Journal: eNeuro

Article Title: Identification of a Novel Axon Regeneration Role for Noncanonical Wnt Signaling in the Adult Retina after Injury

doi: 10.1523/ENEURO.0182-22.2022

Figure Lengend Snippet: CamKII and JNK signaling is upregulated and PKC signaling is downregulated by Wnt5a. A , Representative Western blot images of phospho and total CamKII, phospho and total JNK, phospho and total PKC, and GAPDH on retinas collected 24 h after Wnt5a or saline injection. B , Normalized phospho protein band intensity with total protein band intensity of CamKII, JNK, and PKC shows significant upregulation of phospho CamKII and JNK in 20 ng Wnt5a-injected retinas compared with saline-injected retinas. In contrast, significant downregulation of phospho PKC was observed in 20 ng Wnt5a-injected retinas compared with the saline-injected retinas ( n = 5 in each group). Mean ± SD is shown. See Extended Data 1 for detailed statistical analyses.

Article Snippet: The membranes were probed with antibodies specific to phospho (βII Ser660) protein kinase C (pPKC; Cell Signaling Technology; 1:1000, #9371S, RRID: AB_2168219 ), phospho (Thr286) CamKII (Cell Signaling Technology, 1:1000, #12716S, RRID: AB_2713889 ), phospho (Thr183/Tyr185)-JNK (Cell Signaling Technology, 1:1000, #4668S, RRID: AB_823588 ), total PKC (Cell Signaling Technology, 1:1000, #25453S, RRID: AB_2798904 ), total CamKII (Cell Signaling Technology, 1:1000, #4436S, RRID: AB_10545451 ), total JNK (Cell Signaling Technology, 1:1000, #9252S, RRID: AB_2250373 ), or GAPDH (Cell Signaling Technology, 1:1000, #5174S, RRID: AB_10622025 ) for 24 h at 4°C.

Techniques: Western Blot, Injection

Journal: eNeuro

Article Title: Identification of a Novel Axon Regeneration Role for Noncanonical Wnt Signaling in the Adult Retina after Injury

doi: 10.1523/ENEURO.0182-22.2022

Figure Lengend Snippet: CamKII and JNK signaling is upregulated by Wnt5a and PKC signaling is downregulated by Wnt5a. A , Representative Western blot images of phospho and total CamKII, phospho and total JNK, phospho and total PKC, and GAPDH. B , Normalized phospho protein band intensity with total protein band intensity of CamKII, JNK, and PKC shows significant upregulation of phospho CamKII and JNK in 50 ng Wnt5a-injected retinas compared with saline-injected retinas. In contrast, significant downregulation of phospho PKC was observed in 50 ng Wnt5a-injected retinas compared with the saline-injected retinas ( n = 5 in each group). Mean ± SD is shown. See Extended Data 1 for detailed statistical analyses.

Article Snippet: The membranes were probed with antibodies specific to phospho (βII Ser660) protein kinase C (pPKC; Cell Signaling Technology; 1:1000, #9371S, RRID: AB_2168219 ), phospho (Thr286) CamKII (Cell Signaling Technology, 1:1000, #12716S, RRID: AB_2713889 ), phospho (Thr183/Tyr185)-JNK (Cell Signaling Technology, 1:1000, #4668S, RRID: AB_823588 ), total PKC (Cell Signaling Technology, 1:1000, #25453S, RRID: AB_2798904 ), total CamKII (Cell Signaling Technology, 1:1000, #4436S, RRID: AB_10545451 ), total JNK (Cell Signaling Technology, 1:1000, #9252S, RRID: AB_2250373 ), or GAPDH (Cell Signaling Technology, 1:1000, #5174S, RRID: AB_10622025 ) for 24 h at 4°C.

Techniques: Western Blot, Injection