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atcc 25464  (ATCC)


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    ATCC atcc 25464
    Atcc 25464, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atcc 25464/product/ATCC
    Average 90 stars, based on 1 article reviews
    atcc 25464 - by Bioz Stars, 2025-07
    90/100 stars

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    Expression of the selected genes in mouse tissues.

    Journal: Scientific Reports

    Article Title: The evolutionarily conserved genes: Tex37 , Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice

    doi: 10.1038/s41598-018-23176-x

    Figure Lengend Snippet: Expression of the selected genes in mouse tissues.

    Article Snippet: Primary antibodies for TEX37 (1:500; Protein tech, 25464-1-AP, USA) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:1000; Millipore, MAB374, MA, USA) were used.

    Techniques: Expressing

    Knockout strategy and genotyping of mutant mice. ( A ) Schematic strategies for the generation of KO mice using CRISPR/Cas9. 44 bp of Tex37 (a), 11 bp and 20 bp of Ccdc73 (b) and 17 bp of Prss55 ( c ) were deleted from Exon 2. While, 18 bp and 32 bp of Lyzl1 (d) and 101 bp of Nxt2 (e) were deleted from exon 3. ( B ) Genotype of each KO mouse was confirmed with PCR. ( C ) Representative Sanger sequence image for the verification of each KO mouse. Red arrow heads above the chromatograms and dashes in aligned cds sequences show the deletions.

    Journal: Scientific Reports

    Article Title: The evolutionarily conserved genes: Tex37 , Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice

    doi: 10.1038/s41598-018-23176-x

    Figure Lengend Snippet: Knockout strategy and genotyping of mutant mice. ( A ) Schematic strategies for the generation of KO mice using CRISPR/Cas9. 44 bp of Tex37 (a), 11 bp and 20 bp of Ccdc73 (b) and 17 bp of Prss55 ( c ) were deleted from Exon 2. While, 18 bp and 32 bp of Lyzl1 (d) and 101 bp of Nxt2 (e) were deleted from exon 3. ( B ) Genotype of each KO mouse was confirmed with PCR. ( C ) Representative Sanger sequence image for the verification of each KO mouse. Red arrow heads above the chromatograms and dashes in aligned cds sequences show the deletions.

    Article Snippet: Primary antibodies for TEX37 (1:500; Protein tech, 25464-1-AP, USA) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:1000; Millipore, MAB374, MA, USA) were used.

    Techniques: Knock-Out, Mutagenesis, CRISPR, Sequencing

    Fertility assay.

    Journal: Scientific Reports

    Article Title: The evolutionarily conserved genes: Tex37 , Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice

    doi: 10.1038/s41598-018-23176-x

    Figure Lengend Snippet: Fertility assay.

    Article Snippet: Primary antibodies for TEX37 (1:500; Protein tech, 25464-1-AP, USA) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:1000; Millipore, MAB374, MA, USA) were used.

    Techniques: