Journal: International Dental Journal

Article Title: Probiotic Bifidobacteria in Managing Periodontal Disease: A Systematic Review

doi: 10.1016/j.identj.2022.11.018

Figure Lengend Snippet: Characteristics of the included studies on genus Bifidobacterium probiotics used in chronic periodontitis cases in RCTs.

Article Snippet: Argandoña Valdez et al (2021) Brazil , PROB strains: Bifidobacterium longum subspecies longum (ATCC 15,707), Bifidobacterium longum subspecies infantis (ATCC 15,697), and Bifidobacterium animalis subspecies lactis (ATCC 27,673) vs periodontal pathogens: Fusobacterium nucleatum subspecies nucleatum (ATCC 25,585), Porphyromonas gingivalis (33,277), and Streptococcus oralis were grown in brain heart infusion agar , Bacterial strains were incubated for 24 h, 72 h, and 168 h at 37 °C , Checkerboards DNA–DNA hybridisation , The absolute counts of each bacterium in combination were presented in the percentage of bacterial growth.

Techniques:

Journal: International Dental Journal

Article Title: Probiotic Bifidobacteria in Managing Periodontal Disease: A Systematic Review

doi: 10.1016/j.identj.2022.11.018

Figure Lengend Snippet: Characteristics of the included in vitro and animal model studies examining genus Bifidobacterium probiotics.

Article Snippet: Argandoña Valdez et al (2021) Brazil , PROB strains: Bifidobacterium longum subspecies longum (ATCC 15,707), Bifidobacterium longum subspecies infantis (ATCC 15,697), and Bifidobacterium animalis subspecies lactis (ATCC 27,673) vs periodontal pathogens: Fusobacterium nucleatum subspecies nucleatum (ATCC 25,585), Porphyromonas gingivalis (33,277), and Streptococcus oralis were grown in brain heart infusion agar , Bacterial strains were incubated for 24 h, 72 h, and 168 h at 37 °C , Checkerboards DNA–DNA hybridisation , The absolute counts of each bacterium in combination were presented in the percentage of bacterial growth.

Techniques: In Vitro, Animal Model, Incubation, DNA-DNA Hybridization, Inhibition, Cell Culture, Immunohistochemical staining, Micro-CT

Journal: International Dental Journal

Article Title: Probiotic Bifidobacteria in Managing Periodontal Disease: A Systematic Review

doi: 10.1016/j.identj.2022.11.018

Figure Lengend Snippet: Results of the included studies.

Article Snippet: Argandoña Valdez et al (2021) Brazil , PROB strains: Bifidobacterium longum subspecies longum (ATCC 15,707), Bifidobacterium longum subspecies infantis (ATCC 15,697), and Bifidobacterium animalis subspecies lactis (ATCC 27,673) vs periodontal pathogens: Fusobacterium nucleatum subspecies nucleatum (ATCC 25,585), Porphyromonas gingivalis (33,277), and Streptococcus oralis were grown in brain heart infusion agar , Bacterial strains were incubated for 24 h, 72 h, and 168 h at 37 °C , Checkerboards DNA–DNA hybridisation , The absolute counts of each bacterium in combination were presented in the percentage of bacterial growth.

Techniques: Concentration Assay, In Vitro, Activity Assay, Inhibition, Animal Model, Expressing

Journal: eLife

Article Title: Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation

doi: 10.7554/eLife.78929

Figure Lengend Snippet: Representative confocal micrographs of HeLa cells expressing dark MitoTrap and mCherry- Epsin-2 ( A ) or mCherry-Fcho2 ( E ) (red) with either clathrin hook (FKBP-β2-GFP) or GFP-FKBP (green). Cells were treated with 200 nM rapamycin before staining for PDHE2/E3 (blue). Inset, ×5 zoom. Scale bars, 10 μm. Box plots to compare colocalization ( B, F ) and spot density ( C, G ). Each dot represents a cell. Box plots indicate median, IQR, 9th and 91st percentiles. Spots of GFP and mCherry were detected and quantified. Colocalization is shown as the percentage of GFP spots that coincided with Epsin-2 or Fcho2 spots (left), or the percentage of Epsin-2 or Fcho2 spots that coincided with GFP spots (right). ( D, H ) Waffle plots to visualize the median number of spots per 100 μm 2 that were positive for GFP only (green), clathrin only (red), or both (yellow).

Article Snippet: Plasmids for mCherry-Epsin-2 (#27673), mCherry-Fcho2 (#27686), Hip1r-tDimer-RFP (#27700), mCherry-Amphiphysin (#27692), and mCherry-Snx9 (#27678) (all mouse) were from Addgene.

Techniques: Expressing, Staining

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Probiotic Species in the Management of Periodontal Diseases: An Overview

doi: 10.3389/fcimb.2022.806463

Figure Lengend Snippet: The regulatory effects of known probiotic species promoting periodontal health.

Article Snippet: In in vitro experiments, B. lactis ATCC 27673 antagonized the biofilm formation of F. nucleatum ATCC 25585 and P. gingivalis ATCC 33277 after co-incubating for 168 h, without interfering with the growth of Streptococcus oralis ( ).

Techniques: In Vitro, Infection, Expressing, Lysis, Activation Assay, Recombinant, Activity Assay