siRNA-Gm31932-174. K Western blotting of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. L Quantitative analysis of the protein level of WNT1 and β-catenin in B16 cells after transfection with siRNA-Gm31932. n = 3. Data are presented as the mean ± SD. ** P < 0.01 compared with the corresponding control group by one-way ANOVA followed by the Dunnett correction.

    Article Snippet: Then, the membrane was incubated in 5% bovine serum albumin (BSA) in TBST buffer at room temperature for 2 h, followed by incubation at 4 °C overnight with the primary antibodies as follows: anti-β-actin (1:5000; ab179467, Abcam), anti-CDC2 (1:1000; ab201008, Abcam), anti-CDK2 (1:1000; ab32147, Abcam), anti-Cyclin B1 (1:2000; ab181593, Abcam), anti-P21 (1:1000; ab188224, Abcam), anti-P27 (1:2000; ab193379, Abcam), anti-Wnt1 (1:500; 27935-1-AP, Proteintech), anti-β-catenin (1:500; ab68183, Abcam), anti-PRC1 (1:5000; ab51248, Abcam), and anti-NUF2 (1:1000; ab230313, Abcam).

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Transfection

    A Representative images of tumor morphology. B Data analysis of tumor volume. C Representative IHC staining of Ki-67 expression in tumors. D Quantitative expression analysis of lncRNA-Gm31932 in tumors by RT-qPCR. E Quantitative expression analysis of miR-344d-3-5p in tumors by RT-qPCR. F Changes in the melanin content of tumors. G Changes in the tyrosinase activity of tumors. H Quantitative expression analyses of Prc1 and Nuf2 in tumors by RT-qPCR. I The protein levels of PRC1 and NUF2 in tumors were determined by western blot. J Quantitative analysis of the protein levels of PRC1 and NUF2 in tumors. K Western blotting for CDK2, CDC2, and Cyclin B1 in tumors. L Quantitative analysis of protein levels of CDK2, CDC, and Cyclin B1 in tumors. M Western blotting of P21 and P27 in tumors. N Quantitative analysis of the protein level of P21 and P27 in tumors. O Western blotting for WNT1 and β-catenin in tumors. P Quantitative analysis of the protein level of WNT1 and β-catenin in tumors. n = 8. Data are presented as the mean ± SD. * P < 0.05, ** P < 0.01 compared with the corresponding control group by Student’s t -test.

    Journal: Cell Death & Disease

    Article Title: A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/ Prc1 (and Nuf2 ) axis

    doi: 10.1038/s41419-022-04736-6

    Figure Lengend Snippet: A Representative images of tumor morphology. B Data analysis of tumor volume. C Representative IHC staining of Ki-67 expression in tumors. D Quantitative expression analysis of lncRNA-Gm31932 in tumors by RT-qPCR. E Quantitative expression analysis of miR-344d-3-5p in tumors by RT-qPCR. F Changes in the melanin content of tumors. G Changes in the tyrosinase activity of tumors. H Quantitative expression analyses of Prc1 and Nuf2 in tumors by RT-qPCR. I The protein levels of PRC1 and NUF2 in tumors were determined by western blot. J Quantitative analysis of the protein levels of PRC1 and NUF2 in tumors. K Western blotting for CDK2, CDC2, and Cyclin B1 in tumors. L Quantitative analysis of protein levels of CDK2, CDC, and Cyclin B1 in tumors. M Western blotting of P21 and P27 in tumors. N Quantitative analysis of the protein level of P21 and P27 in tumors. O Western blotting for WNT1 and β-catenin in tumors. P Quantitative analysis of the protein level of WNT1 and β-catenin in tumors. n = 8. Data are presented as the mean ± SD. * P < 0.05, ** P < 0.01 compared with the corresponding control group by Student’s t -test.

    Article Snippet: Then, the membrane was incubated in 5% bovine serum albumin (BSA) in TBST buffer at room temperature for 2 h, followed by incubation at 4 °C overnight with the primary antibodies as follows: anti-β-actin (1:5000; ab179467, Abcam), anti-CDC2 (1:1000; ab201008, Abcam), anti-CDK2 (1:1000; ab32147, Abcam), anti-Cyclin B1 (1:2000; ab181593, Abcam), anti-P21 (1:1000; ab188224, Abcam), anti-P27 (1:2000; ab193379, Abcam), anti-Wnt1 (1:500; 27935-1-AP, Proteintech), anti-β-catenin (1:500; ab68183, Abcam), anti-PRC1 (1:5000; ab51248, Abcam), and anti-NUF2 (1:1000; ab230313, Abcam).

    Techniques: Immunohistochemistry, Expressing, Quantitative RT-PCR, Activity Assay, Western Blot

    Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , WNT1 (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.

    Journal: Frontiers in Pharmacology

    Article Title: Thymopentin-Mediated Inhibition of Cancer Stem Cell Stemness Enhances the Cytotoxic Effect of Oxaliplatin on Colon Cancer Cells

    doi: 10.3389/fphar.2022.779715

    Figure Lengend Snippet: Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , WNT1 (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.

    Article Snippet: The membranes were incubated overnight at 4°C with primary antibody, and secondary antibodies anti-rabbit IgG HRP-conjugated (1: 3,000, 7074S, Cell Signaling Technology) and anti-mouse IgG HRP-conjugated (1: 3,000, 7076S, Cell Signaling Technology) were incubated at 25°C for incubate for 2 h. The primary antibodies used are Beta Catenin Rabbit Polyclonal Antibody (1: 1,000, 51067-2-AP, Proteintech), WNT1 Rabbit Polyclonal Antibody (1: 1,000, 27935-1-AP, Proteintech), Phospho-beta Catenin (Ser33/37/Thr41) antibody (1: 1,000, 9561, Cell Signaling Technology), AKT1 (Phospho-Ser473 + Tyr474) antibody (1: 1,000, 12669, Abcam), PI3K p110 (beta) polyclonal antibody (1: 1,000, 20584-1-AP, Proteintech), AKT1 polyclonal antibody (1: 1,000, 10176-2-AP, Proteintech), and GAPDH (1: 10,000, 60004-I-Ig, Proteintech).

    Techniques: Western Blot, Expressing