Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: The characteristics of primer sets and PCR conditions used to detect mRNA expression.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing, Sequencing

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: Expression of AQP5, UT-A1, and UT-B1 in skin tissue by immunohistochemistry. (a1), (c1) (×40); (b1), (b2), (b3), (b4) (×200); (a2), (c2), (a3), (c3), (a4), (c4), (a5), (b5), (c5) (×400). FS : full-thickness skin; PC : positive control; UP : uremic patients; NMC : normal controls; NGC : negative control.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing, Immunohistochemistry, Positive Control, Negative Control

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: Semiquantitative analysis of the expression of AQP5, UT-A1, and UT-B1. Comparison of AQP5, UT-A1, and UT-B1 protein expression in each group. ∗ P < 0.01; # P < 0.05.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: Expression of AQP5, UT-A1, and UT-B1 mRNA in human skin. ∗ P < 0.01; # P < 0.05.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: The relative expression of AQP5, UT-A1, and UT-B1 protein in human skin. ∗ P < 0.01; # P < 0.05.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Type strains of Rubrobacter species and those of related genera, their growth requirements and 16S rRNA gene sequence accession numbers

Article Snippet: Patulibacter medicamentivorans , DSM 25962 T , DSMZ medium No. 830 , 28 , AGUD01000068 , Almeida et al. ( ) .

Techniques: Sequencing

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Conserved nucleotide regions of 16S rRNA genes of Rubrobacter type strains used to design the specific primers Rubro223f and Rubro454r. The bar represents the 16S rRNA gene sequence of Escherichia coli ; black boxes indicate conserved regions and the grey ones variable regions (V) with corresponding numbers (Brosius et al. ; Yarza et al. ). Arrows above the bar represent the position of the primers within the 16S rRNA gene sequence. Nucleotide alignment for primers Rubro223f and Rubro454r and for primer Rubro749r (Holmes et al. ) are highlighted in orange boxes and nucleotides in white represent those unique to the genus Rubrobacter and hence absent in the type strains of species classified in the genera Conexibacter , Gaiella , Parviterribacter , Patulibacter, Solirubrobacter and Thermoleophilum . (Color figure online)

Article Snippet: Patulibacter medicamentivorans , DSM 25962 T , DSMZ medium No. 830 , 28 , AGUD01000068 , Almeida et al. ( ) .

Techniques: Sequencing

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Maximum-likelihood phylogenetic tree generated using the GTR + CAT model and rooted by midpoint-rooting showing relationships between the 267 nt sequences amplified with primers Rubro223f and Rubro454r, using community DNA extracted from Salar de Tara (ST1) and Quebrada Nacimiento (QN) soils and the corresponding full 16S rRNA gene sequences of the type strains of representatives of the genera Conexibacter , Gaiella , Parviterribacter , Patulibacter, Solirubrobacter and Thermoleophilum . The branches of the tree are scaled in terms of the expected number of substitutions per site and the numbers above the branches are bootstrap support values greater than 60% for the ML (left) and MP (right) analyses

Article Snippet: Patulibacter medicamentivorans , DSM 25962 T , DSMZ medium No. 830 , 28 , AGUD01000068 , Almeida et al. ( ) .

Techniques: Generated, Amplification

Journal: FEMS Microbiology Ecology

Article Title: Identification of phenol- and p -cresol-producing intestinal bacteria by using media supplemented with tyrosine and its metabolites

doi: 10.1093/femsec/fiy125

Figure Lengend Snippet: Bacterial strains used in this study, and culture conditions

Article Snippet: 24 , Bifidobacterium longum subsp. infantis , YIT 4018 T = ATCC 15697 T , modified PYG broth.

Techniques: Modification

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Bacterial growth while utilizing cranberry xyloglucans. Growth curves of B. longum subsp. longum UCD401, L. plantarum ATCC BAA-793, L. johnsonii ATCC 33200, and B. longum subsp. infantis ATCC 15697 and JCM 1260 grown on mMRS medium containing 2% (wt/vol) xyloglucans. The curves are drawn from an average of three independent experiments.

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Analysis of bacterial growth kinetics calculated with Wolfram Mathematica 10.3

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Bacterial fermentative end products of cranberry xyloglucan utilization. Shown are lactate (a), acetate (b), and formate (c) production; acetate/lactate ratios (d); and formate/lactate ratios (e). Bl, B. longum subsp. longum UCD401; Lp, Lactobacillus plantarum ATCC BAA-793. Averages of independent biological triplicates are shown, and bars represent the standard deviation of the mean. Organic acid production is expressed in millimolar absolute concentrations. Asterisks represent significant differences determined by two-way ANOVA and Sidak's multiple-comparison test (P < 0.05).

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques: Standard Deviation

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Bacterial growth on additional fractions of cranberry xyloglucans. Growth curves represent B. longum subsp. infantis ATCC 15697, JCM 1260, JCM 1272, and JCM 7007; B. longum subsp. longum JCM 11347, ATCC 15708, and UCD401; Lactobacillus plantarum ATCC BAA-793; and Lactobacillus johnsonii ATCC 33200 grown on mMRS medium containing 2% (wt/vol) xyloglucan fraction A2 (a) or 2% (wt/vol) xyloglucan fraction A6 (b). The curves are drawn from the average of at least three independent experiments, with the exception of strains JCM 1260, JCM 7007, JCM 11347, and JCM 15708 which are based on duplicates in panel a.

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Kinetic analysis of bacterial growth on A2 and A6 fraction xyloglucans calculated with Wolfram Mathematica 10.3

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Bifidobacterial fermentative end products of cranberry fraction A6 utilization. (a to c) Lactate (a), acetate (b), and formate (c) production while B. longum subsp. infantis JCM 1260 and B. longum subsp. longum UCD401 utilize xyloglucan fraction A6. (d) Acetate:lactate ratio after fermentation. Averages of independent biological triplicates are shown, and bars represent the standard deviation of the mean. Asterisks represent significant differences evaluated by two-way ANOVA and Sidak's multiple-comparison test (P < 0.05).

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques: Standard Deviation

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Gene expression of B. longum subsp. longum UCD401 while utilizing cranberry fraction A6 as a sole carbon source. Genes in the arabinose cluster of B. longum subsp. longum UCD401 predicted to participate in xyloglucan metabolism are depicted on the x axis. Expression of four GH family genes (those for α-l-arabinofuranosidase, C-terminal [BL_0405], arabinan endo-1,5-α-l-arabinosidase [BL_0404, BL_0403], β-xylosidase [BL_0402]) (a) and those for a carbohydrate ABC transporter substrate-binding protein (BL_0398) and carbohydrate ABC transporter membrane proteins (BL_0397, BL_0396) (b) is shown as fold change relative to the control (glucose). Averages of independent biological triplicates are shown, and bars represent the standard deviation of the mean. Asterisks (* and **) represent significant differences compared to the control (glucose), evaluated by paired t test (P values of <0.05 and <0.005, respectively).

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques: Expressing, Binding Assay, Standard Deviation

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Growth kinetics of strains during syntrophic interaction

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

doi: 10.1128/AEM.01097-17

Figure Lengend Snippet: Bacterial syntrophic interactions modeled with cranberry xyloglucans. Growth curves of B. longum subsp. infantis ATCC 15697 on mMRS medium containing xyloglucans (green) and supernatants from B. longum subsp. longum UCD401 (orange), L. plantarum ATCC BAA-793 (blue), and L. johnsonii ATCC 33200 (yellow) after xyloglucan fermentation. The curves are drawn from an average of three independent experiments.

Article Snippet: ATCC 15697 , B. longum subsp. infantis , Human infant feces.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Relationship between total phenolic contents and biological properties of propolis from 20 different regions in South Korea

doi: 10.1186/s12906-016-1043-y

Figure Lengend Snippet: List of human intestinal bacteria tested for growth inhibitory activity

Article Snippet: B. infantis ATCC 25962 b , 2 , P12 (14.7), P19 (14.7), CF d (0.031).

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Relationship between total phenolic contents and biological properties of propolis from 20 different regions in South Korea

doi: 10.1186/s12906-016-1043-y

Figure Lengend Snippet: Minimal inhibitory concentrations (MICs) of Korean propolis ethanol extracts and a commercial antibiotic ciprofloxacin toward four harmful and two nonpathogenic intestinal bacteria

Article Snippet: B. infantis ATCC 25962 b , 2 , P12 (14.7), P19 (14.7), CF d (0.031).

Techniques: