eggerthella lenta  (ATCC)


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    ATCC eggerthella lenta
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    Eggerthella Lenta, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Filifactor alocis - involvement in periodontal biofilms"

    Article Title: Filifactor alocis - involvement in periodontal biofilms

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-10-66

    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), Eggerthella lenta (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    Figure Legend Snippet: Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), Eggerthella lenta (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.

    Techniques Used: Dot Blot, Amplification, Cell Culture

    b atcc 25559 atcc 43185 cl03t12c61 hm 728 cre21 dsm 14838 atcc  (ATCC)


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    ATCC b atcc 25559 atcc 43185 cl03t12c61 hm 728 cre21 dsm 14838 atcc
    B Atcc 25559 Atcc 43185 Cl03t12c61 Hm 728 Cre21 Dsm 14838 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lenta atcc 25559 type strain reconstruction  (ATCC)


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    ATCC lenta atcc 25559 type strain reconstruction
    Lenta Atcc 25559 Type Strain Reconstruction, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    25559 version  (ATCC)


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    ATCC 25559 version
    25559 Version, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eggerthella lenta  (ATCC)


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    Structured Review

    ATCC eggerthella lenta
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    Eggerthella Lenta, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eggerthella lenta/product/ATCC
    Average 93 stars, based on 1 article reviews
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    eggerthella lenta - by Bioz Stars, 2023-09
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    1) Product Images from "Filifactor alocis - involvement in periodontal biofilms"

    Article Title: Filifactor alocis - involvement in periodontal biofilms

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-10-66

    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), Eggerthella lenta (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    Figure Legend Snippet: Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), Eggerthella lenta (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.

    Techniques Used: Dot Blot, Amplification, Cell Culture

    eggerthella lenta  (ATCC)


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    ATCC eggerthella lenta
    Cross-reactivity and inclusivity panel. Only Mycoplasma hominis, Ureaplasma parvum , and Ureaplasma urealyticum were detected (by the appropriate assays).
    Eggerthella Lenta, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Rapid PCR Detection of Mycoplasma hominis , Ureaplasma urealyticum , and Ureaplasma parvum"

    Article Title: Rapid PCR Detection of Mycoplasma hominis , Ureaplasma urealyticum , and Ureaplasma parvum

    Journal: International Journal of Bacteriology

    doi: 10.1155/2013/168742

    Cross-reactivity and inclusivity panel. Only Mycoplasma hominis, Ureaplasma parvum , and Ureaplasma urealyticum were detected (by the appropriate assays).
    Figure Legend Snippet: Cross-reactivity and inclusivity panel. Only Mycoplasma hominis, Ureaplasma parvum , and Ureaplasma urealyticum were detected (by the appropriate assays).

    Techniques Used:

    25559 version  (ATCC)


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    ATCC 25559 version
    25559 Version, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    e lenta atcc 25559 type strain reconstruction  (ATCC)


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    ATCC e lenta atcc 25559 type strain reconstruction
    E Lenta Atcc 25559 Type Strain Reconstruction, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e lenta  (ATCC)


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    ATCC e lenta
    E Lenta, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eubacterium lentum atcc no 25559  (ATCC)


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    ATCC eubacterium lentum atcc no 25559
    Eubacterium Lentum Atcc No 25559, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eggerthella lenta atcc 25559  (ATCC)


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    Structured Review

    ATCC eggerthella lenta atcc 25559
    Eggerthella Lenta Atcc 25559, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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  • 93
    ATCC eggerthella lenta
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    Eggerthella Lenta, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC b atcc 25559 atcc 43185 cl03t12c61 hm 728 cre21 dsm 14838 atcc
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    B Atcc 25559 Atcc 43185 Cl03t12c61 Hm 728 Cre21 Dsm 14838 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b atcc 25559 atcc 43185 cl03t12c61 hm 728 cre21 dsm 14838 atcc - by Bioz Stars, 2023-09
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    86
    ATCC lenta atcc 25559 type strain reconstruction
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    Lenta Atcc 25559 Type Strain Reconstruction, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC 25559 version
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    25559 Version, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC e lenta atcc 25559 type strain reconstruction
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    E Lenta Atcc 25559 Type Strain Reconstruction, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC e lenta
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    E Lenta, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC eubacterium lentum atcc no 25559
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
    Eubacterium Lentum Atcc No 25559, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC eggerthella lenta atcc 25559
    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), <t>Eggerthella</t> <t>lenta</t> (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.
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    Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), Eggerthella lenta (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.

    Journal: BMC Microbiology

    Article Title: Filifactor alocis - involvement in periodontal biofilms

    doi: 10.1186/1471-2180-10-66

    Figure Lengend Snippet: Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b) . PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), Eggerthella lenta (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.

    Article Snippet: In addition, fixed cells of 16 different bacterial species, most of them periodontal pathogens, were incubated with FIAL at 20% (v/v) formamide as negative controls, namely F. nucleatum (ATCC 25586), Eikenella corrodens (CCUG 2138), Kingella kingae (ATCC 23330), Veillonella parvula (ATCC 10790), Veillonella dispar (ATCC 17748), P. gingivalis (ATCC 33277), A. actinomycetemcomitans (ATCC 33384), Pasteurella haemolytica (ATCC 33396), T. forsythia (ATCC 43037), Haemophilus aphrophilus (NCTC 55906) P. intermedia (ATCC 25611), Campylobacter rectus (ATCC 33238), Capnocytophaga sputigena (ATCC 33612), Capnocytophaga gingivalis (ATCC 33624), Eggerthella lenta (ATCC 25559), and Peptostreptococcus anaerobius (ATCC 27337).

    Techniques: Dot Blot, Amplification, Cell Culture