Journal: Life

Article Title: Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?

doi: 10.3390/life12101492

Figure Lengend Snippet: MLSA resultant phylogenetic tree, using six housekeeping genes: recA , gyrB , gltA , metG , groL , and 16S rRNA and 28 genomes from Aeromonas genus, inferred with Maximum Likelihood (ML) method and General Time Reversible model. The model evolutionary used was a discrete Gamma distribution. The bootstrap consensus was inferred from 1000 replicates. Oceanimonas doudoroffi ATCC 27123 was used as an outgroup.

Article Snippet: Oceanimonas doudoroffi ATCC 27123 was used as an outgroup.

Techniques:

Journal: bioRxiv

Article Title: Bright, fluorogenic and photostable avidity probes for RNA imaging

doi: 10.1101/2021.11.02.466936

Figure Lengend Snippet: a) Confocal images of fixed MEF cells expressing GFP -β- actin-biRhoBAST 8 mRNA after incubation with smFISH probes (targeting the GFP sequence) in the presence of TMR 2 (50 nM). The overlay image shows significant colocalization of smFISH and TMR signals. Scale bar, 5 µm. b) Confocal images of live Cos7 cells co-transfected with P CMV - mAzurite-biRhoBAST 8 -MS2 24 and P UbC -NLS-stdMCP-stdGFP plasmids in the presence of 50 nM TMR 2 . The overlay image shows significant co-localization of GFP and TMR signals. Scale bar, 10 µm. c) Spinning disc confocal images of Cos7 cells expressing low levels of MCP-GFP and mAzurite-biRhoBAST 8 -MS2 24 (top), mAzurite-biRhoBAST 8 (middle), mAzurite-MS2 24 (bottom) in the presence of 500 nM TMR 2 . Single mRNA molecules were visualized with a frame rate of 10 s −1 . Scale bars, 10 µm. d) Extracted trajectories at different time points of single RNA tracks in the image shown in panel c (top), revealing synchronous movements of TMR 2 and GFP signals over time. e) Colocalization test of the cytosolic TMR 2 and GFP signals in the image shown in panel c (top), resulting in a Pearson’s R value of 0.83.

Article Snippet: To yield GFP-β-actin-Zip-biRhoBAST n , XbaI/NheI-digested biRhoBAST n cassettes were ligated into XbaI-digested and dephosphorylated eTC-GFP-β-actin-Zip (Addgene Plasmid #27123).

Techniques: Expressing, Incubation, Sequencing, Transfection

Journal: eLife

Article Title: Different translation dynamics of β- and γ-actin regulates cell migration

doi: 10.7554/eLife.68712

Figure Lengend Snippet:

Article Snippet: Constructs were generated using the eTC GFP beta-actin full-length plasmid (Addgene plasmid # 27123) and eTC GFP beta-actin ΔZip (Addgene plasmid # 27124), which were gifts from Robert Singer ( ).

Techniques: Derivative Assay, Cell Culture, Transfection, Construct, Plasmid Preparation, Transduction, Recombinant, Sequencing, Clone Assay, Software