p kunmingensis dsm 25974 t  (ATCC)

Journal: Frontiers in Pharmacology

Article Title: Baicalin Ameliorates Pancreatic Fibrosis by Inhibiting the Activation of Pancreatic Stellate Cells in Mice with Chronic Pancreatitis

doi: 10.3389/fphar.2020.607133

Figure Lengend Snippet: Baicalin protects against pancreatic injury and pancreatic fibrosis in mice with chronic pancreatitis (CP) induced by cerulein. (A) Changes in pancreatic morphology at different timepoints were evaluated by hematoxylin and eosin staining (original magnification, ×200). (B) Collagen deposition was detected by Masson staining (original magnification, ×200). (C) Immunohistochemistry of COL1A1was performed to evaluate the degree of pancreatic fibrosis, and the brown color shows positively stained cells. (original magnification, ×400). (D) Protein expression of COL1A1 in pancreas was analyzed by Western blot, and GAPDH was used as an internal control; the relative protein abundance was quantified by densitometry analysis. Values are shown as mean ± SD ( n = 3). ** p < 0.01 vs. control; ## p < 0.01 vs CP group.

Article Snippet: The sections (3 μm) were dewaxed with water, boiled with citric acid buffer for antigen repair, and blocked with 3% H 2 O 2 for 10 min and 5% bovine serum albumin (BSA) for 40 min. Antibodies were applied overnight at 4°C at the following concentrations: anti-F4/80 antibody (sc-71088, Santa Cruz, 1:200), anti-MCP-1 antibody (bs-1101R, Bioss, 1:200), anti-NF-κB p65 antibody (sc-8008, Santa Cruz, 1:200), anti-α-SMA antibody (bs-10196R, Bioss, 1:200), and anti-COL1A1 antibody (sc-25974, Santa Cruz, 1:200).

Techniques: Staining, Immunohistochemistry, Expressing, Western Blot, Control, Quantitative Proteomics